The Experts below are selected from a list of 51 Experts worldwide ranked by ideXlab platform
Rongjia Zhou - One of the best experts on this subject based on the ideXlab platform.
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alternative splicing and differential expression of p450c17 cyp17 in gonads during Sex Transformation in the rice field eel
Biochemical and Biophysical Research Communications, 2003Co-Authors: Hanhua Cheng, Laixin Xia, Yiqing Guo, Rongjia ZhouAbstract:Several mechanisms were used in determination of the development of the male or female of vertebrates. The genes for determination of sequential hermaphrodite Sex are unknown. Here, we reported cloning, alternative splicing, and expression patterns of the CYP17 gene of the rice field eel, a teleost fish with a characteristic of nature Sex reversal. The CYP17 gene of the rice field eel was clustered into the CYP17 gene group of all the other vertebrates, especially into the fish subgroup. Four isoforms of the CYP17 were generated in gonads by alternative splicing and polyadenylation. Alternative splicing events of all these isoforms occurred in 3(') regions, which encoded three different sizes (517, 512, and 159aa) of proteins. RT-PCR results indicate specific expression in gonads of these isoforms. Northern blot analysis shows that expression patterns of the CYP17 (dominantly expressed in testis, less in ovary, and the least in ovotestis) are consistent with the Sex reversal process of the rice field eel. In situ hybridization further shows its specific expression in germinal lamellae, the gonadal epithelium of the gonads. These findings indicate that CYP17 is differentially regulated in a Sex- and developmentally specific manner, suggesting that the CYP17 potentially has important roles in gonad differentiation during Sex reversal of the rice field eel.
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molecular cloning and expression of sox17 in gonads during Sex reversal in the rice field eel a teleost fish with a characteristic of natural Sex Transformation
Biochemical and Biophysical Research Communications, 2003Co-Authors: Rui Wang, Hanhua Cheng, Laixin Xia, Yiqing Guo, Xiao Huang, Rongjia ZhouAbstract:The Sox is a large family of genes which encode transcription factors with high-mobility-group DNA binding domain related to the SRY, with diverse roles in development, and a few of them are involved in Sex determination and differentiation. We report here the identification of Sox17 gene of the rice field eel, a teleost fish with a characteristic of natural Sex Transformation. This gene, located on chromosome 5, consists of two exons which encode a 399-amino acid protein with a conserved HMG box. Phylogenetic analysis shows that the rice field eel Sox17 fits within the Sox17 clade of vertebrates. The rice field eel Sox17 was dominantly expressed in gonads of male, female, and interSex, besides in brain and spleen. Gene expression analysis by in situ hybridization showed its expression in testis, ovary, and ovotestis, and specifically in the gonadal lamellae of ovary, ovotestis, and testis and developing spermatogenic cells of testis, suggesting that they have potentially important roles in gonadal differentiation during Sex reversal in this species.
Hanhua Cheng - One of the best experts on this subject based on the ideXlab platform.
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alternative splicing and differential expression of p450c17 cyp17 in gonads during Sex Transformation in the rice field eel
Biochemical and Biophysical Research Communications, 2003Co-Authors: Hanhua Cheng, Laixin Xia, Yiqing Guo, Rongjia ZhouAbstract:Several mechanisms were used in determination of the development of the male or female of vertebrates. The genes for determination of sequential hermaphrodite Sex are unknown. Here, we reported cloning, alternative splicing, and expression patterns of the CYP17 gene of the rice field eel, a teleost fish with a characteristic of nature Sex reversal. The CYP17 gene of the rice field eel was clustered into the CYP17 gene group of all the other vertebrates, especially into the fish subgroup. Four isoforms of the CYP17 were generated in gonads by alternative splicing and polyadenylation. Alternative splicing events of all these isoforms occurred in 3(') regions, which encoded three different sizes (517, 512, and 159aa) of proteins. RT-PCR results indicate specific expression in gonads of these isoforms. Northern blot analysis shows that expression patterns of the CYP17 (dominantly expressed in testis, less in ovary, and the least in ovotestis) are consistent with the Sex reversal process of the rice field eel. In situ hybridization further shows its specific expression in germinal lamellae, the gonadal epithelium of the gonads. These findings indicate that CYP17 is differentially regulated in a Sex- and developmentally specific manner, suggesting that the CYP17 potentially has important roles in gonad differentiation during Sex reversal of the rice field eel.
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molecular cloning and expression of sox17 in gonads during Sex reversal in the rice field eel a teleost fish with a characteristic of natural Sex Transformation
Biochemical and Biophysical Research Communications, 2003Co-Authors: Rui Wang, Hanhua Cheng, Laixin Xia, Yiqing Guo, Xiao Huang, Rongjia ZhouAbstract:The Sox is a large family of genes which encode transcription factors with high-mobility-group DNA binding domain related to the SRY, with diverse roles in development, and a few of them are involved in Sex determination and differentiation. We report here the identification of Sox17 gene of the rice field eel, a teleost fish with a characteristic of natural Sex Transformation. This gene, located on chromosome 5, consists of two exons which encode a 399-amino acid protein with a conserved HMG box. Phylogenetic analysis shows that the rice field eel Sox17 fits within the Sox17 clade of vertebrates. The rice field eel Sox17 was dominantly expressed in gonads of male, female, and interSex, besides in brain and spleen. Gene expression analysis by in situ hybridization showed its expression in testis, ovary, and ovotestis, and specifically in the gonadal lamellae of ovary, ovotestis, and testis and developing spermatogenic cells of testis, suggesting that they have potentially important roles in gonadal differentiation during Sex reversal in this species.
Laixin Xia - One of the best experts on this subject based on the ideXlab platform.
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alternative splicing and differential expression of p450c17 cyp17 in gonads during Sex Transformation in the rice field eel
Biochemical and Biophysical Research Communications, 2003Co-Authors: Hanhua Cheng, Laixin Xia, Yiqing Guo, Rongjia ZhouAbstract:Several mechanisms were used in determination of the development of the male or female of vertebrates. The genes for determination of sequential hermaphrodite Sex are unknown. Here, we reported cloning, alternative splicing, and expression patterns of the CYP17 gene of the rice field eel, a teleost fish with a characteristic of nature Sex reversal. The CYP17 gene of the rice field eel was clustered into the CYP17 gene group of all the other vertebrates, especially into the fish subgroup. Four isoforms of the CYP17 were generated in gonads by alternative splicing and polyadenylation. Alternative splicing events of all these isoforms occurred in 3(') regions, which encoded three different sizes (517, 512, and 159aa) of proteins. RT-PCR results indicate specific expression in gonads of these isoforms. Northern blot analysis shows that expression patterns of the CYP17 (dominantly expressed in testis, less in ovary, and the least in ovotestis) are consistent with the Sex reversal process of the rice field eel. In situ hybridization further shows its specific expression in germinal lamellae, the gonadal epithelium of the gonads. These findings indicate that CYP17 is differentially regulated in a Sex- and developmentally specific manner, suggesting that the CYP17 potentially has important roles in gonad differentiation during Sex reversal of the rice field eel.
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molecular cloning and expression of sox17 in gonads during Sex reversal in the rice field eel a teleost fish with a characteristic of natural Sex Transformation
Biochemical and Biophysical Research Communications, 2003Co-Authors: Rui Wang, Hanhua Cheng, Laixin Xia, Yiqing Guo, Xiao Huang, Rongjia ZhouAbstract:The Sox is a large family of genes which encode transcription factors with high-mobility-group DNA binding domain related to the SRY, with diverse roles in development, and a few of them are involved in Sex determination and differentiation. We report here the identification of Sox17 gene of the rice field eel, a teleost fish with a characteristic of natural Sex Transformation. This gene, located on chromosome 5, consists of two exons which encode a 399-amino acid protein with a conserved HMG box. Phylogenetic analysis shows that the rice field eel Sox17 fits within the Sox17 clade of vertebrates. The rice field eel Sox17 was dominantly expressed in gonads of male, female, and interSex, besides in brain and spleen. Gene expression analysis by in situ hybridization showed its expression in testis, ovary, and ovotestis, and specifically in the gonadal lamellae of ovary, ovotestis, and testis and developing spermatogenic cells of testis, suggesting that they have potentially important roles in gonadal differentiation during Sex reversal in this species.
Yiqing Guo - One of the best experts on this subject based on the ideXlab platform.
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alternative splicing and differential expression of p450c17 cyp17 in gonads during Sex Transformation in the rice field eel
Biochemical and Biophysical Research Communications, 2003Co-Authors: Hanhua Cheng, Laixin Xia, Yiqing Guo, Rongjia ZhouAbstract:Several mechanisms were used in determination of the development of the male or female of vertebrates. The genes for determination of sequential hermaphrodite Sex are unknown. Here, we reported cloning, alternative splicing, and expression patterns of the CYP17 gene of the rice field eel, a teleost fish with a characteristic of nature Sex reversal. The CYP17 gene of the rice field eel was clustered into the CYP17 gene group of all the other vertebrates, especially into the fish subgroup. Four isoforms of the CYP17 were generated in gonads by alternative splicing and polyadenylation. Alternative splicing events of all these isoforms occurred in 3(') regions, which encoded three different sizes (517, 512, and 159aa) of proteins. RT-PCR results indicate specific expression in gonads of these isoforms. Northern blot analysis shows that expression patterns of the CYP17 (dominantly expressed in testis, less in ovary, and the least in ovotestis) are consistent with the Sex reversal process of the rice field eel. In situ hybridization further shows its specific expression in germinal lamellae, the gonadal epithelium of the gonads. These findings indicate that CYP17 is differentially regulated in a Sex- and developmentally specific manner, suggesting that the CYP17 potentially has important roles in gonad differentiation during Sex reversal of the rice field eel.
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molecular cloning and expression of sox17 in gonads during Sex reversal in the rice field eel a teleost fish with a characteristic of natural Sex Transformation
Biochemical and Biophysical Research Communications, 2003Co-Authors: Rui Wang, Hanhua Cheng, Laixin Xia, Yiqing Guo, Xiao Huang, Rongjia ZhouAbstract:The Sox is a large family of genes which encode transcription factors with high-mobility-group DNA binding domain related to the SRY, with diverse roles in development, and a few of them are involved in Sex determination and differentiation. We report here the identification of Sox17 gene of the rice field eel, a teleost fish with a characteristic of natural Sex Transformation. This gene, located on chromosome 5, consists of two exons which encode a 399-amino acid protein with a conserved HMG box. Phylogenetic analysis shows that the rice field eel Sox17 fits within the Sox17 clade of vertebrates. The rice field eel Sox17 was dominantly expressed in gonads of male, female, and interSex, besides in brain and spleen. Gene expression analysis by in situ hybridization showed its expression in testis, ovary, and ovotestis, and specifically in the gonadal lamellae of ovary, ovotestis, and testis and developing spermatogenic cells of testis, suggesting that they have potentially important roles in gonadal differentiation during Sex reversal in this species.
Rui Wang - One of the best experts on this subject based on the ideXlab platform.
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molecular cloning and expression of sox17 in gonads during Sex reversal in the rice field eel a teleost fish with a characteristic of natural Sex Transformation
Biochemical and Biophysical Research Communications, 2003Co-Authors: Rui Wang, Hanhua Cheng, Laixin Xia, Yiqing Guo, Xiao Huang, Rongjia ZhouAbstract:The Sox is a large family of genes which encode transcription factors with high-mobility-group DNA binding domain related to the SRY, with diverse roles in development, and a few of them are involved in Sex determination and differentiation. We report here the identification of Sox17 gene of the rice field eel, a teleost fish with a characteristic of natural Sex Transformation. This gene, located on chromosome 5, consists of two exons which encode a 399-amino acid protein with a conserved HMG box. Phylogenetic analysis shows that the rice field eel Sox17 fits within the Sox17 clade of vertebrates. The rice field eel Sox17 was dominantly expressed in gonads of male, female, and interSex, besides in brain and spleen. Gene expression analysis by in situ hybridization showed its expression in testis, ovary, and ovotestis, and specifically in the gonadal lamellae of ovary, ovotestis, and testis and developing spermatogenic cells of testis, suggesting that they have potentially important roles in gonadal differentiation during Sex reversal in this species.