The Experts below are selected from a list of 14193 Experts worldwide ranked by ideXlab platform
Peter X - One of the best experts on this subject based on the ideXlab platform.
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nanofiber yarn hydrogel core shell scaffolds mimicking native Skeletal Muscle tissue for guiding 3d myoblast alignment elongation and differentiation
ACS Nano, 2015Co-Authors: Yaobin Wu, Ling Wang, Peter XAbstract:Designing scaffolds that can mimic native Skeletal Muscle tissue and induce 3D cellular alignment and elongated myotube formation remains an ongoing challenge for Skeletal Muscle tissue engineering. Herein, we present a simple technique to generate core–shell composite scaffolds for mimicking native Skeletal Muscle Structure, which comprise the aligned nanofiber yarn (NFY) core and the photocurable hydrogel shell. The aligned NFYs are prepared by the hybrid composition including poly(caprolactone), silk fibroin, and polyaniline via a developed dry–wet electrospinning method. A series of core–shell column and sheet composite scaffolds are ultimately obtained by encapsulating a piece and layers of aligned NFY cores within the hydrogel shell after photo-cross-linking. C2C12 myoblasts are seeded within the core–shell scaffolds, and the good biocompatibility of these scaffolds and their ability to induce 3D cellular alignment and elongation are successfully demonstrated. Furthermore, the 3D elongated myotube f...
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nanofiber yarn hydrogel core shell scaffolds mimicking native Skeletal Muscle tissue for guiding 3d myoblast alignment elongation and differentiation
ACS Nano, 2015Co-Authors: Ling Wang, Baolin Guo, Peter XAbstract:Designing scaffolds that can mimic native Skeletal Muscle tissue and induce 3D cellular alignment and elongated myotube formation remains an ongoing challenge for Skeletal Muscle tissue engineering. Herein, we present a simple technique to generate core-shell composite scaffolds for mimicking native Skeletal Muscle Structure, which comprise the aligned nanofiber yarn (NFY) core and the photocurable hydrogel shell. The aligned NFYs are prepared by the hybrid composition including poly(caprolactone), silk fibroin, and polyaniline via a developed dry-wet electrospinning method. A series of core-shell column and sheet composite scaffolds are ultimately obtained by encapsulating a piece and layers of aligned NFY cores within the hydrogel shell after photo-cross-linking. C2C12 myoblasts are seeded within the core-shell scaffolds, and the good biocompatibility of these scaffolds and their ability to induce 3D cellular alignment and elongation are successfully demonstrated. Furthermore, the 3D elongated myotube formation within core-shell scaffolds is also performed after long-term cultivation. These data suggest that these core-shell scaffolds combine the aligned NFY core that guides the myoblast alignment and differentiation and the hydrogel shell that provides a suitable 3D environment for nutrition exchange and mechanical protection to perform a great practical application for Skeletal Muscle regeneration.
Ling Wang - One of the best experts on this subject based on the ideXlab platform.
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nanofiber yarn hydrogel core shell scaffolds mimicking native Skeletal Muscle tissue for guiding 3d myoblast alignment elongation and differentiation
ACS Nano, 2015Co-Authors: Yaobin Wu, Ling Wang, Peter XAbstract:Designing scaffolds that can mimic native Skeletal Muscle tissue and induce 3D cellular alignment and elongated myotube formation remains an ongoing challenge for Skeletal Muscle tissue engineering. Herein, we present a simple technique to generate core–shell composite scaffolds for mimicking native Skeletal Muscle Structure, which comprise the aligned nanofiber yarn (NFY) core and the photocurable hydrogel shell. The aligned NFYs are prepared by the hybrid composition including poly(caprolactone), silk fibroin, and polyaniline via a developed dry–wet electrospinning method. A series of core–shell column and sheet composite scaffolds are ultimately obtained by encapsulating a piece and layers of aligned NFY cores within the hydrogel shell after photo-cross-linking. C2C12 myoblasts are seeded within the core–shell scaffolds, and the good biocompatibility of these scaffolds and their ability to induce 3D cellular alignment and elongation are successfully demonstrated. Furthermore, the 3D elongated myotube f...
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nanofiber yarn hydrogel core shell scaffolds mimicking native Skeletal Muscle tissue for guiding 3d myoblast alignment elongation and differentiation
ACS Nano, 2015Co-Authors: Ling Wang, Baolin Guo, Peter XAbstract:Designing scaffolds that can mimic native Skeletal Muscle tissue and induce 3D cellular alignment and elongated myotube formation remains an ongoing challenge for Skeletal Muscle tissue engineering. Herein, we present a simple technique to generate core-shell composite scaffolds for mimicking native Skeletal Muscle Structure, which comprise the aligned nanofiber yarn (NFY) core and the photocurable hydrogel shell. The aligned NFYs are prepared by the hybrid composition including poly(caprolactone), silk fibroin, and polyaniline via a developed dry-wet electrospinning method. A series of core-shell column and sheet composite scaffolds are ultimately obtained by encapsulating a piece and layers of aligned NFY cores within the hydrogel shell after photo-cross-linking. C2C12 myoblasts are seeded within the core-shell scaffolds, and the good biocompatibility of these scaffolds and their ability to induce 3D cellular alignment and elongation are successfully demonstrated. Furthermore, the 3D elongated myotube formation within core-shell scaffolds is also performed after long-term cultivation. These data suggest that these core-shell scaffolds combine the aligned NFY core that guides the myoblast alignment and differentiation and the hydrogel shell that provides a suitable 3D environment for nutrition exchange and mechanical protection to perform a great practical application for Skeletal Muscle regeneration.
Mark S Miller - One of the best experts on this subject based on the ideXlab platform.
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Skeletal Muscle atrophy and dysfunction in breast cancer patients role for chemotherapy derived oxidant stress
American Journal of Physiology-cell Physiology, 2018Co-Authors: Blas A Guigni, Damien M Callahan, Timothy W Tourville, Mark S Miller, Brad R Fiske, Thomas B Voigt, Bethany R Korwinmihavics, Vikas Anathy, Kim Dittus, Michael J TothAbstract:How breast cancer and its treatments affect Skeletal Muscle is not well defined. To address this question, we assessed Skeletal Muscle Structure and protein expression in 13 women who were diagnose...
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moderate intensity resistance exercise alters Skeletal Muscle molecular and cellular Structure and function in inactive older adults with knee osteoarthritis
Journal of Applied Physiology, 2017Co-Authors: Damien M Callahan, Timothy W Tourville, Mark S Miller, James R Slauterbeck, Patrick D Savage, Philip A Ades, Anna Kaplan, Brad R Fiske, Bruce D BeynnonAbstract:High-intensity resistance exercise (REX) training increases physical capacity, in part, by improving Muscle cell size and function. Moderate-intensity REX, which is more feasible for many older adults with disease and/or disability, also increases physical function, but the mechanisms underlying such improvements are not understood. Therefore, we measured Skeletal Muscle Structure and function from the molecular to the tissue level in response to 14 wk of moderate-intensity REX in physically inactive older adults with knee osteoarthritis (n = 17; 70 ± 1 yr). Although REX training increased quadriceps Muscle cross-sectional area (CSA), average single-fiber CSA was unchanged because of reciprocal changes in myosin heavy chain (MHC) I and IIA fibers. Intermyofibrillar mitochondrial content increased with training because of increases in mitochondrial size in men, but not women, with no changes in subsarcolemmal mitochondria in either sex. REX increased whole Muscle contractile performance similarly in men and women. In contrast, adaptations in single-Muscle fiber force production per CSA (i.e., tension) and contractile velocity varied between men and women in a fiber type-dependent manner, with adaptations being explained at the molecular level by differential changes in myosin-actin cross-bridge kinetics and mechanics and single-fiber MHC protein expression. Our results are notable compared with studies of high-intensity REX because they show that the effects of moderate-intensity REX in older adults on Muscle fiber size/Structure and myofilament function are absent or modest. Moreover, our data highlight unique sex-specific adaptations due to differential cellular and subcellular structural and functional changes.NEW & NOTEWORTHY Moderate-intensity resistance training causes sex-specific adaptations in Skeletal Muscle Structure and function at the cellular and molecular levels in inactive older adult men and women with knee osteoarthritis. However, these responses were minimal compared with high-intensity resistance training. Thus adjuncts to moderate-intensity training need to be developed to correct underlying cellular and molecular structural and functional deficits that are at the root of impaired physical function in this mobility-limited population.
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moderate intensity resistance exercise alters Skeletal Muscle molecular and cellular Structure and function in inactive older adults with knee osteoarthritis
Journal of Applied Physiology, 2017Co-Authors: Damien M Callahan, Timothy W Tourville, Mark S Miller, James R Slauterbeck, Patrick D Savage, Philip A Ades, Anna Kaplan, Brad R Fiske, Bruce D BeynnonAbstract:Moderate-intensity resistance training causes sex-specific adaptations in Skeletal Muscle Structure and function at the cellular and molecular levels in inactive older adult men and women with knee...
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chronic disuse and Skeletal Muscle Structure in older adults sex specific differences and relationships to contractile function
American Journal of Physiology-cell Physiology, 2015Co-Authors: Damien M Callahan, Timothy W Tourville, Mark S Miller, Sarah B Hackett, Himani Sharma, Nicholas C Cruickshank, James R Slauterbeck, Patrick D Savage, Philip A Ades, David W MaughanAbstract:In older adults, we examined the effect of chronic Muscle disuse on Skeletal Muscle Structure at the tissue, cellular, organellar, and molecular levels and its relationship to Muscle function. Volu...
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resistance training alters Skeletal Muscle Structure and function in human heart failure effects at the tissue cellular and molecular levels
The Journal of Physiology, 2012Co-Authors: Michael J Toth, Mark S Miller, Philip A Ades, Peter Vanburen, Nicholas G Bedrin, Martin M Lewinter, Bradley M PalmerAbstract:Key points • Individuals suffering from chronic heart failure are less able to perform everyday tasks. • This physical disability is explained, in part, by Muscle weakness secondary to alterations in the proteins in Muscles that are necessary for Muscle contraction (myofilament proteins). • Weight training exercise increases Muscle strength and physical function in heart failure patients, but the mechanisms of these improvements is uncertain. • We show that resistance training improves Muscle strength through increased function of myofilament proteins. • These studies are important because they identify the molecular and cellular mechanisms whereby this type of training may promote beneficial changes in physical function in elderly individuals with heart failure.
Yaobin Wu - One of the best experts on this subject based on the ideXlab platform.
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nanofiber yarn hydrogel core shell scaffolds mimicking native Skeletal Muscle tissue for guiding 3d myoblast alignment elongation and differentiation
ACS Nano, 2015Co-Authors: Yaobin Wu, Ling Wang, Peter XAbstract:Designing scaffolds that can mimic native Skeletal Muscle tissue and induce 3D cellular alignment and elongated myotube formation remains an ongoing challenge for Skeletal Muscle tissue engineering. Herein, we present a simple technique to generate core–shell composite scaffolds for mimicking native Skeletal Muscle Structure, which comprise the aligned nanofiber yarn (NFY) core and the photocurable hydrogel shell. The aligned NFYs are prepared by the hybrid composition including poly(caprolactone), silk fibroin, and polyaniline via a developed dry–wet electrospinning method. A series of core–shell column and sheet composite scaffolds are ultimately obtained by encapsulating a piece and layers of aligned NFY cores within the hydrogel shell after photo-cross-linking. C2C12 myoblasts are seeded within the core–shell scaffolds, and the good biocompatibility of these scaffolds and their ability to induce 3D cellular alignment and elongation are successfully demonstrated. Furthermore, the 3D elongated myotube f...
Bruce D Beynnon - One of the best experts on this subject based on the ideXlab platform.
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moderate intensity resistance exercise alters Skeletal Muscle molecular and cellular Structure and function in inactive older adults with knee osteoarthritis
Journal of Applied Physiology, 2017Co-Authors: Damien M Callahan, Timothy W Tourville, Mark S Miller, James R Slauterbeck, Patrick D Savage, Philip A Ades, Anna Kaplan, Brad R Fiske, Bruce D BeynnonAbstract:High-intensity resistance exercise (REX) training increases physical capacity, in part, by improving Muscle cell size and function. Moderate-intensity REX, which is more feasible for many older adults with disease and/or disability, also increases physical function, but the mechanisms underlying such improvements are not understood. Therefore, we measured Skeletal Muscle Structure and function from the molecular to the tissue level in response to 14 wk of moderate-intensity REX in physically inactive older adults with knee osteoarthritis (n = 17; 70 ± 1 yr). Although REX training increased quadriceps Muscle cross-sectional area (CSA), average single-fiber CSA was unchanged because of reciprocal changes in myosin heavy chain (MHC) I and IIA fibers. Intermyofibrillar mitochondrial content increased with training because of increases in mitochondrial size in men, but not women, with no changes in subsarcolemmal mitochondria in either sex. REX increased whole Muscle contractile performance similarly in men and women. In contrast, adaptations in single-Muscle fiber force production per CSA (i.e., tension) and contractile velocity varied between men and women in a fiber type-dependent manner, with adaptations being explained at the molecular level by differential changes in myosin-actin cross-bridge kinetics and mechanics and single-fiber MHC protein expression. Our results are notable compared with studies of high-intensity REX because they show that the effects of moderate-intensity REX in older adults on Muscle fiber size/Structure and myofilament function are absent or modest. Moreover, our data highlight unique sex-specific adaptations due to differential cellular and subcellular structural and functional changes.NEW & NOTEWORTHY Moderate-intensity resistance training causes sex-specific adaptations in Skeletal Muscle Structure and function at the cellular and molecular levels in inactive older adult men and women with knee osteoarthritis. However, these responses were minimal compared with high-intensity resistance training. Thus adjuncts to moderate-intensity training need to be developed to correct underlying cellular and molecular structural and functional deficits that are at the root of impaired physical function in this mobility-limited population.
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moderate intensity resistance exercise alters Skeletal Muscle molecular and cellular Structure and function in inactive older adults with knee osteoarthritis
Journal of Applied Physiology, 2017Co-Authors: Damien M Callahan, Timothy W Tourville, Mark S Miller, James R Slauterbeck, Patrick D Savage, Philip A Ades, Anna Kaplan, Brad R Fiske, Bruce D BeynnonAbstract:Moderate-intensity resistance training causes sex-specific adaptations in Skeletal Muscle Structure and function at the cellular and molecular levels in inactive older adult men and women with knee...