The Experts below are selected from a list of 368322 Experts worldwide ranked by ideXlab platform
Nicholas P Harberd - One of the best experts on this subject based on the ideXlab platform.
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ethylene regulates arabidopsis development via the modulation of della protein growth repressor function
The Plant Cell, 2003Co-Authors: Patrick Achard, Dominique Van Der Straeten, Willem Vriezen, Nicholas P HarberdAbstract:Phytohormones regulate plant development via a poorly understood Signal Response network. Here, we show that the phytohormone ethylene regulates plant development at least in part via alteration of the properties of DELLA protein nuclear growth repressors, a family of proteins first identified as gibberellin (GA) Signaling components. This conclusion is based on the following experimental observations. First, ethylene inhibited Arabidopsis root growth in a DELLA-dependent manner. Second, ethylene delayed the GA-induced disappearance of the DELLA protein repressor of ga1-3 from root cell nuclei via a constitutive triple Response-dependent Signaling pathway. Third, the ethylene-promoted "apical hook" structure of etiolated seedling hypocotyls was dependent on the relief of DELLA-mediated growth restraint. Ethylene, auxin, and GA Responses now can be attributed to effects on DELLA function, suggesting that DELLA plays a key integrative role in the phytohormone Signal Response network.
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ethylene regulates arabidopsis development via the modulation of della protein growth repressor function
The Plant Cell, 2003Co-Authors: Patrick Achard, Willem Vriezen, Dominique Van Der Straeten, Nicholas P HarberdAbstract:Phytohormones regulate plant development via a poorly understood Signal Response network. Here, we show that the phytohormone ethylene regulates plant development at least in part via alteration of the properties of DELLA protein nuclear growth repressors, a family of proteins first identified as gibberellin (GA) Signaling components. This conclusion is based on the following experimental observations. First, ethylene inhibited Arabidopsis root growth in a DELLA-dependent manner. Second, ethylene delayed the GA-induced disappearance of the DELLA protein repressor of ga1-3 from root cell nuclei via a constitutive triple Response-dependent Signaling pathway. Third, the ethylene-promoted “apical hook” structure of etiolated seedling hypocotyls was dependent on the relief of DELLA-mediated growth restraint. Ethylene, auxin, and GA Responses now can be attributed to effects on DELLA function, suggesting that DELLA plays a key integrative role in the phytohormone Signal Response network.
Z. Mester - One of the best experts on this subject based on the ideXlab platform.
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nonlinear Signal Response in electrospray mass spectrometry implications for quantitation of arsenobetaine using stable isotope labeling by liquid chromatography and electrospray orbitrap mass spectrometry
Analytical Chemistry, 2012Co-Authors: L. Ouerdane, J. Meija, S. Bakirdere, L. Yang, Z. MesterAbstract:Isotope amount ratio measurements by electrospray ionization mass spectrometry show large systematic biases. Moreover, the Signal ratio Response can vary nonlinearly with respect to the amount ratio depending on the concentration of the analyte or coeluting matrix components, among other things. Since isotope dilution relies inherently on the linearity of Response, accurate quantitation is then more difficult to achieve. In this study, we outline a method to eliminate the quantitation errors due to the effects of the nonlinear Signal Response. The proposed approach is a hybrid of the method of standard additions and isotope dilution allowing correction for nonlinear trend. As a proof of concept, determination of arsenobetaine content in fish tissue was performed using liquid chromatography coupled with a linear quadrupole ion trap (LTQ) Orbitrap mass spectrometer. The nonlinear isotope dilution method could, in principle, be applied to correct isotope ratio measurement biases in popular relative quantitation methods of biomolecules such as stable isotope labeling by amino acids in cell culture (SILAC), isotope-coded affinity tag (ICAT), or isobaric tags for relative and absolute quantification (iTRAQ).
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Nonlinear Signal Response in Electrospray Mass Spectrometry: Implications for Quantitation of Arsenobetaine Using Stable Isotope Labeling by Liquid Chromatography and Electrospray Orbitrap Mass Spectrometry
2012Co-Authors: L. Ouerdane, J. Meija, S. Bakirdere, L. Yang, Z. MesterAbstract:Isotope amount ratio measurements by electrospray ionization mass spectrometry show large systematic biases. Moreover, the Signal ratio Response can vary nonlinearly with respect to the amount ratio depending on the concentration of the analyte or coeluting matrix components, among other things. Since isotope dilution relies inherently on the linearity of Response, accurate quantitation is then more difficult to achieve. In this study, we outline a method to eliminate the quantitation errors due to the effects of the nonlinear Signal Response. The proposed approach is a hybrid of the method of standard additions and isotope dilution allowing correction for nonlinear trend. As a proof of concept, determination of arsenobetaine content in fish tissue was performed using liquid chromatography coupled with a linear quadrupole ion trap (LTQ) Orbitrap mass spectrometer. The nonlinear isotope dilution method could, in principle, be applied to correct isotope ratio measurement biases in popular relative quantitation methods of biomolecules such as stable isotope labeling by amino acids in cell culture (SILAC), isotope-coded affinity tag (ICAT), or isobaric tags for relative and absolute quantification (iTRAQ)
Patrick Achard - One of the best experts on this subject based on the ideXlab platform.
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ethylene regulates arabidopsis development via the modulation of della protein growth repressor function
The Plant Cell, 2003Co-Authors: Patrick Achard, Dominique Van Der Straeten, Willem Vriezen, Nicholas P HarberdAbstract:Phytohormones regulate plant development via a poorly understood Signal Response network. Here, we show that the phytohormone ethylene regulates plant development at least in part via alteration of the properties of DELLA protein nuclear growth repressors, a family of proteins first identified as gibberellin (GA) Signaling components. This conclusion is based on the following experimental observations. First, ethylene inhibited Arabidopsis root growth in a DELLA-dependent manner. Second, ethylene delayed the GA-induced disappearance of the DELLA protein repressor of ga1-3 from root cell nuclei via a constitutive triple Response-dependent Signaling pathway. Third, the ethylene-promoted "apical hook" structure of etiolated seedling hypocotyls was dependent on the relief of DELLA-mediated growth restraint. Ethylene, auxin, and GA Responses now can be attributed to effects on DELLA function, suggesting that DELLA plays a key integrative role in the phytohormone Signal Response network.
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ethylene regulates arabidopsis development via the modulation of della protein growth repressor function
The Plant Cell, 2003Co-Authors: Patrick Achard, Willem Vriezen, Dominique Van Der Straeten, Nicholas P HarberdAbstract:Phytohormones regulate plant development via a poorly understood Signal Response network. Here, we show that the phytohormone ethylene regulates plant development at least in part via alteration of the properties of DELLA protein nuclear growth repressors, a family of proteins first identified as gibberellin (GA) Signaling components. This conclusion is based on the following experimental observations. First, ethylene inhibited Arabidopsis root growth in a DELLA-dependent manner. Second, ethylene delayed the GA-induced disappearance of the DELLA protein repressor of ga1-3 from root cell nuclei via a constitutive triple Response-dependent Signaling pathway. Third, the ethylene-promoted “apical hook” structure of etiolated seedling hypocotyls was dependent on the relief of DELLA-mediated growth restraint. Ethylene, auxin, and GA Responses now can be attributed to effects on DELLA function, suggesting that DELLA plays a key integrative role in the phytohormone Signal Response network.
L. Ouerdane - One of the best experts on this subject based on the ideXlab platform.
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nonlinear Signal Response in electrospray mass spectrometry implications for quantitation of arsenobetaine using stable isotope labeling by liquid chromatography and electrospray orbitrap mass spectrometry
Analytical Chemistry, 2012Co-Authors: L. Ouerdane, J. Meija, S. Bakirdere, L. Yang, Z. MesterAbstract:Isotope amount ratio measurements by electrospray ionization mass spectrometry show large systematic biases. Moreover, the Signal ratio Response can vary nonlinearly with respect to the amount ratio depending on the concentration of the analyte or coeluting matrix components, among other things. Since isotope dilution relies inherently on the linearity of Response, accurate quantitation is then more difficult to achieve. In this study, we outline a method to eliminate the quantitation errors due to the effects of the nonlinear Signal Response. The proposed approach is a hybrid of the method of standard additions and isotope dilution allowing correction for nonlinear trend. As a proof of concept, determination of arsenobetaine content in fish tissue was performed using liquid chromatography coupled with a linear quadrupole ion trap (LTQ) Orbitrap mass spectrometer. The nonlinear isotope dilution method could, in principle, be applied to correct isotope ratio measurement biases in popular relative quantitation methods of biomolecules such as stable isotope labeling by amino acids in cell culture (SILAC), isotope-coded affinity tag (ICAT), or isobaric tags for relative and absolute quantification (iTRAQ).
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Nonlinear Signal Response in Electrospray Mass Spectrometry: Implications for Quantitation of Arsenobetaine Using Stable Isotope Labeling by Liquid Chromatography and Electrospray Orbitrap Mass Spectrometry
2012Co-Authors: L. Ouerdane, J. Meija, S. Bakirdere, L. Yang, Z. MesterAbstract:Isotope amount ratio measurements by electrospray ionization mass spectrometry show large systematic biases. Moreover, the Signal ratio Response can vary nonlinearly with respect to the amount ratio depending on the concentration of the analyte or coeluting matrix components, among other things. Since isotope dilution relies inherently on the linearity of Response, accurate quantitation is then more difficult to achieve. In this study, we outline a method to eliminate the quantitation errors due to the effects of the nonlinear Signal Response. The proposed approach is a hybrid of the method of standard additions and isotope dilution allowing correction for nonlinear trend. As a proof of concept, determination of arsenobetaine content in fish tissue was performed using liquid chromatography coupled with a linear quadrupole ion trap (LTQ) Orbitrap mass spectrometer. The nonlinear isotope dilution method could, in principle, be applied to correct isotope ratio measurement biases in popular relative quantitation methods of biomolecules such as stable isotope labeling by amino acids in cell culture (SILAC), isotope-coded affinity tag (ICAT), or isobaric tags for relative and absolute quantification (iTRAQ)
Eyal Buks - One of the best experts on this subject based on the ideXlab platform.
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quantum analysis of a linear dc squid mechanical displacement detector
Physical Review B, 2007Co-Authors: M P Blencowe, Eyal BuksAbstract:We provide a quantum analysis of a dc SQUID mechanical displacement detector within the subcritical Josephson current regime. A segment of the SQUID loop forms the mechanical resonator and motion of the latter is transduced inductively through changes in the flux threading the loop. Expressions are derived for the detector Signal Response and noise, which are used to evaluate the position and force detection sensitivity. We also investigate cooling of the mechanical resonator due to detector back reaction.
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quantum analysis of a linear dc squid mechanical displacement detector
Physical Review B, 2007Co-Authors: M P Blencowe, Eyal BuksAbstract:We provide a quantum analysis of a DC SQUID mechanical displacement detector within the sub-critical Josephson current regime. A segment of the SQUID loop forms the mechanical resonator and motion of the latter is transduced inductively through changes in the flux threading the loop. Expressions are derived for the detector Signal Response and noise, which are used to evaluate the position and force detection sensitivity. We also investigate cooling of the mechanical resonator due to back reaction noise from the detector.
