The Experts below are selected from a list of 339 Experts worldwide ranked by ideXlab platform
Thomas J Webste - One of the best experts on this subject based on the ideXlab platform.
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a mechanism for the enhanced attachment and proliferation of fibroblasts on anodized 316l stainless steel with nano pit arrays
Journal of Biomedical Materials Research Part B, 2014Co-Authors: Atu Erca, Luting Liu, Katherine S Zieme, Thomas J WebsteAbstract:In this study, 316L stainless steel with tunable nanometer pit sizes (0, 25, 50, and 60 nm) were fabricated by an anodization procedure in an ethylene glycol electrolyte solution containing 5 vol % perchloric acid. The surface mor- phology and elemental composition of the 316L stainless steel were characterized by scanning electron microscopy (SEM), atomic force microscopy (AFM), and X-ray photoelec- tron spectroscopy (XPS). The nano-pit arrays on all of the 316L stainless steel samples were in a regular arrangement. The surface properties of the 316L stainless steel nano-pit surface showed improved wettability properties as compared with the untreated 316L stainless steel, as demonstrated by the lower contact angles which dropped from 83.0 � to 28.6 to 45.4 � . The anodized 316L stainless steel surfaces with 50 nm and 60 nm diameter pits were also more rough at the nano- scale. According to MTT assays, compared with unanodized (that is, nano-smooth) surfaces, the 50 and 60 nm diameter nano-pit surfaces dramatically enhanced initial human dermal fibroblast attachment and growth for up to 3 days in culture. Mechanistically, this study also provided the first evidence of greater select protein adsorption (specifically, vitronectin and fibronectin which have been shown to enhance fibroblast adhesion) on the anodized 316L stainless steel compared with unanodized stainless steel. Such nano-pit surfaces can be designed to support fibroblast growth and, thus, improve the use of 316L stainless steel for various implant applica- tions (such as for enhanced Skin Healing for amputee devices and for percutaneous implants). V
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enhanced attachment and proliferation of fibroblasts on anodized 316l stainless steel with nano pit arrays
MRS Online Proceedings Library Archive, 2014Co-Authors: Atu Erca, Luting Liu, Katherine S Zieme, Thomas J WebsteAbstract:The aim of this study was to prepare various sized nano-pits on 316 L stainless steel and examine their effects on the attachment and proliferation of fibroblasts. In this study, 316L stainless steel with tunable pit sizes (0, 25, 50, and 60 nm) were fabricated by an anodization procedure in an ethylene glycol electrolyte solution containing 5 vol.% perchloric acid. The surface morphology of 316L stainless steel were characterized by scanning electron microscopy (SEM). The nano-pit arrays on all the 316L stainless steel samples were in a regular arrangement. The surface properties of the 316L stainless steel nano-pit surface showed improved wettability properties as compared to the untreated 316L stainless steel. The nano-pit surfaces with 50 nm and 60 nm diameter were rougher at the nanoscale than other samples. The attachment and proliferation of fibroblasts were investigated for up to 3 days in culture using MTT assays. Compared to unanodized (that is, nano-smooth) and smooth surfaces, 50 and 60 nm diameter nano-pit surfaces dramatically enhanced the initial fibroblast attachment and growth up to 3 days in culture. The results reported in this study showed that the 50 and 60 nm nano-pit surfaces promoted fibroblast adhesion and proliferation by increasing the surface roughness and adsorption of fibronectin. Such nano-pit surfaces can be designed to support fibroblast growth and be important for improving the use of 316L stainless steel for various implant applications (such as for improved Skin Healing for amputee devices or for percutaneous implants).
M Dugall - One of the best experts on this subject based on the ideXlab platform.
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microcirculatory efficacy of topical treatment with aescin essential phospholipids gel on transcutaneous po2 in venous insufficiency
Angiology, 2004Co-Authors: M R Cesarone, G Belcaro, E Ippolito, A Ricci, M Ruffini, M DugallAbstract:A gel including aescin, essential phospholipids (EPL), and heparin (EG) has been used for many years for local treatment of venous, microcirculatory alterations (varicose veins, chronic venous insufficiency). Bruises, swelling, thrombophlebitis, and contusions are effectively treated with this compound. The aim of this study was the evaluation of the efficacy of the effects of an AEPL (aescin+EPL) gel on the microcirculation in subjects with chronic venous insufficiency, venous hypertension (CVH), and venous microangiopathy. Transcutaneous PO2 was measured at the perimalleolar region. After 2 weeks of treatment, all individual values (100%) were significantly increased (p<0.05). In all patients, PO2 increased, indicating a decrease in level of venous microangiopathy. Considering transcutaneous Skin PO2, treatment with AEPL in areas of venous microangiopathy is beneficial in the prevention of ulceration and improves Skin Healing.
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microcirculatory efficacy of topical treatment with aescin essential phospholipids gel in venous insufficiency and hypertension new clinical observations
Angiology, 2004Co-Authors: G Belcaro, M R Cesarone, M DugallAbstract:Aescin + essential phospholipids (AEPL) topical gels are used for local treatment of venous and microcirculatory alterations (varicose veins, chronic venous insufficiency). Bruises, swelling, thrombophlebitis, and contusions are effectively treated with AEPL. Active ingredients are escinate and essential phospholipids (EPL). The aim of this new study was the evaluation of the efficacy of the effects of AEPL gel on the microcirculation in subjects with chronic venous insufficiency, venous hypertension (CVH), and venous microangiopathy. Patients were assessed measuring Skin flux with laser-Doppler flowmetry (LDF). After 2 weeks of local treatment, all individual values (100%) were significantly decreased (p<0.05), indicating an improvement in the microcirculation. In all treated patients, flux decreased at least 30% (indicating a decrease in the level of venous microangiopathy) (p<0.05). Considering these observations, topical treatment with AEPL in areas of venous microangiopathy is beneficial, can prevent ulceration, and improves the Skin Healing processes.
G Belcaro - One of the best experts on this subject based on the ideXlab platform.
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microcirculatory efficacy of topical treatment with aescin essential phospholipids gel on transcutaneous po2 in venous insufficiency
Angiology, 2004Co-Authors: M R Cesarone, G Belcaro, E Ippolito, A Ricci, M Ruffini, M DugallAbstract:A gel including aescin, essential phospholipids (EPL), and heparin (EG) has been used for many years for local treatment of venous, microcirculatory alterations (varicose veins, chronic venous insufficiency). Bruises, swelling, thrombophlebitis, and contusions are effectively treated with this compound. The aim of this study was the evaluation of the efficacy of the effects of an AEPL (aescin+EPL) gel on the microcirculation in subjects with chronic venous insufficiency, venous hypertension (CVH), and venous microangiopathy. Transcutaneous PO2 was measured at the perimalleolar region. After 2 weeks of treatment, all individual values (100%) were significantly increased (p<0.05). In all patients, PO2 increased, indicating a decrease in level of venous microangiopathy. Considering transcutaneous Skin PO2, treatment with AEPL in areas of venous microangiopathy is beneficial in the prevention of ulceration and improves Skin Healing.
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microcirculatory efficacy of topical treatment with aescin essential phospholipids gel in venous insufficiency and hypertension new clinical observations
Angiology, 2004Co-Authors: G Belcaro, M R Cesarone, M DugallAbstract:Aescin + essential phospholipids (AEPL) topical gels are used for local treatment of venous and microcirculatory alterations (varicose veins, chronic venous insufficiency). Bruises, swelling, thrombophlebitis, and contusions are effectively treated with AEPL. Active ingredients are escinate and essential phospholipids (EPL). The aim of this new study was the evaluation of the efficacy of the effects of AEPL gel on the microcirculation in subjects with chronic venous insufficiency, venous hypertension (CVH), and venous microangiopathy. Patients were assessed measuring Skin flux with laser-Doppler flowmetry (LDF). After 2 weeks of local treatment, all individual values (100%) were significantly decreased (p<0.05), indicating an improvement in the microcirculation. In all treated patients, flux decreased at least 30% (indicating a decrease in the level of venous microangiopathy) (p<0.05). Considering these observations, topical treatment with AEPL in areas of venous microangiopathy is beneficial, can prevent ulceration, and improves the Skin Healing processes.
M R Cesarone - One of the best experts on this subject based on the ideXlab platform.
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microcirculatory efficacy of topical treatment with aescin essential phospholipids gel on transcutaneous po2 in venous insufficiency
Angiology, 2004Co-Authors: M R Cesarone, G Belcaro, E Ippolito, A Ricci, M Ruffini, M DugallAbstract:A gel including aescin, essential phospholipids (EPL), and heparin (EG) has been used for many years for local treatment of venous, microcirculatory alterations (varicose veins, chronic venous insufficiency). Bruises, swelling, thrombophlebitis, and contusions are effectively treated with this compound. The aim of this study was the evaluation of the efficacy of the effects of an AEPL (aescin+EPL) gel on the microcirculation in subjects with chronic venous insufficiency, venous hypertension (CVH), and venous microangiopathy. Transcutaneous PO2 was measured at the perimalleolar region. After 2 weeks of treatment, all individual values (100%) were significantly increased (p<0.05). In all patients, PO2 increased, indicating a decrease in level of venous microangiopathy. Considering transcutaneous Skin PO2, treatment with AEPL in areas of venous microangiopathy is beneficial in the prevention of ulceration and improves Skin Healing.
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microcirculatory efficacy of topical treatment with aescin essential phospholipids gel in venous insufficiency and hypertension new clinical observations
Angiology, 2004Co-Authors: G Belcaro, M R Cesarone, M DugallAbstract:Aescin + essential phospholipids (AEPL) topical gels are used for local treatment of venous and microcirculatory alterations (varicose veins, chronic venous insufficiency). Bruises, swelling, thrombophlebitis, and contusions are effectively treated with AEPL. Active ingredients are escinate and essential phospholipids (EPL). The aim of this new study was the evaluation of the efficacy of the effects of AEPL gel on the microcirculation in subjects with chronic venous insufficiency, venous hypertension (CVH), and venous microangiopathy. Patients were assessed measuring Skin flux with laser-Doppler flowmetry (LDF). After 2 weeks of local treatment, all individual values (100%) were significantly decreased (p<0.05), indicating an improvement in the microcirculation. In all treated patients, flux decreased at least 30% (indicating a decrease in the level of venous microangiopathy) (p<0.05). Considering these observations, topical treatment with AEPL in areas of venous microangiopathy is beneficial, can prevent ulceration, and improves the Skin Healing processes.
Atu Erca - One of the best experts on this subject based on the ideXlab platform.
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a mechanism for the enhanced attachment and proliferation of fibroblasts on anodized 316l stainless steel with nano pit arrays
Journal of Biomedical Materials Research Part B, 2014Co-Authors: Atu Erca, Luting Liu, Katherine S Zieme, Thomas J WebsteAbstract:In this study, 316L stainless steel with tunable nanometer pit sizes (0, 25, 50, and 60 nm) were fabricated by an anodization procedure in an ethylene glycol electrolyte solution containing 5 vol % perchloric acid. The surface mor- phology and elemental composition of the 316L stainless steel were characterized by scanning electron microscopy (SEM), atomic force microscopy (AFM), and X-ray photoelec- tron spectroscopy (XPS). The nano-pit arrays on all of the 316L stainless steel samples were in a regular arrangement. The surface properties of the 316L stainless steel nano-pit surface showed improved wettability properties as compared with the untreated 316L stainless steel, as demonstrated by the lower contact angles which dropped from 83.0 � to 28.6 to 45.4 � . The anodized 316L stainless steel surfaces with 50 nm and 60 nm diameter pits were also more rough at the nano- scale. According to MTT assays, compared with unanodized (that is, nano-smooth) surfaces, the 50 and 60 nm diameter nano-pit surfaces dramatically enhanced initial human dermal fibroblast attachment and growth for up to 3 days in culture. Mechanistically, this study also provided the first evidence of greater select protein adsorption (specifically, vitronectin and fibronectin which have been shown to enhance fibroblast adhesion) on the anodized 316L stainless steel compared with unanodized stainless steel. Such nano-pit surfaces can be designed to support fibroblast growth and, thus, improve the use of 316L stainless steel for various implant applica- tions (such as for enhanced Skin Healing for amputee devices and for percutaneous implants). V
-
enhanced attachment and proliferation of fibroblasts on anodized 316l stainless steel with nano pit arrays
MRS Online Proceedings Library Archive, 2014Co-Authors: Atu Erca, Luting Liu, Katherine S Zieme, Thomas J WebsteAbstract:The aim of this study was to prepare various sized nano-pits on 316 L stainless steel and examine their effects on the attachment and proliferation of fibroblasts. In this study, 316L stainless steel with tunable pit sizes (0, 25, 50, and 60 nm) were fabricated by an anodization procedure in an ethylene glycol electrolyte solution containing 5 vol.% perchloric acid. The surface morphology of 316L stainless steel were characterized by scanning electron microscopy (SEM). The nano-pit arrays on all the 316L stainless steel samples were in a regular arrangement. The surface properties of the 316L stainless steel nano-pit surface showed improved wettability properties as compared to the untreated 316L stainless steel. The nano-pit surfaces with 50 nm and 60 nm diameter were rougher at the nanoscale than other samples. The attachment and proliferation of fibroblasts were investigated for up to 3 days in culture using MTT assays. Compared to unanodized (that is, nano-smooth) and smooth surfaces, 50 and 60 nm diameter nano-pit surfaces dramatically enhanced the initial fibroblast attachment and growth up to 3 days in culture. The results reported in this study showed that the 50 and 60 nm nano-pit surfaces promoted fibroblast adhesion and proliferation by increasing the surface roughness and adsorption of fibronectin. Such nano-pit surfaces can be designed to support fibroblast growth and be important for improving the use of 316L stainless steel for various implant applications (such as for improved Skin Healing for amputee devices or for percutaneous implants).
