The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Hanzhong Wang - One of the best experts on this subject based on the ideXlab platform.
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important photosynthetic contribution of Silique wall to seed yield related traits in arabidopsis thaliana
Photosynthesis Research, 2018Co-Authors: Xiaoyi Zhu, Hanzhong Wang, Liang Zhang, Chen Kuang, Yan Guo, Chunqian Huang, Linbin Deng, Xingchao Sun, Gaomiao Zhan, Wei HuaAbstract:In plants, green non-foliar organs are able to perform photosynthesis just as leaves do, and the seed-enclosing pod acts as an essential photosynthetic organ in legume and Brassica species. To date, the contribution of pod photosynthesis to seed yield and related components still remains largely unexplored, and in Arabidopsis thaliana, the photosynthetic activity of the Silique (pod) is unknown. In this study, an Arabidopsis glk1/glk2 mutant defective in both leaf and Silique photosynthesis was used to create tissue-specific functional complementation lines. These lines were used to analyze the contribution of Silique wall photosynthesis to seed yield and related traits, and to permit the comparison of this contribution with that of leaf photosynthesis. Our results showed that, together with leaves, the photosynthetic assimilation of the Silique wall greatly contributed to total seed yield per plant. As for individual components of yield traits, leaf photosynthesis alone contributed to the seed number per Silique and Silique length, while Silique wall photosynthesis alone contributed to thousand-seed weight. In addition, enhancing the photosynthetic capacity of the Silique wall by overexpressing the photosynthesis-related RCA gene in this tissue resulted in significantly increased seed weight and oil content in the wild-type (WT) background. These results reveal that Silique wall photosynthesis plays an important role in seed-related traits, and that enhancing Silique photosynthesis in WT plants can further improve seed yield-related traits and oil production. This finding may have significant implications for improving the seed yield and oil production of oilseed crops and other species with pod-like organs.
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bnlate a cys2 his2 type zinc finger protein enhances Silique shattering resistance by negatively regulating lignin accumulation in the Silique walls of brassica napus
PLOS ONE, 2017Co-Authors: Zhangsheng Tao, Yi Huang, Lida Zhang, Xinfa Wang, Guihua Liu, Hanzhong WangAbstract:Silique shattering resistance is one of the most important agricultural traits in oil crop breeding. Seed shedding from Siliques prior to and during harvest causes devastating losses in oilseed yield. Lignin biosynthesis in the Silique walls is thought to affect Silique-shattering resistance in oil crops. Here, we identified and characterized B. napus LATE FLOWERING (BnLATE), which encodes a Cys2/His2-type zinc-finger protein. Heterologous expression of BnLATE under the double enhanced CaMV 35S promoter (D35S) in wild-type Arabidopsis plants resulted in a marked decrease in lignification in the replum, valve layer (carpel) and dehiscence zone. pBnLATE::GUS activity was strong in the yellowing Silique walls of transgenic lines. Furthermore, the expression pattern of BnLATE and the lignin content gradient in the Silique walls at 48 days after pollination (DAP) of 73290, a B. napus Silique shattering-resistant line, are similar to those in transgenic Arabidopsis lines expressing BnLATE. Transcriptome sequencing of the Silique walls revealed that genes encoding peroxidases, which polymerize monolignols and lignin in the phenylpropanoid pathway, were down-regulated at least two-fold change in the D35S::BnLATE transgenic lines. pBnLATE::BnLATE transgenic lines were further used to identify the function of BnLATE, and the results showed that lignification in the carpel and dehiscence zone of yellowing Silique also remarkably decreased compared with the wild-type control, the Silique shattering-resistance and expression pattern of peroxidase genes are very similar to results with D35S::BnLATE. These results suggest that BnLATE is a negative regulator of lignin biosynthesis in the yellowing Silique walls, and promotes Silique-shattering resistance in B. napus through restraining the polymerization of monolignols and lignin.
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BnLATE, a Cys2/His2-Type Zinc-Finger Protein, Enhances Silique Shattering Resistance by Negatively Regulating Lignin Accumulation in the Silique Walls of Brassica napus
2017Co-Authors: Zhangsheng Tao, Yi Huang, Lida Zhang, Xinfa Wang, Guihua Liu, Hanzhong WangAbstract:Silique shattering resistance is one of the most important agricultural traits in oil crop breeding. Seed shedding from Siliques prior to and during harvest causes devastating losses in oilseed yield. Lignin biosynthesis in the Silique walls is thought to affect Silique-shattering resistance in oil crops. Here, we identified and characterized B. napus LATE FLOWERING (BnLATE), which encodes a Cys2/His2-type zinc-finger protein. Heterologous expression of BnLATE under the double enhanced CaMV 35S promoter (D35S) in wild-type Arabidopsis plants resulted in a marked decrease in lignification in the replum, valve layer (carpel) and dehiscence zone. pBnLATE::GUS activity was strong in the yellowing Silique walls of transgenic lines. Furthermore, the expression pattern of BnLATE and the lignin content gradient in the Silique walls at 48 days after pollination (DAP) of 73290, a B. napus Silique shattering-resistant line, are similar to those in transgenic Arabidopsis lines expressing BnLATE. Transcriptome sequencing of the Silique walls revealed that genes encoding peroxidases, which polymerize monolignols and lignin in the phenylpropanoid pathway, were down-regulated at least two-fold change in the D35S::BnLATE transgenic lines. pBnLATE::BnLATE transgenic lines were further used to identify the function of BnLATE, and the results showed that lignification in the carpel and dehiscence zone of yellowing Silique also remarkably decreased compared with the wild-type control, the Silique shattering-resistance and expression pattern of peroxidase genes are very similar to results with D35S::BnLATE. These results suggest that BnLATE is a negative regulator of lignin biosynthesis in the yellowing Silique walls, and promotes Silique-shattering resistance in B. napus through restraining the polymerization of monolignols and lignin.
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natural variation in arf18 gene simultaneously affects seed weight and Silique length in polyploid rapeseed
Proceedings of the National Academy of Sciences of the United States of America, 2015Co-Authors: Jing Liu, Xinfa Wang, Liang Zhang, Linbin Deng, Xingchao Sun, Wei Hua, Hongli Yang, Hanzhong WangAbstract:Seed weight (SW), which is one of the three major factors influencing grain yield, has been widely accepted as a complex trait that is controlled by polygenes, particularly in polyploid crops. Brassica napus L., which is the second leading crop source for vegetable oil around the world, is a tetraploid (4×) species. In the present study, we identified a major quantitative trait locus (QTL) on chromosome A9 of rapeseed in which the genes for SW and Silique length (SL) were colocated. By fine mapping and association analysis, we uncovered a 165-bp deletion in the auxin-response factor 18 (ARF18) gene associated with increased SW and SL. ARF18 encodes an auxin-response factor and shows inhibitory activity on downstream auxin genes. This 55-aa deletion prevents ARF18 from forming homodimers, in turn resulting in the loss of binding activity. Furthermore, reciprocal crossing has shown that this QTL affects SW by maternal effects. Transcription analysis has shown that ARF18 regulates cell growth in the Silique wall by acting via an auxin-response pathway. Together, our results suggest that ARF18 regulates Silique wall development and determines SW via maternal regulation. In addition, our study reveals the first (to our knowledge) QTL in rapeseed and may provide insights into gene cloning involving polyploid crops.
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maternal control of seed oil content in brassica napus the role of Silique wall photosynthesis
Plant Journal, 2012Co-Authors: Wei Hua, Xinfa Wang, Guihua Liu, Gaomiao Zhan, Jing Liu, Hanzhong WangAbstract:Summary Seed oil content is an important agronomic trait in rapeseed. However, our understanding of the regulatory processes controlling oil accumulation is still limited. Using two rapeseed lines (zy036 and 51070) with contrasting oil content, we found that maternal genotype greatly affects seed oil content. Genetic and physiological evidence indicated that difference in the local and tissue-specific photosynthetic activity in the Silique wall (a maternal tissue) was responsible for the different seed oil contents. This effect was mimicked by in planta manipulation of Silique wall photosynthesis. Furthermore, the starch content and expression of the important lipid synthesis regulatory gene WRINKLED1 in developing seeds were linked with Silique wall photosynthetic activity. 454 pyrosequencing was performed to explore the possible molecular mechanism for the difference in Silique wall photosynthesis between zy036 and 51070. Interestingly, the results suggested that photosynthesis-related genes were over-represented in both total Silique wall expressed genes and genes that were differentially expressed between genotypes. A potential regulatory mechanism for elevated photosynthesis in the zy036 Silique wall is proposed on the basis of knowledge from Arabidopsis. Differentially expressed ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco)-related genes were used for further investigations. Oil content correlated closely with BnRBCS1A expression levels and Rubisco activities in the Silique wall, but not in the leaf. Taken together, our results highlight an important role of Silique wall photosynthesis in the regulation of seed oil content in terms of maternal effects.
Xinfa Wang - One of the best experts on this subject based on the ideXlab platform.
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bnlate a cys2 his2 type zinc finger protein enhances Silique shattering resistance by negatively regulating lignin accumulation in the Silique walls of brassica napus
PLOS ONE, 2017Co-Authors: Zhangsheng Tao, Yi Huang, Lida Zhang, Xinfa Wang, Guihua Liu, Hanzhong WangAbstract:Silique shattering resistance is one of the most important agricultural traits in oil crop breeding. Seed shedding from Siliques prior to and during harvest causes devastating losses in oilseed yield. Lignin biosynthesis in the Silique walls is thought to affect Silique-shattering resistance in oil crops. Here, we identified and characterized B. napus LATE FLOWERING (BnLATE), which encodes a Cys2/His2-type zinc-finger protein. Heterologous expression of BnLATE under the double enhanced CaMV 35S promoter (D35S) in wild-type Arabidopsis plants resulted in a marked decrease in lignification in the replum, valve layer (carpel) and dehiscence zone. pBnLATE::GUS activity was strong in the yellowing Silique walls of transgenic lines. Furthermore, the expression pattern of BnLATE and the lignin content gradient in the Silique walls at 48 days after pollination (DAP) of 73290, a B. napus Silique shattering-resistant line, are similar to those in transgenic Arabidopsis lines expressing BnLATE. Transcriptome sequencing of the Silique walls revealed that genes encoding peroxidases, which polymerize monolignols and lignin in the phenylpropanoid pathway, were down-regulated at least two-fold change in the D35S::BnLATE transgenic lines. pBnLATE::BnLATE transgenic lines were further used to identify the function of BnLATE, and the results showed that lignification in the carpel and dehiscence zone of yellowing Silique also remarkably decreased compared with the wild-type control, the Silique shattering-resistance and expression pattern of peroxidase genes are very similar to results with D35S::BnLATE. These results suggest that BnLATE is a negative regulator of lignin biosynthesis in the yellowing Silique walls, and promotes Silique-shattering resistance in B. napus through restraining the polymerization of monolignols and lignin.
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BnLATE, a Cys2/His2-Type Zinc-Finger Protein, Enhances Silique Shattering Resistance by Negatively Regulating Lignin Accumulation in the Silique Walls of Brassica napus
2017Co-Authors: Zhangsheng Tao, Yi Huang, Lida Zhang, Xinfa Wang, Guihua Liu, Hanzhong WangAbstract:Silique shattering resistance is one of the most important agricultural traits in oil crop breeding. Seed shedding from Siliques prior to and during harvest causes devastating losses in oilseed yield. Lignin biosynthesis in the Silique walls is thought to affect Silique-shattering resistance in oil crops. Here, we identified and characterized B. napus LATE FLOWERING (BnLATE), which encodes a Cys2/His2-type zinc-finger protein. Heterologous expression of BnLATE under the double enhanced CaMV 35S promoter (D35S) in wild-type Arabidopsis plants resulted in a marked decrease in lignification in the replum, valve layer (carpel) and dehiscence zone. pBnLATE::GUS activity was strong in the yellowing Silique walls of transgenic lines. Furthermore, the expression pattern of BnLATE and the lignin content gradient in the Silique walls at 48 days after pollination (DAP) of 73290, a B. napus Silique shattering-resistant line, are similar to those in transgenic Arabidopsis lines expressing BnLATE. Transcriptome sequencing of the Silique walls revealed that genes encoding peroxidases, which polymerize monolignols and lignin in the phenylpropanoid pathway, were down-regulated at least two-fold change in the D35S::BnLATE transgenic lines. pBnLATE::BnLATE transgenic lines were further used to identify the function of BnLATE, and the results showed that lignification in the carpel and dehiscence zone of yellowing Silique also remarkably decreased compared with the wild-type control, the Silique shattering-resistance and expression pattern of peroxidase genes are very similar to results with D35S::BnLATE. These results suggest that BnLATE is a negative regulator of lignin biosynthesis in the yellowing Silique walls, and promotes Silique-shattering resistance in B. napus through restraining the polymerization of monolignols and lignin.
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natural variation in arf18 gene simultaneously affects seed weight and Silique length in polyploid rapeseed
Proceedings of the National Academy of Sciences of the United States of America, 2015Co-Authors: Jing Liu, Xinfa Wang, Liang Zhang, Linbin Deng, Xingchao Sun, Wei Hua, Hongli Yang, Hanzhong WangAbstract:Seed weight (SW), which is one of the three major factors influencing grain yield, has been widely accepted as a complex trait that is controlled by polygenes, particularly in polyploid crops. Brassica napus L., which is the second leading crop source for vegetable oil around the world, is a tetraploid (4×) species. In the present study, we identified a major quantitative trait locus (QTL) on chromosome A9 of rapeseed in which the genes for SW and Silique length (SL) were colocated. By fine mapping and association analysis, we uncovered a 165-bp deletion in the auxin-response factor 18 (ARF18) gene associated with increased SW and SL. ARF18 encodes an auxin-response factor and shows inhibitory activity on downstream auxin genes. This 55-aa deletion prevents ARF18 from forming homodimers, in turn resulting in the loss of binding activity. Furthermore, reciprocal crossing has shown that this QTL affects SW by maternal effects. Transcription analysis has shown that ARF18 regulates cell growth in the Silique wall by acting via an auxin-response pathway. Together, our results suggest that ARF18 regulates Silique wall development and determines SW via maternal regulation. In addition, our study reveals the first (to our knowledge) QTL in rapeseed and may provide insights into gene cloning involving polyploid crops.
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maternal control of seed oil content in brassica napus the role of Silique wall photosynthesis
Plant Journal, 2012Co-Authors: Wei Hua, Xinfa Wang, Guihua Liu, Gaomiao Zhan, Jing Liu, Hanzhong WangAbstract:Summary Seed oil content is an important agronomic trait in rapeseed. However, our understanding of the regulatory processes controlling oil accumulation is still limited. Using two rapeseed lines (zy036 and 51070) with contrasting oil content, we found that maternal genotype greatly affects seed oil content. Genetic and physiological evidence indicated that difference in the local and tissue-specific photosynthetic activity in the Silique wall (a maternal tissue) was responsible for the different seed oil contents. This effect was mimicked by in planta manipulation of Silique wall photosynthesis. Furthermore, the starch content and expression of the important lipid synthesis regulatory gene WRINKLED1 in developing seeds were linked with Silique wall photosynthetic activity. 454 pyrosequencing was performed to explore the possible molecular mechanism for the difference in Silique wall photosynthesis between zy036 and 51070. Interestingly, the results suggested that photosynthesis-related genes were over-represented in both total Silique wall expressed genes and genes that were differentially expressed between genotypes. A potential regulatory mechanism for elevated photosynthesis in the zy036 Silique wall is proposed on the basis of knowledge from Arabidopsis. Differentially expressed ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco)-related genes were used for further investigations. Oil content correlated closely with BnRBCS1A expression levels and Rubisco activities in the Silique wall, but not in the leaf. Taken together, our results highlight an important role of Silique wall photosynthesis in the regulation of seed oil content in terms of maternal effects.
Hao Wang - One of the best experts on this subject based on the ideXlab platform.
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genome wide identification of Silique related traits based on high density genetic linkage map in brassica napus
Molecular Breeding, 2019Co-Authors: Weiguo Zhao, Hao Wang, Xiaodong Wang, Hongbo Chao, Lina Zhang, Jun Xiang, Libin ZhangAbstract:The yield of rapeseed is directly and indirectly influenced by Silique-related traits, the genetic dissection, and improvement of the Silique-related traits are thus the most important research project in rapeseed. Seven Silique-related traits, including seed number per Silique (SPS), Silique length (SL), Silique width (SW), Silique thickness (ST), Silique volume (SV), Silique density (SD), and thousand seed weight (TSW) were further analyzed through quantitative trait locus (QTL) mapping based on a high-density genetic linkage map in KN population. A total of 273 identified QTLs were integrated into 230 consensus QTLs, in which 84.78% of consensus QTLs were considered to be environment-specific QTLs and 15.22% of consensus QTLs were considered to be environmentally stable expression QTLs. Two major QTLs, including cqSW.C6-5 for SW and cqSPS.C6-3 for SPS were identified on C06. According to QTLs integrated from different Silique traits, 48 unique QTLs were identified with pleiotropy that involved in 2–5 Silique-related traits. In addition, 185 epistatic locus pairs were identified with the PV ranging from 0.96 to 15.98%. QTL comparison were made between the KN population and other mapping populations, a total of 164 QTLs for Silique-related traits (48 for SPS, 31 for SL, 7 for SD, and 78 for TSW) from nine previously reported researches were aligned to the Brassica napus reference genome, in which 23 QTLs were considered as new QTLs (8 for SD, 4 for SL, 7 for SPS, 4 for TSW). More importantly, cqSPS.C6-3 for SPS might be a novel major QTL. Furthermore, 82 candidate genes in B. napus corresponding to 48 candidate genes in Arabidopsis thaliana were identified, which were involved in transcription factors, enzymes, protein structure units, phytohormone response factors, and transporters, etc. These findings not only provided more comprehensive insights into the genetic basis for Silique-related traits but also new cues for improving Silique and increasing seed yield in B. napus.
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Genome-Wide Identification of the LAC Gene Family and Its Expression Analysis Under Stress in Brassica napus
MDPI AG, 2019Co-Authors: Xiaoke Ping, Tengyue Wang, Na Lin, Hongju Jian, Hao WangAbstract:Lignin is an important biological polymer in plants that is necessary for plant secondary cell wall ontogenesis. The laccase (LAC) gene family catalyzes lignification and has been suggested to play a vital role in the plant kingdom. In this study, we identified 45 LAC genes from the Brassica napus genome (BnLACs), 25 LAC genes from the Brassica rapa genome (BrLACs) and 8 LAC genes from the Brassica oleracea genome (BoLACs). These LAC genes could be divided into five groups in a cladogram and members in same group had similar structures and conserved motifs. All BnLACs contained hormone- and stress- related elements determined by cis-element analysis. The expression of BnLACs was relatively higher in the root, seed coat and stem than in other tissues. Furthermore, BnLAC4 and its predicted downstream genes showed earlier expression in the Silique pericarps of short Silique lines than long Silique lines. Three miRNAs (miR397a, miR397b and miR6034) target 11 BnLACs were also predicted. The expression changes of BnLACs under series of stresses were further investigated by RNA sequencing (RNA-seq) and quantitative real-time polymerase chain reaction (qRT-PCR). The study will give a deeper understanding of the LAC gene family evolution and functions in B. napus.
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quantitative trait loci analysis and genome wide comparison for Silique related traits in brassica napus
BMC Plant Biology, 2016Co-Authors: Xiaodong Wang, Li Chen, Jianhua Tian, Hao Wang, Aina Wang, Xiaoping Zhao, Hongbo Chao, Yajun Zhao, Weiguo ZhaoAbstract:Yield of rapeseed is determined by three components: Silique number, seed number per Silique and thousand seed weight. Seed number per Silique and thousand seed weight are influenced by Silique length, seed density, Silique breadth, Silique thickness and Silique volume. Some QTLs for Silique traits have been reported in B. napus, however, no studies have focused on the six agronomic traits (seed number per Silique, Silique length, Silique breadth, Silique thickness, seed density and Silique volume) simultaneously, and the genetic determinism of such complex traits have not been fully elucidated. In this study, the six Silique traits were evaluated using 348 lines of a doubled haploid population, the KN population. The results showed that 2, 4, 1, 1 and 2 QTLs explaining > 10 % of phenotypic variation were obtained for Silique length, Silique breadth, Silique thickness, seed number per Silique and Silique volume, respectively. Notably, three major effect QTLs (cqSB-C6-1, cqSB-C6-2 and cqSV-C6-3) were identified in at least three environments, and 17 unique QTLs controlling at least two traits were obtained. A high-density consensus map containing 1225 markers was constructed for QTL comparison by combining the KN map with other five published maps. The comparative results revealed that 14, 13 and 11 QTLs for Silique breadth, Silique thickness and Silique volume might be the potential new QTLs because few QTLs for these traits were reported in B. napus. In addition, potential new QTLs for Silique length (11), seed number per Silique (6) and seed density (5) were also identified. Twenty-five candidate genes underlying 27 QTLs for Silique related traits were obtained. This study constructed QTL analysis in B. napus, and obtained 60 consensus QTLs for six Silique related traits. The potential new QTLs will enhance our understanding of the genetic control of Silique traits, and the stable QTLs provided the targets for improving seed yield in future. These findings provided comprehensive insights into the genetic network affecting Silique traits at QTL level in B. napus.
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influence of leaf and Silique photosynthesis on seeds yield and seeds oil quality of oilseed rape brassica napus l
European Journal of Agronomy, 2016Co-Authors: Chunli Wang, Jiangbo Hai, Jianli Yang, Jianhua Tian, Wenjie Chen, Ting Chen, Hongbo Luo, Hao WangAbstract:With the purpose of enhancing oil production, the present work was carried out to elucidate relationships between photosynthesis of leaves, Siliques and seeds yield and seeds oil accumulation of oilseed rape. Field trials, in which two repeated experiments was carried out during 2012–2014 growth season, a rape hybrids the “Qin You No.7” (Brassica napus L.) variety was taken into account. The results showed that, on rape plant the photosynthetic capacity and chlorophyll a, b, carotenoid contents of leaf were significantly higher than that of Silique shell. Oil content of 94.7% was achieved in young seeds (sampled at 25th day after flowering ending stage of the tested rape plant) versus to mature seeds, saturated fatty acids percent was higher whereas oleic acid percent was lower of oil extracted from young seeds. During flowering period of the rape plants tested, area and dry weight of leaves attained maximum, treatments of removing leaves induced reduction in seeds number per Silique, Siliques number, seeds yield per plant and seeds oil content, these indexes were respectively decreased by 73.6%, 43.4%, 83.4% and 10.5% in maximum, and seeds oil composition was not significantly influenced; during seeds growing period of the tested plants, surface area and dry weight of Siliques attained maximum, under shading Siliques treatment, the 1000-seed weight, seeds yield per plant and seeds oil content were respectively reduced by 57.5%, 61.4% and 44.7% in maximum, and seeds oil oleic acid (C18:1) and linolenic acid (C18:3) percent was decreased, linolic acid (C18:2) and erucidic acid (C22:1) percent was increased. So for oilseed rape plant during flowering period, surface area and photosynthesis of leaves dramatically influenced Siliques number, seeds number and seeds yield; while in seeds growth period, surface area and photosynthesis of Siliques greatly influenced 1000-seed yield, seeds yield, seeds oil content and oil composition; oil accumulation in rape seeds initiated early since seeds commencing growth, seed mature degree influenced oil composition of seeds.
Jinxiong Shen - One of the best experts on this subject based on the ideXlab platform.
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increased seed number per Silique in brassica juncea by deleting cis regulatory region affecting bjclv1 expression in carpel margin meristem
Plant Biotechnology Journal, 2021Co-Authors: Gang Wang, Xiangxiang Zhang, Jing Wen, Wei Huang, Lun Zhao, Jinxiong ShenAbstract:Mustard yield per plant is severely restricted by the seed number per Silique. The seed number per Silique in the Brassica juncea trilocular mutant J163-4 is significantly greater than that in normal bilocular plants. However, how the trilocular Silique of J163-4 is formed remains unclear. Here, we studied the gene structure and function of mc2 in B. juncea and Arabidopsis using comparative morphology and molecular genetic experiments. We found that mc2 is a CLV1 ortholog, BjA7.CLV1. The deletion of cis-regulatory region in mc2 promoter, which affects Mc2 expression in carpel margin meristem (CMM), led to trilocular Silique formation. The BjCLV1 sequence with its complete promoter containing the cis-regulatory region can restore the Bjclv1 and clv1 mutant phenotypes in B. juncea and Arabidopsis, respectively. Additionally, this cis-regulatory region had a collinear segment in the promoter of CLV1 homologous gene in most Brassicaceae species. Our results are consistent with the report that BjCLV1 represents a conserved pleiotropic role in shoot meristem and CMM development, which contains a cis-regulatory sequence specifically expressed BjCLV1 in CMM in its promoter, and this cis-regulatory region is conserved in Brassicaceae species. These results offer a reliable approach for fine-tuning the traits of seed yield in Brassicaceae crops.
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fine mapping of a Silique length and seed weight related gene in brassica napus
Theoretical and Applied Genetics, 2019Co-Authors: Wenhao Shen, Jing Wen, Pei Qin, Mengjiao Yan, Jinxiong ShenAbstract:Using microarray analysis combined with map-based cloning, a major locus positively regulating SL and SW was mapped to a 98.47 kb interval on A09 in rapeseed. In rapeseed, seed yield is closely associated with Silique-related traits such as Silique length (SL) and seed weight (SW). Previously identified quantitative trait loci (QTLs) revealed that SL and SW are complex traits and many QTLs overlap. However, the genetic characterization of the association between SL and SW is poorly understood. In the present study, a BC3F3 near isogenic line developed from a short Silique plant and the long Silique cultivar ‘ZS11’ was analyzed to identify the locus related to SL. Map-based cloning indicated that a major locus acting as a single Mendelian factor was mapped to a 98.47 kb region on chromosome A09. BLAST analysis and DNA sequencing showed SNP variations and a fragment replacement in the upstream region of the candidate gene BnaA09g55530D may alter gene expression and influence SL. The results showed that this SL locus may also positively affect SW as well as in the 186 rapeseed accessions identified by the associated markers. Therefore, selecting plants with appropriate SL and developing functional markers for the associated gene could play important roles in the molecular breeding of high-yield rapeseed varieties.
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identification of mirnas that regulate Silique development in brassica napus
Plant Science, 2018Co-Authors: Li Chen, Lei Chen, Xiangxiang Zhang, Tingting Liu, Sailun Niu, Jing Wen, Jinxiong ShenAbstract:MicroRNAs (miRNAs) are a class of non-coding small RNAs (sRNAs) that play crucial regulatory roles in various developmental processes. Silique length indirectly influences seed yield in rapeseed (Brassica napus); however, the molecular roles of miRNAs in Silique length are largely unknown. Here, backcross progenies of rapeseed with long Siliques (LS) and short Siliques (SS) were used to elucidate these roles. Four small RNA libraries from Siliques in an early stage of development were sequenced, and a total of 814 non-redundant miRNA precursors were identified, representing 65 known and 394 novel miRNAs. Expression analyses revealed that 17 miRNAs were differentially expressed in LS and SS lines. Furthermore, through degradome sequencing, we identified 522 cleavage events. Correlation analysis of the differentially expressed miRNAs and their targets suggested that miR159 and miR319 represses cell proliferation and miR160 regulates auxin signal transduction to control Silique length. Additionally, the upregulation of miR2111, miR399, miR827, and miR408 reflected restricted Silique development due to inorganic phosphate/copper deficiency. More significantly, high expression of miR160 in rapeseed may repress auxin response factors and result in increased Silique length, illustrating that Silique length might be regulated via an auxin-response pathway.
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interpreting the genetic basis of Silique traits in brassica napus using a joint qtl network
Plant Breeding, 2014Co-Authors: Long Mao, Jinxiong Shen, Chengming Sun, Jing WenAbstract:The objectives of this study were to decipher the genetic determinants of Silique length (SL), number of seeds per Silique (SN), thousand seed weight (TSW) and to reveal interactions between trait loci. A double haploid population of 181 lines derived from a cross between two Brassica napus genotypes exhibiting contrasting Silique traits was evaluated for Silique traits under three environmental conditions. Correlation analysis revealed a significant positive correlation between SL and TSW, a weak positive correlation between SL and SN and a negative correlation between SN and TSW. Quantitative trait loci (QTL) mapping detected 14 additive QTL and 7 QTL pairs with epistatic interactions. Three additive QTL located on LG A9, A1 and A9 had the greatest effect, explaining 51.49%, 23.54% and 27.15% of the phenotypic variance for SL, SN and TSW, respectively. Additionally, the additive × environment and epistatic × environment interaction effects were negligible, indicating the stability of the genetic effects for these QTL. The results will promote a more comprehensive description of the genetic basis of these Silique traits in B. napus.
Zhangsheng Tao - One of the best experts on this subject based on the ideXlab platform.
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bnlate a cys2 his2 type zinc finger protein enhances Silique shattering resistance by negatively regulating lignin accumulation in the Silique walls of brassica napus
PLOS ONE, 2017Co-Authors: Zhangsheng Tao, Yi Huang, Lida Zhang, Xinfa Wang, Guihua Liu, Hanzhong WangAbstract:Silique shattering resistance is one of the most important agricultural traits in oil crop breeding. Seed shedding from Siliques prior to and during harvest causes devastating losses in oilseed yield. Lignin biosynthesis in the Silique walls is thought to affect Silique-shattering resistance in oil crops. Here, we identified and characterized B. napus LATE FLOWERING (BnLATE), which encodes a Cys2/His2-type zinc-finger protein. Heterologous expression of BnLATE under the double enhanced CaMV 35S promoter (D35S) in wild-type Arabidopsis plants resulted in a marked decrease in lignification in the replum, valve layer (carpel) and dehiscence zone. pBnLATE::GUS activity was strong in the yellowing Silique walls of transgenic lines. Furthermore, the expression pattern of BnLATE and the lignin content gradient in the Silique walls at 48 days after pollination (DAP) of 73290, a B. napus Silique shattering-resistant line, are similar to those in transgenic Arabidopsis lines expressing BnLATE. Transcriptome sequencing of the Silique walls revealed that genes encoding peroxidases, which polymerize monolignols and lignin in the phenylpropanoid pathway, were down-regulated at least two-fold change in the D35S::BnLATE transgenic lines. pBnLATE::BnLATE transgenic lines were further used to identify the function of BnLATE, and the results showed that lignification in the carpel and dehiscence zone of yellowing Silique also remarkably decreased compared with the wild-type control, the Silique shattering-resistance and expression pattern of peroxidase genes are very similar to results with D35S::BnLATE. These results suggest that BnLATE is a negative regulator of lignin biosynthesis in the yellowing Silique walls, and promotes Silique-shattering resistance in B. napus through restraining the polymerization of monolignols and lignin.
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BnLATE, a Cys2/His2-Type Zinc-Finger Protein, Enhances Silique Shattering Resistance by Negatively Regulating Lignin Accumulation in the Silique Walls of Brassica napus
2017Co-Authors: Zhangsheng Tao, Yi Huang, Lida Zhang, Xinfa Wang, Guihua Liu, Hanzhong WangAbstract:Silique shattering resistance is one of the most important agricultural traits in oil crop breeding. Seed shedding from Siliques prior to and during harvest causes devastating losses in oilseed yield. Lignin biosynthesis in the Silique walls is thought to affect Silique-shattering resistance in oil crops. Here, we identified and characterized B. napus LATE FLOWERING (BnLATE), which encodes a Cys2/His2-type zinc-finger protein. Heterologous expression of BnLATE under the double enhanced CaMV 35S promoter (D35S) in wild-type Arabidopsis plants resulted in a marked decrease in lignification in the replum, valve layer (carpel) and dehiscence zone. pBnLATE::GUS activity was strong in the yellowing Silique walls of transgenic lines. Furthermore, the expression pattern of BnLATE and the lignin content gradient in the Silique walls at 48 days after pollination (DAP) of 73290, a B. napus Silique shattering-resistant line, are similar to those in transgenic Arabidopsis lines expressing BnLATE. Transcriptome sequencing of the Silique walls revealed that genes encoding peroxidases, which polymerize monolignols and lignin in the phenylpropanoid pathway, were down-regulated at least two-fold change in the D35S::BnLATE transgenic lines. pBnLATE::BnLATE transgenic lines were further used to identify the function of BnLATE, and the results showed that lignification in the carpel and dehiscence zone of yellowing Silique also remarkably decreased compared with the wild-type control, the Silique shattering-resistance and expression pattern of peroxidase genes are very similar to results with D35S::BnLATE. These results suggest that BnLATE is a negative regulator of lignin biosynthesis in the yellowing Silique walls, and promotes Silique-shattering resistance in B. napus through restraining the polymerization of monolignols and lignin.
