The Experts below are selected from a list of 42 Experts worldwide ranked by ideXlab platform
Shunichiro Tsuji - One of the best experts on this subject based on the ideXlab platform.
-
Side Population Cells contribute to the genesis of human endometrium
Fertility and Sterility, 2008Co-Authors: Shunichiro Tsuji, Momoko Yoshimoto, Kentaro Takahashi, Yoichi Noda, Tatsutoshi Nakahata, Toshio HeikeAbstract:Objective To investigate whether stem (progenitor) Cells are found in human endometrial Side Population Cells. Design Experimental laboratory study. Setting University-based laboratory in Japan. Patient(s) Normal endometrial tissue samples from 42 patients. Intervention(s) Side Population Cell analysis and staining were performed by using Hoechst 33342, CD31, CD34, EMA, CD105, CD146, and BCRP1/ABCG2. Main Outcome Measure(s) Human endometrial Side Population Cells were isolated and characterized by fluorescence-activated Cell sorter analysis. Stem Cell activity was evaluated by colony-forming assays and Cell cycle analysis. Result(s) Endometrial Side Population Cells expressed not only the endothelial Cell markers CD31 and CD34 and the epithelial Cell marker EMA but also mesenchymal stem Cell markers CD105 and CD146. Immunohistochemical analysis revealed that BCRP1/ABCG2, known as a marker of Side Population Cells, was strongly expressed in the vascular endothelium and the epithelium of the basal layer of the endometrium. In Cell cycle analysis, Side Population Cells isolated directly from tissue were mainly in G0, whereas Side Population Cells sorted after primary culture included Populations in G1 and G2/M/S. These sorted Side Population Cells showed greater colony-forming efficiency than non-Side Population Cells and secreted PRL in an in vitro decidualization model. Conclusion(s) Human endometrial Side Population Cells may include putative stem or progenitor Cells.
Carlos Simon - One of the best experts on this subject based on the ideXlab platform.
-
reconstruction of endometrium from human endometrial Side Population Cell lines
PLOS ONE, 2011Co-Authors: Irene Cervello, Aymara Mas, Claudia Gilsanchis, Laura Peris, Amparo Faus, Philippa T K Saunders, Hilary O D Critchley, Carlos SimonAbstract:Endometrial regeneration is mediated, at least in part, by the existence of a specialized somatic stem Cell (SSC) Population recently identified by several groups using the Side Population (SP) technique. We previously demonstrated that endometrial SP displays genotypic, phenotypic and the functional capability to develop human endometrium after subcutaneous injection in NOD-SCID mice. We have now established seven human endometrial SP (hESP) Cell lines (ICE 1–7): four from the epithelial and three from the stromal fraction, respectively. SP Cell lines were generated under hypoxic conditions based on their cloning efficiency ability, cultured for 12–15 passages (20 weeks) and cryopreserved. Cell lines displayed normal 46XX karyotype, intermediate telomerase activity pattern and expressed mRNAs encoding proteins that are conSidered characteristic of undifferentiated Cells (Oct-4, GDF3, DNMT3B, Nanog, GABR3) and those of mesodermal origin (WT1, Cardiac Actin, Enolase, Globin, REN). Phenotype analysis corroborated their epithelial (CD9+) or stromal (vimentin+) Cell origin and mesenchymal (CD90+, CD73+ and CD45−) attributes. Markers conSidered characteristic of ectoderm or endoderm were not detected. Cells did not express either estrogen receptor alpha (ERα) or progesterone receptor (PR). The hESP Cell lines were able to differentiate in vitro into adipocytes and osteocytes, which confirmed their mesenchymal origin. Finally, we demonstrated their ability to generate human endometrium when transplanted beneath the renal capsule of NOD-SCID mice. These findings confirm that SP Cells exhibit key features of human endometrial SSC and open up new possibilities for the understanding of gynecological disorders such as endometriosis or Asherman syndrome. Our Cell lines can be a valuable model to investigate new targets for endometrium proliferation in endometriosis.
Ronglih Liao - One of the best experts on this subject based on the ideXlab platform.
-
A role for matrix stiffness in the regulation of cardiac Side Population Cell function
American journal of physiology. Heart and circulatory physiology, 2015Co-Authors: Yiling Qiu, Ahmad F. Bayomy, Marcus V. Gomez, Michael Bauer, Yanfei Yang, Xin Zhang, Ronglih LiaoAbstract:The mechanical properties of the local microenvironment may have important influence on the fate and function of adult tissue progenitor Cells, altering the regenerative process. This is particularly critical following a myocardial infarction, in which the normal, compliant myocardial tissue is replaced with fibrotic, stiff scar tissue. In this study, we examined the effects of matrix stiffness on adult cardiac Side Population (CSP) progenitor Cell behavior. Ovine and murine CSP Cells were isolated and cultured on polydimethylsiloxane substrates, replicating the elastic moduli of normal and fibrotic myocardium. Proliferation capacity and Cell cycling were increased in CSP Cells cultured on the stiff substrate with an associated reduction in cardiomyogeneic differentiation and accelerated Cell ageing. In addition, culture on stiff substrate stimulated upregulation of extraCellular matrix and adhesion proteins gene expression in CSP Cells. Collectively, we demonstrate that microenvironment properties, including matrix stiffness, play a critical role in regulating progenitor Cell functions of endogenous reSident CSP Cells. Understanding the effects of the tissue microenvironment on reSident cardiac progenitor Cells is a critical step toward achieving functional cardiac regeneration.
Yuchen Wang - One of the best experts on this subject based on the ideXlab platform.
-
yap1 regulates abcg2 and cancer Cell Side Population in human lung cancer Cells
Oncotarget, 2017Co-Authors: Yuyuan Dai, Shu Liu, Wenqian Zhang, Yilin Yang, Phillip Hang, Hui Wang, Li Cheng, Pingchih Hsu, Yuchen WangAbstract:// Yuyuan Dai 1, * , Shu Liu 1, * , Wen-Qian Zhang 1, 2 , Yi-Lin Yang 1 , Phillip Hang 1 , Hui Wang 1 , Li Cheng 1 , Ping-Chih Hsu 1 , Yu-Chen Wang 1 , Zhidong Xu 1 , David M. Jablons 1 , Liang You 1 1 Thoracic Oncology Laboratory, Department of Surgery, Comprehensive Cancer Center, University of California, San Francisco, CA, USA 2 Department of Thoracic Surgery, Beijing Chao-Yang Hospital, Affiliated with Capital University of Medical Science, Beijing, People’s Republic of China * These authors contributed equally to this work Correspondence to: Liang You, email: Liang.you@ucsfmedctr.org Keywords: ABCG2, YAP1, Side Population, cancer stem Cells, lung cancer Received: June 04, 2016 Accepted: November 21, 2016 Published: November 29, 2016 ABSTRACT A small Population of cancer Cells called cancer-initiating Cells or cancer stem Cells (CSCs) are involved in drug resistance, metastasis, and cancer relapse. Finding pathways that regulate CSC is very important for clinical therapy. ATP-binding cassette sub-family G member 2 (ABCG2) plays a role in Side Population (SP) Cell formation and contributes to chemotherapy resistance in common forms of cancer. Yes-associated protein 1 (YAP1) is a major transcriptional effector of the Hippo pathway, which plays important roles in organ size control and tumorigenesis. In this study, we found ABCG2 and YAP1 were both overexpressed in lung cancer SP Cells. Disruption of YAP1 expression by siRNA attenuated the expression of ABCG2 transcript and significantly reduced the percentage of SP Cells and sphere formation in lung cancer Cells. Overexpression of YAP1 in lung cancers led to an increase in ABCG2 expression and increased the percentage of SP Cells. However, overexpression of YAP1 in purified non-SP Cells did not increase ABCG2 expression and the percentage of SP Cells, which may be due to the inhibition of YAP activity through phosphorylation. YAP1 directly transcriptionally regulated ABCG2 by binding to the promoter of ABCG2. Moreover, the YAP1 inhibitor verteporfin and YAP1 siRNA downregulated ABCG2 level through inhibition of YAP1 in lung cancer Cells and sensitized them to the chemotherapy drug doxorubicin. Our study adds a new function for YAP1 that may be relevant to drug resistance and cancer therapy through regulation of ABCG2 and Side Population Cell formation in lung cancer.
Toshio Heike - One of the best experts on this subject based on the ideXlab platform.
-
Side Population Cells contribute to the genesis of human endometrium
Fertility and Sterility, 2008Co-Authors: Shunichiro Tsuji, Momoko Yoshimoto, Kentaro Takahashi, Yoichi Noda, Tatsutoshi Nakahata, Toshio HeikeAbstract:Objective To investigate whether stem (progenitor) Cells are found in human endometrial Side Population Cells. Design Experimental laboratory study. Setting University-based laboratory in Japan. Patient(s) Normal endometrial tissue samples from 42 patients. Intervention(s) Side Population Cell analysis and staining were performed by using Hoechst 33342, CD31, CD34, EMA, CD105, CD146, and BCRP1/ABCG2. Main Outcome Measure(s) Human endometrial Side Population Cells were isolated and characterized by fluorescence-activated Cell sorter analysis. Stem Cell activity was evaluated by colony-forming assays and Cell cycle analysis. Result(s) Endometrial Side Population Cells expressed not only the endothelial Cell markers CD31 and CD34 and the epithelial Cell marker EMA but also mesenchymal stem Cell markers CD105 and CD146. Immunohistochemical analysis revealed that BCRP1/ABCG2, known as a marker of Side Population Cells, was strongly expressed in the vascular endothelium and the epithelium of the basal layer of the endometrium. In Cell cycle analysis, Side Population Cells isolated directly from tissue were mainly in G0, whereas Side Population Cells sorted after primary culture included Populations in G1 and G2/M/S. These sorted Side Population Cells showed greater colony-forming efficiency than non-Side Population Cells and secreted PRL in an in vitro decidualization model. Conclusion(s) Human endometrial Side Population Cells may include putative stem or progenitor Cells.