The Experts below are selected from a list of 1272 Experts worldwide ranked by ideXlab platform
Deborah Pacetti - One of the best experts on this subject based on the ideXlab platform.
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a quantitative headspace solid phase microextraction gas chromatography flame ionization detector method to analyze short chain free fatty acids in rat feces
Analytical Biochemistry, 2016Co-Authors: Dennis Fiorini, Maria Chiara Boarelli, Rosita Gabbianelli, Roberto Ballini, Deborah PacettiAbstract:Abstract This study sought to develop and validate a quantitative method to analyze short chain free fatty acids (SCFAs) in rat feces by solid-phase microextraction and gas chromatography (SPME–GC) using the salt mixture ammonium sulfate and Sodium Dihydrogen Phosphate as salting out agent. Conditioning and extraction time, linearity, limits of detection and quantification, repeatability, and recovery were evaluated. The proposed method allows quantification with improved sensitivity as compared with other methods exploiting SPME–GC. The method has been applied to analyze rat fecal samples, quantifying acetic, propionic, isobutyric, butyric, isopentanoic, pentanoic, and hexanoic acids.
Dennis Fiorini - One of the best experts on this subject based on the ideXlab platform.
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a quantitative headspace solid phase microextraction gas chromatography flame ionization detector method to analyze short chain free fatty acids in rat feces
Analytical Biochemistry, 2016Co-Authors: Dennis Fiorini, Maria Chiara Boarelli, Rosita Gabbianelli, Roberto Ballini, Deborah PacettiAbstract:Abstract This study sought to develop and validate a quantitative method to analyze short chain free fatty acids (SCFAs) in rat feces by solid-phase microextraction and gas chromatography (SPME–GC) using the salt mixture ammonium sulfate and Sodium Dihydrogen Phosphate as salting out agent. Conditioning and extraction time, linearity, limits of detection and quantification, repeatability, and recovery were evaluated. The proposed method allows quantification with improved sensitivity as compared with other methods exploiting SPME–GC. The method has been applied to analyze rat fecal samples, quantifying acetic, propionic, isobutyric, butyric, isopentanoic, pentanoic, and hexanoic acids.
Weisheng Guan - One of the best experts on this subject based on the ideXlab platform.
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liquid liquid equilibria of the aqueous two phase systems composed of the n ethylpyridinium tetrafluoroborate ionic liquid and ammonium sulfate anhydrous Sodium carbonate Sodium Dihydrogen Phosphate and water at 298 15 k
Journal of Chemical & Engineering Data, 2014Co-Authors: Xiao Shu, Xuewen Zhang, Weisheng GuanAbstract:In this work, the phase diagrams and the liquid–liquid equilibrium (LLE) data at 298.15 K of the aqueous [EPy]BF4 + ammonium sulfate + H2O, [EPy]BF4 + Sodium Dihydrogen Phosphate + H2O, and [EPy]BF4 + Sodium carbonate + H2O two-phase systems were experimentally determined. The Merchuk equation was used to correlate the binodal data of the investigated systems. The tie-line data were correlated according to the Othmer–Tobias and Bancroft equations, the Setschenow equation, and a two-parameter equation. The results indicate that all of these equations can be successfully used to correlate the experimental data of the investigated systems at 298.15 K. Both the calculated effective excluded volume (EEV) and the binodal curves, which were plotted using the mass fractions, indicate the phase-separation abilities of the investigated salts, which follow the following order: Na2CO3 > (NH4)2SO4 > NaH2PO4.
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liquid liquid equilibria of aqueous two phase systems of the ionic liquid brominated n ethyl pyridine and Sodium Dihydrogen Phosphate Sodium sulfate ammonium citrate and potassium tartrate at different temperatures experimental determination and corr
Fluid Phase Equilibria, 2013Co-Authors: Yuliang Li, Hang Su, Mengshi Zhang, Weisheng GuanAbstract:Abstract Liquid–liquid equilibrium data have been determined experimentally for aqueous two-phase systems of brominated N-ethyl pyridine ([EPy]Br) and Sodium Dihydrogen Phosphate (NaH2PO4) at T = 298.15, 308.15, 318.15, and 328.15 K, and data have also been obtained for aqueous two-phase systems composed of [EPy]Br and Sodium sulfate (Na2SO4), ammonium citrate (C6H5O7(NH4)3) or potassium tartrate (C4H4O6K2) at T = 298.15 K. The Merchuk equation was used to model the binodal data. The effects of temperature on the binodal curves and tie-lines were studied, and the two-phase area was found to expand with a decrease in temperature, while the slope of the tie lines slightly decreased with an increase in temperature. Furthermore, the effective excluded volume (EEV) values obtained from the binodal model for these systems were determined, and the salting-out abilities follow the order of Na2SO4 > NaH2PO4 > C4H4O6K2 > C6H5O7(NH4)3. The Othmer–Tobias, Bancroft and several parameter equations were used to determine the correlations of the tie-line data. These models agreed well with the experimental tie-line data.
Ponnuswamy Vijayaraghavan - One of the best experts on this subject based on the ideXlab platform.
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solid substrate bioprocessing of cow dung for the production of carboxymethyl cellulase by bacillus halodurans ind18
Waste Management, 2016Co-Authors: Ponnuswamy Vijayaraghavan, S Prakash G Vincent, G S DhillonAbstract:The production of carboxymethyl cellulase (CMCase) by Bacillus halodurans IND18 under solid substrate fermentation (SSF) using cow dung was optimized through two level full factorial design and second order response surface methodology (RSM). The central composite design (CCD) was employed to optimize the vital fermentation parameters, such as pH of the substrate, concentration of nitrogen source (peptone) and ion (Sodium Dihydrogen Phosphate) sources in medium for achieving higher enzyme production. The optimum medium composition was found to be 1.46% (w/w) peptone, 0.095% (w/w) Sodium Dihydrogen Phosphate and pH 8.0. The model prediction of 4210IU/g enzyme activity at optimum conditions was verified experimentally as 4140IU/g. The enzyme was active over a broad temperature range (40-60±1°C) and pH (7.0-9.0) with maximal activity at 60±1°C and pH 8.0. This study demonstrated the potential of cow dung as novel substrate for CMCase production.
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Medium Optimization for the Production of Fibrinolytic Enzyme by Paenibacillus sp. IND8 Using Response Surface Methodology
Hindawi Limited, 2014Co-Authors: Ponnuswamy Vijayaraghavan, Samuel Gnana Prakash VincentAbstract:Production of fibrinolytic enzyme by a newly isolated Paenibacillus sp. IND8 was optimized using wheat bran in solid state fermentation. A 25 full factorial design (first-order model) was applied to elucidate the key factors as moisture, pH, sucrose, yeast extract, and Sodium Dihydrogen Phosphate. Statistical analysis of the results has shown that moisture, sucrose, and Sodium Dihydrogen Phosphate have the most significant effects on fibrinolytic enzymes production (P
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Research Article Medium Optimization for the Production of Fibrinolytic Enzyme by Paenibacillus sp. IND8 Using Response Surface Methodology
2014Co-Authors: Ponnuswamy Vijayaraghavan, Samuel Gnana, Prakash VincentAbstract:Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Production of fibrinolytic enzyme by a newly isolated Paenibacillus sp. IND8 was optimized using wheat bran in solid state fermentation. A 25 full factorial design (first-order model) was applied to elucidate the key factors as moisture, pH, sucrose, yeast extract, and Sodium Dihydrogen Phosphate. Statistical analysis of the results has shown that moisture, sucrose, and Sodium Dihydrogen Phosphate have the most significant effects on fibrinolytic enzymes production
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Statistical Optimization of Fibrinolytic Enzyme Production Using Agroresidues by Bacillus cereus IND1 and Its Thrombolytic Activity In Vitro
Hindawi Limited, 2014Co-Authors: Ponnuswamy Vijayaraghavan, Samuel Gnana Prakash VincentAbstract:A potent fibrinolytic enzyme-producing Bacillus cereus IND1 was isolated from the Indian food, rice. Solid-state fermentation was carried out using agroresidues for the production of fibrinolytic enzyme. Among the substrates, wheat bran supported more enzyme production and has been used for the optimized enzyme production by statistical approach. Two-level full-factorial design demonstrated that moisture, supplementation of beef extract, and Sodium Dihydrogen Phosphate have significantly influenced enzyme production (P
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Research Article Statistical Optimization of Fibrinolytic Enzyme Production Using Agroresidues by Bacillus cereus IND1 and Its Thrombolytic Activity In Vitro
2014Co-Authors: Ponnuswamy Vijayaraghavan, Samuel Gnana, Prakash VincentAbstract:Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. A potent fibrinolytic enzyme-producing Bacillus cereus IND1 was isolated from the Indian food, rice. Solid-state fermentation was carried out using agroresidues for the production of fibrinolytic enzyme. Among the substrates, wheat bran supported more enzyme production and has been used for the optimized enzyme production by statistical approach. Two-level full-factorial design demonstrated that moisture, supplementation of beef extract, and Sodium Dihydrogen Phosphate have significantly influenced enzyme production
Patrick Sharrock - One of the best experts on this subject based on the ideXlab platform.
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Production of Sodium Dihydrogen Phosphate Using Sodium Chloride and Orthophosphoric Acid
Industrial and engineering chemistry research, 2015Co-Authors: Doan Pham Minh, Ange Nzihou, Patrick SharrockAbstract:Up-to-date, standard synthesis processes of Sodium Phosphates are based on the neutralization of orthophosphoric acid by Sodium hydroxide or Sodium carbonate. To the best of our knowledge, the reaction between Sodium chloride and orthophosphoric acid has not yet been reported in the literature. This study demonstrated the feasibility of the synthesis of Sodium Dihydrogen Phosphate by the reaction of Sodium chloride with orthophosphoric acid. A detailed parametric study was done, wherein the influence of reaction temperature, air flow rate, water addition flow rate, and reaction time was investigated. As expected, the evacuation of hydrogen chloride (HCl) was crucial for getting high conversion of NaCl to HCl. The highest conversion of NaCl to HCl reached 91% after 24 h of synthesis reaction. Sodium Dihydrogen Phosphate (NaH2PO4) was the main product. PyroPhosphate (P2O74-) was only formed at trace amounts, and any trace of trimetaPhosphate (P3O93-) was observed.
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Sodium Dihydrogen Phosphate Starting From Sodium Chloride and Orthophosphoric Acid Via Cation Resin Exchange
Phosphorus Sulfur and Silicon and the Related Elements, 2015Co-Authors: Doan Pham Minh, Ange Nzihou, Patrick SharrockAbstract:Sodium Phosphates are generally synthesized by neutralization of orthophosphoric acid with Sodium hydroxide. In this paper, we communicate a new synthesis process, wherein Sodium Dihydrogen Phosphate can be obtained from Sodium chloride and orthophosphoric acid, using cation resin exchange at room operational conditions. This opens a new concept for the design of environmentally friendly processes in this inorganic synthesis field.