The Experts below are selected from a list of 192 Experts worldwide ranked by ideXlab platform
Raphael Schiffmann - One of the best experts on this subject based on the ideXlab platform.
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altered dopamine and Serotonin Metabolism in motorically asymptomatic r6 2 mice
PLOS ONE, 2011Co-Authors: Fanny Mochel, Brandon Durant, Alexandra Durr, Raphael SchiffmannAbstract:The pattern of cerebral dopamine (DA) abnormalities in Huntington disease (HD) is complex, as reflected by the variable clinical benefit of both DA antagonists and agonists in treating HD symptoms. In addition, little is known about Serotonin Metabolism despite the early occurrence of anxiety and depression in HD. Post-mortem enzymatic changes are likely to interfere with the in vivo profile of biogenic amines. Hence, in order to reliably characterize the regional and chronological profile of brain neurotransmitters in a HD mouse model, we used a microwave fixation system that preserves in vivo concentrations of dopaminergic and Serotoninergic amines. DA was decreased in the striatum of R6/2 mice at 8 and 12 weeks of age while DA metabolites, 3-methoxytyramine and homovanillic acid, were already significantly reduced in 4-week-old motorically asymptomatic R6/2 mice. In the striatum, hippocampus and frontal cortex of 4, 8 and 12-week-old R6/2 mice, Serotonin and its metabolite 5-hydroxyindoleacetic acid were significantly decreased in association with a decreased turnover of Serotonin. In addition, automated high-resolution behavioural analyses displayed stress-like behaviours such as jumping and grooming and altered spatial learning in R6/2 mice at age 4 and 6 weeks respectively. Therefore, we describe the earliest alterations of DA and Serotonin Metabolism in a HD murine model. Our findings likely underpin the neuropsychological symptoms at time of disease onset in HD.
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Altered Dopamine and Serotonin Metabolism in Motorically Asymptomatic R6/2 Mice
PLOS ONE, 2011Co-Authors: Fanny Mochel, Brandon Durant, Alexandra Durr, Raphael SchiffmannAbstract:The pattern of cerebral dopamine (DA) abnormalities in Huntington disease (HD) is complex, as reflected by the variable clinical benefit of both DA antagonists and agonists in treating HD symptoms. In addition, little is known about Serotonin Metabolism despite the early occurrence of anxiety and depression in HD. Post-mortem enzymatic changes are likely to interfere with the in vivo profile of biogenic amines. Hence, in order to reliably characterize the regional and chronological profile of brain neurotransmitters in a HD mouse model, we used a microwave fixation system that preserves in vivo concentrations of dopaminergic and Serotoninergic amines. DA was decreased in the striatum of R6/2 mice at 8 and 12 weeks of age while DA metabolites, 3-methoxytyramine and homovanillic acid, were already significantly reduced in 4-week-old motorically asymptomatic R6/2 mice. In the striatum, hippocampus and frontal cortex of 4, 8 and 12-week-old R6/2 mice, Serotonin and its metabolite 5-hydroxyindoleacetic acid were significantly decreased in association with a decreased turnover of Serotonin. In addition, automated high-resolution behavioural analyses displayed stress-like behaviours such as jumping and grooming and altered spatial learning in R6/2 mice at age 4 and 6 weeks respectively. Therefore, we describe the earliest alterations of DA and Serotonin Metabolism in a HD murine model. Our findings likely underpin the neuropsychological symptoms at time of disease onset in HD.
A A M Masclee - One of the best experts on this subject based on the ideXlab platform.
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alterations in Serotonin Metabolism in the irritable bowel syndrome
Alimentary Pharmacology & Therapeutics, 2016Co-Authors: A Y Thijssen, Daniel Keszthelyi, Zlatan Mujagic, Daisy Jonkers, Samefko Ludidi, M Hesselink, C H M Clemens, Jose M Conchillo, Joanna W Kruimel, A A M MascleeAbstract:Summary Background Alterations in Serotonin (5-HT) Metabolism have been postulated to play a role in the pathogenesis of irritable bowel syndrome (IBS). However, previous reports regarding 5-HT Metabolism in IBS are contradicting. Aim To compare platelet poor plasma (PPP) 5-HT and 5-hydroxyindole acetic acid (5-HIAA) levels and their ratio in a large cohort of IBS patients and healthy controls (HC), including IBS-subgroup analysis. Methods Irritable bowel syndrome patients and HC were evaluated for fasting PPP 5-HT and 5-HIAA levels. Furthermore, GI-symptom diary, GSRS, quality of life, anxiety and depression scores were assessed in the 2 weeks before blood sampling. Results One hundred and fifty four IBS patients and 137 HC were included. No differences were detected in plasma 5-HT between groups. The 5-HIAA concentrations and 5-HIAA/5-HT ratio were significantly lower in IBS compared to HC: 24.6 ± 21.9 vs. 39.0 ± 29.5 μg/L (P < 0.001) and 8.4 ± 12.2 vs. 13.5 ± 16.6 (P < 0.01), respectively. Subtype analysis for 5-HIAA showed all IBS subtypes to be significantly different from HC. The 5-HIAA/5-HT ratio was significantly lower in the IBS-M subtype vs. HC. Linear regression analysis points to an influence of gender but not of GI-symptoms, psychological scores or medication use. Conclusions We demonstrated that fasting 5-HT plasma levels are not significantly different in IBS patients compared to controls. However, decreased 5-HIAA levels and 5-HIAA/5-HT ratio in IBS patients may reflect altered Serotonin Metabolism in IBS. Gender affects 5-HIAA levels in IBS patients, but no effects of drugs, such as SSRIs, or higher GI-symptom or psychological scores were found.
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understanding the role of tryptophan and Serotonin Metabolism in gastrointestinal function
Neurogastroenterology and Motility, 2009Co-Authors: Daniel Keszthelyi, Freddy J Troost, A A M MascleeAbstract:Abstract Tryptophan is the precursor of a wide array of metabolites, which are involved in a variety of aspects of human nutrition and Metabolism. Accumulating evidence suggests a role of tryptophan metabolites, especially Serotonin (5-hydroxytryptamin) in intestinal (patho) physiology, although mechanisms of action are still poorly understood. Alterations of Serotonin Metabolism may give rise to gastrointestinal dysfunction. Recently, it has been postulated that other metabolites of tryptophan, mostly of the kynurenine pathway, also play a role in regulating gut function. This review analyses the current knowledge of the interrelationship between tryptophan metabolic pathways and summarizes the existing scientific evidence regarding the role of tryptophan metabolites in intestinal function and in the pathogenesis of gastrointestinal diseases.
Fanny Mochel - One of the best experts on this subject based on the ideXlab platform.
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altered dopamine and Serotonin Metabolism in motorically asymptomatic r6 2 mice
PLOS ONE, 2011Co-Authors: Fanny Mochel, Brandon Durant, Alexandra Durr, Raphael SchiffmannAbstract:The pattern of cerebral dopamine (DA) abnormalities in Huntington disease (HD) is complex, as reflected by the variable clinical benefit of both DA antagonists and agonists in treating HD symptoms. In addition, little is known about Serotonin Metabolism despite the early occurrence of anxiety and depression in HD. Post-mortem enzymatic changes are likely to interfere with the in vivo profile of biogenic amines. Hence, in order to reliably characterize the regional and chronological profile of brain neurotransmitters in a HD mouse model, we used a microwave fixation system that preserves in vivo concentrations of dopaminergic and Serotoninergic amines. DA was decreased in the striatum of R6/2 mice at 8 and 12 weeks of age while DA metabolites, 3-methoxytyramine and homovanillic acid, were already significantly reduced in 4-week-old motorically asymptomatic R6/2 mice. In the striatum, hippocampus and frontal cortex of 4, 8 and 12-week-old R6/2 mice, Serotonin and its metabolite 5-hydroxyindoleacetic acid were significantly decreased in association with a decreased turnover of Serotonin. In addition, automated high-resolution behavioural analyses displayed stress-like behaviours such as jumping and grooming and altered spatial learning in R6/2 mice at age 4 and 6 weeks respectively. Therefore, we describe the earliest alterations of DA and Serotonin Metabolism in a HD murine model. Our findings likely underpin the neuropsychological symptoms at time of disease onset in HD.
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Altered Dopamine and Serotonin Metabolism in Motorically Asymptomatic R6/2 Mice
PLOS ONE, 2011Co-Authors: Fanny Mochel, Brandon Durant, Alexandra Durr, Raphael SchiffmannAbstract:The pattern of cerebral dopamine (DA) abnormalities in Huntington disease (HD) is complex, as reflected by the variable clinical benefit of both DA antagonists and agonists in treating HD symptoms. In addition, little is known about Serotonin Metabolism despite the early occurrence of anxiety and depression in HD. Post-mortem enzymatic changes are likely to interfere with the in vivo profile of biogenic amines. Hence, in order to reliably characterize the regional and chronological profile of brain neurotransmitters in a HD mouse model, we used a microwave fixation system that preserves in vivo concentrations of dopaminergic and Serotoninergic amines. DA was decreased in the striatum of R6/2 mice at 8 and 12 weeks of age while DA metabolites, 3-methoxytyramine and homovanillic acid, were already significantly reduced in 4-week-old motorically asymptomatic R6/2 mice. In the striatum, hippocampus and frontal cortex of 4, 8 and 12-week-old R6/2 mice, Serotonin and its metabolite 5-hydroxyindoleacetic acid were significantly decreased in association with a decreased turnover of Serotonin. In addition, automated high-resolution behavioural analyses displayed stress-like behaviours such as jumping and grooming and altered spatial learning in R6/2 mice at age 4 and 6 weeks respectively. Therefore, we describe the earliest alterations of DA and Serotonin Metabolism in a HD murine model. Our findings likely underpin the neuropsychological symptoms at time of disease onset in HD.
Daniel Keszthelyi - One of the best experts on this subject based on the ideXlab platform.
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alterations in Serotonin Metabolism in the irritable bowel syndrome
Alimentary Pharmacology & Therapeutics, 2016Co-Authors: A Y Thijssen, Daniel Keszthelyi, Zlatan Mujagic, Daisy Jonkers, Samefko Ludidi, M Hesselink, C H M Clemens, Jose M Conchillo, Joanna W Kruimel, A A M MascleeAbstract:Summary Background Alterations in Serotonin (5-HT) Metabolism have been postulated to play a role in the pathogenesis of irritable bowel syndrome (IBS). However, previous reports regarding 5-HT Metabolism in IBS are contradicting. Aim To compare platelet poor plasma (PPP) 5-HT and 5-hydroxyindole acetic acid (5-HIAA) levels and their ratio in a large cohort of IBS patients and healthy controls (HC), including IBS-subgroup analysis. Methods Irritable bowel syndrome patients and HC were evaluated for fasting PPP 5-HT and 5-HIAA levels. Furthermore, GI-symptom diary, GSRS, quality of life, anxiety and depression scores were assessed in the 2 weeks before blood sampling. Results One hundred and fifty four IBS patients and 137 HC were included. No differences were detected in plasma 5-HT between groups. The 5-HIAA concentrations and 5-HIAA/5-HT ratio were significantly lower in IBS compared to HC: 24.6 ± 21.9 vs. 39.0 ± 29.5 μg/L (P < 0.001) and 8.4 ± 12.2 vs. 13.5 ± 16.6 (P < 0.01), respectively. Subtype analysis for 5-HIAA showed all IBS subtypes to be significantly different from HC. The 5-HIAA/5-HT ratio was significantly lower in the IBS-M subtype vs. HC. Linear regression analysis points to an influence of gender but not of GI-symptoms, psychological scores or medication use. Conclusions We demonstrated that fasting 5-HT plasma levels are not significantly different in IBS patients compared to controls. However, decreased 5-HIAA levels and 5-HIAA/5-HT ratio in IBS patients may reflect altered Serotonin Metabolism in IBS. Gender affects 5-HIAA levels in IBS patients, but no effects of drugs, such as SSRIs, or higher GI-symptom or psychological scores were found.
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understanding the role of tryptophan and Serotonin Metabolism in gastrointestinal function
Neurogastroenterology and Motility, 2009Co-Authors: Daniel Keszthelyi, Freddy J Troost, A A M MascleeAbstract:Abstract Tryptophan is the precursor of a wide array of metabolites, which are involved in a variety of aspects of human nutrition and Metabolism. Accumulating evidence suggests a role of tryptophan metabolites, especially Serotonin (5-hydroxytryptamin) in intestinal (patho) physiology, although mechanisms of action are still poorly understood. Alterations of Serotonin Metabolism may give rise to gastrointestinal dysfunction. Recently, it has been postulated that other metabolites of tryptophan, mostly of the kynurenine pathway, also play a role in regulating gut function. This review analyses the current knowledge of the interrelationship between tryptophan metabolic pathways and summarizes the existing scientific evidence regarding the role of tryptophan metabolites in intestinal function and in the pathogenesis of gastrointestinal diseases.
Alexandra Durr - One of the best experts on this subject based on the ideXlab platform.
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altered dopamine and Serotonin Metabolism in motorically asymptomatic r6 2 mice
PLOS ONE, 2011Co-Authors: Fanny Mochel, Brandon Durant, Alexandra Durr, Raphael SchiffmannAbstract:The pattern of cerebral dopamine (DA) abnormalities in Huntington disease (HD) is complex, as reflected by the variable clinical benefit of both DA antagonists and agonists in treating HD symptoms. In addition, little is known about Serotonin Metabolism despite the early occurrence of anxiety and depression in HD. Post-mortem enzymatic changes are likely to interfere with the in vivo profile of biogenic amines. Hence, in order to reliably characterize the regional and chronological profile of brain neurotransmitters in a HD mouse model, we used a microwave fixation system that preserves in vivo concentrations of dopaminergic and Serotoninergic amines. DA was decreased in the striatum of R6/2 mice at 8 and 12 weeks of age while DA metabolites, 3-methoxytyramine and homovanillic acid, were already significantly reduced in 4-week-old motorically asymptomatic R6/2 mice. In the striatum, hippocampus and frontal cortex of 4, 8 and 12-week-old R6/2 mice, Serotonin and its metabolite 5-hydroxyindoleacetic acid were significantly decreased in association with a decreased turnover of Serotonin. In addition, automated high-resolution behavioural analyses displayed stress-like behaviours such as jumping and grooming and altered spatial learning in R6/2 mice at age 4 and 6 weeks respectively. Therefore, we describe the earliest alterations of DA and Serotonin Metabolism in a HD murine model. Our findings likely underpin the neuropsychological symptoms at time of disease onset in HD.
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Altered Dopamine and Serotonin Metabolism in Motorically Asymptomatic R6/2 Mice
PLOS ONE, 2011Co-Authors: Fanny Mochel, Brandon Durant, Alexandra Durr, Raphael SchiffmannAbstract:The pattern of cerebral dopamine (DA) abnormalities in Huntington disease (HD) is complex, as reflected by the variable clinical benefit of both DA antagonists and agonists in treating HD symptoms. In addition, little is known about Serotonin Metabolism despite the early occurrence of anxiety and depression in HD. Post-mortem enzymatic changes are likely to interfere with the in vivo profile of biogenic amines. Hence, in order to reliably characterize the regional and chronological profile of brain neurotransmitters in a HD mouse model, we used a microwave fixation system that preserves in vivo concentrations of dopaminergic and Serotoninergic amines. DA was decreased in the striatum of R6/2 mice at 8 and 12 weeks of age while DA metabolites, 3-methoxytyramine and homovanillic acid, were already significantly reduced in 4-week-old motorically asymptomatic R6/2 mice. In the striatum, hippocampus and frontal cortex of 4, 8 and 12-week-old R6/2 mice, Serotonin and its metabolite 5-hydroxyindoleacetic acid were significantly decreased in association with a decreased turnover of Serotonin. In addition, automated high-resolution behavioural analyses displayed stress-like behaviours such as jumping and grooming and altered spatial learning in R6/2 mice at age 4 and 6 weeks respectively. Therefore, we describe the earliest alterations of DA and Serotonin Metabolism in a HD murine model. Our findings likely underpin the neuropsychological symptoms at time of disease onset in HD.
