The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform

K Syamasundar - One of the best experts on this subject based on the ideXlab platform.

Adharvana M Chari - One of the best experts on this subject based on the ideXlab platform.

Janis Yaxian Zhan - One of the best experts on this subject based on the ideXlab platform.

  • andrographolide Sodium Bisulfate attenuates uv induced photo damage by activating the keap1 nrf2 pathway and downregulating the nf κb pathway in hacat keratinocytes
    International Journal of Molecular Medicine, 2019
    Co-Authors: Mei Ling Wang, Qing Yuan Zhong, Bao Qin Lin, Yuhong Liu, Yan Feng Huang, Yang Chen, Jie Yuan, Janis Yaxian Zhan
    Abstract:

    Oxidative and inflammatory damage has been suggested to play important roles in the pathogenesis of skin photoaging. Andrographolide Sodium Bisulfate (ASB) is a soluble derivative of andrographolide and has known antioxidant and anti‑inflammatory properties. In the present study, cellular experiments were designed to investigate the molecular mechanisms underlying the effect of ASB in relieving ultraviolet (UV)‑induced photo‑damage. Following ASB pretreatment and UV irradiation, the apoptosis and necrosis of HaCaT cells were investigated by Hoechst 33342/propidium iodide staining. Reactive oxygen species (ROS) production was investigated using a DCFH‑DA fluorescence probe. Furthermore, the protein expression levels of p65, NF‑κB inhibitor‑α, nuclear factor E2‑related factor 2 (Nrf2) and kelch‑like ECH‑associated protein 1 (keap1) were measured via western blotting and immunofluorescence analyses. Furthermore, NF‑κB‑mediated cytokines were assessed by ELISA, and Nrf2‑mediated genes were detected by reverse transcription‑quantitative PCR. Pretreatment with ASB markedly increased cell viability, decreased cell apoptosis and decreased UV‑induced excess ROS levels. In addition, ASB activated the production of Nrf2 and increased the mRNA expression levels of glutamate‑cysteine ligase catalytic subunit and NAD(P)H quinone oxidoreductase 1, while ASB downregulated the protein expression of p65 and decreased the production of interleukin (IL)‑1β, IL‑6 and tumor necrosis factor‑α. These results suggested that ASB attenuates UV‑induced photo‑damage by activating the keap1/Nrf2 pathway and downregulating the NF‑κB pathway in HaCaT keratinocytes.

  • andrographolide Sodium Bisulfate prevents uv induced skin photoaging through inhibiting oxidative stress and inflammation
    Mediators of Inflammation, 2016
    Co-Authors: Janis Yaxian Zhan, Xiufen Wang, Zhenbiao Zhang, Lan Wang, Jiannan Chen, Song Huang, Huifang Zeng
    Abstract:

    Andrographolide Sodium Bisulfate (ASB), a water-soluble form made from andrographolide through sulfonating reaction, is an antioxidant and anti-inflammatory drug; however, the antiphotoaging effect of ASB has still not been revealed. Oxidative stress and inflammation are known to be responsible for ultraviolet (UV) irradiation induced skin damage and consequently premature aging. In this study, we aimed at examining the effect of ASB on UV-induced skin photoaging of mice by physiological and histological analysis of skin and examination of skin antioxidant enzymes and immunity analyses. Results showed that topical administration of ASB suppressed the UV-induced skin thickness, elasticity, wrinkles, and water content, while ASB, especially at dose of 3.6 mg/mouse, increased the skin collagen content by about 53.17%, decreased the epidermal thickness by about 41.38%, and prevented the UV-induced disruption of collagen fibers and elastic fibers. Furthermore, ASB decreased MDA level by about 40.21% and upregulated the activities of SOD and CAT and downregulated the production of IL-1β, IL-6, IL-10, and TNF-α in UV-irradiated mice. Our study confirmed the protective effect of ASB against UV-induced photoaging and initially indicated that this effect can be attributed to its antioxidant and anti-inflammatory activities in vivo, suggesting that ASB may be a potential antiphotoaging agent.

Frank M Mitloehner - One of the best experts on this subject based on the ideXlab platform.

  • effects of Sodium Bisulfate on the bacterial population structure of dairy cow waste
    Journal of Applied Microbiology, 2011
    Co-Authors: Jeffery A Mcgarvey, Kimberly R Stackhouse, William G Miller, Larry H Stanker, Robert Hnasko, Frank M Mitloehner
    Abstract:

    Aims:  To determine the effects of Sodium Bisulfate (SBS) on the bacterial populations in cattle waste. Methods and Results:  We applied SBS at 0, 60, 70 or 100 kg week−1 to cattle waste as it accumulated on the floors of four cattle pens, housing eight cattle each. We observed significant pH decreases in all of the treated wastes on day one; however, the 60 kg week−1 treatment returned to control levels by day four, while the others remained significantly lower. Heterotrophic plate counts of the waste revealed that all treatments reduced the bacterial populations in the wastes on day one; however, all returned to control levels by day four. The 16S rRNA gene libraries derived from the wastes revealed significant reductions in sequences associated with the phyla Bacteroidetes and Firmicutes and increases in the Proteobacteria, Actinobacteria and Spirochaetes on day one, but resembled the control by day seven. Sequences associated with Escherichia coli increased significantly after SBS application, but became undetectable by day seven. Conclusions:  SBS application significantly alters the bacterial population structure of waste during the first few days of application, but the populations return to almost normal after 7 days. Significance and Impact of the Study:  Application of SBS to animal waste can reduce emissions; however, biosecurity precautions must be rigorously maintained during the initial application to ensure that pathogenic E. coli is not released into the environment.

  • effects of Sodium Bisulfate on alcohol amine and ammonia emissions from dairy slurry
    Journal of Environmental Quality, 2008
    Co-Authors: Huawei Sun, Yuee Pan, Yongjing Zhao, Wendi Jackson, Lisa M Nuckles, Irina L Malkina, Veronica Arteaga, Frank M Mitloehner
    Abstract:

    Sodium Bisulfate (SBS) is extensively used in the poultry industry to reduce ammonia and bacterial levels in litter. It is also used in the dairy industry to reduce bacterial counts in bedding and ammonia emissions, preventing environmental mastitis and calf respiratory stress. The present study measured the effect of SBS on the air emission of ammonia, amine, and alcohol from a dairy slurry mix. Amine flux was undetectable (<5 ng L(-1)) across treatments. Application of SBS decreased ammonia, methanol, and ethanol emissions from fresh dairy slurry. Ammonia emissions decreased with increasing levels of SBS treatment. The 3-d average ammonia flux from the control (no SBS applied) and the three different SBS surface application levels of 0.125, 0.250, and 0.375 kg m(-2) were 513.4, 407.2, 294.8, and 204.5 mg h(-1) m(-2), respectively. The ammonia emission reduction potentials were 0, 21, 43, and 60%, respectively. Methanol and ethanol emissions decreased with an increase in the amount of SBS applied. The 3-d average methanol emissions were 223.7, 178.0, 131.6, and 87.0 mg h(-1) m(-2) for SBS surface application level of 0, 0.125, 0.250, and 0.375 kg m(-2), with corresponding reduction potentials of 0, 20, 41, and 61, respectively. Similar emission reduction potentials of 0, 18, 35, and 58% were obtained for ethanol. Sodium Bisulfate was shown to be effective in the mitigation of ammonia and alcohol emissions from fresh dairy slurry.

Janak Dhakal - One of the best experts on this subject based on the ideXlab platform.

  • Significance of Sodium Bisulfate (SBS) Tempering in Reducing the Escherichia coli O121 and O26 Load of Wheat and Its Effects on Wheat Flour Quality
    MDPI AG, 2021
    Co-Authors: Jared Rivera, Aiswariya Deliephan, Janak Dhakal, Charles Gregory Aldrich, Kaliramesh Siliveru
    Abstract:

    The occurrence of recalls involving pathogenic Escherichia coli-contaminated wheat flours show the need for incorporating antimicrobial interventions in wheat milling. The objectives of this study were to assess the efficacy of Sodium Bisulfate (SBS) tempering in reducing E. coli O121 (ATCC 2219) and O26 (ATCC 2196) wheat load and to evaluate the impact of effective (≥3.0 log reductions) SBS treatments on wheat flour quality. Wheat grains were inoculated with E. coli (~6 log CFU/g) and tempered (17% moisture, 24 h) using the following SBS concentrations (%wheat basis): 0, 0.5, 0.75, 1.0, 1.25, and 1.5% SBS. Reductions in E. coli O121 and O26 wheat load at different time intervals (0.5, 2, 6, 12, 18, and 24 h) during tempering were evaluated. The addition of SBS during tempering resulted in E. coli (O121 and O26) log reductions of 2.0 (0.5% SBS) to >4.0 logs (1.5% SBS) (p ≤ 0.05). SBS tempering (1.25 and 1.5% SBS) produced acidic wheat flours (pH = 4.51–4.60) but had comparable wheat flour properties in terms of composition, dough, and bread-making properties relative to the control (0% SBS). SBS tempering reduced the E. coli O121 and O26 load of wheat after tempering with minimal effects on wheat flour quality.

  • A Comparison of Salmonella Survival and Detection Using an Enrichment Technique in Dry- and Wet-Inoculated Rendered Chicken Fat Treated with Sodium Bisulfate
    International Association for Food Protection, 2021
    Co-Authors: Janak Dhakal, Charles G Aldrich
    Abstract:

    ABSTRACT The differences in the recovery of Salmonella from rendered chicken fat treated with Sodium Bisulfate (SBS) when inoculated with a dry versus wet inoculum were evaluated. Food-grade rendered chicken fat was inoculated with a dry inoculum and a wet inoculum containing a cocktail of Salmonella serovars (Enteritidis, Heidelberg, and Typhimurium). In addition, the effect of an antimicrobial treatment (SBS) against Salmonella in both the aqueous phase and fat phase of the chicken fat was evaluated. The untreated control samples in the aqueous phase had a consistent level of Salmonella (∼7 log) when both the dry and wet inocula were used. In the SBS-treated aqueous phase, Salmonella pathogens were not detectable after 6 h when the wet inoculum was used; when the dry inoculum was used, Salmonella pathogens were not detectable at 24 h. Salmonella pathogens were detected for up to 6 h in the SBS-treated fat phase when the dry inoculum was used compared with 2 h with the wet inoculum. The 24-h fat samples that failed to show growth on Trypticase soy agar were enriched for Salmonella isolation, followed by confirmation by PCR using primers for the invA gene. SBS-treated and control samples from the dry-inoculated rendered chicken fat and the inoculated control from the wet-inoculated rendered chicken fat tested positive for Salmonella. However, the SBS-treated sample from the wet-inoculated fat was negative for Salmonella. The use of dry SBS powder against dry Salmonella inoculum in the fat matrix caused only ∼2.8-log reduction after 24 h compared with ∼2.2-log reduction in the positive control. However, the recovery of Salmonella from untreated control fat was lower and was not different (P &gt; 0.05) from that recovered from the SBS-treated fat. The results suggest that viable but nonculturable states of Salmonella may develop in rendered chicken fat or that injured cells may be present, which indicates that testing should include an enrichment and appropriate molecular confirmation instead of agar plating alone. HIGHLIGHTS

  • assessing the efficacy of Sodium Bisulfate and organic acid treatments for control of salmonella typhimurium in rendered chicken fat applied to pet foods
    Journal of Food Protection, 2019
    Co-Authors: Janak Dhakal, Charles G Aldrich, Carl Knueven
    Abstract:

    This study was conducted to evaluate the effects of Sodium Bisulfate (SBS), lactic acid (LA), phosphoric acid (PA), and combinations of organic acids with SBS on Salmonella in rendered chicken fat and in water. The MICs of the antimicrobials individually and in combination were determined. Efficacies of the antimicrobials against Salmonella were tested in both media. The MICs of SBS, LA, and PA were 0.5, 0.5, and 0.25%, respectively. At the given concentrations in the water phase, 0.5% SBS was more effective (P 4-log reductions at 2 h and complete kill at 6 h. After 24 h, each of the chemicals completely eliminated the Salmonella. However, because of low recovery in the fat phase, Salmonella was not detected after 12 h and all three chemicals effectively reduced (P 5.5 log CFU/mL after 12 h. The SBS+LA combinations were effective (P < 0.05) after 2 h. The combinations of SBS+PA resulted in ∼3.5-log reductions in Salmonella (P < 0.05) after 6 h. In the fat phase, except for the SBS+PA combination, Salmonella reduction was not different from that for the positive control. When SBS was combined with organic acids, Salmonella inhibition was achieved at a lower SBS concentration, indicating a possibly synergistic effect of these chemicals. These results suggest that inclusion of SBS or LA at 0.5% individually or a combination of SBS with organic acids could reduce Salmonella in rendered chicken fat contaminated by residual water encountered during storage and transport.