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John Brann - One of the best experts on this subject based on the ideXlab platform.

  • description and validation of a staggered parallel high performance liquid chromatography System for good laboratory practice level quantitative analysis by liquid chromatography tandem mass spectrometry
    Rapid Communications in Mass Spectrometry, 2002
    Co-Authors: Richard C King, Daniel J Magiera, Cynthia Millerstein, John Brann
    Abstract:

    The Aria LX4 staggered parallel high performance liquid chromatography (HPLC) System is evaluated for application to good laboratory practice (GLP) level quantitative analysis by liquid chromatography/tandem mass spectrometry (LC/MS/MS). This System consists of four fully independent binary HPLC pumps, a modified autosampler, and a series of switching and selector valves all controlled by a Single computer program. The System improves sample throughput without sacrificing chromatographic separation or data quality. Validation results for four different compounds, each analyzed on a separate Channel of the Aria System, show precision and accuracy equivalent to that required of a Single-Channel System. The results show that sample throughput can be increased nearly four-fold without requiring significant changes in current analytical procedures. The flexibility and ease of use of the Aria System suggest that it should be possible to quickly implement it in any analytical LC/MS/MS environment. Copyright © 2001 John Wiley & Sons, Ltd.

  • description and validation of a staggered parallel high performance liquid chromatography System for good laboratory practice level quantitative analysis by liquid chromatography tandem mass spectrometry
    Rapid Communications in Mass Spectrometry, 2002
    Co-Authors: Richard C King, Daniel J Magiera, Cynthia Millerstein, John Brann
    Abstract:

    The Aria LX4 staggered parallel high performance liquid chromatography (HPLC) System is evaluated for application to good laboratory practice (GLP) level quantitative analysis by liquid chromatography/tandem mass spectrometry (LC/MS/MS). This System consists of four fully independent binary HPLC pumps, a modified autosampler, and a series of switching and selector valves all controlled by a Single computer program. The System improves sample throughput without sacrificing chromatographic separation or data quality. Validation results for four different compounds, each analyzed on a separate Channel of the Aria System, show precision and accuracy equivalent to that required of a Single-Channel System. The results show that sample throughput can be increased nearly four-fold without requiring significant changes in current analytical procedures. The flexibility and ease of use of the Aria System suggest that it should be possible to quickly implement it in any analytical LC/MS/MS environment.

Richard C King - One of the best experts on this subject based on the ideXlab platform.

  • description and validation of a staggered parallel high performance liquid chromatography System for good laboratory practice level quantitative analysis by liquid chromatography tandem mass spectrometry
    Rapid Communications in Mass Spectrometry, 2002
    Co-Authors: Richard C King, Daniel J Magiera, Cynthia Millerstein, John Brann
    Abstract:

    The Aria LX4 staggered parallel high performance liquid chromatography (HPLC) System is evaluated for application to good laboratory practice (GLP) level quantitative analysis by liquid chromatography/tandem mass spectrometry (LC/MS/MS). This System consists of four fully independent binary HPLC pumps, a modified autosampler, and a series of switching and selector valves all controlled by a Single computer program. The System improves sample throughput without sacrificing chromatographic separation or data quality. Validation results for four different compounds, each analyzed on a separate Channel of the Aria System, show precision and accuracy equivalent to that required of a Single-Channel System. The results show that sample throughput can be increased nearly four-fold without requiring significant changes in current analytical procedures. The flexibility and ease of use of the Aria System suggest that it should be possible to quickly implement it in any analytical LC/MS/MS environment. Copyright © 2001 John Wiley & Sons, Ltd.

  • description and validation of a staggered parallel high performance liquid chromatography System for good laboratory practice level quantitative analysis by liquid chromatography tandem mass spectrometry
    Rapid Communications in Mass Spectrometry, 2002
    Co-Authors: Richard C King, Daniel J Magiera, Cynthia Millerstein, John Brann
    Abstract:

    The Aria LX4 staggered parallel high performance liquid chromatography (HPLC) System is evaluated for application to good laboratory practice (GLP) level quantitative analysis by liquid chromatography/tandem mass spectrometry (LC/MS/MS). This System consists of four fully independent binary HPLC pumps, a modified autosampler, and a series of switching and selector valves all controlled by a Single computer program. The System improves sample throughput without sacrificing chromatographic separation or data quality. Validation results for four different compounds, each analyzed on a separate Channel of the Aria System, show precision and accuracy equivalent to that required of a Single-Channel System. The results show that sample throughput can be increased nearly four-fold without requiring significant changes in current analytical procedures. The flexibility and ease of use of the Aria System suggest that it should be possible to quickly implement it in any analytical LC/MS/MS environment.

Daniel J Magiera - One of the best experts on this subject based on the ideXlab platform.

  • description and validation of a staggered parallel high performance liquid chromatography System for good laboratory practice level quantitative analysis by liquid chromatography tandem mass spectrometry
    Rapid Communications in Mass Spectrometry, 2002
    Co-Authors: Richard C King, Daniel J Magiera, Cynthia Millerstein, John Brann
    Abstract:

    The Aria LX4 staggered parallel high performance liquid chromatography (HPLC) System is evaluated for application to good laboratory practice (GLP) level quantitative analysis by liquid chromatography/tandem mass spectrometry (LC/MS/MS). This System consists of four fully independent binary HPLC pumps, a modified autosampler, and a series of switching and selector valves all controlled by a Single computer program. The System improves sample throughput without sacrificing chromatographic separation or data quality. Validation results for four different compounds, each analyzed on a separate Channel of the Aria System, show precision and accuracy equivalent to that required of a Single-Channel System. The results show that sample throughput can be increased nearly four-fold without requiring significant changes in current analytical procedures. The flexibility and ease of use of the Aria System suggest that it should be possible to quickly implement it in any analytical LC/MS/MS environment. Copyright © 2001 John Wiley & Sons, Ltd.

  • description and validation of a staggered parallel high performance liquid chromatography System for good laboratory practice level quantitative analysis by liquid chromatography tandem mass spectrometry
    Rapid Communications in Mass Spectrometry, 2002
    Co-Authors: Richard C King, Daniel J Magiera, Cynthia Millerstein, John Brann
    Abstract:

    The Aria LX4 staggered parallel high performance liquid chromatography (HPLC) System is evaluated for application to good laboratory practice (GLP) level quantitative analysis by liquid chromatography/tandem mass spectrometry (LC/MS/MS). This System consists of four fully independent binary HPLC pumps, a modified autosampler, and a series of switching and selector valves all controlled by a Single computer program. The System improves sample throughput without sacrificing chromatographic separation or data quality. Validation results for four different compounds, each analyzed on a separate Channel of the Aria System, show precision and accuracy equivalent to that required of a Single-Channel System. The results show that sample throughput can be increased nearly four-fold without requiring significant changes in current analytical procedures. The flexibility and ease of use of the Aria System suggest that it should be possible to quickly implement it in any analytical LC/MS/MS environment.

Cynthia Millerstein - One of the best experts on this subject based on the ideXlab platform.

  • description and validation of a staggered parallel high performance liquid chromatography System for good laboratory practice level quantitative analysis by liquid chromatography tandem mass spectrometry
    Rapid Communications in Mass Spectrometry, 2002
    Co-Authors: Richard C King, Daniel J Magiera, Cynthia Millerstein, John Brann
    Abstract:

    The Aria LX4 staggered parallel high performance liquid chromatography (HPLC) System is evaluated for application to good laboratory practice (GLP) level quantitative analysis by liquid chromatography/tandem mass spectrometry (LC/MS/MS). This System consists of four fully independent binary HPLC pumps, a modified autosampler, and a series of switching and selector valves all controlled by a Single computer program. The System improves sample throughput without sacrificing chromatographic separation or data quality. Validation results for four different compounds, each analyzed on a separate Channel of the Aria System, show precision and accuracy equivalent to that required of a Single-Channel System. The results show that sample throughput can be increased nearly four-fold without requiring significant changes in current analytical procedures. The flexibility and ease of use of the Aria System suggest that it should be possible to quickly implement it in any analytical LC/MS/MS environment. Copyright © 2001 John Wiley & Sons, Ltd.

  • description and validation of a staggered parallel high performance liquid chromatography System for good laboratory practice level quantitative analysis by liquid chromatography tandem mass spectrometry
    Rapid Communications in Mass Spectrometry, 2002
    Co-Authors: Richard C King, Daniel J Magiera, Cynthia Millerstein, John Brann
    Abstract:

    The Aria LX4 staggered parallel high performance liquid chromatography (HPLC) System is evaluated for application to good laboratory practice (GLP) level quantitative analysis by liquid chromatography/tandem mass spectrometry (LC/MS/MS). This System consists of four fully independent binary HPLC pumps, a modified autosampler, and a series of switching and selector valves all controlled by a Single computer program. The System improves sample throughput without sacrificing chromatographic separation or data quality. Validation results for four different compounds, each analyzed on a separate Channel of the Aria System, show precision and accuracy equivalent to that required of a Single-Channel System. The results show that sample throughput can be increased nearly four-fold without requiring significant changes in current analytical procedures. The flexibility and ease of use of the Aria System suggest that it should be possible to quickly implement it in any analytical LC/MS/MS environment.

Dino Di Carlo - One of the best experts on this subject based on the ideXlab platform.

  • continuous scalable blood filtration device using inertial microfluidics
    Biotechnology and Bioengineering, 2010
    Co-Authors: Albert J. Mach, Dino Di Carlo
    Abstract:

    Cell separation is broadly useful for applications in clinical diagnostics, biological research, and potentially regenerative medicine. Recent attention has been paid to label-free size-based techniques that may avoid the costs or clogging issues associated with centrifugation and mechanical filtration. We present for the first time a massively parallel microfluidic device that passively separates pathogenic bacteria cells from diluted blood with macroscale performance. The device was designed to process large sample volumes in a high-throughput, continuous manner using 40 Single microChannels placed in a radial array with one inlet and two rings of outlets. Each Single Channel consists of a short focusing, gradual expansion and collection region and uses unique differential transit times due to size-dependent inertial lift forces as a method of cell separation. The gradual Channel expansion region is shown to manipulate cell equilibrium positions close to the microChannel walls, critical for higher efficiency collection. We demonstrate >80% removal of pathogenic bacteria from blood after two passes of the Single Channel System. The massively parallel device can process 240 mL/h with a throughput of 400 million cells/min. We expect that this parallelizable, robust, and label-free approach would be useful for filtration of blood as well as for other cell separation and concentration applications from large volume samples. Biotechnol. Bioeng. 2010;107: 302–311. © 2010 Wiley Periodicals, Inc.

  • Continuous scalable blood filtration device using inertial microfluidics
    Biotechnology and Bioengineering, 2010
    Co-Authors: Albert J. Mach, Dino Di Carlo
    Abstract:

    Cell separation is broadly useful for applications in clinical diagnostics, biological research, and potentially regenerative medicine. Recent attention has been paid to label-free size-based techniques that may avoid the costs or clogging issues associated with centrifugation and mechanical filtration. We present for the first time a massively parallel microfluidic device that passively separates pathogenic bacteria cells from diluted blood with macroscale performance. The device was designed to process large sample volumes in a high-throughput, continuous manner using 40 Single microChannels placed in a radial array with one inlet and two rings of outlets. Each Single Channel consists of a short focusing, gradual expansion and collection region and uses unique differential transit times due to size-dependent inertial lift forces as a method of cell separation. The gradual Channel expansion region is shown to manipulate cell equilibrium positions close to the microChannel walls, critical for higher efficiency collection. We demonstrate >80% removal of pathogenic bacteria from blood after two passes of the Single Channel System. The massively parallel device can process 240 mL/h with a throughput of 400 million cells/min. We expect that this parallelizable, robust, and label-free approach would be useful for filtration of blood as well as for other cell separation and concentration applications from large volume samples.