The Experts below are selected from a list of 300 Experts worldwide ranked by ideXlab platform
Tatsuya Haga - One of the best experts on this subject based on the ideXlab platform.
-
Sequestration of human muscarinic acetylcholine receptor hm1 hm5 subtypes effect of g protein coupled receptor kinases grk2 grk4 grk5 and grk6
Journal of Pharmacology and Experimental Therapeutics, 1998Co-Authors: Hirofumi Tsuga, Eriko Okuno, Kimihiko Kameyama, Tatsuya HagaAbstract:Sequestration of porcine muscarinic acetylcholine receptor m2 subtypes (m2 receptors) expressed in COS-7 cells is facilitated by coexpression of G protein-coupled receptor kinases 2 (GRK2). We examined the effect of coexpression of GRK2, GRK4δ, GRK5 and GRK6 on Sequestration of human m1—m5 receptors expressed in COS-7 cells, which was assessed as loss of [ 3 H]N-methylscopolamine binding activity from the cell surface. Sequestration of m4 receptors as well as m2 receptors was facilitated by coexpression of GRK2 and attenuated by coexpression of the dominant negative form of GRK2 (DN-GRK2). Sequestration of m3 and m5 receptors also was facilitated by coexpression of GRK2 but not affected by coexpression of DN-GRK2. On the other hand, proportions of sequestered m1 receptors were not significantly different with coexpression of GRK2 and DN-GRK2. GRK4δ, GRK5 and GRK6 did not facilitate Sequestration of m1—m5 receptors in COS-7 cells, except that the Sequestration of m2 receptors tended to be facilitated by coexpression of GRK4δ, GRK5 and GRK6. However, coexpression of GRK4δ, GRK5, but not GRK6, in BHK-21 cells facilitated Sequestration of m2, but not m3, receptors. These results indicate that the effect of GRK2 to facilitate receptor Sequestration is not restricted to m2 receptors but is generalized to other muscarinic receptors except m1 receptors and that other kinases, including GRK4δ, GRK5 and endogenous kinase(s) in COS-7 cells, also contribute to Sequestration of m2 and m4 receptors.
-
Sequestration of Human Muscarinic Acetylcholine Receptor hm1—hm5 Subtypes: Effect of G Protein-Coupled Receptor Kinases GRK2, GRK4, GRK5 and GRK6
Journal of Pharmacology and Experimental Therapeutics, 1998Co-Authors: Hirofumi Tsuga, Eriko Okuno, Kimihiko Kameyama, Tatsuya HagaAbstract:Sequestration of porcine muscarinic acetylcholine receptor m2 subtypes (m2 receptors) expressed in COS-7 cells is facilitated by coexpression of G protein-coupled receptor kinases 2 (GRK2). We examined the effect of coexpression of GRK2, GRK4δ, GRK5 and GRK6 on Sequestration of human m1—m5 receptors expressed in COS-7 cells, which was assessed as loss of [ 3 H]N-methylscopolamine binding activity from the cell surface. Sequestration of m4 receptors as well as m2 receptors was facilitated by coexpression of GRK2 and attenuated by coexpression of the dominant negative form of GRK2 (DN-GRK2). Sequestration of m3 and m5 receptors also was facilitated by coexpression of GRK2 but not affected by coexpression of DN-GRK2. On the other hand, proportions of sequestered m1 receptors were not significantly different with coexpression of GRK2 and DN-GRK2. GRK4δ, GRK5 and GRK6 did not facilitate Sequestration of m1—m5 receptors in COS-7 cells, except that the Sequestration of m2 receptors tended to be facilitated by coexpression of GRK4δ, GRK5 and GRK6. However, coexpression of GRK4δ, GRK5, but not GRK6, in BHK-21 cells facilitated Sequestration of m2, but not m3, receptors. These results indicate that the effect of GRK2 to facilitate receptor Sequestration is not restricted to m2 receptors but is generalized to other muscarinic receptors except m1 receptors and that other kinases, including GRK4δ, GRK5 and endogenous kinase(s) in COS-7 cells, also contribute to Sequestration of m2 and m4 receptors.
Hirofumi Tsuga - One of the best experts on this subject based on the ideXlab platform.
-
Sequestration of human muscarinic acetylcholine receptor hm1 hm5 subtypes effect of g protein coupled receptor kinases grk2 grk4 grk5 and grk6
Journal of Pharmacology and Experimental Therapeutics, 1998Co-Authors: Hirofumi Tsuga, Eriko Okuno, Kimihiko Kameyama, Tatsuya HagaAbstract:Sequestration of porcine muscarinic acetylcholine receptor m2 subtypes (m2 receptors) expressed in COS-7 cells is facilitated by coexpression of G protein-coupled receptor kinases 2 (GRK2). We examined the effect of coexpression of GRK2, GRK4δ, GRK5 and GRK6 on Sequestration of human m1—m5 receptors expressed in COS-7 cells, which was assessed as loss of [ 3 H]N-methylscopolamine binding activity from the cell surface. Sequestration of m4 receptors as well as m2 receptors was facilitated by coexpression of GRK2 and attenuated by coexpression of the dominant negative form of GRK2 (DN-GRK2). Sequestration of m3 and m5 receptors also was facilitated by coexpression of GRK2 but not affected by coexpression of DN-GRK2. On the other hand, proportions of sequestered m1 receptors were not significantly different with coexpression of GRK2 and DN-GRK2. GRK4δ, GRK5 and GRK6 did not facilitate Sequestration of m1—m5 receptors in COS-7 cells, except that the Sequestration of m2 receptors tended to be facilitated by coexpression of GRK4δ, GRK5 and GRK6. However, coexpression of GRK4δ, GRK5, but not GRK6, in BHK-21 cells facilitated Sequestration of m2, but not m3, receptors. These results indicate that the effect of GRK2 to facilitate receptor Sequestration is not restricted to m2 receptors but is generalized to other muscarinic receptors except m1 receptors and that other kinases, including GRK4δ, GRK5 and endogenous kinase(s) in COS-7 cells, also contribute to Sequestration of m2 and m4 receptors.
-
Sequestration of Human Muscarinic Acetylcholine Receptor hm1—hm5 Subtypes: Effect of G Protein-Coupled Receptor Kinases GRK2, GRK4, GRK5 and GRK6
Journal of Pharmacology and Experimental Therapeutics, 1998Co-Authors: Hirofumi Tsuga, Eriko Okuno, Kimihiko Kameyama, Tatsuya HagaAbstract:Sequestration of porcine muscarinic acetylcholine receptor m2 subtypes (m2 receptors) expressed in COS-7 cells is facilitated by coexpression of G protein-coupled receptor kinases 2 (GRK2). We examined the effect of coexpression of GRK2, GRK4δ, GRK5 and GRK6 on Sequestration of human m1—m5 receptors expressed in COS-7 cells, which was assessed as loss of [ 3 H]N-methylscopolamine binding activity from the cell surface. Sequestration of m4 receptors as well as m2 receptors was facilitated by coexpression of GRK2 and attenuated by coexpression of the dominant negative form of GRK2 (DN-GRK2). Sequestration of m3 and m5 receptors also was facilitated by coexpression of GRK2 but not affected by coexpression of DN-GRK2. On the other hand, proportions of sequestered m1 receptors were not significantly different with coexpression of GRK2 and DN-GRK2. GRK4δ, GRK5 and GRK6 did not facilitate Sequestration of m1—m5 receptors in COS-7 cells, except that the Sequestration of m2 receptors tended to be facilitated by coexpression of GRK4δ, GRK5 and GRK6. However, coexpression of GRK4δ, GRK5, but not GRK6, in BHK-21 cells facilitated Sequestration of m2, but not m3, receptors. These results indicate that the effect of GRK2 to facilitate receptor Sequestration is not restricted to m2 receptors but is generalized to other muscarinic receptors except m1 receptors and that other kinases, including GRK4δ, GRK5 and endogenous kinase(s) in COS-7 cells, also contribute to Sequestration of m2 and m4 receptors.
Xinhua He - One of the best experts on this subject based on the ideXlab platform.
-
responses of soil carbon pool and soil aggregates associated organic carbon to straw and straw derived biochar addition in a dryland cropping mesocosm system
Agriculture Ecosystems & Environment, 2018Co-Authors: Rong Huang, Sheng Lv, Dong Tian, Xinhua HeAbstract:Abstract How to address soil carbon (C) Sequestration and crop straw recycling is an intractable challenge for agriculture. The application of various agricultural straws (including fresh straw, decomposed straw, straw-derived biochar) to soil alters the soil C pool. In order to understand soil C dynamics and the potential C Sequestration characters after the addition of straw and/or straw-derived biochar, an in-situ mesocosm experiment was conducted under five treatments as (1) no straw and no biochar control (CT), (2) straw addition only (ST), (3) straw with a straw-decay bacterium (STDB), (4) biochar addition only (BC) and (5) a combination of straw with biochar (STBC). Carbon dioxide (CO2) flux from soil, total soil organic C (SOC) and soil labile organic C (LOC), as well as soil aggregate associated organic C have been analyzed within a dryland rape-maize cropping system. The results showed that soil CO2 flux increased with the addition of crop straws (ST, STBC and STDB), but decreased under BC because of a lower LOC under BC, especially microbial biomass C fraction in the LOC. The combined application of STDB increased the percentages of macro-aggregates (>2 mm and 0.25–2 mm). Meanwhile, the decomposition of organic matter was increased, and the CO2 flux was also increased. The 0.053-0.25 mm aggregate under BC had the highest fine intra-aggregate particulate organic C (iPOC), which promoted C Sequestration. However, the higher coarse-iPOC in >2 mm and 0.25–2 mm aggregates under ST and STDB promoted SOC decomposition and also CO2 flux. Compared with all three straw treatments (ST, STBC and STDB), the sole biochar addition reduced CO2 flux while increased net C Sequestration without significant decreases of crop yields and net primary productivity. The sole biochar addition did improve the physical protections for SOC from soil aggregates. The obtained results showed differential responses of soil C pool and aggregates associated organic C to straw and/or straw-derived biochar addition while providing insights into potential soil C Sequestrations or mitigations by using agriculture based organic materials.
Kimihiko Kameyama - One of the best experts on this subject based on the ideXlab platform.
-
Sequestration of human muscarinic acetylcholine receptor hm1 hm5 subtypes effect of g protein coupled receptor kinases grk2 grk4 grk5 and grk6
Journal of Pharmacology and Experimental Therapeutics, 1998Co-Authors: Hirofumi Tsuga, Eriko Okuno, Kimihiko Kameyama, Tatsuya HagaAbstract:Sequestration of porcine muscarinic acetylcholine receptor m2 subtypes (m2 receptors) expressed in COS-7 cells is facilitated by coexpression of G protein-coupled receptor kinases 2 (GRK2). We examined the effect of coexpression of GRK2, GRK4δ, GRK5 and GRK6 on Sequestration of human m1—m5 receptors expressed in COS-7 cells, which was assessed as loss of [ 3 H]N-methylscopolamine binding activity from the cell surface. Sequestration of m4 receptors as well as m2 receptors was facilitated by coexpression of GRK2 and attenuated by coexpression of the dominant negative form of GRK2 (DN-GRK2). Sequestration of m3 and m5 receptors also was facilitated by coexpression of GRK2 but not affected by coexpression of DN-GRK2. On the other hand, proportions of sequestered m1 receptors were not significantly different with coexpression of GRK2 and DN-GRK2. GRK4δ, GRK5 and GRK6 did not facilitate Sequestration of m1—m5 receptors in COS-7 cells, except that the Sequestration of m2 receptors tended to be facilitated by coexpression of GRK4δ, GRK5 and GRK6. However, coexpression of GRK4δ, GRK5, but not GRK6, in BHK-21 cells facilitated Sequestration of m2, but not m3, receptors. These results indicate that the effect of GRK2 to facilitate receptor Sequestration is not restricted to m2 receptors but is generalized to other muscarinic receptors except m1 receptors and that other kinases, including GRK4δ, GRK5 and endogenous kinase(s) in COS-7 cells, also contribute to Sequestration of m2 and m4 receptors.
-
Sequestration of Human Muscarinic Acetylcholine Receptor hm1—hm5 Subtypes: Effect of G Protein-Coupled Receptor Kinases GRK2, GRK4, GRK5 and GRK6
Journal of Pharmacology and Experimental Therapeutics, 1998Co-Authors: Hirofumi Tsuga, Eriko Okuno, Kimihiko Kameyama, Tatsuya HagaAbstract:Sequestration of porcine muscarinic acetylcholine receptor m2 subtypes (m2 receptors) expressed in COS-7 cells is facilitated by coexpression of G protein-coupled receptor kinases 2 (GRK2). We examined the effect of coexpression of GRK2, GRK4δ, GRK5 and GRK6 on Sequestration of human m1—m5 receptors expressed in COS-7 cells, which was assessed as loss of [ 3 H]N-methylscopolamine binding activity from the cell surface. Sequestration of m4 receptors as well as m2 receptors was facilitated by coexpression of GRK2 and attenuated by coexpression of the dominant negative form of GRK2 (DN-GRK2). Sequestration of m3 and m5 receptors also was facilitated by coexpression of GRK2 but not affected by coexpression of DN-GRK2. On the other hand, proportions of sequestered m1 receptors were not significantly different with coexpression of GRK2 and DN-GRK2. GRK4δ, GRK5 and GRK6 did not facilitate Sequestration of m1—m5 receptors in COS-7 cells, except that the Sequestration of m2 receptors tended to be facilitated by coexpression of GRK4δ, GRK5 and GRK6. However, coexpression of GRK4δ, GRK5, but not GRK6, in BHK-21 cells facilitated Sequestration of m2, but not m3, receptors. These results indicate that the effect of GRK2 to facilitate receptor Sequestration is not restricted to m2 receptors but is generalized to other muscarinic receptors except m1 receptors and that other kinases, including GRK4δ, GRK5 and endogenous kinase(s) in COS-7 cells, also contribute to Sequestration of m2 and m4 receptors.
Eriko Okuno - One of the best experts on this subject based on the ideXlab platform.
-
Sequestration of human muscarinic acetylcholine receptor hm1 hm5 subtypes effect of g protein coupled receptor kinases grk2 grk4 grk5 and grk6
Journal of Pharmacology and Experimental Therapeutics, 1998Co-Authors: Hirofumi Tsuga, Eriko Okuno, Kimihiko Kameyama, Tatsuya HagaAbstract:Sequestration of porcine muscarinic acetylcholine receptor m2 subtypes (m2 receptors) expressed in COS-7 cells is facilitated by coexpression of G protein-coupled receptor kinases 2 (GRK2). We examined the effect of coexpression of GRK2, GRK4δ, GRK5 and GRK6 on Sequestration of human m1—m5 receptors expressed in COS-7 cells, which was assessed as loss of [ 3 H]N-methylscopolamine binding activity from the cell surface. Sequestration of m4 receptors as well as m2 receptors was facilitated by coexpression of GRK2 and attenuated by coexpression of the dominant negative form of GRK2 (DN-GRK2). Sequestration of m3 and m5 receptors also was facilitated by coexpression of GRK2 but not affected by coexpression of DN-GRK2. On the other hand, proportions of sequestered m1 receptors were not significantly different with coexpression of GRK2 and DN-GRK2. GRK4δ, GRK5 and GRK6 did not facilitate Sequestration of m1—m5 receptors in COS-7 cells, except that the Sequestration of m2 receptors tended to be facilitated by coexpression of GRK4δ, GRK5 and GRK6. However, coexpression of GRK4δ, GRK5, but not GRK6, in BHK-21 cells facilitated Sequestration of m2, but not m3, receptors. These results indicate that the effect of GRK2 to facilitate receptor Sequestration is not restricted to m2 receptors but is generalized to other muscarinic receptors except m1 receptors and that other kinases, including GRK4δ, GRK5 and endogenous kinase(s) in COS-7 cells, also contribute to Sequestration of m2 and m4 receptors.
-
Sequestration of Human Muscarinic Acetylcholine Receptor hm1—hm5 Subtypes: Effect of G Protein-Coupled Receptor Kinases GRK2, GRK4, GRK5 and GRK6
Journal of Pharmacology and Experimental Therapeutics, 1998Co-Authors: Hirofumi Tsuga, Eriko Okuno, Kimihiko Kameyama, Tatsuya HagaAbstract:Sequestration of porcine muscarinic acetylcholine receptor m2 subtypes (m2 receptors) expressed in COS-7 cells is facilitated by coexpression of G protein-coupled receptor kinases 2 (GRK2). We examined the effect of coexpression of GRK2, GRK4δ, GRK5 and GRK6 on Sequestration of human m1—m5 receptors expressed in COS-7 cells, which was assessed as loss of [ 3 H]N-methylscopolamine binding activity from the cell surface. Sequestration of m4 receptors as well as m2 receptors was facilitated by coexpression of GRK2 and attenuated by coexpression of the dominant negative form of GRK2 (DN-GRK2). Sequestration of m3 and m5 receptors also was facilitated by coexpression of GRK2 but not affected by coexpression of DN-GRK2. On the other hand, proportions of sequestered m1 receptors were not significantly different with coexpression of GRK2 and DN-GRK2. GRK4δ, GRK5 and GRK6 did not facilitate Sequestration of m1—m5 receptors in COS-7 cells, except that the Sequestration of m2 receptors tended to be facilitated by coexpression of GRK4δ, GRK5 and GRK6. However, coexpression of GRK4δ, GRK5, but not GRK6, in BHK-21 cells facilitated Sequestration of m2, but not m3, receptors. These results indicate that the effect of GRK2 to facilitate receptor Sequestration is not restricted to m2 receptors but is generalized to other muscarinic receptors except m1 receptors and that other kinases, including GRK4δ, GRK5 and endogenous kinase(s) in COS-7 cells, also contribute to Sequestration of m2 and m4 receptors.
