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Romero Arias, Angie Carolina - One of the best experts on this subject based on the ideXlab platform.
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Efecto del 6-Shogaol sobre la captación de glucosa y supervivencia de células tumorales derivadas de fibrosarcoma humano (HT1080)
'Universidad Nacional Hermilio Valdizan - Facultad de Ciencias de la Educacion', 2019Co-Authors: Romero Arias, Angie CarolinaAbstract:El jengibre ha sido usado en la medicina tradicional china para tratar múltiples enfermedades dentro de las cuales se encuentra el cáncer. El 6-Shogaol es posiblemente uno de sus compuestos con mayor capacidad antitumoral. Sin embargo, el mecanismo molecular a través del cual el 6-Shogaol ejerce su efecto es aún desconocido. Este trabajo evaluó el efecto del 6-Shogaol sobre la captación de glucosa y supervivencia de células tumorales derivadas de fibrosarcoma humano (HT1080). Para tal fin, se determinó la viabilidad celular, producción de ROS, captación de glucosa y expresión de proteínas de señalización de la vía PI3K/Akt/mTOR. Como control celular se usó fibroblastos de ligamento periodontal (HPdLF). Se observó una disminución de la viabilidad celular y aumento de la producción de ROS de forma dosis dependiente, la cual fue atenuada en presencia N-Acetil Cisteína, lo que también recuperó la viabilidad celular. Así mismo, la captación de glucosa disminuyó con el aumento de las concentraciones de 6-Shogaol y aumentó la expresión de mTOR-p y Akt-p; PTEN se mantuvo activo a todas las concentraciones de 6-Shogaol. Por lo anterior, se puede considerar que el aumento en la producción de ROS -ocasionado por el 6-Shogaol- está asociado con muerte celular, probablemente debido a la disminución de la disponibilidad de uno de los nutrientes principales para la célula tumoral como lo es la glucosa; por otra parte, el aumento en la expresión de proteínas como Akt y mTOR también se relaciona con la muerte celular inducida por ROS.Ginger has been used in traditional Chinese medicine to treat multiple as a cancer. 6-Shogaol is possibly one of its compounds with greater antitumor capacity. However, molecular mechanism through which 6-Shogaol exerts its effect is still unknown. This work evaluated the effect of 6-Shogaol on glucose uptake and survival of tumor cells derived from human fibrosarcoma (HT1080). For this, cell viability, ROS production, glucose uptake and signaling protein expression of the PI3K / Akt / mTOR pathway were determined. Periodontal ligament fibroblasts (HPdLF) were used as cellular control. A decrease in cell viability and increase in ROS production was observed in a dose-dependent manner, which was attenuated in the presence of N-Acetyl Cysteine, which also recovered cell viability. Likewise, glucose uptake decreased with increasing concentrations of 6-Shogaol and increased the expression of mTOR-p and Akt-p; PTEN remained active at all concentrations of 6-Shogaol. Therefore, it can be considered that the increase in the production of ROS-caused by 6-Shogaol- is associated with cell death, probably due to the decrease in the availability of one of the main nutrients for the tumor cell as it is the glucose; On the other hand, the increase in the expression of proteins such as Akt and mTOR is also related to cell death induced by ROS
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Efecto del 6-Shogaol sobre la captación de glucosa y supervivencia de células tumorales derivadas de fibrosarcoma humano (HT1080)
'Universidad Nacional Hermilio Valdizan - Facultad de Ciencias de la Educacion', 2019Co-Authors: Romero Arias, Angie CarolinaAbstract:El jengibre ha sido usado en la medicina tradicional china para tratar múltiples enfermedades dentro de las cuales se encuentra el cáncer. El 6-Shogaol es posiblemente uno de sus compuestos con mayor capacidad antitumoral. Sin embargo, el mecanismo molecular a través del cual el 6-Shogaol ejerce su efecto es aún desconocido. Este trabajo evaluó el efecto del 6-Shogaol sobre la captación de glucosa y supervivencia de células tumorales derivadas de fibrosarcoma humano (HT1080). Para tal fin, se determinó la viabilidad celular, producción de ROS, captación de glucosa y expresión de proteínas de señalización de la vía PI3K/Akt/mTOR. Como control celular se usó fibroblastos de ligamento periodontal (HPdLF). Se observó una disminución de la viabilidad celular y aumento de la producción de ROS de forma dosis dependiente, la cual fue atenuada en presencia N-Acetil Cisteína, lo que también recuperó la viabilidad celular. Así mismo, la captación de glucosa disminuyó con el aumento de las concentraciones de 6-Shogaol y aumentó la expresión de mTOR-p y Akt-p; PTEN se mantuvo activo a todas las concentraciones de 6-Shogaol. Por lo anterior, se puede considerar que el aumento en la producción de ROS -ocasionado por el 6-Shogaol- está asociado con muerte celular, probablemente debido a la disminución de la disponibilidad de uno de los nutrientes principales para la célula tumoral como lo es la glucosa; por otra parte, el aumento en la expresión de proteínas como Akt y mTOR también se relaciona con la muerte celular inducida por ROS.Ginger has been used in traditional Chinese medicine to treat multiple as a cancer. 6-Shogaol is possibly one of its compounds with greater antitumor capacity. However, molecular mechanism through which 6-Shogaol exerts its effect is still unknown. This work evaluated the effect of 6-Shogaol on glucose uptake and survival of tumor cells derived from human fibrosarcoma (HT1080). For this, cell viability, ROS production, glucose uptake and signaling protein expression of the PI3K / Akt / mTOR pathway were determined. Periodontal ligament fibroblasts (HPdLF) were used as cellular control. A decrease in cell viability and increase in ROS production was observed in a dose-dependent manner, which was attenuated in the presence of N-Acetyl Cysteine, which also recovered cell viability. Likewise, glucose uptake decreased with increasing concentrations of 6-Shogaol and increased the expression of mTOR-p and Akt-p; PTEN remained active at all concentrations of 6-Shogaol. Therefore, it can be considered that the increase in the production of ROS-caused by 6-Shogaol- is associated with cell death, probably due to the decrease in the availability of one of the main nutrients for the tumor cell as it is the glucose; On the other hand, the increase in the expression of proteins such as Akt and mTOR is also related to cell death induced by ROS.Magíster en Ciencias BiológicasMaestrí
Hafidz Muchamad - One of the best experts on this subject based on the ideXlab platform.
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PENGARUH SUHU EKSTRAKSI RIMPANG JAHE TERHADAP HASIL KADAR GINGEROL DAN Shogaol MENGGUNAKAN EKSTRAKTOR BERPENGADUK (The Effect of Temperature of Ginger Rhizome Extraction on The Results of Gingerol and Shogaol Content by Using Stirred Extractor)
2018Co-Authors: Hafidz MuchamadAbstract:Pengaruh Suhu Ekstraksi Rimpang Jahe terhadap Hasil Kadar Gingerol dan Shogaol Menggunakan Ekstraktor Berpengaduk Ekstraksi merupakan proses pemisahan dan isolasi zat dari suatu zat dengan penambahan pelarut tertentu untuk mengeluarkan komponen campuran dari zat padat atau zat cair. Pada penelitian ini dilakukan ekstraksi kandungan gingerol dan Shogaol dari rimpang jahe untuk mengetahui pengaruh jumlah pelarut dan suhu ekstraksi terhadap hasil kadar gingerol dan Shogaol. Ekstraksi kandungan gingerol dan Shogaol dilakukan dengan alat ekstraktor berpengaduk yaitu dengan menggunakan bahan serbuk rimpang jahe sebanyak 500 gram untuk setiap percobaan kemudian diekstraksi dengan pelarut air sebanyak 4 liter pada setiap percobaannya. Karakteristik ekstrak gingerol dan Shogaol dianalisa menggunakan spektrofotometer untuk mengetahui nilai absorbansi gingerol dan Shogaol dalam suatu variabel ekstrak. Pengaruh suhu ekstraksi terhadap hasil kadar gingerol dan Shogaol yang optimum yaitu pada percobaan III sebesar 2,844% dengan menggunakan jumlah pelarut 4 liter air, pH= 6, dan suhu 100°C, dimana semakin meningkatnya suhu maka difusi yang terjadi akan semakin besar, sehingga kontak antara solvent dengan solute akan semakin cepat dan proses pelarutan kandungan gingerol dan Shogaol oleh solvent akan terus terjadi sampai solvent jenuh terhadap solute. Berdasarkan hasil praktikum yang telah dilakukan dapat disimpulkan bahwa suhu ekstraksi mengalami kenaikan yang signifikan terhadap hasil kadar gingerol dan Shogaol. Kata Kunci: Ekstraksi, Rimpang Jahe, Gingerol, Shogaol, Spectrofotometer, Suhu The Effect of Temperature of Ginger Rhizome Extraction on The Results of Gingerol and Shogaol Content by Using Stirred Extractor Extraction is the process of separating and isolating substances from a substance by adding certain solvents to remove a mixture of solid or liquid substances. In this study extraction of gingerol and Shogaol content from ginger rhizome was conducted to determine the effect of solvent amount and extraction temperature on the results of gingerol and Shogaol levels. Extraction of gingerol and Shogaol content was carried out with a stirred extractor device using 500 grams of ginger rhizome powder for each experiment and then extracted with 4 liters of water in each experiment. The characteristics of gingerol and Shogaol extract were analyzed using a spectrophotometer to determine the absorbance value of gingerol and Shogaol in an extract variable. The effect of extraction temperature on the optimum results of gingerol and Shogaol levels is in experiment III of 2.844% by using the amount of solvent 4 liters of water, pH = 6, and temperature of 100 ° C, where the temperature increases the greater the diffusion, so contact between the solvent and the solute will be faster and the dissolution process of gingerol and Shogaol content by the solvent will continue to occur until the solvent is saturated with the solute. Based on the results of the practicum that has been carried out, it can be concluded that the extraction temperature has a significant increase in the yield of gingerol and Shogaol. Keywords: Extraction, Ginger Rhizome, Gingerol, Shogaol, Spectrophotometer, Temperatur
Syafa Nur Pangesti - One of the best experts on this subject based on the ideXlab platform.
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pengaruh jumlah pelarut dan suhu ekstraksi rimpang jahe terhadap hasil kadar gingerol dan Shogaol menggunakan ekstraktor berpengaduk the effect of solvent amount and temperature of ginger rhizome extraction on the results of gingerol and Shogaol cont
2018Co-Authors: Syafa Nur PangestiAbstract:Ekstraksi merupakan proses pemisahan dan isolasi zat dari suatu zat dengan penambahan pelarut tertentu untuk mengeluarkan komponen campuran dari zat padat atau zat cair. Pada penelitian ini dilakukan ekstraksi kandungan gingerol dan Shogaol dari rimpang jahe untuk mengetahui pengaruh jumlah pelarut dan suhu ekstraksi terhadap hasil kadar gingerol dan Shogaol. Ekstraksi kandungan gingerol dan Shogaol dilakukan dengan alat ekstraktor berpengaduk yaitu dengan menggunakan bahan serbuk rimpang jahe sebanyak 500 gram untuk setiap percobaan kemudian diekstraksi dengan pelarut air sebanyak 4 liter untuk percobaan I, 6 liter untuk percobaan II, dan 8 liter untuk percobaan III. Karakteristik ekstrak gingerol dan Shogaol dianalisa menggunakan spektrofotometer untuk mengetahui nilai absorbansi gingerol dan Shogaol dalam suatu variabel ekstrak. Pengaruh jumlah pelarut terhadap hasil kadar gingerol dan Shogaol yang optimum yaitu pada percobaan III sebesar 31,358% dengan menggunakan jumlah pelarut 8 liter air, pH= 6, dan suhu 100°C, dimana semakin bertambahnya jumlah pelarut maka distribusi partikel dalam pelarut semakin menyebar sehingga memperluas bidang kontak sehingga menghasilkan kadar gingerol dan Shogaol lebih banyak. Pengaruh suhu ekstraksi terhadap hasil kadar gingerol dan Shogaol yang optimum yaitu pada percobaan III sebesar 18,196% dengan menggunakan jumlah pelarut 4 liter air, pH= 6, dan suhu 100°C, dimana semakin meningkatnya suhu maka difusi yang terjadi akan semakin besar, sehingga kontak antara solvent dengan solute akan semakin cepat dan proses pelarutan kandungan gingerol dan Shogaol oleh solvent akan terus terjadi sampai solvent jenuh terhadap solute. Berdasarkan hasil praktikum yang telah dilakukan dapat disimpulkan bahwa waktu tidak signifikan terhadap hasil kadar gingerol dan Shogaol, sedangkan jumlah pelarut dan suhu ekstraksi mengalami kenaikan yang signifikan terhadap hasil kadar gingerol dan Shogaol. Hasil experimental variabel yang paling berpengaruh terhadap kadar Gingerol dan Shogaol yang dihasilkan adalah jumlah pelarut dan suhu, dapat dilihat dari grafik dengan persamaan y=14,201x + 1,1346 untuk menit ke 30 dan y=14,207x + 1,1379 untuk menit ke 60. Kata Kunci: Ekstraksi, Rimpang Jahe, Gingerol, Shogaol, Spectrofotometer ABSTRACT Extraction is the process of separating and isolating substances from a substance by the addition of a particular solvent to remove a mixed component of a solid or a liquid. In this study, extraction of gingerol and Shogaol content of ginger rhizome to determine the effect of solvent amount and temperature of extraction on the results of levels of gingerol and Shogaol. Extraction of gingerol and Shogaol content was carried out with a stirred extractor device using 500 grams of ginger rhizome powder for each experiment and then extracted with 4 liters of water for experiment I, 6 liters for experiment II, and 8 liters for experiment III. The characteristics of gingerol and Shogaol extract were analyzed using a spectrophotometer to determine the absorbance value of gingerol and Shogaol in an extract variable. The effect of the optimum amount of solvent on the results of the levels of gingerol and Shogaol in experiment III was 31,358% by using the amount of solvent 8 liters of water, pH= 6, and temperature of 100°C, where the amount of solvent increases, the distribution of particles in the solvent spreads more contact area resulting in more levels of gingerol and Shogaol. The effect of extraction temperature on the optimum results of gingerol and Shogaol levels is in the third experiment 18.196% by using a solvent amount of 4 liters of water, pH= 6, and temperature of 100°C, where the temperature increases the greater the diffusion, so contact between the solvent and the solute will be faster and the dissolution process of gingerol and Shogaol content by the solvent will continue to occur until the solvent is saturated with the solute. Based on the results of the practicum that has been done it can be concluded that the time is not significant to the results of gingerol and Shogaol levels, while the amount of solvent and extraction temperature has a significant increase in the results of gingerol and Shogaol levels. Result from the experimental variables that have the most influence on the levels of Gingerol and Shogaol produced is the temperature and the amount of solvent, can be seen from the graph with the equation y = 14.201x + 1.1346 for 30 minutes and y = 14.207x + 1.1379 for minute 60. Keywords: Extraction, Ginger Rhizome, Gingerol, Shogaol, Spectrophotometer
Lu Zhang - One of the best experts on this subject based on the ideXlab platform.
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Thermal activities of 6-gingerol, 8-gingerol and 6-Shogaol on the potentiation of mitochondria thermogenesis based on microcalorimetry
Journal of Thermal Analysis and Calorimetry, 2016Co-Authors: Zhirui Yang, Ding-kun Zhang, Yaming Zhang, Honghong Liu, Xue Han, Quanfu Zheng, Yin-qiu Huang, Chang Chen, Lu ZhangAbstract:Zingiberis Rhizoma is a typical Chinese herb in ‘hot’ property. Nowadays, increasing attention has been aroused for its essential role in both health care and treatment of ‘cold’ syndrome. And research about characteristics of Zingiberis Rhizoma is able to provide an important reference for its deeper understand and successful clinic application. However, cognition level about the ‘hot’ property of Zingiberis Rhizoma is superficial, and mechanism behind the ‘hot’ presentation is still elusive and unclear. In this study, the ‘hot’ property of Zingiberis Rhizoma was investigated at a monomer level with assistance of microcalorimetry, an objective and sensitive method in evaluating biological activity. Three bioactive compounds including 6-gingerol, 8-gingerol and 6-Shogaol were selected, and their thermal activities were explored with mitochondria as target. The power–time curves were recorded, and five thermal parameters (k, Pmax, Tmax, Q and Pav) were obtained. With PCA, Pav was calculated to be the main parameter to measure the bioactivities of 6-gingerol, 8-gingerol and 6-Shogaol. Finally, the bioactivities of three subjects were compared with sequence to be 6-gingerol > 8-gingerol > 6-Shogaol. Generally, our study is promising to offer a reference to the research about characteristics of Zingiberis Rhizoma or other herbs and is of great interest for clinic practice.
Atanas Atanasov - One of the best experts on this subject based on the ideXlab platform.
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Identification and characterization of [6]-Shogaol from ginger as inhibitor of vascular smooth muscle cell proliferation
Molecular Nutrition and Food Research, 2015Co-Authors: Rongxia Liu, Elke Heiss, Nadine Sider, Andreas Schinkovitz, Barbara Gröblacher, Dean Guo, Franz Bucar, Rudolf Bauer, Verena Dirsch, Atanas AtanasovAbstract:Scope Vascular smooth muscle cell (VSMC) proliferation is involved in the pathogenesis of cardiovascular disease, making the identification of new counteracting agents and their mechanisms of action relevant. Ginger and its constituents have been reported to improve cardiovascular health, but no studies exist addressing a potential interference with VSMC proliferation. Methods and results The dichloromethane extract of ginger inhibited VSMC proliferation when monitored by resazurin metabolic conversion (IC50 = 2.5 μg/mL). The examination of major constituents from ginger yielded [6]-Shogaol as the most active compound (IC50 = 2.7 μM). In the tested concentration range [6]-Shogaol did not exhibit cytotoxicity toward VSMC and did not interfere with endothelial cell proliferation. [6]-Shogaol inhibited DNA synthesis and induced accumulation of the VSMC in the G0/G1 cell-cycle phase accompanied with activation of the nuclear factor-erythroid 2-related factor 2 (Nrf2)/HO-1 pathway. Since [6]-Shogaol lost its antiproliferative activity in the presence of the heme oxygenase-1 (HO-1) inhibitor tin protoporphyrin IX, HO-1 induction appears to contribute to the antiproliferative effect. Conclusion This study demonstrates for the first time inhibitory potential of ginger constituents on VSMC proliferation. The presented data suggest that [6]-Shogaol exerts its antiproliferative effect through accumulation of cells in the G0/G1 cell-cycle phase associated with activation of the Nrf2/HO-1 pathway.