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David Canoterriza - One of the best experts on this subject based on the ideXlab platform.
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epidemiological surveillance of schmallenberg virus in Small Ruminants in southern spain
Transboundary and Emerging Diseases, 2020Co-Authors: Debora Jimenezmartin, David Canoterriza, Jose M Diazcao, Joan Pujols, Manuel Fernandezmorente, Ignacio GarciabocanegraAbstract:Schmallenberg virus (SBV) is an emerging Culicoides-borne Orthobunyavirus that affects ruminant species. Between 2011 and 2013, it was responsible for a large-scale epidemic in Europe. In the present study, we aimed to determine the seroprevalence, spatial distribution and risk factors associated with SBV exposure in sheep and goats in the region where the first Schmallenberg disease outbreak in Spain was reported. Blood samples from 1,796 Small Ruminants from 120 farms were collected in Andalusia (southern Spain) between 2015 and 2017. Antibodies against SBV were detected in 536 of 1,796 animals (29.8%; 95%CI: 27.7-32.0) using a commercial blocking ELISA. The individual seroprevalence according to species was 31.1% (280/900; 95%CI: 28.1-34.1) in sheep and 28.6% (256/896; 95%CI: 25.6-31.5) in goats. The farm prevalence was 76.7% (95%CI: 69.1-84.2). Seropositivity to SBV was confirmed in both sheep and goats in all provinces by virus neutralization test. Two significant (p 14oC) and altitude (<400 metres above sea level) were risk factors associated with SBV exposure in Small Ruminants. Our results highlight widespread but not homogeneous circulation of SBV in Small ruminant populations in Spain.
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epidemiological surveillance of toxoplasma gondii in Small Ruminants in southern spain
Preventive Veterinary Medicine, 2020Co-Authors: Debora Jimenezmartin, Ignacio Garciabocanegra, S Almeria, Sabrina Castroscholten, J P Dubey, M A Amarolopez, David CanoterrizaAbstract:Toxoplasmosis, caused by the protozoan Toxoplasma gondii, is a worldwide zoonotic disease, which affects most warm-blooded species. Besides the zoonotic relevance, toxoplasmosis is one of the major causes of reproductive disorders in Small Ruminants. A cross-sectional study was carried out to determine the seroprevalence, spatial distribution and risk factors associated with T. gondii infection in sheep and goats in southern Spain. During 2015-2017, a total of 1,943 Small Ruminants (998 sheep and 945 goats) from 127 flocks were tested for antibodies against T. gondii using the modified agglutination test (MAT, cut-off 1:25). Antibodies against T. gondii were detected in 464 of the 998 sheep (46.5 %; CI95 %: 43.4-49.6%) and 362 of the 945 goats (38.3 %; CI95 %: 35.2-41.4%) tested. The farm prevalence was 98.4 % (CI95 %: 95.4-100%) for sheep and 93.7 % (CI95 %: 87.6-99.7%) for goats. The generalized estimating equation analysis showed that presence of cats and existence of previous reproductive disorders were risk factors potentially associated with T. gondii seropositivity in Small Ruminants. Two statistically significant spatial clusters (P < 0.001) were identified. The seroprevalence observed in the present study indicates a widespread exposure to T. gondii in sheep and goats in southern Spain, which might have important implications for animal and public health. Management measures should be implemented in Small ruminant farms in this region in order to reduce the risk of T. gondii infections, particularly in those areas identified in the spatial analysis.
Ignacio Garciabocanegra - One of the best experts on this subject based on the ideXlab platform.
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epidemiological surveillance of schmallenberg virus in Small Ruminants in southern spain
Transboundary and Emerging Diseases, 2020Co-Authors: Debora Jimenezmartin, David Canoterriza, Jose M Diazcao, Joan Pujols, Manuel Fernandezmorente, Ignacio GarciabocanegraAbstract:Schmallenberg virus (SBV) is an emerging Culicoides-borne Orthobunyavirus that affects ruminant species. Between 2011 and 2013, it was responsible for a large-scale epidemic in Europe. In the present study, we aimed to determine the seroprevalence, spatial distribution and risk factors associated with SBV exposure in sheep and goats in the region where the first Schmallenberg disease outbreak in Spain was reported. Blood samples from 1,796 Small Ruminants from 120 farms were collected in Andalusia (southern Spain) between 2015 and 2017. Antibodies against SBV were detected in 536 of 1,796 animals (29.8%; 95%CI: 27.7-32.0) using a commercial blocking ELISA. The individual seroprevalence according to species was 31.1% (280/900; 95%CI: 28.1-34.1) in sheep and 28.6% (256/896; 95%CI: 25.6-31.5) in goats. The farm prevalence was 76.7% (95%CI: 69.1-84.2). Seropositivity to SBV was confirmed in both sheep and goats in all provinces by virus neutralization test. Two significant (p 14oC) and altitude (<400 metres above sea level) were risk factors associated with SBV exposure in Small Ruminants. Our results highlight widespread but not homogeneous circulation of SBV in Small ruminant populations in Spain.
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epidemiological surveillance of toxoplasma gondii in Small Ruminants in southern spain
Preventive Veterinary Medicine, 2020Co-Authors: Debora Jimenezmartin, Ignacio Garciabocanegra, S Almeria, Sabrina Castroscholten, J P Dubey, M A Amarolopez, David CanoterrizaAbstract:Toxoplasmosis, caused by the protozoan Toxoplasma gondii, is a worldwide zoonotic disease, which affects most warm-blooded species. Besides the zoonotic relevance, toxoplasmosis is one of the major causes of reproductive disorders in Small Ruminants. A cross-sectional study was carried out to determine the seroprevalence, spatial distribution and risk factors associated with T. gondii infection in sheep and goats in southern Spain. During 2015-2017, a total of 1,943 Small Ruminants (998 sheep and 945 goats) from 127 flocks were tested for antibodies against T. gondii using the modified agglutination test (MAT, cut-off 1:25). Antibodies against T. gondii were detected in 464 of the 998 sheep (46.5 %; CI95 %: 43.4-49.6%) and 362 of the 945 goats (38.3 %; CI95 %: 35.2-41.4%) tested. The farm prevalence was 98.4 % (CI95 %: 95.4-100%) for sheep and 93.7 % (CI95 %: 87.6-99.7%) for goats. The generalized estimating equation analysis showed that presence of cats and existence of previous reproductive disorders were risk factors potentially associated with T. gondii seropositivity in Small Ruminants. Two statistically significant spatial clusters (P < 0.001) were identified. The seroprevalence observed in the present study indicates a widespread exposure to T. gondii in sheep and goats in southern Spain, which might have important implications for animal and public health. Management measures should be implemented in Small ruminant farms in this region in order to reduce the risk of T. gondii infections, particularly in those areas identified in the spatial analysis.
Debora Jimenezmartin - One of the best experts on this subject based on the ideXlab platform.
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epidemiological surveillance of schmallenberg virus in Small Ruminants in southern spain
Transboundary and Emerging Diseases, 2020Co-Authors: Debora Jimenezmartin, David Canoterriza, Jose M Diazcao, Joan Pujols, Manuel Fernandezmorente, Ignacio GarciabocanegraAbstract:Schmallenberg virus (SBV) is an emerging Culicoides-borne Orthobunyavirus that affects ruminant species. Between 2011 and 2013, it was responsible for a large-scale epidemic in Europe. In the present study, we aimed to determine the seroprevalence, spatial distribution and risk factors associated with SBV exposure in sheep and goats in the region where the first Schmallenberg disease outbreak in Spain was reported. Blood samples from 1,796 Small Ruminants from 120 farms were collected in Andalusia (southern Spain) between 2015 and 2017. Antibodies against SBV were detected in 536 of 1,796 animals (29.8%; 95%CI: 27.7-32.0) using a commercial blocking ELISA. The individual seroprevalence according to species was 31.1% (280/900; 95%CI: 28.1-34.1) in sheep and 28.6% (256/896; 95%CI: 25.6-31.5) in goats. The farm prevalence was 76.7% (95%CI: 69.1-84.2). Seropositivity to SBV was confirmed in both sheep and goats in all provinces by virus neutralization test. Two significant (p 14oC) and altitude (<400 metres above sea level) were risk factors associated with SBV exposure in Small Ruminants. Our results highlight widespread but not homogeneous circulation of SBV in Small ruminant populations in Spain.
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epidemiological surveillance of toxoplasma gondii in Small Ruminants in southern spain
Preventive Veterinary Medicine, 2020Co-Authors: Debora Jimenezmartin, Ignacio Garciabocanegra, S Almeria, Sabrina Castroscholten, J P Dubey, M A Amarolopez, David CanoterrizaAbstract:Toxoplasmosis, caused by the protozoan Toxoplasma gondii, is a worldwide zoonotic disease, which affects most warm-blooded species. Besides the zoonotic relevance, toxoplasmosis is one of the major causes of reproductive disorders in Small Ruminants. A cross-sectional study was carried out to determine the seroprevalence, spatial distribution and risk factors associated with T. gondii infection in sheep and goats in southern Spain. During 2015-2017, a total of 1,943 Small Ruminants (998 sheep and 945 goats) from 127 flocks were tested for antibodies against T. gondii using the modified agglutination test (MAT, cut-off 1:25). Antibodies against T. gondii were detected in 464 of the 998 sheep (46.5 %; CI95 %: 43.4-49.6%) and 362 of the 945 goats (38.3 %; CI95 %: 35.2-41.4%) tested. The farm prevalence was 98.4 % (CI95 %: 95.4-100%) for sheep and 93.7 % (CI95 %: 87.6-99.7%) for goats. The generalized estimating equation analysis showed that presence of cats and existence of previous reproductive disorders were risk factors potentially associated with T. gondii seropositivity in Small Ruminants. Two statistically significant spatial clusters (P < 0.001) were identified. The seroprevalence observed in the present study indicates a widespread exposure to T. gondii in sheep and goats in southern Spain, which might have important implications for animal and public health. Management measures should be implemented in Small ruminant farms in this region in order to reduce the risk of T. gondii infections, particularly in those areas identified in the spatial analysis.
Munir Aktas - One of the best experts on this subject based on the ideXlab platform.
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Molecular identification, genetic diversity and distribution of Theileria and Babesia species infecting Small Ruminants.
Veterinary parasitology, 2007Co-Authors: Kursat Altay, Nazir Dumanli, Munir AktasAbstract:Detection and identification of Theileria and Babesia species in 920 apparently healthy Small Ruminants in eastern Turkey, as well as parasite genetic diversity, was investigated using a specifically designed reverse line blot (RLB) assay. The hypervariable V4 region of the 18S ribosomal RNA (rRNA) gene was amplified and hybridized to a membrane onto which catchall and species-specific oligonucleotide probes were covalently linked. Three Theileria and one Babesia genotype were identified. Comparison of the Theileria genotypes revealed 93.6-96.2% similarity among their 18S rRNA genes. Two Theileria shared 100% and 99.7% similarity with the previously described sequences of T. ovis and Theileria sp. OT3, respectively. A third Theileria genotype was found to be clearly different from previously described Theileria species. The genotype was provisionally designated as Theileria sp. MK. The Babesia genotype shared 100% similarity with Babesia ovis. The survey indicated a high prevalence of piroplasm infections in Small Ruminants (38.36%). Theileria spp. prevalence was 36.08%. Prevalence of B. ovis was 5.43%. The most abundant Theileria species identified was T. ovis (34.56%) followed by Theileia sp. MK (1.30%) and Theileria sp. OT3 (0.43%).
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determination of prevalence and risk factors for infection with babesia ovis in Small Ruminants from turkey by polymerase chain reaction
Parasitology Research, 2007Co-Authors: Munir Aktas, Kursat Altay, Nazir DumanliAbstract:In this study, PCR and thin blood smear-based diagnostic methods were used to assess the frequency of Babesia infection in Small Ruminants. A total of 300 sheep and 100 goats from 37 randomly selected herds located in eight locations of eastern Turkey were examined for the presence of Babesia infection and any tick species on the body of the animals. Of 400 blood samples examined, 6 (1.5%) were positive for Babesia spp. piroplasms upon microscopic examination, whereas 33 (8.25%) were positive for the presence of B. ovis by PCR. The prevalence of babesiosis in Small Ruminants detected by PCR was significantly higher than obtained in microscopic examination of thin blood smears (P 0.05). The frequency of B. ovis infection was higher in herds with tick burden than no tick burden (P < 0.05). Seven amplicons (six from sheep and one from goat) were sequenced. The resulting sequences were identical to the recently reported nucleotide sequence of B. ovis. A total of 510 ticks belonging to the Rhipicephalus spp. were collected from sheep. Ticks were identified to be R. bursa and R. turanicus on the basis of morphological features.
S Almeria - One of the best experts on this subject based on the ideXlab platform.
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epidemiological surveillance of toxoplasma gondii in Small Ruminants in southern spain
Preventive Veterinary Medicine, 2020Co-Authors: Debora Jimenezmartin, Ignacio Garciabocanegra, S Almeria, Sabrina Castroscholten, J P Dubey, M A Amarolopez, David CanoterrizaAbstract:Toxoplasmosis, caused by the protozoan Toxoplasma gondii, is a worldwide zoonotic disease, which affects most warm-blooded species. Besides the zoonotic relevance, toxoplasmosis is one of the major causes of reproductive disorders in Small Ruminants. A cross-sectional study was carried out to determine the seroprevalence, spatial distribution and risk factors associated with T. gondii infection in sheep and goats in southern Spain. During 2015-2017, a total of 1,943 Small Ruminants (998 sheep and 945 goats) from 127 flocks were tested for antibodies against T. gondii using the modified agglutination test (MAT, cut-off 1:25). Antibodies against T. gondii were detected in 464 of the 998 sheep (46.5 %; CI95 %: 43.4-49.6%) and 362 of the 945 goats (38.3 %; CI95 %: 35.2-41.4%) tested. The farm prevalence was 98.4 % (CI95 %: 95.4-100%) for sheep and 93.7 % (CI95 %: 87.6-99.7%) for goats. The generalized estimating equation analysis showed that presence of cats and existence of previous reproductive disorders were risk factors potentially associated with T. gondii seropositivity in Small Ruminants. Two statistically significant spatial clusters (P < 0.001) were identified. The seroprevalence observed in the present study indicates a widespread exposure to T. gondii in sheep and goats in southern Spain, which might have important implications for animal and public health. Management measures should be implemented in Small ruminant farms in this region in order to reduce the risk of T. gondii infections, particularly in those areas identified in the spatial analysis.
