The Experts below are selected from a list of 174774 Experts worldwide ranked by ideXlab platform
Min Xue - One of the best experts on this subject based on the ideXlab platform.
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Fluorescence imaging-based methods for Single-Cell Protein analysis
Analytical and Bioanalytical Chemistry, 2019Co-Authors: Siwen Wang, Fei Ji, Zhonghan Li, Min XueAbstract:The quantity and activity of Proteins in many biological systems exhibit prominent heterogeneities. Single-Cell analytical methods can resolve subpopulations and dissect their unique signatures from heterogeneous samples, enabling a clarifying view of the biological process. Over the last 5 years, technologies for Single-Cell Protein analysis have significantly advanced. In this article, we highlight a branch of those technology developments involving fluorescence-based approaches, with a focus on the methods that increase the ability to multiplex and enable dynamic measurements. We also analyze the limitations of these techniques and discuss current challenges in the field, with the hope that more transformative platforms can soon emerge.
Nikolai Slavov - One of the best experts on this subject based on the ideXlab platform.
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Single Cell Protein analysis for systems biology
Essays in Biochemistry, 2018Co-Authors: Ezra Levy, Nikolai SlavovAbstract:The Cellular abundance of Proteins can vary even between isogenic Single Cells. This variability between Single-Cell Protein levels can have regulatory roles, such as controlling Cell fate during apoptosis induction or the proliferation/quiescence decision. Here, we review examples connecting Protein levels and their dynamics in Single Cells to Cellular functions. Such findings were made possible by the introduction of antibodies, and subsequently fluorescent Proteins, for tracking Protein levels in Single Cells. However, in heterogeneous Cell populations, such as tumors or differentiating stem Cells, Cellular decisions are controlled by hundreds, even thousands of Proteins acting in concert. Characterizing such complex systems demands measurements of thousands of Proteins across thousands of Single Cells. This demand has inspired the development of new methods for Single-Cell Protein analysis, and we discuss their trade-offs, with an emphasis on their specificity and coverage. We finish by highlighting the potential of emerging mass-spec methods to enable systems-level measurement of Single-Cell proteomes with unprecedented coverage and specificity. Combining such methods with methods for quantitating the transcriptomes and metabolomes of Single Cells will provide essential data for advancing quantitative systems biology.
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Single Cell Protein analysis for systems biology
2018Co-Authors: Ezra Levy, Nikolai SlavovAbstract:The Cellular abundance of Proteins can vary even between isogenic Single Cells. This variability between Single-Cell Protein levels can have functional roles, such as controlling Cell fate during apoptosis induction or the proliferation/quiescence decision. Here, we review such examples of connecting Protein levels and their dynamics in Single Cells to Cellular functions. Such findings were made possible by the introduction of antibodies, and subsequently fluorescent Proteins, for tracking Protein levels in Single Cells. However, in heterogeneous Cell populations, such as tumors or differentiating stem Cells, Cellular decisions are controlled by hundreds, even thousands of Proteins acting in concert. Characterizing such complex systems demands measurements of thousands of Proteins across thousands of Single Cells. This demand has inspired the development of new methods for Single Cell Protein analysis, and we discuss their trade-offs, with emphasis on their specificity and coverage. We finish by highlighting the potential of emerging mass-spec methods to enable systems-level measurement of Single-Cell proteomes with unprecedented coverage and specificity. Combining such methods with methods for quantifying the trasncriptomes and metabolomes of Single Cells will provide essential data for advancing quantitative systems biology.
Siwen Wang - One of the best experts on this subject based on the ideXlab platform.
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Fluorescence imaging-based methods for Single-Cell Protein analysis
Analytical and Bioanalytical Chemistry, 2019Co-Authors: Siwen Wang, Fei Ji, Zhonghan Li, Min XueAbstract:The quantity and activity of Proteins in many biological systems exhibit prominent heterogeneities. Single-Cell analytical methods can resolve subpopulations and dissect their unique signatures from heterogeneous samples, enabling a clarifying view of the biological process. Over the last 5 years, technologies for Single-Cell Protein analysis have significantly advanced. In this article, we highlight a branch of those technology developments involving fluorescence-based approaches, with a focus on the methods that increase the ability to multiplex and enable dynamic measurements. We also analyze the limitations of these techniques and discuss current challenges in the field, with the hope that more transformative platforms can soon emerge.
Zhuang Gui - One of the best experts on this subject based on the ideXlab platform.
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Study on brewing soy sauce with yeast Single Cell Protein produced by saccharifarified residue of vinegar
China Brewing, 2005Co-Authors: Zhuang GuiAbstract:After saccharifying residue of bran koji vinegar through multi-strain culture fermentation,the reducing sugar content of saccharified vinegar residue was as high as 27.2 %.The yeast Single Cell Protein material was obtained by saccharified vinegar residue residues.The number of the yeast Cell and the organic nitrogen content of the material reached up to 1.26×109 cfu g-1(dry matter)and 4.76%(dry weight),respectively.The soy sauce according with the quality standard of GB18186-2000 was then produced by using yeast Single Cell Protein as raw material and fermented with koji by low-salt and solid-state fermentation.
Ezra Levy - One of the best experts on this subject based on the ideXlab platform.
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Single Cell Protein analysis for systems biology
Essays in Biochemistry, 2018Co-Authors: Ezra Levy, Nikolai SlavovAbstract:The Cellular abundance of Proteins can vary even between isogenic Single Cells. This variability between Single-Cell Protein levels can have regulatory roles, such as controlling Cell fate during apoptosis induction or the proliferation/quiescence decision. Here, we review examples connecting Protein levels and their dynamics in Single Cells to Cellular functions. Such findings were made possible by the introduction of antibodies, and subsequently fluorescent Proteins, for tracking Protein levels in Single Cells. However, in heterogeneous Cell populations, such as tumors or differentiating stem Cells, Cellular decisions are controlled by hundreds, even thousands of Proteins acting in concert. Characterizing such complex systems demands measurements of thousands of Proteins across thousands of Single Cells. This demand has inspired the development of new methods for Single-Cell Protein analysis, and we discuss their trade-offs, with an emphasis on their specificity and coverage. We finish by highlighting the potential of emerging mass-spec methods to enable systems-level measurement of Single-Cell proteomes with unprecedented coverage and specificity. Combining such methods with methods for quantitating the transcriptomes and metabolomes of Single Cells will provide essential data for advancing quantitative systems biology.
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Single Cell Protein analysis for systems biology
2018Co-Authors: Ezra Levy, Nikolai SlavovAbstract:The Cellular abundance of Proteins can vary even between isogenic Single Cells. This variability between Single-Cell Protein levels can have functional roles, such as controlling Cell fate during apoptosis induction or the proliferation/quiescence decision. Here, we review such examples of connecting Protein levels and their dynamics in Single Cells to Cellular functions. Such findings were made possible by the introduction of antibodies, and subsequently fluorescent Proteins, for tracking Protein levels in Single Cells. However, in heterogeneous Cell populations, such as tumors or differentiating stem Cells, Cellular decisions are controlled by hundreds, even thousands of Proteins acting in concert. Characterizing such complex systems demands measurements of thousands of Proteins across thousands of Single Cells. This demand has inspired the development of new methods for Single Cell Protein analysis, and we discuss their trade-offs, with emphasis on their specificity and coverage. We finish by highlighting the potential of emerging mass-spec methods to enable systems-level measurement of Single-Cell proteomes with unprecedented coverage and specificity. Combining such methods with methods for quantifying the trasncriptomes and metabolomes of Single Cells will provide essential data for advancing quantitative systems biology.
