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Robert Hnasko - One of the best experts on this subject based on the ideXlab platform.
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Chemical Characterization of Polysaccharide from the Slime Layer of the Cyanobacterium Microcystis flos-aquae C3-40.
Applied and Environmental Microbiology, 1991Co-Authors: John L. Plude, Dorothy L. Parker, Olivia J. Schommer, Robert J. Timmerman, Stephanie A. Hagstrom, James M. Joers, Robert HnaskoAbstract:Abstract Macromolecular material from the Slime Layer of the cyanobacterium Microcystis flos-aquae C3-40 was defined as material that adhered to cells during centrifugation in growth medium but was dislodged by washing with deionized water and retained within dialysis tubing with a molecular-weight cutoff of 3,500. At each step of this isolation procedure, the Slime was observed microscopically. Cells in the centrifugal pellet were surrounded by large amounts of Slime that excluded negative stain, whereas cells that had been washed with water lacked visible Slime. Two independently isolated lots of Slime contained no detectable protein (
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chemical characterization of polysaccharide from the Slime Layer of the cyanobacterium microcystis flos aquae c3 40
Applied and Environmental Microbiology, 1991Co-Authors: John L. Plude, Dorothy L. Parker, Olivia J. Schommer, Robert J. Timmerman, Stephanie A. Hagstrom, James M. Joers, Robert HnaskoAbstract:Abstract Macromolecular material from the Slime Layer of the cyanobacterium Microcystis flos-aquae C3-40 was defined as material that adhered to cells during centrifugation in growth medium but was dislodged by washing with deionized water and retained within dialysis tubing with a molecular-weight cutoff of 3,500. At each step of this isolation procedure, the Slime was observed microscopically. Cells in the centrifugal pellet were surrounded by large amounts of Slime that excluded negative stain, whereas cells that had been washed with water lacked visible Slime. Two independently isolated lots of Slime contained no detectable protein (<1%, wt/wt) and consisted predominantly of anthrone-reacting polysaccharide. Sugars in a hydrolysate of Slime polysaccharide were derivatized with trimethylsilylimidazole and examined by gas chromatography-mass spectrometry. The composition of the Slime polysaccharide was 1.5% (wt/wt) galactose, 2.0% glucose, 3.0% xylose, 5.0% mannose, 5.5% rhamnose, and 83% galacturonic acid. This composition resembles that of the plant polysaccharide pectin, which was treated in parallel as a control. Consistent with earlier indications that M. flos-aquae Slime preferentially binds certain cations, the ratio of Fe to Na in the dialyzed Slime was 104 times that in the growth medium. The composition of the Slime is discussed with respect to possible mechanisms of cation binding in comparison with other cyanobacterial exopolysaccharides and pectin.
John L. Plude - One of the best experts on this subject based on the ideXlab platform.
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microcystis flos aquae c3 40 from the Slime Layer of the cyanobacterium chemical characterization of polysaccharide
2013Co-Authors: Stephanie A. Hagstrom, John L. Plude, Dorothy L. Parker, James M. Joers, Olivia J. SchommerAbstract:number of chemicaland ecological properties of freshwater ecosystems. Forexample, the Slime from M.flos-aquae and related organ-ismshasbeenshownto interact strongly withcations (7, 19,21) and to be involved in oxidative precipitation ofmanga-nese nodules in certain lakes (25). Suchadsorption ofmetalcations can influence the availability ofseveral trace nutri-ents, sequester toxic metals,
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Chemical Characterization of Polysaccharide from the Slime Layer of the Cyanobacterium Microcystis flos-aquae C3-40.
Applied and Environmental Microbiology, 1991Co-Authors: John L. Plude, Dorothy L. Parker, Olivia J. Schommer, Robert J. Timmerman, Stephanie A. Hagstrom, James M. Joers, Robert HnaskoAbstract:Abstract Macromolecular material from the Slime Layer of the cyanobacterium Microcystis flos-aquae C3-40 was defined as material that adhered to cells during centrifugation in growth medium but was dislodged by washing with deionized water and retained within dialysis tubing with a molecular-weight cutoff of 3,500. At each step of this isolation procedure, the Slime was observed microscopically. Cells in the centrifugal pellet were surrounded by large amounts of Slime that excluded negative stain, whereas cells that had been washed with water lacked visible Slime. Two independently isolated lots of Slime contained no detectable protein (
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chemical characterization of polysaccharide from the Slime Layer of the cyanobacterium microcystis flos aquae c3 40
Applied and Environmental Microbiology, 1991Co-Authors: John L. Plude, Dorothy L. Parker, Olivia J. Schommer, Robert J. Timmerman, Stephanie A. Hagstrom, James M. Joers, Robert HnaskoAbstract:Abstract Macromolecular material from the Slime Layer of the cyanobacterium Microcystis flos-aquae C3-40 was defined as material that adhered to cells during centrifugation in growth medium but was dislodged by washing with deionized water and retained within dialysis tubing with a molecular-weight cutoff of 3,500. At each step of this isolation procedure, the Slime was observed microscopically. Cells in the centrifugal pellet were surrounded by large amounts of Slime that excluded negative stain, whereas cells that had been washed with water lacked visible Slime. Two independently isolated lots of Slime contained no detectable protein (<1%, wt/wt) and consisted predominantly of anthrone-reacting polysaccharide. Sugars in a hydrolysate of Slime polysaccharide were derivatized with trimethylsilylimidazole and examined by gas chromatography-mass spectrometry. The composition of the Slime polysaccharide was 1.5% (wt/wt) galactose, 2.0% glucose, 3.0% xylose, 5.0% mannose, 5.5% rhamnose, and 83% galacturonic acid. This composition resembles that of the plant polysaccharide pectin, which was treated in parallel as a control. Consistent with earlier indications that M. flos-aquae Slime preferentially binds certain cations, the ratio of Fe to Na in the dialyzed Slime was 104 times that in the growth medium. The composition of the Slime is discussed with respect to possible mechanisms of cation binding in comparison with other cyanobacterial exopolysaccharides and pectin.
Evangelos D Anastassiou - One of the best experts on this subject based on the ideXlab platform.
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identity of macromolecules present in the extracellular Slime Layer of staphylococcus epidermidis
Biochimie, 1995Co-Authors: Nikos K Karamanos, H S Panagiotopoulou, Alexandra Syrokou, C Frangides, Anders Hjerpe, G Dimitracopoulos, Evangelos D AnastassiouAbstract:Abstract The extracellular Slime Layer of Staphylococcus epidermidis ATCC 35983 contains: a) two non-anionic carbohydrate containing proteins degradable with papain of molecular masses 250 and 125 kDa; b) a polydisperse but homogeneously-charged acidic population with Mr, ranging from 120 000 to 35 000 (average Mr 80 000) containing a polysaccharide covalently bound to a small peptide; c) a papain degradable macromolecule (molecular mass 60 kDa) bearing acidic carbohydrates covalently bound to protein; and c) two acidic polysaccharides strongly retained by the anion-exchange column; one polyssacharide is sulphated and has a molecular mass of 20 kDa; the other has a higher charge density and a molecular mass of 12.5 kDa. The results obtained clearly demonstrate the presence of discrete macromolecules in the extracellular material of Slime-producing S epidermidis, the majority of which contain acidic carbohydrates, whose biological role remains to be elucidated.
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isolation and characterization of a novel 20 kda sulfated polysaccharide from the extracellular Slime Layer of staphylococcus epidermidis
Archives of Biochemistry and Biophysics, 1994Co-Authors: A Arvaniti, Nikos K Karamanos, G Dimitracopoulos, Evangelos D AnastassiouAbstract:Slime-producing coagulase-negative staphylococci have emerged as important pathogens especially in immunocompromised hosts and patients with implanted devices. Although the extracellular Slime Layer is considered an important virulence factor, the chemical composition of the Slime polysaccharide(s) remains unknown. The crude Slime product derived from two reference Staphylococcus epidermidis strains (ATCC 35983 and 35984) and two clinical isolates was found to contain protein (11-20.5%), hexosamines (8-19%), neutral sugars (12.2-14%), phosphates (4-9.5%), uronic acids (1-13%), and small amounts of sulfates (0.5-3%). Preparative anion-exchange chromatography separated a main carbohydrate component which was isolated by combined chromatographies on DEAE-Sephacel and Sepharose CL-6B. HPLC and electrophoreses on polyacrylamide gel and cellulose acetate membrane revealed the presence of one species of low-sulfated polysaccharide with a relative molecular mass of 20-kDa. Chemical analyses of the polysaccharide showed that it is rich in glucosamine (46%) and neutral sugars (30-34%) with small amounts of sulfates (5.7-6.5%) and glucuronic acid (2.9-3.4%). Ten percent of the glucosamine is sulfated at the amino group. The neutral monosaccharides present are glucose, fucose, and xylose with glucose as the predominant one. It is estimated that the polysaccharide consists of 61-65 molecules of glucosamine (6-7 of which are N-sulfated), 30-35 neutral monosaccharides, 3-4 molecules of glucuronic acid, and 1-3 of fucose and xylose. Isolation and characterization of such a polysaccharide from the extracellular Slime Layer of S. epidermidis has not been previously reported. Its role to pathogenicity remains to be elucidated.
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occurrence of a 29 kda polysaccharide in the Slime Layer of both smooth and rough strains of pseudomonas aeruginosa
International Journal of Biochemistry, 1993Co-Authors: Myrto Christofidou, G Dimitracopoulos, N K Karamanos, Anastassios C Mintzas, Evangelos D AnastassiouAbstract:1. 1. The lipopolysaccharide (LPS) and the extracellular products (Slime) of a smooth, nonmucoid Pseudomonas aeruginosa strain (PAC IR) and its rough mutant (PAC 605) were subjected to a comparative biochemical analysis. 2. 2. Chemical and electrophoretic analyses suggested that the Slime preparation of both strains are composed mainly of similar carbohydrate components which are different from those of the respective lipopolysaccharides. 3. 3. Chromatographie analysis of the two Slime preparations on gel permeation HPLC columns revealed the presence of a major polysaccharide in both strains with an apparent molecular weight 29 kDa and a minor high molecular weight polysaccharide in the PAC IR strain.
Olivia J. Schommer - One of the best experts on this subject based on the ideXlab platform.
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microcystis flos aquae c3 40 from the Slime Layer of the cyanobacterium chemical characterization of polysaccharide
2013Co-Authors: Stephanie A. Hagstrom, John L. Plude, Dorothy L. Parker, James M. Joers, Olivia J. SchommerAbstract:number of chemicaland ecological properties of freshwater ecosystems. Forexample, the Slime from M.flos-aquae and related organ-ismshasbeenshownto interact strongly withcations (7, 19,21) and to be involved in oxidative precipitation ofmanga-nese nodules in certain lakes (25). Suchadsorption ofmetalcations can influence the availability ofseveral trace nutri-ents, sequester toxic metals,
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Chemical Characterization of Polysaccharide from the Slime Layer of the Cyanobacterium Microcystis flos-aquae C3-40.
Applied and Environmental Microbiology, 1991Co-Authors: John L. Plude, Dorothy L. Parker, Olivia J. Schommer, Robert J. Timmerman, Stephanie A. Hagstrom, James M. Joers, Robert HnaskoAbstract:Abstract Macromolecular material from the Slime Layer of the cyanobacterium Microcystis flos-aquae C3-40 was defined as material that adhered to cells during centrifugation in growth medium but was dislodged by washing with deionized water and retained within dialysis tubing with a molecular-weight cutoff of 3,500. At each step of this isolation procedure, the Slime was observed microscopically. Cells in the centrifugal pellet were surrounded by large amounts of Slime that excluded negative stain, whereas cells that had been washed with water lacked visible Slime. Two independently isolated lots of Slime contained no detectable protein (
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chemical characterization of polysaccharide from the Slime Layer of the cyanobacterium microcystis flos aquae c3 40
Applied and Environmental Microbiology, 1991Co-Authors: John L. Plude, Dorothy L. Parker, Olivia J. Schommer, Robert J. Timmerman, Stephanie A. Hagstrom, James M. Joers, Robert HnaskoAbstract:Abstract Macromolecular material from the Slime Layer of the cyanobacterium Microcystis flos-aquae C3-40 was defined as material that adhered to cells during centrifugation in growth medium but was dislodged by washing with deionized water and retained within dialysis tubing with a molecular-weight cutoff of 3,500. At each step of this isolation procedure, the Slime was observed microscopically. Cells in the centrifugal pellet were surrounded by large amounts of Slime that excluded negative stain, whereas cells that had been washed with water lacked visible Slime. Two independently isolated lots of Slime contained no detectable protein (<1%, wt/wt) and consisted predominantly of anthrone-reacting polysaccharide. Sugars in a hydrolysate of Slime polysaccharide were derivatized with trimethylsilylimidazole and examined by gas chromatography-mass spectrometry. The composition of the Slime polysaccharide was 1.5% (wt/wt) galactose, 2.0% glucose, 3.0% xylose, 5.0% mannose, 5.5% rhamnose, and 83% galacturonic acid. This composition resembles that of the plant polysaccharide pectin, which was treated in parallel as a control. Consistent with earlier indications that M. flos-aquae Slime preferentially binds certain cations, the ratio of Fe to Na in the dialyzed Slime was 104 times that in the growth medium. The composition of the Slime is discussed with respect to possible mechanisms of cation binding in comparison with other cyanobacterial exopolysaccharides and pectin.
Stephanie A. Hagstrom - One of the best experts on this subject based on the ideXlab platform.
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microcystis flos aquae c3 40 from the Slime Layer of the cyanobacterium chemical characterization of polysaccharide
2013Co-Authors: Stephanie A. Hagstrom, John L. Plude, Dorothy L. Parker, James M. Joers, Olivia J. SchommerAbstract:number of chemicaland ecological properties of freshwater ecosystems. Forexample, the Slime from M.flos-aquae and related organ-ismshasbeenshownto interact strongly withcations (7, 19,21) and to be involved in oxidative precipitation ofmanga-nese nodules in certain lakes (25). Suchadsorption ofmetalcations can influence the availability ofseveral trace nutri-ents, sequester toxic metals,
-
Chemical Characterization of Polysaccharide from the Slime Layer of the Cyanobacterium Microcystis flos-aquae C3-40.
Applied and Environmental Microbiology, 1991Co-Authors: John L. Plude, Dorothy L. Parker, Olivia J. Schommer, Robert J. Timmerman, Stephanie A. Hagstrom, James M. Joers, Robert HnaskoAbstract:Abstract Macromolecular material from the Slime Layer of the cyanobacterium Microcystis flos-aquae C3-40 was defined as material that adhered to cells during centrifugation in growth medium but was dislodged by washing with deionized water and retained within dialysis tubing with a molecular-weight cutoff of 3,500. At each step of this isolation procedure, the Slime was observed microscopically. Cells in the centrifugal pellet were surrounded by large amounts of Slime that excluded negative stain, whereas cells that had been washed with water lacked visible Slime. Two independently isolated lots of Slime contained no detectable protein (
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chemical characterization of polysaccharide from the Slime Layer of the cyanobacterium microcystis flos aquae c3 40
Applied and Environmental Microbiology, 1991Co-Authors: John L. Plude, Dorothy L. Parker, Olivia J. Schommer, Robert J. Timmerman, Stephanie A. Hagstrom, James M. Joers, Robert HnaskoAbstract:Abstract Macromolecular material from the Slime Layer of the cyanobacterium Microcystis flos-aquae C3-40 was defined as material that adhered to cells during centrifugation in growth medium but was dislodged by washing with deionized water and retained within dialysis tubing with a molecular-weight cutoff of 3,500. At each step of this isolation procedure, the Slime was observed microscopically. Cells in the centrifugal pellet were surrounded by large amounts of Slime that excluded negative stain, whereas cells that had been washed with water lacked visible Slime. Two independently isolated lots of Slime contained no detectable protein (<1%, wt/wt) and consisted predominantly of anthrone-reacting polysaccharide. Sugars in a hydrolysate of Slime polysaccharide were derivatized with trimethylsilylimidazole and examined by gas chromatography-mass spectrometry. The composition of the Slime polysaccharide was 1.5% (wt/wt) galactose, 2.0% glucose, 3.0% xylose, 5.0% mannose, 5.5% rhamnose, and 83% galacturonic acid. This composition resembles that of the plant polysaccharide pectin, which was treated in parallel as a control. Consistent with earlier indications that M. flos-aquae Slime preferentially binds certain cations, the ratio of Fe to Na in the dialyzed Slime was 104 times that in the growth medium. The composition of the Slime is discussed with respect to possible mechanisms of cation binding in comparison with other cyanobacterial exopolysaccharides and pectin.