The Experts below are selected from a list of 78 Experts worldwide ranked by ideXlab platform
Jiayou Fang - One of the best experts on this subject based on the ideXlab platform.
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Risk assessment of excess drug and sunscreen absorption via Skin with ablative fractional laser resurfacing
Lasers in Medical Science, 2013Co-Authors: Wei Yu Chen, Chia Lang Fang, Hung Hsu Yang, Saleh A. Al-suwayeh, Jiayou FangAbstract:The ablative fractional laser is a new modality used for surgical resurfacing. It is expected that laser treatment can generally deliver drugs into and across the Skin, which is toxicologically relevant. The aim of this study was to establish Skin absorption characteristics of antibiotics, sunscreens, and macromolecules via laser-treated Skin and during postoperative periods. Nude mice were employed as the animal model. The Skin received a single irradiation of a fractional CO_2 laser, using fluences of 4–10 mJ with spot densities of 100–400 spots/cm^2. In vitro Skin permeation using Franz cells was performed. Levels of Skin Water Loss and erythema were evaluated, and histological examinations with staining by hematoxylin and eosin, cyclooxygenase-2, and claudin-1 were carried out. Significant signs of erythema, edema, and scaling of the Skin treated with the fractional laser were evident. Inflammatory infiltration and a reduction in tight junctions were also observed. Laser treatment at 6 mJ increased tetracycline and tretinoin fluxes by 70- and 9-fold, respectively. A higher fluence resulted in a greater tetracycline flux, but lower Skin deposition. On the other hand, tretinoin Skin deposition increased following an increase in the laser fluence. The fractional laser exhibited a negligible effect on modulating oxybenzone absorption. Dextrans with molecular weights of 4 and 10 kDa showed increased fluxes from 0.05 to 11.05 and 38.54 μg/cm^2/h, respectively. The optimized drug dose for Skin treated with the fractional laser was 1/70–1/60 of the regular dose. The Skin histology and drug absorption had recovered to a normal status within 2–3 days. Our findings provide the first report on risk assessment of excessive Skin absorption after fractional laser resurfacing.
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risk assessment of excess drug and sunscreen absorption via Skin with ablative fractional laser resurfacing optimization of the applied dose for postoperative care
Lasers in Medical Science, 2013Co-Authors: Wei Yu Chen, Chia Lang Fang, Saleh A Alsuwayeh, Hung Hsu Yang, Jiayou FangAbstract:The ablative fractional laser is a new modality used for surgical resurfacing. It is expected that laser treatment can generally deliver drugs into and across the Skin, which is toxicologically relevant. The aim of this study was to establish Skin absorption characteristics of antibiotics, sunscreens, and macromolecules via laser-treated Skin and during postoperative periods. Nude mice were employed as the animal model. The Skin received a single irradiation of a fractional CO2 laser, using fluences of 4–10 mJ with spot densities of 100–400 spots/cm2. In vitro Skin permeation using Franz cells was performed. Levels of Skin Water Loss and erythema were evaluated, and histological examinations with staining by hematoxylin and eosin, cyclooxygenase-2, and claudin-1 were carried out. Significant signs of erythema, edema, and scaling of the Skin treated with the fractional laser were evident. Inflammatory infiltration and a reduction in tight junctions were also observed. Laser treatment at 6 mJ increased tetracycline and tretinoin fluxes by 70- and 9-fold, respectively. A higher fluence resulted in a greater tetracycline flux, but lower Skin deposition. On the other hand, tretinoin Skin deposition increased following an increase in the laser fluence. The fractional laser exhibited a negligible effect on modulating oxybenzone absorption. Dextrans with molecular weights of 4 and 10 kDa showed increased fluxes from 0.05 to 11.05 and 38.54 μg/cm2/h, respectively. The optimized drug dose for Skin treated with the fractional laser was 1/70–1/60 of the regular dose. The Skin histology and drug absorption had recovered to a normal status within 2–3 days. Our findings provide the first report on risk assessment of excessive Skin absorption after fractional laser resurfacing.
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Evaluation of drug and sunscreen permeation via Skin irradiated with UVA and UVB: comparisons of normal Skin and chronologically aged Skin.
Journal of dermatological science, 2012Co-Authors: Chi-feng Hung, Chia Lang Fang, Saleh A. Al-suwayeh, Shih Yung Yang, Jiayou FangAbstract:Abstract Background Ultraviolet (UV) exposure is the predominant cause of Skin aging. A systematic evaluation of drug Skin permeation via photoaged Skin is lacking. Objectives The aim of this work was to investigate whether UVA and UVB affect absorption by the Skin of drugs and sunscreens, including tetracycline, quercetin, and oxybenzone. Methods The dorsal Skin of nude mice was subjected to UVA (24 and 39 J/cm 2 ) or UVB (150, 200, and 250 mJ/cm 2 ) irradiation. Levels of Skin Water Loss, erythema, and sebum were evaluated, and histological examinations of COX-2 and claudin-1 expressions were carried out. Permeation of the permeants into and through the Skin was determined in vitro using a Franz cell. In vivo Skin uptake was also evaluated. Senescent Skin (24 weeks old) was used for comparison. Results Wrinkling and scaling were significant signs of Skin treated with UVA and UVB, respectively. The level of claudin-1, an indicator of tight junctions (TJs), was reduced by UVA and UVB irradiation. UVA enhanced tetracycline and quercetin Skin deposition by 11- and 2-fold, respectively. A similar enhancement was shown for flux profiles. Surprisingly, a lower UVA dose revealed greater enhancement compared to the higher dose. The Skin deposition and flux of tetracycline both decreased with UVB exposure. UVB also significantly reduced quercetin flux. The Skin absorption behavior of chronologically aged Skin approximated that of the UVA group, with photoaged Skin showing higher enhancement. UV generally exhibited a negligible effect on modulating oxybenzone permeation. Conclusions Skin disruption produced by UV does not necessarily result in enhanced Skin absorption. It depends on the UV wavelength, irradiated energy, and physicochemical properties of the permeant. To the best of our knowledge, this is the first report establishing drug permeation profiles for UV-irradiated Skin.
Wei Yu Chen - One of the best experts on this subject based on the ideXlab platform.
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Risk assessment of excess drug and sunscreen absorption via Skin with ablative fractional laser resurfacing
Lasers in Medical Science, 2013Co-Authors: Wei Yu Chen, Chia Lang Fang, Hung Hsu Yang, Saleh A. Al-suwayeh, Jiayou FangAbstract:The ablative fractional laser is a new modality used for surgical resurfacing. It is expected that laser treatment can generally deliver drugs into and across the Skin, which is toxicologically relevant. The aim of this study was to establish Skin absorption characteristics of antibiotics, sunscreens, and macromolecules via laser-treated Skin and during postoperative periods. Nude mice were employed as the animal model. The Skin received a single irradiation of a fractional CO_2 laser, using fluences of 4–10 mJ with spot densities of 100–400 spots/cm^2. In vitro Skin permeation using Franz cells was performed. Levels of Skin Water Loss and erythema were evaluated, and histological examinations with staining by hematoxylin and eosin, cyclooxygenase-2, and claudin-1 were carried out. Significant signs of erythema, edema, and scaling of the Skin treated with the fractional laser were evident. Inflammatory infiltration and a reduction in tight junctions were also observed. Laser treatment at 6 mJ increased tetracycline and tretinoin fluxes by 70- and 9-fold, respectively. A higher fluence resulted in a greater tetracycline flux, but lower Skin deposition. On the other hand, tretinoin Skin deposition increased following an increase in the laser fluence. The fractional laser exhibited a negligible effect on modulating oxybenzone absorption. Dextrans with molecular weights of 4 and 10 kDa showed increased fluxes from 0.05 to 11.05 and 38.54 μg/cm^2/h, respectively. The optimized drug dose for Skin treated with the fractional laser was 1/70–1/60 of the regular dose. The Skin histology and drug absorption had recovered to a normal status within 2–3 days. Our findings provide the first report on risk assessment of excessive Skin absorption after fractional laser resurfacing.
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risk assessment of excess drug and sunscreen absorption via Skin with ablative fractional laser resurfacing optimization of the applied dose for postoperative care
Lasers in Medical Science, 2013Co-Authors: Wei Yu Chen, Chia Lang Fang, Saleh A Alsuwayeh, Hung Hsu Yang, Jiayou FangAbstract:The ablative fractional laser is a new modality used for surgical resurfacing. It is expected that laser treatment can generally deliver drugs into and across the Skin, which is toxicologically relevant. The aim of this study was to establish Skin absorption characteristics of antibiotics, sunscreens, and macromolecules via laser-treated Skin and during postoperative periods. Nude mice were employed as the animal model. The Skin received a single irradiation of a fractional CO2 laser, using fluences of 4–10 mJ with spot densities of 100–400 spots/cm2. In vitro Skin permeation using Franz cells was performed. Levels of Skin Water Loss and erythema were evaluated, and histological examinations with staining by hematoxylin and eosin, cyclooxygenase-2, and claudin-1 were carried out. Significant signs of erythema, edema, and scaling of the Skin treated with the fractional laser were evident. Inflammatory infiltration and a reduction in tight junctions were also observed. Laser treatment at 6 mJ increased tetracycline and tretinoin fluxes by 70- and 9-fold, respectively. A higher fluence resulted in a greater tetracycline flux, but lower Skin deposition. On the other hand, tretinoin Skin deposition increased following an increase in the laser fluence. The fractional laser exhibited a negligible effect on modulating oxybenzone absorption. Dextrans with molecular weights of 4 and 10 kDa showed increased fluxes from 0.05 to 11.05 and 38.54 μg/cm2/h, respectively. The optimized drug dose for Skin treated with the fractional laser was 1/70–1/60 of the regular dose. The Skin histology and drug absorption had recovered to a normal status within 2–3 days. Our findings provide the first report on risk assessment of excessive Skin absorption after fractional laser resurfacing.
Chia Lang Fang - One of the best experts on this subject based on the ideXlab platform.
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Risk assessment of excess drug and sunscreen absorption via Skin with ablative fractional laser resurfacing
Lasers in Medical Science, 2013Co-Authors: Wei Yu Chen, Chia Lang Fang, Hung Hsu Yang, Saleh A. Al-suwayeh, Jiayou FangAbstract:The ablative fractional laser is a new modality used for surgical resurfacing. It is expected that laser treatment can generally deliver drugs into and across the Skin, which is toxicologically relevant. The aim of this study was to establish Skin absorption characteristics of antibiotics, sunscreens, and macromolecules via laser-treated Skin and during postoperative periods. Nude mice were employed as the animal model. The Skin received a single irradiation of a fractional CO_2 laser, using fluences of 4–10 mJ with spot densities of 100–400 spots/cm^2. In vitro Skin permeation using Franz cells was performed. Levels of Skin Water Loss and erythema were evaluated, and histological examinations with staining by hematoxylin and eosin, cyclooxygenase-2, and claudin-1 were carried out. Significant signs of erythema, edema, and scaling of the Skin treated with the fractional laser were evident. Inflammatory infiltration and a reduction in tight junctions were also observed. Laser treatment at 6 mJ increased tetracycline and tretinoin fluxes by 70- and 9-fold, respectively. A higher fluence resulted in a greater tetracycline flux, but lower Skin deposition. On the other hand, tretinoin Skin deposition increased following an increase in the laser fluence. The fractional laser exhibited a negligible effect on modulating oxybenzone absorption. Dextrans with molecular weights of 4 and 10 kDa showed increased fluxes from 0.05 to 11.05 and 38.54 μg/cm^2/h, respectively. The optimized drug dose for Skin treated with the fractional laser was 1/70–1/60 of the regular dose. The Skin histology and drug absorption had recovered to a normal status within 2–3 days. Our findings provide the first report on risk assessment of excessive Skin absorption after fractional laser resurfacing.
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risk assessment of excess drug and sunscreen absorption via Skin with ablative fractional laser resurfacing optimization of the applied dose for postoperative care
Lasers in Medical Science, 2013Co-Authors: Wei Yu Chen, Chia Lang Fang, Saleh A Alsuwayeh, Hung Hsu Yang, Jiayou FangAbstract:The ablative fractional laser is a new modality used for surgical resurfacing. It is expected that laser treatment can generally deliver drugs into and across the Skin, which is toxicologically relevant. The aim of this study was to establish Skin absorption characteristics of antibiotics, sunscreens, and macromolecules via laser-treated Skin and during postoperative periods. Nude mice were employed as the animal model. The Skin received a single irradiation of a fractional CO2 laser, using fluences of 4–10 mJ with spot densities of 100–400 spots/cm2. In vitro Skin permeation using Franz cells was performed. Levels of Skin Water Loss and erythema were evaluated, and histological examinations with staining by hematoxylin and eosin, cyclooxygenase-2, and claudin-1 were carried out. Significant signs of erythema, edema, and scaling of the Skin treated with the fractional laser were evident. Inflammatory infiltration and a reduction in tight junctions were also observed. Laser treatment at 6 mJ increased tetracycline and tretinoin fluxes by 70- and 9-fold, respectively. A higher fluence resulted in a greater tetracycline flux, but lower Skin deposition. On the other hand, tretinoin Skin deposition increased following an increase in the laser fluence. The fractional laser exhibited a negligible effect on modulating oxybenzone absorption. Dextrans with molecular weights of 4 and 10 kDa showed increased fluxes from 0.05 to 11.05 and 38.54 μg/cm2/h, respectively. The optimized drug dose for Skin treated with the fractional laser was 1/70–1/60 of the regular dose. The Skin histology and drug absorption had recovered to a normal status within 2–3 days. Our findings provide the first report on risk assessment of excessive Skin absorption after fractional laser resurfacing.
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Evaluation of drug and sunscreen permeation via Skin irradiated with UVA and UVB: comparisons of normal Skin and chronologically aged Skin.
Journal of dermatological science, 2012Co-Authors: Chi-feng Hung, Chia Lang Fang, Saleh A. Al-suwayeh, Shih Yung Yang, Jiayou FangAbstract:Abstract Background Ultraviolet (UV) exposure is the predominant cause of Skin aging. A systematic evaluation of drug Skin permeation via photoaged Skin is lacking. Objectives The aim of this work was to investigate whether UVA and UVB affect absorption by the Skin of drugs and sunscreens, including tetracycline, quercetin, and oxybenzone. Methods The dorsal Skin of nude mice was subjected to UVA (24 and 39 J/cm 2 ) or UVB (150, 200, and 250 mJ/cm 2 ) irradiation. Levels of Skin Water Loss, erythema, and sebum were evaluated, and histological examinations of COX-2 and claudin-1 expressions were carried out. Permeation of the permeants into and through the Skin was determined in vitro using a Franz cell. In vivo Skin uptake was also evaluated. Senescent Skin (24 weeks old) was used for comparison. Results Wrinkling and scaling were significant signs of Skin treated with UVA and UVB, respectively. The level of claudin-1, an indicator of tight junctions (TJs), was reduced by UVA and UVB irradiation. UVA enhanced tetracycline and quercetin Skin deposition by 11- and 2-fold, respectively. A similar enhancement was shown for flux profiles. Surprisingly, a lower UVA dose revealed greater enhancement compared to the higher dose. The Skin deposition and flux of tetracycline both decreased with UVB exposure. UVB also significantly reduced quercetin flux. The Skin absorption behavior of chronologically aged Skin approximated that of the UVA group, with photoaged Skin showing higher enhancement. UV generally exhibited a negligible effect on modulating oxybenzone permeation. Conclusions Skin disruption produced by UV does not necessarily result in enhanced Skin absorption. It depends on the UV wavelength, irradiated energy, and physicochemical properties of the permeant. To the best of our knowledge, this is the first report establishing drug permeation profiles for UV-irradiated Skin.
Hung Hsu Yang - One of the best experts on this subject based on the ideXlab platform.
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Risk assessment of excess drug and sunscreen absorption via Skin with ablative fractional laser resurfacing
Lasers in Medical Science, 2013Co-Authors: Wei Yu Chen, Chia Lang Fang, Hung Hsu Yang, Saleh A. Al-suwayeh, Jiayou FangAbstract:The ablative fractional laser is a new modality used for surgical resurfacing. It is expected that laser treatment can generally deliver drugs into and across the Skin, which is toxicologically relevant. The aim of this study was to establish Skin absorption characteristics of antibiotics, sunscreens, and macromolecules via laser-treated Skin and during postoperative periods. Nude mice were employed as the animal model. The Skin received a single irradiation of a fractional CO_2 laser, using fluences of 4–10 mJ with spot densities of 100–400 spots/cm^2. In vitro Skin permeation using Franz cells was performed. Levels of Skin Water Loss and erythema were evaluated, and histological examinations with staining by hematoxylin and eosin, cyclooxygenase-2, and claudin-1 were carried out. Significant signs of erythema, edema, and scaling of the Skin treated with the fractional laser were evident. Inflammatory infiltration and a reduction in tight junctions were also observed. Laser treatment at 6 mJ increased tetracycline and tretinoin fluxes by 70- and 9-fold, respectively. A higher fluence resulted in a greater tetracycline flux, but lower Skin deposition. On the other hand, tretinoin Skin deposition increased following an increase in the laser fluence. The fractional laser exhibited a negligible effect on modulating oxybenzone absorption. Dextrans with molecular weights of 4 and 10 kDa showed increased fluxes from 0.05 to 11.05 and 38.54 μg/cm^2/h, respectively. The optimized drug dose for Skin treated with the fractional laser was 1/70–1/60 of the regular dose. The Skin histology and drug absorption had recovered to a normal status within 2–3 days. Our findings provide the first report on risk assessment of excessive Skin absorption after fractional laser resurfacing.
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risk assessment of excess drug and sunscreen absorption via Skin with ablative fractional laser resurfacing optimization of the applied dose for postoperative care
Lasers in Medical Science, 2013Co-Authors: Wei Yu Chen, Chia Lang Fang, Saleh A Alsuwayeh, Hung Hsu Yang, Jiayou FangAbstract:The ablative fractional laser is a new modality used for surgical resurfacing. It is expected that laser treatment can generally deliver drugs into and across the Skin, which is toxicologically relevant. The aim of this study was to establish Skin absorption characteristics of antibiotics, sunscreens, and macromolecules via laser-treated Skin and during postoperative periods. Nude mice were employed as the animal model. The Skin received a single irradiation of a fractional CO2 laser, using fluences of 4–10 mJ with spot densities of 100–400 spots/cm2. In vitro Skin permeation using Franz cells was performed. Levels of Skin Water Loss and erythema were evaluated, and histological examinations with staining by hematoxylin and eosin, cyclooxygenase-2, and claudin-1 were carried out. Significant signs of erythema, edema, and scaling of the Skin treated with the fractional laser were evident. Inflammatory infiltration and a reduction in tight junctions were also observed. Laser treatment at 6 mJ increased tetracycline and tretinoin fluxes by 70- and 9-fold, respectively. A higher fluence resulted in a greater tetracycline flux, but lower Skin deposition. On the other hand, tretinoin Skin deposition increased following an increase in the laser fluence. The fractional laser exhibited a negligible effect on modulating oxybenzone absorption. Dextrans with molecular weights of 4 and 10 kDa showed increased fluxes from 0.05 to 11.05 and 38.54 μg/cm2/h, respectively. The optimized drug dose for Skin treated with the fractional laser was 1/70–1/60 of the regular dose. The Skin histology and drug absorption had recovered to a normal status within 2–3 days. Our findings provide the first report on risk assessment of excessive Skin absorption after fractional laser resurfacing.
Saleh A. Al-suwayeh - One of the best experts on this subject based on the ideXlab platform.
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Risk assessment of excess drug and sunscreen absorption via Skin with ablative fractional laser resurfacing
Lasers in Medical Science, 2013Co-Authors: Wei Yu Chen, Chia Lang Fang, Hung Hsu Yang, Saleh A. Al-suwayeh, Jiayou FangAbstract:The ablative fractional laser is a new modality used for surgical resurfacing. It is expected that laser treatment can generally deliver drugs into and across the Skin, which is toxicologically relevant. The aim of this study was to establish Skin absorption characteristics of antibiotics, sunscreens, and macromolecules via laser-treated Skin and during postoperative periods. Nude mice were employed as the animal model. The Skin received a single irradiation of a fractional CO_2 laser, using fluences of 4–10 mJ with spot densities of 100–400 spots/cm^2. In vitro Skin permeation using Franz cells was performed. Levels of Skin Water Loss and erythema were evaluated, and histological examinations with staining by hematoxylin and eosin, cyclooxygenase-2, and claudin-1 were carried out. Significant signs of erythema, edema, and scaling of the Skin treated with the fractional laser were evident. Inflammatory infiltration and a reduction in tight junctions were also observed. Laser treatment at 6 mJ increased tetracycline and tretinoin fluxes by 70- and 9-fold, respectively. A higher fluence resulted in a greater tetracycline flux, but lower Skin deposition. On the other hand, tretinoin Skin deposition increased following an increase in the laser fluence. The fractional laser exhibited a negligible effect on modulating oxybenzone absorption. Dextrans with molecular weights of 4 and 10 kDa showed increased fluxes from 0.05 to 11.05 and 38.54 μg/cm^2/h, respectively. The optimized drug dose for Skin treated with the fractional laser was 1/70–1/60 of the regular dose. The Skin histology and drug absorption had recovered to a normal status within 2–3 days. Our findings provide the first report on risk assessment of excessive Skin absorption after fractional laser resurfacing.
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Evaluation of drug and sunscreen permeation via Skin irradiated with UVA and UVB: comparisons of normal Skin and chronologically aged Skin.
Journal of dermatological science, 2012Co-Authors: Chi-feng Hung, Chia Lang Fang, Saleh A. Al-suwayeh, Shih Yung Yang, Jiayou FangAbstract:Abstract Background Ultraviolet (UV) exposure is the predominant cause of Skin aging. A systematic evaluation of drug Skin permeation via photoaged Skin is lacking. Objectives The aim of this work was to investigate whether UVA and UVB affect absorption by the Skin of drugs and sunscreens, including tetracycline, quercetin, and oxybenzone. Methods The dorsal Skin of nude mice was subjected to UVA (24 and 39 J/cm 2 ) or UVB (150, 200, and 250 mJ/cm 2 ) irradiation. Levels of Skin Water Loss, erythema, and sebum were evaluated, and histological examinations of COX-2 and claudin-1 expressions were carried out. Permeation of the permeants into and through the Skin was determined in vitro using a Franz cell. In vivo Skin uptake was also evaluated. Senescent Skin (24 weeks old) was used for comparison. Results Wrinkling and scaling were significant signs of Skin treated with UVA and UVB, respectively. The level of claudin-1, an indicator of tight junctions (TJs), was reduced by UVA and UVB irradiation. UVA enhanced tetracycline and quercetin Skin deposition by 11- and 2-fold, respectively. A similar enhancement was shown for flux profiles. Surprisingly, a lower UVA dose revealed greater enhancement compared to the higher dose. The Skin deposition and flux of tetracycline both decreased with UVB exposure. UVB also significantly reduced quercetin flux. The Skin absorption behavior of chronologically aged Skin approximated that of the UVA group, with photoaged Skin showing higher enhancement. UV generally exhibited a negligible effect on modulating oxybenzone permeation. Conclusions Skin disruption produced by UV does not necessarily result in enhanced Skin absorption. It depends on the UV wavelength, irradiated energy, and physicochemical properties of the permeant. To the best of our knowledge, this is the first report establishing drug permeation profiles for UV-irradiated Skin.