The Experts below are selected from a list of 24 Experts worldwide ranked by ideXlab platform

Marco Tartagni - One of the best experts on this subject based on the ideXlab platform.

  • A Miniaturized Single Channel Amplifier for Various Different Electrophysiology Setup
    Biophysical Journal, 2014
    Co-Authors: Federico Thei, Michele Rossi, Marco Bennati, Marco Crescentini, Marco Tartagni
    Abstract:

    Different electrophysiology setups allow Single ion Channel recordings using Lipid Bilayer Membrane (BLM) for protein insertion. Ion Channels are transmembrane proteins involved in many biological process of cell life. They are used as target for drug discovery and Single-molecule sensors. Synthetic nanopores can also be used as nano-coulter counters and very promising transducers for DNA sequencing. Complex protocols are under investigation to assembly ion Channels on reconstituted lipid membranes. They use different setups, ranging from suspended BLM, microfluidic and lab-on-a-chip devices, planar patch clamp, tip-dip method. These studies, combined to new emerging nanopore applications, require high sensitive and high resolution instrumentation, able to detect small currents (in the pA range) over large bandwith (up to 100kHz), providing flexible voltage stimuli generation. We present a compact and complete system for Single ion Channels and synthetic nanopores experiment, useful for scientists and students for academic purposes, composed by:- a miniaturized low noise Single Channel voltage-clamp Amplifier and digitizer integrated into a Single ASIC, with direct connection to a computer USB port;- a software interface for real time data display and analysis featuring flexible voltage stimuli control;- customised electrode interfaces for various different electrophysiology platforms and microfluidic devices.As a proof of concept, we demonstrate the system performances using different test proteins (KcsA, Gramicidin, Alpha-hemolysin) embedded into reconstituted lipid membranes. The acquired data are presented.

Federico Thei - One of the best experts on this subject based on the ideXlab platform.

  • A Miniaturized Single Channel Amplifier for Various Different Electrophysiology Setup
    Biophysical Journal, 2014
    Co-Authors: Federico Thei, Michele Rossi, Marco Bennati, Marco Crescentini, Marco Tartagni
    Abstract:

    Different electrophysiology setups allow Single ion Channel recordings using Lipid Bilayer Membrane (BLM) for protein insertion. Ion Channels are transmembrane proteins involved in many biological process of cell life. They are used as target for drug discovery and Single-molecule sensors. Synthetic nanopores can also be used as nano-coulter counters and very promising transducers for DNA sequencing. Complex protocols are under investigation to assembly ion Channels on reconstituted lipid membranes. They use different setups, ranging from suspended BLM, microfluidic and lab-on-a-chip devices, planar patch clamp, tip-dip method. These studies, combined to new emerging nanopore applications, require high sensitive and high resolution instrumentation, able to detect small currents (in the pA range) over large bandwith (up to 100kHz), providing flexible voltage stimuli generation. We present a compact and complete system for Single ion Channels and synthetic nanopores experiment, useful for scientists and students for academic purposes, composed by:- a miniaturized low noise Single Channel voltage-clamp Amplifier and digitizer integrated into a Single ASIC, with direct connection to a computer USB port;- a software interface for real time data display and analysis featuring flexible voltage stimuli control;- customised electrode interfaces for various different electrophysiology platforms and microfluidic devices.As a proof of concept, we demonstrate the system performances using different test proteins (KcsA, Gramicidin, Alpha-hemolysin) embedded into reconstituted lipid membranes. The acquired data are presented.

Marco Bennati - One of the best experts on this subject based on the ideXlab platform.

  • A Miniaturized Single Channel Amplifier for Various Different Electrophysiology Setup
    Biophysical Journal, 2014
    Co-Authors: Federico Thei, Michele Rossi, Marco Bennati, Marco Crescentini, Marco Tartagni
    Abstract:

    Different electrophysiology setups allow Single ion Channel recordings using Lipid Bilayer Membrane (BLM) for protein insertion. Ion Channels are transmembrane proteins involved in many biological process of cell life. They are used as target for drug discovery and Single-molecule sensors. Synthetic nanopores can also be used as nano-coulter counters and very promising transducers for DNA sequencing. Complex protocols are under investigation to assembly ion Channels on reconstituted lipid membranes. They use different setups, ranging from suspended BLM, microfluidic and lab-on-a-chip devices, planar patch clamp, tip-dip method. These studies, combined to new emerging nanopore applications, require high sensitive and high resolution instrumentation, able to detect small currents (in the pA range) over large bandwith (up to 100kHz), providing flexible voltage stimuli generation. We present a compact and complete system for Single ion Channels and synthetic nanopores experiment, useful for scientists and students for academic purposes, composed by:- a miniaturized low noise Single Channel voltage-clamp Amplifier and digitizer integrated into a Single ASIC, with direct connection to a computer USB port;- a software interface for real time data display and analysis featuring flexible voltage stimuli control;- customised electrode interfaces for various different electrophysiology platforms and microfluidic devices.As a proof of concept, we demonstrate the system performances using different test proteins (KcsA, Gramicidin, Alpha-hemolysin) embedded into reconstituted lipid membranes. The acquired data are presented.

Marco Crescentini - One of the best experts on this subject based on the ideXlab platform.

  • A Miniaturized Single Channel Amplifier for Various Different Electrophysiology Setup
    Biophysical Journal, 2014
    Co-Authors: Federico Thei, Michele Rossi, Marco Bennati, Marco Crescentini, Marco Tartagni
    Abstract:

    Different electrophysiology setups allow Single ion Channel recordings using Lipid Bilayer Membrane (BLM) for protein insertion. Ion Channels are transmembrane proteins involved in many biological process of cell life. They are used as target for drug discovery and Single-molecule sensors. Synthetic nanopores can also be used as nano-coulter counters and very promising transducers for DNA sequencing. Complex protocols are under investigation to assembly ion Channels on reconstituted lipid membranes. They use different setups, ranging from suspended BLM, microfluidic and lab-on-a-chip devices, planar patch clamp, tip-dip method. These studies, combined to new emerging nanopore applications, require high sensitive and high resolution instrumentation, able to detect small currents (in the pA range) over large bandwith (up to 100kHz), providing flexible voltage stimuli generation. We present a compact and complete system for Single ion Channels and synthetic nanopores experiment, useful for scientists and students for academic purposes, composed by:- a miniaturized low noise Single Channel voltage-clamp Amplifier and digitizer integrated into a Single ASIC, with direct connection to a computer USB port;- a software interface for real time data display and analysis featuring flexible voltage stimuli control;- customised electrode interfaces for various different electrophysiology platforms and microfluidic devices.As a proof of concept, we demonstrate the system performances using different test proteins (KcsA, Gramicidin, Alpha-hemolysin) embedded into reconstituted lipid membranes. The acquired data are presented.

Michele Rossi - One of the best experts on this subject based on the ideXlab platform.

  • A Miniaturized Single Channel Amplifier for Various Different Electrophysiology Setup
    Biophysical Journal, 2014
    Co-Authors: Federico Thei, Michele Rossi, Marco Bennati, Marco Crescentini, Marco Tartagni
    Abstract:

    Different electrophysiology setups allow Single ion Channel recordings using Lipid Bilayer Membrane (BLM) for protein insertion. Ion Channels are transmembrane proteins involved in many biological process of cell life. They are used as target for drug discovery and Single-molecule sensors. Synthetic nanopores can also be used as nano-coulter counters and very promising transducers for DNA sequencing. Complex protocols are under investigation to assembly ion Channels on reconstituted lipid membranes. They use different setups, ranging from suspended BLM, microfluidic and lab-on-a-chip devices, planar patch clamp, tip-dip method. These studies, combined to new emerging nanopore applications, require high sensitive and high resolution instrumentation, able to detect small currents (in the pA range) over large bandwith (up to 100kHz), providing flexible voltage stimuli generation. We present a compact and complete system for Single ion Channels and synthetic nanopores experiment, useful for scientists and students for academic purposes, composed by:- a miniaturized low noise Single Channel voltage-clamp Amplifier and digitizer integrated into a Single ASIC, with direct connection to a computer USB port;- a software interface for real time data display and analysis featuring flexible voltage stimuli control;- customised electrode interfaces for various different electrophysiology platforms and microfluidic devices.As a proof of concept, we demonstrate the system performances using different test proteins (KcsA, Gramicidin, Alpha-hemolysin) embedded into reconstituted lipid membranes. The acquired data are presented.