The Experts below are selected from a list of 351 Experts worldwide ranked by ideXlab platform
Boguslawa Budziszewska - One of the best experts on this subject based on the ideXlab platform.
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the effect of uv filters on the viability of neuroblastoma sh sy5y Cell Line
Neurotoxicology, 2016Co-Authors: żaneta Broniowska, Bartosz Pomierny, Boguslawa Budziszewska, Malgorzata Filip, Irena SmagaAbstract:Topical application of cosmetic products, containing ultraviolet filters (UV filters) are recommended as a protection against sunburns and in order to reduce the risk of skin cancer. However, some UV filters can be absorbed through skin and by consuming contaminated food. Among the chemical UV filters, benzophenone-3 (BP-3), 3-(4-methylbenzylidene)camphor (4-MBC) and 2-ethylhexyl-4-methoxycinnamate (OMC) are absorbed through the skin to the greatest extent. So far, these lipophilic compounds were demonstrated to influence the gonadal and thyroid hormone function, but their effect on central nervous system Cells has not been investigated, yet. In the present study, we investigated the effect of some UV filters on Cell viability and caspase-3 activity in SH-SY5Y Cells. It has been found that benzophenone-2 (BP-2), BP-3, 4-methylbenzophenone (4-MBP) and OMC present in the culture medium for 72h in high concentration (10(-5) and 10(-4)M) and 4-MBC only 10(-4)M produced a significant cytotoxic effect, as determined both by the MTT reduction test and LDH release assay. In contrast to necrotic changes, all tested UV filters increased caspase-3 activity in much lower concentrations (from 10(-8) to 10(-7)M). Proapoptotic properties of the test compounds were positively verified by Hoechst staining. The obtained results indicated that UV filters adversely affected the viability of nerve Cells, most likely by enhancing the process of apoptosis. The most potent effect was exerted by BP-3 and 4-MBC and at concentrations that may be reached in vivo. Since human exposure to UV filters is significant these compound should be taken into consideration as one of the possible factors involved in pathogenesis of neurodegenerative diseases.
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effects of ethylene glycol ethers on Cell viability in the human neuroblastoma sh sy5y Cell Line
Pharmacological Reports, 2010Co-Authors: Magdalena Regulska, Bartosz Pomierny, Andrzej Starek, Boguslawa Budziszewska, Agnieszka Bastakaim, Malgorzata Filip, Wladyslaw LasonAbstract:Abstract Ethylene glycol ethers (EGEs) are a class of chemicals used extensively in the manufacture of a wide range of domestic and industrial products, which may result in human exposure and toxicity. Hematologic and reproductive toxicity of EGEs are well known whereas their action on neuronal Cell viability has not been studied so far. In the present study, we investigated the effects of some EGEs on Cell viability and on the hydrogen peroxide-induced damage in the human neuroblastoma (SH-SY5Y) Cells. It has been found that 2-phenoxyethanol in a concentration-dependent manner (5–25 mM, 24 h) increased the basal and H 2 O 2 -induced lactate dehydrogenase (LDH) release and 3-[4,5-dimethylthiazol-2-yl]2,5-diphenyl tetrazolium bromide (MTT) reduction. 2-Butoxyethanol given alone did not affect LDH release and MTT reduction but concentration-dependently enhanced the cytotoxic effect of H 2 O 2 .2-Isopropoxyethanol significantly and concentration-dependently (1–25 mM) increased the basal LDH release and attenuated MTT reduction, but did not potentiate the cytotoxic effect of H 2 O 2 . Contrary to this, 2-methoxyethanol did not show a cytotoxic effect while 2-ethoxyethanol at high concentrations intensified the hydrogen peroxide action. This study demonstrated that among the EGEs studied, 2-phenoxyethanol showed the most consistent cytotoxic effect on neurons in in vitro conditions and enhanced the hydrogen peroxide action. 2-Isopropoxyethanol had also a potent cytotoxic effect, but it did not enhance the hydrogen peroxide action, whereas 2-butoxyethanol only potentiated cytotoxic effect of H 2 O 2 . It is concluded that the results of the present study should be confirmed in in vivo conditions and that some EGEs, especially 2-phenoxyethanol, 2-butoxyethanol and 2-isopropoxyethanol, may be responsible for initiation or exacerbation of neuronal Cell damage.
C Domenici - One of the best experts on this subject based on the ideXlab platform.
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optimization of pam scaffolds for neural tissue engineering preliminary study on an sh sy5y Cell Line
Tissue Engineering Part A, 2008Co-Authors: Johanna Kullenberg, Federica Rosatini, Giovanni Vozzi, Francesca Bianchi, Arti Ahluwalia, C DomeniciAbstract:Engineering neural tissue is one of the most challenging goals of tissue engineering. Neural tissue is highly complex and possesses an organized three-dimensional (3D) distribution that is essential for tissue function. An optimal scaffold for tissue engineering has to provide this distribution until the Cells are able to activate their normal functions and develop neural connections with the host tissue. Different strategies such as gene therapy and Cell transplantation particularly in retinal tissue have been tested, but so far they have only induced retinal degeneration in animals. The objective of this work was to study neural Cell assembly as a function of scaffold features and surface chemistry for application in retinal tissue engineering using microfabricated patterns with a well-defined geometry. Because retinal neurons are known to be arranged in hexagonal arrays, hexagonal scaffolds of poly(DL-lactide-co-glycolide) acid were fabricated using a pressure-assisted microsyringe (PAM) system. The be...
Johanna Kullenberg - One of the best experts on this subject based on the ideXlab platform.
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optimization of pam scaffolds for neural tissue engineering preliminary study on an sh sy5y Cell Line
Tissue Engineering Part A, 2008Co-Authors: Johanna Kullenberg, Federica Rosatini, Giovanni Vozzi, Francesca Bianchi, Arti Ahluwalia, C DomeniciAbstract:Engineering neural tissue is one of the most challenging goals of tissue engineering. Neural tissue is highly complex and possesses an organized three-dimensional (3D) distribution that is essential for tissue function. An optimal scaffold for tissue engineering has to provide this distribution until the Cells are able to activate their normal functions and develop neural connections with the host tissue. Different strategies such as gene therapy and Cell transplantation particularly in retinal tissue have been tested, but so far they have only induced retinal degeneration in animals. The objective of this work was to study neural Cell assembly as a function of scaffold features and surface chemistry for application in retinal tissue engineering using microfabricated patterns with a well-defined geometry. Because retinal neurons are known to be arranged in hexagonal arrays, hexagonal scaffolds of poly(DL-lactide-co-glycolide) acid were fabricated using a pressure-assisted microsyringe (PAM) system. The be...
Lucas Kich Grun - One of the best experts on this subject based on the ideXlab platform.
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choLinergic differentiation of human neuroblastoma sh sy5y Cell Line and its potential use as an in vitro model for alzheimer s disease studies
Molecular Neurobiology, 2019Co-Authors: Liana Marengo De Medeiros, Marco Antonio De Bastiani, Eduardo Pacheco Rico, Patricia Schonhofen, Bianca Pfaffenseller, Bianca Wollenhauptaguiar, Lucas Kich GrunAbstract:ChoLinergic transmission is critical to high-order brain functions such as memory, learning, and attention. Alzheimer’s disease (AD) is characterized by cognitive decLine associated with a specific degeneration of choLinergic neurons. No effective treatment to prevent or reverse the symptoms is known. Part of this might be due to the lack of in vitro models that effectively mimic the relevant features of AD. Here, we describe the characterization of an AD in vitro model using the SH-SY5Y Cell Line. Exponentially growing Cells were maintained in DMEM/F12 medium and differentiation was triggered by the combination of retinoic acid (RA) and BDNF. Both acetylchoLinesterase (AChE) and choLine acetyltransferase (ChAT) enzymatic activities and immunocontent were determined. For mimicking tau and amyloid-β pathology, RA + BDNF-differentiated Cells were challenged with okadaic acid (OA) or soluble oligomers of amyloid-β (AβOs) and neurotoxicity was evaluated. RA + BDNF-induced differentiation resulted in remarkable neuronal morphology alterations characterized by increased neurite density. Enhanced expression and enzymatic activities of choLinergic markers were observed compared to RA-differentiation only. Combination of sublethal doses of AβOs and OA resulted in decreased neurite densities, an in vitro marker of synaptopathy. Challenging RA + BDNF-differentiated SH-SY5Y Cells with the combination of sublethal doses of OA and AβO, without causing considerable decrease of Cell viability, provides an in vitro model which mimics the early-stage pathophysiology of choLinergic neurons affected by AD.
Liana Marengo De Medeiros - One of the best experts on this subject based on the ideXlab platform.
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choLinergic differentiation of human neuroblastoma sh sy5y Cell Line and its potential use as an in vitro model for alzheimer s disease studies
Molecular Neurobiology, 2019Co-Authors: Liana Marengo De Medeiros, Marco Antonio De Bastiani, Eduardo Pacheco Rico, Patricia Schonhofen, Bianca Pfaffenseller, Bianca Wollenhauptaguiar, Lucas Kich GrunAbstract:ChoLinergic transmission is critical to high-order brain functions such as memory, learning, and attention. Alzheimer’s disease (AD) is characterized by cognitive decLine associated with a specific degeneration of choLinergic neurons. No effective treatment to prevent or reverse the symptoms is known. Part of this might be due to the lack of in vitro models that effectively mimic the relevant features of AD. Here, we describe the characterization of an AD in vitro model using the SH-SY5Y Cell Line. Exponentially growing Cells were maintained in DMEM/F12 medium and differentiation was triggered by the combination of retinoic acid (RA) and BDNF. Both acetylchoLinesterase (AChE) and choLine acetyltransferase (ChAT) enzymatic activities and immunocontent were determined. For mimicking tau and amyloid-β pathology, RA + BDNF-differentiated Cells were challenged with okadaic acid (OA) or soluble oligomers of amyloid-β (AβOs) and neurotoxicity was evaluated. RA + BDNF-induced differentiation resulted in remarkable neuronal morphology alterations characterized by increased neurite density. Enhanced expression and enzymatic activities of choLinergic markers were observed compared to RA-differentiation only. Combination of sublethal doses of AβOs and OA resulted in decreased neurite densities, an in vitro marker of synaptopathy. Challenging RA + BDNF-differentiated SH-SY5Y Cells with the combination of sublethal doses of OA and AβO, without causing considerable decrease of Cell viability, provides an in vitro model which mimics the early-stage pathophysiology of choLinergic neurons affected by AD.
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evaluation of the neurotoxic neuroprotective role of organoselenides using differentiated human neuroblastoma sh sy5y Cell Line challenged with 6 hydroxydopamine
Neurotoxicity Research, 2012Co-Authors: Fernanda Martins Lopes, Giovana Ferreira Londero, Liana Marengo De Medeiros, Leonardo Lisboa Da Motta, Guilherme Antonio Behr, Valeska Aguiar De OliveiraAbstract:It is well established that oxidative stress plays a major role in several neurodegenerative conditions, like Parkinson disease (PD). Hence, there is an enormous effort for the development of new antioxidants compounds with therapeutic potential for the management of PD, such as synthetic organoselenides molecules. In this study, we selected between nine different synthetic organoselenides the most eligible ones for further neuroprotection assays, using the differentiated human neuroblastoma SH-SY5Y Cell Line as in vitro model. Neuronal differentiation of exponentially growing human neuroblastoma SH-SY5Y Cells was triggered by cultivating Cells with DMEM/F12 medium with 1% of fetal bovine serum (FBS) with the combination of 10 μM retinoic acid for 7 days. Differentiated Cells were further incubated with different concentrations of nine organoselenides (0.1, 0.3, 3, 10, and 30 μM) for 24 h and Cell viability, neurites densities and the immunocontent of neuronal markers were evaluated. Peroxyl radical scavenging potential of each compound was determined with TRAP assay. Three organoselenides tested presented low cytotoxicity and high antioxidant properties. Pre-treatment of Cells with those compounds for 24 h lead to a significantly neuroprotection against 6-hydroxydopamine (6-OHDA) toxicity, which were directly related to their antioxidant properties. Neuroprotective activity of all three organoselenides was compared to diphenyl diselenide (PhSe)2, the simplest of the diaryl diselenides tested. Our results demonstrate that differentiated human SH-SY5Y Cells are suitable Cellular model to evaluate neuroprotective/neurotoxic role of compounds, and support further evaluation of selected organoselenium molecules as potential pharmacological and therapeutic drugs in the treatment of PD.