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Erminio Trevisi - One of the best experts on this subject based on the ideXlab platform.
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short communication inflammation migration and cell cell interaction related gene network expression in leukocytes is enhanced in Simmental compared with holstein dairy cows after calving
Journal of Dairy Science, 2020Co-Authors: V. Lopreiato, Andrea Minuti, Domenico Britti, Valeria Maria Morittu, Juan J. Loor, F Picciolicappelli, Erminio TrevisiAbstract:The aim of this study was to investigate changes in the abundance of genes involved in leukocyte function between cows highly specialized for milk production (Holstein, n = 12) and cows selected for meat and milk (Simmental, n = 13). Blood was collected on d 3 after calving in PAXgene tubes (Preanalytix, Hombrechtikon, Switzerland) to measure mRNA abundance of 33 genes. Normalized gene abundance data were subjected to MIXED model ANOVA using SAS (SAS Institute Inc. Cary, NC). Simmental cows had greater transcript abundance of proinflammatory cytokines and receptor genes (IL1B, TNF, IL1R, TNFRSF1A), cell migration- and adhesion-related genes (CX3CR1, ITGB2, CD44, LGALS8), and the antimicrobial IDO1 gene. In contrast, compared with Holstein cows, Simmental cows had lower abundance of the toll-like receptor (TLR) recognition-related gene TLR2, the antimicrobial-related gene LTF, and S100A8, which is involved in cell maturation, regulation of inflammatory processes, and immune response. These results revealed that breed plays an important role in the modulation of genes involved in immune adaptation and inflammatory response, and the immune system of Simmental cows could potentially have a more acute response in early lactation. In turn, this might be beneficial for mounting a more efficient response after calving and allow for a smoother homeorhetic adaptation to lactation.
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Immunometabolic status and productive performance differences between periparturient Simmental and Holstein dairy cows in response to pegbovigrastim
Journal of dairy science, 2019Co-Authors: V. Lopreiato, Andrea Minuti, Francesca Trimboli, Domenico Britti, Valeria Maria Morittu, F. Piccioli Cappelli, Juan J. Loor, Erminio TrevisiAbstract:In the present study, we aimed to investigate the side effects of pegbovigrastim, injected approximately 7 d before parturition and on the day of calving, on a panel of plasma biomarkers to evaluate energy, inflammatory, oxidative, and liver function status. We also addressed treatment responses in different breeds during the transition period. Holstein and Simmental cows were randomly assigned into 2 groups based on expected calving date and according to parity: the treated group (PEG; 14 Holstein and 12 Simmental cows) received pegylated recombinant bovine granulocyte colony stimulating factor (pegbovigrastim, Imrestor; Elanco Animal Health, Greenfield, IN), and the control group (CTR; 14 Holstein and 14 Simmental cows) received saline solution. The PEG or CTR treatments were administered via subcutaneous injection in the scapular region at approximately 7 d (mean 7.80 ± 5.50 d) before expected parturition and within 24 h after calving. Blood samples were collected at -21, -7 (before injection), 1, 3, and 28 d relative to calving. Milk production was recorded at 7, 15, 21, 30, and 42 d. A mixed model with repeated measures was fitted to the normalized data using Proc MIXED of SAS (SAS Institute Inc., Cary, NC). Simmental PEG cows showed higher plasma protein concentrations at 1 and 3 d after calving compared with Simmental CTR and Holstein PEG cows, whereas no differences were detected between Holstein PEG and CTR cows. Albumin was greater at 1 d in Simmental PEG compared with Simmental CTR cows. In contrast, γ-glutamyl transferase was higher overall (across breed) in PEG than in CTR. The PEG group had higher values throughout the postcalving period compared with CTR. Cows treated with pegbovigrastim had also higher alkaline phosphatase (ALP) activity at 1 and 3 d after calving. The Holstein PEG group had higher ALP activity at 3 d compared with the Holstein CTR and Simmental PEG groups, and higher ALP at 1 d compared with the Simmental CTR group. The PEG group had higher levels of IL-6 at 3 and 28 d but higher IL-1β only at 28 d after calving compared with the CTR group. Overall, Holstein cows were characterized by a greater response in the production of inflammation biomarkers (cytokines, haptoglobin, and ceruloplasmin). In addition, PEG cows had higher values of zinc at 1 and 3 d after calving compared with CTR cows. The response observed in plasma biomarkers for energy metabolism and liver functionality after pegbovigrastim treatment in Simmental and Holstein cows was not different from that in control cows. However, our data shed light on the different metabolic adaptations during the transition period between Simmental and Holstein cows, characterized by different energy, inflammatory, and oxidative pattern responses. For the first time, we have highlighted the effect of pegbovigrastim in maintaining stable cytokine levels during the first month after parturition, reflecting greater regulation of neutrophil recruitment, trafficking, and maturation during the inflammatory response. These results provide evidence of the immunomodulatory action of pegbovigrastim around parturition, when dairy cows are highly immunosuppressed. At the same time, these data demonstrate that increasing release of cytokines after parturition is not linked to exacerbation of a systemic inflammation evaluated based on haptoglobin and ceruloplasmin levels.
Silvester Žgur - One of the best experts on this subject based on the ideXlab platform.
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Carcass and meat quality of young Cika and Simmental bulls finished under similar conditions
Animal Production Science, 2018Co-Authors: M. Simčič, Marko Čepon, Silvester ŽgurAbstract:Twenty indigenous Cika and 20 young Simmental bulls (slaughtered at 560–718 days) were finished on two diets (extensive vs semi-intensive). When fed similar rations, Cika bulls at a ‘suitable’ finished level, had a significantly higher dressing percentage (+2.5%), more total fat (+13.7%), a lower percentage of tendons (–22.7%) and bones (–7.7%), a higher lean meat to bones ratio (+8.1%) and a greater percentage of intramuscular fat (+45.9%) than the Simmental bulls. Their meat also had a significantly lower pH (–4.0%), higher redness (+13.74%), yellowness (+15.0%) and drip loss (after 2 days, +28.3%) and a more intense flavour (+6.1%). Cika fat contained higher proportions of saturated fatty acids (+6.86%) and less polyunsaturated fatty acids (–30.8%) than Simmental fat. However, it appears that the main difference between these two breeds were the slower growth rate of the Cika and their lower weight but greater age when a finished state was reached relative to the Simmental.
M. Simčič - One of the best experts on this subject based on the ideXlab platform.
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Carcass and meat quality of young Cika and Simmental bulls finished under similar conditions
Animal Production Science, 2018Co-Authors: M. Simčič, Marko Čepon, Silvester ŽgurAbstract:Twenty indigenous Cika and 20 young Simmental bulls (slaughtered at 560–718 days) were finished on two diets (extensive vs semi-intensive). When fed similar rations, Cika bulls at a ‘suitable’ finished level, had a significantly higher dressing percentage (+2.5%), more total fat (+13.7%), a lower percentage of tendons (–22.7%) and bones (–7.7%), a higher lean meat to bones ratio (+8.1%) and a greater percentage of intramuscular fat (+45.9%) than the Simmental bulls. Their meat also had a significantly lower pH (–4.0%), higher redness (+13.74%), yellowness (+15.0%) and drip loss (after 2 days, +28.3%) and a more intense flavour (+6.1%). Cika fat contained higher proportions of saturated fatty acids (+6.86%) and less polyunsaturated fatty acids (–30.8%) than Simmental fat. However, it appears that the main difference between these two breeds were the slower growth rate of the Cika and their lower weight but greater age when a finished state was reached relative to the Simmental.
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The effect of feeding technology on the haematological parameters of young Cika and Simmental bulls - short communication.
Veterinarski Arhiv, 2015Co-Authors: M. Klinkon, D. Kompan, M. SimčičAbstract:Cika cattle are an indigenous breed in Slovenia, while the Simmental breed is a cosmopolitan breed. The fattening trial was prepared to investigate whether Cika bulls are suitable for beef production, and the Simmental bulls were kept for comparison. The aim of this study was to investigate if different feeding technologies affect the haematological parameters of Cika and Simmental bulls. Blood samples of 17 Cika and 19 Simmental young bulls were taken in tubes with EDTA. The number of erythrocytes (RBC), leucocytes (WBC) and platelets (PLT), and the values of haemoglobin (Hb), haematocrit (PCV), the mean corpuscular haemoglobin (MCH), the mean corpuscular haemoglobin concentration (MCHC) and mean corpuscular volume (MCV) were measured with a haematological analyser ABC Vet. Differential white blood cells counts were also prepared. Data were analysed by the GLM procedure from the statistical package SAS/STAT regarding the breed, feeding technology and their interaction as fi xed effects. In the blood of Cika bulls there was a signifi cantly higher number of leucocytes as well as haemoglobin, haematocrit, MCV and MCH values than in the blood of Simmental bulls, while the number of platelets was higher in the blood of Simmental bulls. Different feeding technologies signifi cantly affected the erythrocyte count, and MCV and MCH values, where pasture reared bulls had lower erythrocyte and higher MCV and MCH values compared to stable fattened bulls. In the differential white blood cell count of Cika bulls there was a signifi cantly higher percentage of segmented neutrophils and a lower percentage of lymphocytes compared to Simmental bulls.
V. Lopreiato - One of the best experts on this subject based on the ideXlab platform.
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short communication inflammation migration and cell cell interaction related gene network expression in leukocytes is enhanced in Simmental compared with holstein dairy cows after calving
Journal of Dairy Science, 2020Co-Authors: V. Lopreiato, Andrea Minuti, Domenico Britti, Valeria Maria Morittu, Juan J. Loor, F Picciolicappelli, Erminio TrevisiAbstract:The aim of this study was to investigate changes in the abundance of genes involved in leukocyte function between cows highly specialized for milk production (Holstein, n = 12) and cows selected for meat and milk (Simmental, n = 13). Blood was collected on d 3 after calving in PAXgene tubes (Preanalytix, Hombrechtikon, Switzerland) to measure mRNA abundance of 33 genes. Normalized gene abundance data were subjected to MIXED model ANOVA using SAS (SAS Institute Inc. Cary, NC). Simmental cows had greater transcript abundance of proinflammatory cytokines and receptor genes (IL1B, TNF, IL1R, TNFRSF1A), cell migration- and adhesion-related genes (CX3CR1, ITGB2, CD44, LGALS8), and the antimicrobial IDO1 gene. In contrast, compared with Holstein cows, Simmental cows had lower abundance of the toll-like receptor (TLR) recognition-related gene TLR2, the antimicrobial-related gene LTF, and S100A8, which is involved in cell maturation, regulation of inflammatory processes, and immune response. These results revealed that breed plays an important role in the modulation of genes involved in immune adaptation and inflammatory response, and the immune system of Simmental cows could potentially have a more acute response in early lactation. In turn, this might be beneficial for mounting a more efficient response after calving and allow for a smoother homeorhetic adaptation to lactation.
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Immunometabolic status and productive performance differences between periparturient Simmental and Holstein dairy cows in response to pegbovigrastim
Journal of dairy science, 2019Co-Authors: V. Lopreiato, Andrea Minuti, Francesca Trimboli, Domenico Britti, Valeria Maria Morittu, F. Piccioli Cappelli, Juan J. Loor, Erminio TrevisiAbstract:In the present study, we aimed to investigate the side effects of pegbovigrastim, injected approximately 7 d before parturition and on the day of calving, on a panel of plasma biomarkers to evaluate energy, inflammatory, oxidative, and liver function status. We also addressed treatment responses in different breeds during the transition period. Holstein and Simmental cows were randomly assigned into 2 groups based on expected calving date and according to parity: the treated group (PEG; 14 Holstein and 12 Simmental cows) received pegylated recombinant bovine granulocyte colony stimulating factor (pegbovigrastim, Imrestor; Elanco Animal Health, Greenfield, IN), and the control group (CTR; 14 Holstein and 14 Simmental cows) received saline solution. The PEG or CTR treatments were administered via subcutaneous injection in the scapular region at approximately 7 d (mean 7.80 ± 5.50 d) before expected parturition and within 24 h after calving. Blood samples were collected at -21, -7 (before injection), 1, 3, and 28 d relative to calving. Milk production was recorded at 7, 15, 21, 30, and 42 d. A mixed model with repeated measures was fitted to the normalized data using Proc MIXED of SAS (SAS Institute Inc., Cary, NC). Simmental PEG cows showed higher plasma protein concentrations at 1 and 3 d after calving compared with Simmental CTR and Holstein PEG cows, whereas no differences were detected between Holstein PEG and CTR cows. Albumin was greater at 1 d in Simmental PEG compared with Simmental CTR cows. In contrast, γ-glutamyl transferase was higher overall (across breed) in PEG than in CTR. The PEG group had higher values throughout the postcalving period compared with CTR. Cows treated with pegbovigrastim had also higher alkaline phosphatase (ALP) activity at 1 and 3 d after calving. The Holstein PEG group had higher ALP activity at 3 d compared with the Holstein CTR and Simmental PEG groups, and higher ALP at 1 d compared with the Simmental CTR group. The PEG group had higher levels of IL-6 at 3 and 28 d but higher IL-1β only at 28 d after calving compared with the CTR group. Overall, Holstein cows were characterized by a greater response in the production of inflammation biomarkers (cytokines, haptoglobin, and ceruloplasmin). In addition, PEG cows had higher values of zinc at 1 and 3 d after calving compared with CTR cows. The response observed in plasma biomarkers for energy metabolism and liver functionality after pegbovigrastim treatment in Simmental and Holstein cows was not different from that in control cows. However, our data shed light on the different metabolic adaptations during the transition period between Simmental and Holstein cows, characterized by different energy, inflammatory, and oxidative pattern responses. For the first time, we have highlighted the effect of pegbovigrastim in maintaining stable cytokine levels during the first month after parturition, reflecting greater regulation of neutrophil recruitment, trafficking, and maturation during the inflammatory response. These results provide evidence of the immunomodulatory action of pegbovigrastim around parturition, when dairy cows are highly immunosuppressed. At the same time, these data demonstrate that increasing release of cytokines after parturition is not linked to exacerbation of a systemic inflammation evaluated based on haptoglobin and ceruloplasmin levels.
G P Gengelbach - One of the best experts on this subject based on the ideXlab platform.
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differences in copper status and copper metabolism among angus Simmental and charolais cattle
Journal of Animal Science, 1995Co-Authors: J.d. Ward, J W Spears, G P GengelbachAbstract:: Thirty-eight pregnant Angus, Charolais, and Simmental heifers and their offspring were used to determine whether differences in Cu metabolism existed among breeds. The heifers were fed either 0 or 10 mg of supplemental Cu per kilogram of DM. Calves were born between d 70 and 125 of the 280 d experiment. There were few differences among breeds in plasma Cu concentration and ceruloplasmin activity when heifers were fed supplemental Cu. When heifers were not fed supplemental Cu, Angus heifers had greater (P < .10) plasma Cu concentrations than Simmental heifers from d 0 until 140 and Charolais heifers from d 28 until 140. Angus heifers had greater (P < .10) ceruloplasmin activity than Charolais and Simmental heifers between d 28 and 196. When dams were fed supplemental Cu, Angus calves had greater (P < .10) plasma Cu concentrations than Simmental calves on all days tested and Charolais calves on d 252 and 280. Ceruloplasmin activity of Angus calves in the Cu-supplemented group was greater (P < .10) than that of Charolais calves on d 224 and 280 and Simmental calves from d 224 until 280. Angus calves born to dams not receiving supplemental Cu had greater (P < .10) plasma Cu concentrations and ceruloplasmin activities than Simmental calves on all days tested. Angus calves also had greater (P < .10) plasma Cu concentrations than Charolais calves on d 196 and greater ceruloplasmin activities than Charolais calves from d 168 until 224. Eight Angus and eight Simmental steers were used to evaluate apparent Cu absorption and retention.(ABSTRACT TRUNCATED AT 250 WORDS)
