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I. David Goldman - One of the best experts on this subject based on the ideXlab platform.
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Substituted-cysteine accessibility and cross-linking identify an exofacial cleft in the 7th and 8th helices of the proton-coupled folate transporter (SLC46A1)
American Journal of Physiology-cell Physiology, 2017Co-Authors: Srinivas Aluri, Rongbao Zhao, Andras Fiser, I. David GoldmanAbstract:The proton-coupled folate transporter (PCFT-SLC46A1) is required for folate transport across the apical membrane of the small intestine and across the choroid plexus. This study focuses on the stru...
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A novel deletion mutation in the proton-coupled folate transporter (PCFT; SLC46A1) in a Nicaraguan child with hereditary folate malabsorption.
Gene, 2013Co-Authors: Ndeye Diop-bove, Fernando Scaglia, M. Jain, I. David GoldmanAbstract:Abstract Hereditary folate malabsorption (OMIM 229050 ) is a rare autosomal recessive disorder caused by loss-of-function mutations in the proton-coupled folate transporter gene (pcft/SLC46A1) resulting in impaired folate transport across the intestine and into the central nervous system. We report a novel, homozygous, deletion mutation in a child of Nicaraguan descent in exon 2 (c.558–588 del, ss778190447) at amino acid position I188 resulting in a frameshift with a premature stop.
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Role of the fourth transmembrane domain in proton-coupled folate transporter function as assessed by the substituted cysteine accessibility method
American Journal of Physiology-cell Physiology, 2013Co-Authors: Daniel Sanghoon Shin, Rongbao Zhao, Andras Fiser, I. David GoldmanAbstract:The proton-coupled folate transporter (PCFT, SLC46A1) mediates folate transport across the apical brush-border membrane of the proximal small intestine and the basolateral membrane of choroid plexu...
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Folate and thiamine transporters mediated by facilitative carriers (SLC19A1-3 and SLC46A1) and folate receptors.
Molecular aspects of medicine, 2013Co-Authors: Rongbao Zhao, I. David GoldmanAbstract:The reduced folate carrier (RFC, SLC19A1), thiamine transporter-1 (ThTr1, SLC19A2) and thiamine transporter-2 (ThTr2, SLC19A3) evolved from the same family of solute carriers. SLC19A1 transports folates but not thiamine. SLC19A2 and SLC19A3 transport thiamine but not folates. SLC19A1 and SLC19A2 deliver their substrates to systemic tissues; SLC19A3 mediates intestinal thiamine absorption. The proton-coupled folate transporter (PCFT, SLC46A1) is the mechanism by which folates are absorbed across the apical-brush-border membrane of the proximal small intestine. Two folate receptors (FOLR1 and FOLR2) mediate folate transport across epithelia by an endocytic process. Folate transporters are routes of delivery of drugs for the treatment of cancer and inflammatory diseases. There are autosomal recessive disorders associated with mutations in genes encoded for SLC46A1 (hereditary folate malabsorption), FOLR1 (cerebral folate deficiency), SLC19A2 (thiamine-responsive megaloblastic anemia), and SLC19A3 (biotin-responsive basal ganglia disease).
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Properties of the Arg376 residue of the proton-coupled folate transporter (PCFT-SLC46A1) and a glutamine mutant causing hereditary folate malabsorption.
American journal of physiology. Cell physiology, 2010Co-Authors: Kris Michael Mahadeo, Rongbao Zhao, Ersin Selcuk Unal, Ndeye Diop-bove, Daniel Shin, Juliana Teo, Min Hwang Chang, Andreas Fulterer, Michael F. Romero, I. David GoldmanAbstract:The proton-coupled folate transporter (PCFT-SLC46A1) is required for intestinal folate absorption and is mutated in the autosomal recessive disorder, hereditary folate malabsorption (HFM). This rep...
Rongbao Zhao - One of the best experts on this subject based on the ideXlab platform.
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Substituted-cysteine accessibility and cross-linking identify an exofacial cleft in the 7th and 8th helices of the proton-coupled folate transporter (SLC46A1)
American Journal of Physiology-cell Physiology, 2017Co-Authors: Srinivas Aluri, Rongbao Zhao, Andras Fiser, I. David GoldmanAbstract:The proton-coupled folate transporter (PCFT-SLC46A1) is required for folate transport across the apical membrane of the small intestine and across the choroid plexus. This study focuses on the stru...
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Role of the fourth transmembrane domain in proton-coupled folate transporter function as assessed by the substituted cysteine accessibility method
American Journal of Physiology-cell Physiology, 2013Co-Authors: Daniel Sanghoon Shin, Rongbao Zhao, Andras Fiser, I. David GoldmanAbstract:The proton-coupled folate transporter (PCFT, SLC46A1) mediates folate transport across the apical brush-border membrane of the proximal small intestine and the basolateral membrane of choroid plexu...
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Folate and thiamine transporters mediated by facilitative carriers (SLC19A1-3 and SLC46A1) and folate receptors.
Molecular aspects of medicine, 2013Co-Authors: Rongbao Zhao, I. David GoldmanAbstract:The reduced folate carrier (RFC, SLC19A1), thiamine transporter-1 (ThTr1, SLC19A2) and thiamine transporter-2 (ThTr2, SLC19A3) evolved from the same family of solute carriers. SLC19A1 transports folates but not thiamine. SLC19A2 and SLC19A3 transport thiamine but not folates. SLC19A1 and SLC19A2 deliver their substrates to systemic tissues; SLC19A3 mediates intestinal thiamine absorption. The proton-coupled folate transporter (PCFT, SLC46A1) is the mechanism by which folates are absorbed across the apical-brush-border membrane of the proximal small intestine. Two folate receptors (FOLR1 and FOLR2) mediate folate transport across epithelia by an endocytic process. Folate transporters are routes of delivery of drugs for the treatment of cancer and inflammatory diseases. There are autosomal recessive disorders associated with mutations in genes encoded for SLC46A1 (hereditary folate malabsorption), FOLR1 (cerebral folate deficiency), SLC19A2 (thiamine-responsive megaloblastic anemia), and SLC19A3 (biotin-responsive basal ganglia disease).
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Functional roles of the A335 and G338 residues of the proton-coupled folate transporter (PCFT-SLC46A1) mutated in hereditary folate malabsorption.
American Journal of Physiology-cell Physiology, 2012Co-Authors: Daniel Sanghoon Shin, Rongbao Zhao, Andras Fiser, David I GoldmanAbstract:The proton-coupled folate transporter (PCFT-SLC46A1) mediates intestinal folate absorption and folate transport across the choroid plexus, processes defective in hereditary folate malabsorption (HF...
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Properties of the Arg376 residue of the proton-coupled folate transporter (PCFT-SLC46A1) and a glutamine mutant causing hereditary folate malabsorption.
American journal of physiology. Cell physiology, 2010Co-Authors: Kris Michael Mahadeo, Rongbao Zhao, Ersin Selcuk Unal, Ndeye Diop-bove, Daniel Shin, Juliana Teo, Min Hwang Chang, Andreas Fulterer, Michael F. Romero, I. David GoldmanAbstract:The proton-coupled folate transporter (PCFT-SLC46A1) is required for intestinal folate absorption and is mutated in the autosomal recessive disorder, hereditary folate malabsorption (HFM). This rep...
Yoshinori Moriyama - One of the best experts on this subject based on the ideXlab platform.
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type 1 sodium dependent phosphate transporter slc17a1 protein is a cl dependent urate exporter
Journal of Biological Chemistry, 2010Co-Authors: Masafumi Iharada, Takaaki Miyaji, Hiroshi Omote, Takahiro Fujimoto, Miki Hiasa, Yoshinori MoriyamaAbstract:Abstract SLC17A1 protein (NPT1) is the first identified member of the SLC17 phosphate transporter family and mediates the transmembrane cotransport of Na+/Pi in oocytes. Although this protein is believed to be a renal polyspecific anion exporter, its transport properties are not well characterized. Here, we show that proteoliposomes containing purified SLC17A1 transport various organic anions such as p-aminohippuric acid and acetylsalicylic acid (aspirin) in an inside positive membrane potential (Δψ)-dependent manner. We found that NPT1 also transported urate. The uptake characteristics were similar to that of SLC17 members in its Cl− dependence and inhibitor sensitivity. When arginine 138, an essential amino acid residue for members of the SLC17 family such as the vesicular glutamate transporter, was specifically mutated to alanine, the resulting mutant protein was inactive in Δψ-dependent anion transport. Heterologously expressed and purified human NPT1 carrying the single nucleotide polymorphism mutation that is associated with increased risk of gout in humans exhibited 32% lower urate transport activity compared with the wild type protein. These results strongly suggested that NPT1 is a Cl−-dependent polyspecific anion exporter involved in urate excretion under physiological conditions.
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type 1 sodium dependent phosphate transporter slc17a1 protein is a cl dependent urate exporter
Journal of Biological Chemistry, 2010Co-Authors: Masafumi Iharada, Takaaki Miyaji, Hiroshi Omote, Takahiro Fujimoto, Miki Hiasa, Yoshinori MoriyamaAbstract:SLC17A1 protein (NPT1) is the first identified member of the SLC17 phosphate transporter family and mediates the transmembrane cotransport of Na(+)/P(i) in oocytes. Although this protein is believed to be a renal polyspecific anion exporter, its transport properties are not well characterized. Here, we show that proteoliposomes containing purified SLC17A1 transport various organic anions such as p-aminohippuric acid and acetylsalicylic acid (aspirin) in an inside positive membrane potential (Deltapsi)-dependent manner. We found that NPT1 also transported urate. The uptake characteristics were similar to that of SLC17 members in its Cl(-) dependence and inhibitor sensitivity. When arginine 138, an essential amino acid residue for members of the SLC17 family such as the vesicular glutamate transporter, was specifically mutated to alanine, the resulting mutant protein was inactive in Deltapsi-dependent anion transport. Heterologously expressed and purified human NPT1 carrying the single nucleotide polymorphism mutation that is associated with increased risk of gout in humans exhibited 32% lower urate transport activity compared with the wild type protein. These results strongly suggested that NPT1 is a Cl(-)-dependent polyspecific anion exporter involved in urate excretion under physiological conditions.
Andras Fiser - One of the best experts on this subject based on the ideXlab platform.
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Substituted-cysteine accessibility and cross-linking identify an exofacial cleft in the 7th and 8th helices of the proton-coupled folate transporter (SLC46A1)
American Journal of Physiology-cell Physiology, 2017Co-Authors: Srinivas Aluri, Rongbao Zhao, Andras Fiser, I. David GoldmanAbstract:The proton-coupled folate transporter (PCFT-SLC46A1) is required for folate transport across the apical membrane of the small intestine and across the choroid plexus. This study focuses on the stru...
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Role of the fourth transmembrane domain in proton-coupled folate transporter function as assessed by the substituted cysteine accessibility method
American Journal of Physiology-cell Physiology, 2013Co-Authors: Daniel Sanghoon Shin, Rongbao Zhao, Andras Fiser, I. David GoldmanAbstract:The proton-coupled folate transporter (PCFT, SLC46A1) mediates folate transport across the apical brush-border membrane of the proximal small intestine and the basolateral membrane of choroid plexu...
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Functional roles of the A335 and G338 residues of the proton-coupled folate transporter (PCFT-SLC46A1) mutated in hereditary folate malabsorption.
American Journal of Physiology-cell Physiology, 2012Co-Authors: Daniel Sanghoon Shin, Rongbao Zhao, Andras Fiser, David I GoldmanAbstract:The proton-coupled folate transporter (PCFT-SLC46A1) mediates intestinal folate absorption and folate transport across the choroid plexus, processes defective in hereditary folate malabsorption (HF...
Daniel Sanghoon Shin - One of the best experts on this subject based on the ideXlab platform.
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Role of the fourth transmembrane domain in proton-coupled folate transporter function as assessed by the substituted cysteine accessibility method
American Journal of Physiology-cell Physiology, 2013Co-Authors: Daniel Sanghoon Shin, Rongbao Zhao, Andras Fiser, I. David GoldmanAbstract:The proton-coupled folate transporter (PCFT, SLC46A1) mediates folate transport across the apical brush-border membrane of the proximal small intestine and the basolateral membrane of choroid plexu...
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Functional roles of the A335 and G338 residues of the proton-coupled folate transporter (PCFT-SLC46A1) mutated in hereditary folate malabsorption.
American Journal of Physiology-cell Physiology, 2012Co-Authors: Daniel Sanghoon Shin, Rongbao Zhao, Andras Fiser, David I GoldmanAbstract:The proton-coupled folate transporter (PCFT-SLC46A1) mediates intestinal folate absorption and folate transport across the choroid plexus, processes defective in hereditary folate malabsorption (HF...