The Experts below are selected from a list of 24 Experts worldwide ranked by ideXlab platform
Aparna Gomes - One of the best experts on this subject based on the ideXlab platform.
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viper and cobra Venom neutralization by β sitosterol and stigmasterol isolated from the root extract of pluchea indica less asteraceae
Phytomedicine, 2007Co-Authors: Aparna Gomes, Ipshita Chatterjee, Archita Saha, A K ChakravartyAbstract:Abstract We reported previously that the methanolic root extract of the Indian medicinal plant Pluchea indica Less. (Asteraceae) could neutralize viper Venom-induced action [Alam, M.I., Auddy, B., Gomes, A., 1996. Viper Venom neutralization by Indian medicinal plant (Hemidesmus indicus and P. indica) root extracts. Phytother. Res. 10, 58–61]. The present study reports the neutralization of viper and cobra Venom by β-sitosterol and stigmasterol isolated from the root extract of P. indica Less. (Asteraceae). The active fraction (containing the major compound β-sitosterol and the minor compound stigmasterol) was isolated and purified by silica gel column chromatography and the structure was determined using spectroscopic analysis (EIMS, 1H NMR, 13C NMR). Anti-Snake Venom activity was studied in experimental animals. The active fraction was found to significantly neutralize viper Venom-induced lethal, hemorrhagic, defibrinogenation, edema and PLA2 activity. Cobra Venom-induced lethality, cardiotoxicity, neurotoxicity, respiratory changes and PLA2 activity were also antagonized by the active component. It potentiated commercial Snake Venom Antiserum action against Venom-induced lethality in male albino mice. The active fraction could antagonize Venom-induced changes in lipid peroxidation and superoxide dismutase activity. This study suggests that β-sitosterol and stigmasterol may play an important role, along with Antiserum, in neutralizing Snake Venom-induced actions.
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adjuvant effects and Antiserum action potentiation by a herbal compound 2 hydroxy 4 methoxy benzoic acid isolated from the root extract of the indian medicinal plant sarsaparilla hemidesmus indicus r br
Toxicon, 1998Co-Authors: M.i. Alam, Aparna GomesAbstract:The adjuvant effect and Antiserum potentiation of a compound 2-hydroxy-4-methoxy benzoic acid were explored in the present investigation. This compound, isolated and purified from the Indian medicinal plant Hemidesmus indicus R. Br, possessed antiSnake Venom activity. Rabbits immunized with Vipera russellii Venom in the presence and absence of the compound along with Freund's complete adjuvant, produced a precipitating band in immunogel diffusion and immunogel electrophoresis. The Venom neutralizing capacity of this Antiserum showed positive adjuvant effects as evident by the higher neutralization capacity (lethal and hemorrhage) when compared with the Antiserum raised with Venom alone. The pure compound potentiated the lethal action neutralization of Venom by commercial equine polyvalent Snake Venom Antiserum in experimental models. These observations raised the possibility of the use of chemical antagonists (from herbs) against Snake bite, which may provide a better protection in presence of Antiserum, especially in the rural parts of India.
A K Chakravarty - One of the best experts on this subject based on the ideXlab platform.
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viper and cobra Venom neutralization by β sitosterol and stigmasterol isolated from the root extract of pluchea indica less asteraceae
Phytomedicine, 2007Co-Authors: Aparna Gomes, Ipshita Chatterjee, Archita Saha, A K ChakravartyAbstract:Abstract We reported previously that the methanolic root extract of the Indian medicinal plant Pluchea indica Less. (Asteraceae) could neutralize viper Venom-induced action [Alam, M.I., Auddy, B., Gomes, A., 1996. Viper Venom neutralization by Indian medicinal plant (Hemidesmus indicus and P. indica) root extracts. Phytother. Res. 10, 58–61]. The present study reports the neutralization of viper and cobra Venom by β-sitosterol and stigmasterol isolated from the root extract of P. indica Less. (Asteraceae). The active fraction (containing the major compound β-sitosterol and the minor compound stigmasterol) was isolated and purified by silica gel column chromatography and the structure was determined using spectroscopic analysis (EIMS, 1H NMR, 13C NMR). Anti-Snake Venom activity was studied in experimental animals. The active fraction was found to significantly neutralize viper Venom-induced lethal, hemorrhagic, defibrinogenation, edema and PLA2 activity. Cobra Venom-induced lethality, cardiotoxicity, neurotoxicity, respiratory changes and PLA2 activity were also antagonized by the active component. It potentiated commercial Snake Venom Antiserum action against Venom-induced lethality in male albino mice. The active fraction could antagonize Venom-induced changes in lipid peroxidation and superoxide dismutase activity. This study suggests that β-sitosterol and stigmasterol may play an important role, along with Antiserum, in neutralizing Snake Venom-induced actions.
Ipshita Chatterjee - One of the best experts on this subject based on the ideXlab platform.
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viper and cobra Venom neutralization by β sitosterol and stigmasterol isolated from the root extract of pluchea indica less asteraceae
Phytomedicine, 2007Co-Authors: Aparna Gomes, Ipshita Chatterjee, Archita Saha, A K ChakravartyAbstract:Abstract We reported previously that the methanolic root extract of the Indian medicinal plant Pluchea indica Less. (Asteraceae) could neutralize viper Venom-induced action [Alam, M.I., Auddy, B., Gomes, A., 1996. Viper Venom neutralization by Indian medicinal plant (Hemidesmus indicus and P. indica) root extracts. Phytother. Res. 10, 58–61]. The present study reports the neutralization of viper and cobra Venom by β-sitosterol and stigmasterol isolated from the root extract of P. indica Less. (Asteraceae). The active fraction (containing the major compound β-sitosterol and the minor compound stigmasterol) was isolated and purified by silica gel column chromatography and the structure was determined using spectroscopic analysis (EIMS, 1H NMR, 13C NMR). Anti-Snake Venom activity was studied in experimental animals. The active fraction was found to significantly neutralize viper Venom-induced lethal, hemorrhagic, defibrinogenation, edema and PLA2 activity. Cobra Venom-induced lethality, cardiotoxicity, neurotoxicity, respiratory changes and PLA2 activity were also antagonized by the active component. It potentiated commercial Snake Venom Antiserum action against Venom-induced lethality in male albino mice. The active fraction could antagonize Venom-induced changes in lipid peroxidation and superoxide dismutase activity. This study suggests that β-sitosterol and stigmasterol may play an important role, along with Antiserum, in neutralizing Snake Venom-induced actions.
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antiSnake Venom activity of ethanolic seed extract of strychnos nux vomica linn
Indian Journal of Experimental Biology, 2004Co-Authors: Ipshita Chatterjee, Amiya K. ChakravartyAbstract:The whole seed extract of S.nux vomica (in low doses) effectively neutralized Daboia russelii Venom induced lethal, haemorrhage, defibrinogenating, PLAN enzyme activity and Naja kaouthia Venom induced lethal, cardiotoxic,neurotoxic, PLA 2 enzyme activity. The seed extract potentiated polyvalent Snake Venom Antiserum action in experimental animals. An active compound (SNVNF) was isolated and purified by thin layer chromatography and silica gel column chromatography, which effectively antagonised D. russelii Venom induced lethal, haemorrhagic, defibrinogenating, oedema, PLAN enzyme activity and N. kaouthia induced lethal, cardiotoxic, neurotoxic, PLA 2 enzyme activity. Polyvalent Snake Venom Antiserum action was significantly potentiated by the active compound. Spectral studies revealed it to be a small, straight chain compound containing methyl and amide radicals. Detailed structure elucidation of the compound (SNVNF) is warranted before its clinical trials as a Snake Venom antagonist.
Archita Saha - One of the best experts on this subject based on the ideXlab platform.
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viper and cobra Venom neutralization by β sitosterol and stigmasterol isolated from the root extract of pluchea indica less asteraceae
Phytomedicine, 2007Co-Authors: Aparna Gomes, Ipshita Chatterjee, Archita Saha, A K ChakravartyAbstract:Abstract We reported previously that the methanolic root extract of the Indian medicinal plant Pluchea indica Less. (Asteraceae) could neutralize viper Venom-induced action [Alam, M.I., Auddy, B., Gomes, A., 1996. Viper Venom neutralization by Indian medicinal plant (Hemidesmus indicus and P. indica) root extracts. Phytother. Res. 10, 58–61]. The present study reports the neutralization of viper and cobra Venom by β-sitosterol and stigmasterol isolated from the root extract of P. indica Less. (Asteraceae). The active fraction (containing the major compound β-sitosterol and the minor compound stigmasterol) was isolated and purified by silica gel column chromatography and the structure was determined using spectroscopic analysis (EIMS, 1H NMR, 13C NMR). Anti-Snake Venom activity was studied in experimental animals. The active fraction was found to significantly neutralize viper Venom-induced lethal, hemorrhagic, defibrinogenation, edema and PLA2 activity. Cobra Venom-induced lethality, cardiotoxicity, neurotoxicity, respiratory changes and PLA2 activity were also antagonized by the active component. It potentiated commercial Snake Venom Antiserum action against Venom-induced lethality in male albino mice. The active fraction could antagonize Venom-induced changes in lipid peroxidation and superoxide dismutase activity. This study suggests that β-sitosterol and stigmasterol may play an important role, along with Antiserum, in neutralizing Snake Venom-induced actions.
M.i. Alam - One of the best experts on this subject based on the ideXlab platform.
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adjuvant effects and Antiserum action potentiation by a herbal compound 2 hydroxy 4 methoxy benzoic acid isolated from the root extract of the indian medicinal plant sarsaparilla hemidesmus indicus r br
Toxicon, 1998Co-Authors: M.i. Alam, Aparna GomesAbstract:The adjuvant effect and Antiserum potentiation of a compound 2-hydroxy-4-methoxy benzoic acid were explored in the present investigation. This compound, isolated and purified from the Indian medicinal plant Hemidesmus indicus R. Br, possessed antiSnake Venom activity. Rabbits immunized with Vipera russellii Venom in the presence and absence of the compound along with Freund's complete adjuvant, produced a precipitating band in immunogel diffusion and immunogel electrophoresis. The Venom neutralizing capacity of this Antiserum showed positive adjuvant effects as evident by the higher neutralization capacity (lethal and hemorrhage) when compared with the Antiserum raised with Venom alone. The pure compound potentiated the lethal action neutralization of Venom by commercial equine polyvalent Snake Venom Antiserum in experimental models. These observations raised the possibility of the use of chemical antagonists (from herbs) against Snake bite, which may provide a better protection in presence of Antiserum, especially in the rural parts of India.