The Experts below are selected from a list of 55143 Experts worldwide ranked by ideXlab platform
Jiankang Shen - One of the best experts on this subject based on the ideXlab platform.
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gambogic acid induces egfr degradation and akt mtorc1 inhibition through ampk dependent lrig1 upregulation in cultured u87 glioma cells
Biochemical and Biophysical Research Communications, 2013Co-Authors: Xinyao He, Xiao Chen, Liuguan Bian, Weiguo Zhao, Jiankang ShenAbstract:Abstract Glioblastoma multiforme (GBM) is the most common malignant tumor in adults’ central nervous system (CNS). The development of novel anti-cancer agents for GBM is urgent. In the current study, we found that gambogic acid induced growth inhibition and apoptosis in cultured U87 glioma cells, which was associated with Akt/ mTORC1 (mTOR complex 1) Signaling in-activation. To restore Akt activation by introducing a constitutively active (CA) Akt attenuated gambogic acid-induced cytotoxicity against U87 cells. For Mechanism study, we found that gambogic acid induced LRIG1 (leucine-rich repeat and Ig-like domain-containing-1) upregulation, which was responsible for EGFR (epidermal growth factor receptor) degradation and its downstream Akt/mTORC1 inhibition. Further, we provided evidence to support that AMPK (AMP-activated protein kinase) activation mediated gambogic acid-induced LRIG1 upregulation, U87 cell apoptosis and growth inhibition, while AMPK inhibition by shRNA or compound C reduced gambogic acid-induced EGFR/Akt inhibition and cytotoxicity in U87 cells. We here proposed novel Signaling Mechanism mediating gambogic acid-induced cytotoxic effects in glioma cells.
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Gambogic acid induces EGFR degradation and Akt/mTORC1 inhibition through AMPK dependent-LRIG1 upregulation in cultured U87 glioma cells
Biochemical and Biophysical Research Communications, 2013Co-Authors: Xinyao He, Xiao Chen, Liuguan Bian, Weiguo Zhao, Jiankang ShenAbstract:Abstract Glioblastoma multiforme (GBM) is the most common malignant tumor in adults’ central nervous system (CNS). The development of novel anti-cancer agents for GBM is urgent. In the current study, we found that gambogic acid induced growth inhibition and apoptosis in cultured U87 glioma cells, which was associated with Akt/ mTORC1 (mTOR complex 1) Signaling in-activation. To restore Akt activation by introducing a constitutively active (CA) Akt attenuated gambogic acid-induced cytotoxicity against U87 cells. For Mechanism study, we found that gambogic acid induced LRIG1 (leucine-rich repeat and Ig-like domain-containing-1) upregulation, which was responsible for EGFR (epidermal growth factor receptor) degradation and its downstream Akt/mTORC1 inhibition. Further, we provided evidence to support that AMPK (AMP-activated protein kinase) activation mediated gambogic acid-induced LRIG1 upregulation, U87 cell apoptosis and growth inhibition, while AMPK inhibition by shRNA or compound C reduced gambogic acid-induced EGFR/Akt inhibition and cytotoxicity in U87 cells. We here proposed novel Signaling Mechanism mediating gambogic acid-induced cytotoxic effects in glioma cells.
Shikang Wu - One of the best experts on this subject based on the ideXlab platform.
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fluorescence turn on of coumarin derivatives by metal cations a new Signaling Mechanism based on c n isomerization
Organic Letters, 2007Co-Authors: Jiasheng Wu, Pengfei Wang, Xiaohong Zhang, Xiaoqing Zhuang, Fang Wang, Shikang WuAbstract:A new sensing Mechanism based on CN isomerization, which shows a very significant fluorescence enhancement to the metal cations in a simple and efficient way, is demonstrated. A coumarin derivative (L) containing a CN group was designed as an example for illustration. The free ligand L is almost nonfluorescent due to the isomerization of CN double bond in the excited state. However, the solution of ligand shows about a 200-fold increase of fluorescence quantum yield (about 30%) upon addition of Zn(ClO4)2.
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new fluorescent chemosensor based on exciplex Signaling Mechanism
Organic Letters, 2005Co-Authors: Jiasheng Wu, Jianhua Zhou, Pengfei Wang, Xiaohong Zhang, Shikang WuAbstract:A novel fluorescent chemosensor (compound 1) containing aminonaphthol, which selectively recognizes fluoride anion with high sensitivity, was synthesized. The fluorescence of compound 1 was quenched rapidly by fluoride ion, and a new peak at a longer wavelength emerged concurrently, which constituted the signature for fluoride detection. The Mechanism of exciplex formation was proposed for the interesting observation.
Xinyao He - One of the best experts on this subject based on the ideXlab platform.
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gambogic acid induces egfr degradation and akt mtorc1 inhibition through ampk dependent lrig1 upregulation in cultured u87 glioma cells
Biochemical and Biophysical Research Communications, 2013Co-Authors: Xinyao He, Xiao Chen, Liuguan Bian, Weiguo Zhao, Jiankang ShenAbstract:Abstract Glioblastoma multiforme (GBM) is the most common malignant tumor in adults’ central nervous system (CNS). The development of novel anti-cancer agents for GBM is urgent. In the current study, we found that gambogic acid induced growth inhibition and apoptosis in cultured U87 glioma cells, which was associated with Akt/ mTORC1 (mTOR complex 1) Signaling in-activation. To restore Akt activation by introducing a constitutively active (CA) Akt attenuated gambogic acid-induced cytotoxicity against U87 cells. For Mechanism study, we found that gambogic acid induced LRIG1 (leucine-rich repeat and Ig-like domain-containing-1) upregulation, which was responsible for EGFR (epidermal growth factor receptor) degradation and its downstream Akt/mTORC1 inhibition. Further, we provided evidence to support that AMPK (AMP-activated protein kinase) activation mediated gambogic acid-induced LRIG1 upregulation, U87 cell apoptosis and growth inhibition, while AMPK inhibition by shRNA or compound C reduced gambogic acid-induced EGFR/Akt inhibition and cytotoxicity in U87 cells. We here proposed novel Signaling Mechanism mediating gambogic acid-induced cytotoxic effects in glioma cells.
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Gambogic acid induces EGFR degradation and Akt/mTORC1 inhibition through AMPK dependent-LRIG1 upregulation in cultured U87 glioma cells
Biochemical and Biophysical Research Communications, 2013Co-Authors: Xinyao He, Xiao Chen, Liuguan Bian, Weiguo Zhao, Jiankang ShenAbstract:Abstract Glioblastoma multiforme (GBM) is the most common malignant tumor in adults’ central nervous system (CNS). The development of novel anti-cancer agents for GBM is urgent. In the current study, we found that gambogic acid induced growth inhibition and apoptosis in cultured U87 glioma cells, which was associated with Akt/ mTORC1 (mTOR complex 1) Signaling in-activation. To restore Akt activation by introducing a constitutively active (CA) Akt attenuated gambogic acid-induced cytotoxicity against U87 cells. For Mechanism study, we found that gambogic acid induced LRIG1 (leucine-rich repeat and Ig-like domain-containing-1) upregulation, which was responsible for EGFR (epidermal growth factor receptor) degradation and its downstream Akt/mTORC1 inhibition. Further, we provided evidence to support that AMPK (AMP-activated protein kinase) activation mediated gambogic acid-induced LRIG1 upregulation, U87 cell apoptosis and growth inhibition, while AMPK inhibition by shRNA or compound C reduced gambogic acid-induced EGFR/Akt inhibition and cytotoxicity in U87 cells. We here proposed novel Signaling Mechanism mediating gambogic acid-induced cytotoxic effects in glioma cells.
Jiasheng Wu - One of the best experts on this subject based on the ideXlab platform.
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fluorescence turn on of coumarin derivatives by metal cations a new Signaling Mechanism based on c n isomerization
Organic Letters, 2007Co-Authors: Jiasheng Wu, Pengfei Wang, Xiaohong Zhang, Xiaoqing Zhuang, Fang Wang, Shikang WuAbstract:A new sensing Mechanism based on CN isomerization, which shows a very significant fluorescence enhancement to the metal cations in a simple and efficient way, is demonstrated. A coumarin derivative (L) containing a CN group was designed as an example for illustration. The free ligand L is almost nonfluorescent due to the isomerization of CN double bond in the excited state. However, the solution of ligand shows about a 200-fold increase of fluorescence quantum yield (about 30%) upon addition of Zn(ClO4)2.
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new fluorescent chemosensor based on exciplex Signaling Mechanism
Organic Letters, 2005Co-Authors: Jiasheng Wu, Jianhua Zhou, Pengfei Wang, Xiaohong Zhang, Shikang WuAbstract:A novel fluorescent chemosensor (compound 1) containing aminonaphthol, which selectively recognizes fluoride anion with high sensitivity, was synthesized. The fluorescence of compound 1 was quenched rapidly by fluoride ion, and a new peak at a longer wavelength emerged concurrently, which constituted the signature for fluoride detection. The Mechanism of exciplex formation was proposed for the interesting observation.
He Tian - One of the best experts on this subject based on the ideXlab platform.
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a ratiometric fluorescent chemosensor for fluoride ions based on a proton transfer Signaling Mechanism
Journal of Materials Chemistry, 2005Co-Authors: He TianAbstract:We report an easy-to-prepare fluorescent chemosensor, 4-benzoylamido-N-butyl-1,8-naphthalimide, which is sensitive to fluoride ions. The sensor shows spectral shifts and intensity changes in the presence of fluoride, in a wavelength-ratiometric and -colorimetric manner, which can detect fluoride concentrations in a range of 20–100 µM at visible wavelengths. The striking colorless-to-yellow color change and blue-to-orange emission color change are thought to be due to the deprotonation of the 4-amido moiety of the naphthalimide fluorophore. The sensor compound shows great promise for the selective detection of fluoride in the presence of other halides because none of those are likely to deprotonate the amide.
