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E Carstens - One of the best experts on this subject based on the ideXlab platform.
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Spinal c-fos expression associated with spontaneous biting in a mouse model of dry Skin Pruritus.
Neuroscience letters, 2004Co-Authors: Hiroshi Nojima, Jason M Cuellar, Christopher T Simons, Mirela Iodi Carstens, E CarstensAbstract:As a model of dry Skin Pruritus in mice, one hind paw was treated twice daily with a mixture of acetone/diethylether/water (AEW); controls received water only. A protective collar prevented the animals from accessing the treatment area. At 16 days, the collar was removed and AEW-treated mice exhibited marked biting of the treated paw; the number and cumulative duration of bites was significantly greater than in controls. After 3 additional treatment days (collars intact), animals were perfused for c-fos immunohistochemistry. There was significantly more fos-like immunoreactivity in the ipsilateral lumbar spinal cord of AEW-treated animals, with the majority in superficial laminae. It is proposed that biting of the dry Skin reflects Pruritus, and that neurons predominantly in superficial laminae of the dorsal horn may signal itch sensation.
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c-fos expression in superficial dorsal horn of cervical spinal cord associated with spontaneous scratching in rats with dry Skin
Neuroscience letters, 2003Co-Authors: Hiroshi Nojima, Mirela Iodi Carstens, E CarstensAbstract:Using a dry Skin model in rats, we assessed spontaneous itch-related scratching behavior and associated c-fos expression in the superficial dorsal horn of the spinal cord. The number of spontaneous bouts of hind limb scratching directed toward the nape of the neck was significantly higher after 5 days of topical application of acetone-diethylether-water (AEW) compared to pre-treatment levels or to control animals treated with water only. In AEW-treated animals, neurons expressing Fos-like immunoreactivity (FLI) were observed in superficial laminae of the dorsal horn at C3-C5 levels. There was a significant, positive correlation between the number of neurons in lamina I expressing FLI and the number and cumulative duration of spontaneous scratching bouts. These results suggest that this model may be useful to assess mechanisms of dry Skin Pruritus, and that lamina I neurons are activated as a consequence of itching and/or scratching in this model.
Hiroshi Nojima - One of the best experts on this subject based on the ideXlab platform.
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Spinal c-fos expression associated with spontaneous biting in a mouse model of dry Skin Pruritus.
Neuroscience letters, 2004Co-Authors: Hiroshi Nojima, Jason M Cuellar, Christopher T Simons, Mirela Iodi Carstens, E CarstensAbstract:As a model of dry Skin Pruritus in mice, one hind paw was treated twice daily with a mixture of acetone/diethylether/water (AEW); controls received water only. A protective collar prevented the animals from accessing the treatment area. At 16 days, the collar was removed and AEW-treated mice exhibited marked biting of the treated paw; the number and cumulative duration of bites was significantly greater than in controls. After 3 additional treatment days (collars intact), animals were perfused for c-fos immunohistochemistry. There was significantly more fos-like immunoreactivity in the ipsilateral lumbar spinal cord of AEW-treated animals, with the majority in superficial laminae. It is proposed that biting of the dry Skin reflects Pruritus, and that neurons predominantly in superficial laminae of the dorsal horn may signal itch sensation.
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c-fos expression in superficial dorsal horn of cervical spinal cord associated with spontaneous scratching in rats with dry Skin
Neuroscience letters, 2003Co-Authors: Hiroshi Nojima, Mirela Iodi Carstens, E CarstensAbstract:Using a dry Skin model in rats, we assessed spontaneous itch-related scratching behavior and associated c-fos expression in the superficial dorsal horn of the spinal cord. The number of spontaneous bouts of hind limb scratching directed toward the nape of the neck was significantly higher after 5 days of topical application of acetone-diethylether-water (AEW) compared to pre-treatment levels or to control animals treated with water only. In AEW-treated animals, neurons expressing Fos-like immunoreactivity (FLI) were observed in superficial laminae of the dorsal horn at C3-C5 levels. There was a significant, positive correlation between the number of neurons in lamina I expressing FLI and the number and cumulative duration of spontaneous scratching bouts. These results suggest that this model may be useful to assess mechanisms of dry Skin Pruritus, and that lamina I neurons are activated as a consequence of itching and/or scratching in this model.
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Itch-associated response induced by experimental dry Skin in mice.
Japanese journal of pharmacology, 2002Co-Authors: Takayuki Miyamoto, Hiroshi Nojima, Tomoko Shinkado, Takashi Nakahashi, Yasushi KuraishiAbstract:The present study was conducted to establish a new mouse model of dry Skin Pruritus. The rostral back was treated daily with cutaneous application of acetone/ether (1:1) mixture (AE), water following AE (AEW), 1% sodium lauryl sulfate (SLS) or tape stripping (TS). On the day after 5-day treatment, although all four treatments significantly decreased stratum corneum (SC) hydration and increased transepidermal water loss (TEWL), only AEW treatment significantly increased spontaneous scratching. An increase in the frequency of TS produced the marked increase of TEWL, without significant effects on SC hydration and spontaneous scratching. In AEW-treated mice, changes in SC hydration and TEWL were marked in the initial 2-day period, while spontaneous scratching increased gradually from 3 days after starting the treatment. The degranulation of cutaneous mast cells was increased by SLS treatment but not by other treatments. There was no apparent difference in AEW-induced spontaneous scratching between mast cell-deficient mice (WBB6F1-W/Wv) and normal littermates (WBB6F1-+/+). Opioid antagonists, naloxone and naltrexone, (1 mg/kg, subcutaneously) significantly suppressed spontaneous scratching in AEW-treated mice. It is suggested that spontaneous scratching of AEW-treated mice is an itch-related response and a useful model for studying the mechanisms of dry Skin Pruritus.
P C Ng - One of the best experts on this subject based on the ideXlab platform.
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comparison of Skin hydration evaluation sites and correlations among Skin hydration transepidermal water loss scorad index nottingham eczema severity score and quality of life in patients with atopic dermatitis
American Journal of Clinical Dermatology, 2008Co-Authors: Kin Yee Wong, Tingfan Leung, Chungmo Chow, P C NgAbstract:Background:Atopic dermatitis (AD) is characterized by dryness of the Skin, Pruritus and involvement of the Skin flexures. Skin hydration (SH) and integrity, as measured by transepidermal water loss (TEWL), are important parameters for objectively quantifying AD research.
Kin Yee Wong - One of the best experts on this subject based on the ideXlab platform.
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comparison of Skin hydration evaluation sites and correlations among Skin hydration transepidermal water loss scorad index nottingham eczema severity score and quality of life in patients with atopic dermatitis
American Journal of Clinical Dermatology, 2008Co-Authors: Kin Yee Wong, Tingfan Leung, Chungmo Chow, P C NgAbstract:Background:Atopic dermatitis (AD) is characterized by dryness of the Skin, Pruritus and involvement of the Skin flexures. Skin hydration (SH) and integrity, as measured by transepidermal water loss (TEWL), are important parameters for objectively quantifying AD research.
Mirela Iodi Carstens - One of the best experts on this subject based on the ideXlab platform.
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Spinal c-fos expression associated with spontaneous biting in a mouse model of dry Skin Pruritus.
Neuroscience letters, 2004Co-Authors: Hiroshi Nojima, Jason M Cuellar, Christopher T Simons, Mirela Iodi Carstens, E CarstensAbstract:As a model of dry Skin Pruritus in mice, one hind paw was treated twice daily with a mixture of acetone/diethylether/water (AEW); controls received water only. A protective collar prevented the animals from accessing the treatment area. At 16 days, the collar was removed and AEW-treated mice exhibited marked biting of the treated paw; the number and cumulative duration of bites was significantly greater than in controls. After 3 additional treatment days (collars intact), animals were perfused for c-fos immunohistochemistry. There was significantly more fos-like immunoreactivity in the ipsilateral lumbar spinal cord of AEW-treated animals, with the majority in superficial laminae. It is proposed that biting of the dry Skin reflects Pruritus, and that neurons predominantly in superficial laminae of the dorsal horn may signal itch sensation.
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c-fos expression in superficial dorsal horn of cervical spinal cord associated with spontaneous scratching in rats with dry Skin
Neuroscience letters, 2003Co-Authors: Hiroshi Nojima, Mirela Iodi Carstens, E CarstensAbstract:Using a dry Skin model in rats, we assessed spontaneous itch-related scratching behavior and associated c-fos expression in the superficial dorsal horn of the spinal cord. The number of spontaneous bouts of hind limb scratching directed toward the nape of the neck was significantly higher after 5 days of topical application of acetone-diethylether-water (AEW) compared to pre-treatment levels or to control animals treated with water only. In AEW-treated animals, neurons expressing Fos-like immunoreactivity (FLI) were observed in superficial laminae of the dorsal horn at C3-C5 levels. There was a significant, positive correlation between the number of neurons in lamina I expressing FLI and the number and cumulative duration of spontaneous scratching bouts. These results suggest that this model may be useful to assess mechanisms of dry Skin Pruritus, and that lamina I neurons are activated as a consequence of itching and/or scratching in this model.