The Experts below are selected from a list of 159465 Experts worldwide ranked by ideXlab platform
Vladimir Besada - One of the best experts on this subject based on the ideXlab platform.
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Sodium Dodecyl Sulfate free gel electrophoresis/electroelution sorting for peptide fractionation
Journal of Separation Science, 2019Co-Authors: Yassel Ramos, Annia Gonzalez, Yairet Garcia, Aniel Sanchez, Patricia Sosa‐acosta, Yasset Perez-riverol, Lila Castellanos-serra, Jeovanis Gil, Luis Javier González, Vladimir BesadaAbstract:Shotgun proteomics based on peptide fractionation by using liquid chromatography has become the common procedure for proteomic studies, although in the very beginning of the field, protein separation by using electrophoresis was the main tool. Nonetheless, during the last two decades, the electrophoretic techniques for peptide mixtures fractionation have evolved as a result of relevant technological improvements. We also proposed the combination of Sodium Dodecyl Sulfate polyacrylamide gel electrophoresis for protein fractionation and Sodium Dodecyl Sulfate free polyacrylamide gel electrophoresis for peptide separation as a novel procedure for proteomic studies. Here, we present an optimized device for Sodium Dodecyl Sulfate free polyacrylamide gel electrophoresis improving peptide recoveries respect to the established electrophoretic technique off gel electrophoresis meanwhile conserving the excellent resolution described for the former technique in slab gel based systems. The device simultaneously allows the separation and the collection of fractionated peptides in solution.
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Sodium Dodecyl Sulfate free gel electrophoresis electroelution sorting for peptide fractionation
IEEE Journal of Solid-state Circuits, 2019Co-Authors: Yassel Ramos, Annia Gonzalez, Patricia Sosaacosta, Yasset Perezriverol, Yairet Garcia, Lila Castellanosserra, Aniel Sanchez, L. Gonzalez, Vladimir BesadaAbstract:Shotgun proteomics based on peptide fractionation by using liquid chromatography has become the common procedure for proteomic studies, although in the very beginning of the field, protein separation by using electrophoresis was the main tool. Nonetheless, during the last two decades, the electrophoretic techniques for peptide mixtures fractionation have evolved as a result of relevant technological improvements. We also proposed the combination of Sodium Dodecyl Sulfate polyacrylamide gel electrophoresis for protein fractionation and Sodium Dodecyl Sulfate free polyacrylamide gel electrophoresis for peptide separation as a novel procedure for proteomic studies. Here, we present an optimized device for Sodium Dodecyl Sulfate free polyacrylamide gel electrophoresis improving peptide recoveries respect to the established electrophoretic technique off gel electrophoresis meanwhile conserving the excellent resolution described for the former technique in slab gel based systems. The device simultaneously allows the separation and the collection of fractionated peptides in solution.
Yassel Ramos - One of the best experts on this subject based on the ideXlab platform.
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Sodium Dodecyl Sulfate free gel electrophoresis/electroelution sorting for peptide fractionation
Journal of Separation Science, 2019Co-Authors: Yassel Ramos, Annia Gonzalez, Yairet Garcia, Aniel Sanchez, Patricia Sosa‐acosta, Yasset Perez-riverol, Lila Castellanos-serra, Jeovanis Gil, Luis Javier González, Vladimir BesadaAbstract:Shotgun proteomics based on peptide fractionation by using liquid chromatography has become the common procedure for proteomic studies, although in the very beginning of the field, protein separation by using electrophoresis was the main tool. Nonetheless, during the last two decades, the electrophoretic techniques for peptide mixtures fractionation have evolved as a result of relevant technological improvements. We also proposed the combination of Sodium Dodecyl Sulfate polyacrylamide gel electrophoresis for protein fractionation and Sodium Dodecyl Sulfate free polyacrylamide gel electrophoresis for peptide separation as a novel procedure for proteomic studies. Here, we present an optimized device for Sodium Dodecyl Sulfate free polyacrylamide gel electrophoresis improving peptide recoveries respect to the established electrophoretic technique off gel electrophoresis meanwhile conserving the excellent resolution described for the former technique in slab gel based systems. The device simultaneously allows the separation and the collection of fractionated peptides in solution.
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Sodium Dodecyl Sulfate free gel electrophoresis electroelution sorting for peptide fractionation
IEEE Journal of Solid-state Circuits, 2019Co-Authors: Yassel Ramos, Annia Gonzalez, Patricia Sosaacosta, Yasset Perezriverol, Yairet Garcia, Lila Castellanosserra, Aniel Sanchez, L. Gonzalez, Vladimir BesadaAbstract:Shotgun proteomics based on peptide fractionation by using liquid chromatography has become the common procedure for proteomic studies, although in the very beginning of the field, protein separation by using electrophoresis was the main tool. Nonetheless, during the last two decades, the electrophoretic techniques for peptide mixtures fractionation have evolved as a result of relevant technological improvements. We also proposed the combination of Sodium Dodecyl Sulfate polyacrylamide gel electrophoresis for protein fractionation and Sodium Dodecyl Sulfate free polyacrylamide gel electrophoresis for peptide separation as a novel procedure for proteomic studies. Here, we present an optimized device for Sodium Dodecyl Sulfate free polyacrylamide gel electrophoresis improving peptide recoveries respect to the established electrophoretic technique off gel electrophoresis meanwhile conserving the excellent resolution described for the former technique in slab gel based systems. The device simultaneously allows the separation and the collection of fractionated peptides in solution.
Annia Gonzalez - One of the best experts on this subject based on the ideXlab platform.
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Sodium Dodecyl Sulfate free gel electrophoresis/electroelution sorting for peptide fractionation
Journal of Separation Science, 2019Co-Authors: Yassel Ramos, Annia Gonzalez, Yairet Garcia, Aniel Sanchez, Patricia Sosa‐acosta, Yasset Perez-riverol, Lila Castellanos-serra, Jeovanis Gil, Luis Javier González, Vladimir BesadaAbstract:Shotgun proteomics based on peptide fractionation by using liquid chromatography has become the common procedure for proteomic studies, although in the very beginning of the field, protein separation by using electrophoresis was the main tool. Nonetheless, during the last two decades, the electrophoretic techniques for peptide mixtures fractionation have evolved as a result of relevant technological improvements. We also proposed the combination of Sodium Dodecyl Sulfate polyacrylamide gel electrophoresis for protein fractionation and Sodium Dodecyl Sulfate free polyacrylamide gel electrophoresis for peptide separation as a novel procedure for proteomic studies. Here, we present an optimized device for Sodium Dodecyl Sulfate free polyacrylamide gel electrophoresis improving peptide recoveries respect to the established electrophoretic technique off gel electrophoresis meanwhile conserving the excellent resolution described for the former technique in slab gel based systems. The device simultaneously allows the separation and the collection of fractionated peptides in solution.
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Sodium Dodecyl Sulfate free gel electrophoresis electroelution sorting for peptide fractionation
IEEE Journal of Solid-state Circuits, 2019Co-Authors: Yassel Ramos, Annia Gonzalez, Patricia Sosaacosta, Yasset Perezriverol, Yairet Garcia, Lila Castellanosserra, Aniel Sanchez, L. Gonzalez, Vladimir BesadaAbstract:Shotgun proteomics based on peptide fractionation by using liquid chromatography has become the common procedure for proteomic studies, although in the very beginning of the field, protein separation by using electrophoresis was the main tool. Nonetheless, during the last two decades, the electrophoretic techniques for peptide mixtures fractionation have evolved as a result of relevant technological improvements. We also proposed the combination of Sodium Dodecyl Sulfate polyacrylamide gel electrophoresis for protein fractionation and Sodium Dodecyl Sulfate free polyacrylamide gel electrophoresis for peptide separation as a novel procedure for proteomic studies. Here, we present an optimized device for Sodium Dodecyl Sulfate free polyacrylamide gel electrophoresis improving peptide recoveries respect to the established electrophoretic technique off gel electrophoresis meanwhile conserving the excellent resolution described for the former technique in slab gel based systems. The device simultaneously allows the separation and the collection of fractionated peptides in solution.
Yairet Garcia - One of the best experts on this subject based on the ideXlab platform.
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Sodium Dodecyl Sulfate free gel electrophoresis/electroelution sorting for peptide fractionation
Journal of Separation Science, 2019Co-Authors: Yassel Ramos, Annia Gonzalez, Yairet Garcia, Aniel Sanchez, Patricia Sosa‐acosta, Yasset Perez-riverol, Lila Castellanos-serra, Jeovanis Gil, Luis Javier González, Vladimir BesadaAbstract:Shotgun proteomics based on peptide fractionation by using liquid chromatography has become the common procedure for proteomic studies, although in the very beginning of the field, protein separation by using electrophoresis was the main tool. Nonetheless, during the last two decades, the electrophoretic techniques for peptide mixtures fractionation have evolved as a result of relevant technological improvements. We also proposed the combination of Sodium Dodecyl Sulfate polyacrylamide gel electrophoresis for protein fractionation and Sodium Dodecyl Sulfate free polyacrylamide gel electrophoresis for peptide separation as a novel procedure for proteomic studies. Here, we present an optimized device for Sodium Dodecyl Sulfate free polyacrylamide gel electrophoresis improving peptide recoveries respect to the established electrophoretic technique off gel electrophoresis meanwhile conserving the excellent resolution described for the former technique in slab gel based systems. The device simultaneously allows the separation and the collection of fractionated peptides in solution.
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Sodium Dodecyl Sulfate free gel electrophoresis electroelution sorting for peptide fractionation
IEEE Journal of Solid-state Circuits, 2019Co-Authors: Yassel Ramos, Annia Gonzalez, Patricia Sosaacosta, Yasset Perezriverol, Yairet Garcia, Lila Castellanosserra, Aniel Sanchez, L. Gonzalez, Vladimir BesadaAbstract:Shotgun proteomics based on peptide fractionation by using liquid chromatography has become the common procedure for proteomic studies, although in the very beginning of the field, protein separation by using electrophoresis was the main tool. Nonetheless, during the last two decades, the electrophoretic techniques for peptide mixtures fractionation have evolved as a result of relevant technological improvements. We also proposed the combination of Sodium Dodecyl Sulfate polyacrylamide gel electrophoresis for protein fractionation and Sodium Dodecyl Sulfate free polyacrylamide gel electrophoresis for peptide separation as a novel procedure for proteomic studies. Here, we present an optimized device for Sodium Dodecyl Sulfate free polyacrylamide gel electrophoresis improving peptide recoveries respect to the established electrophoretic technique off gel electrophoresis meanwhile conserving the excellent resolution described for the former technique in slab gel based systems. The device simultaneously allows the separation and the collection of fractionated peptides in solution.
Aniel Sanchez - One of the best experts on this subject based on the ideXlab platform.
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Sodium Dodecyl Sulfate free gel electrophoresis/electroelution sorting for peptide fractionation
Journal of Separation Science, 2019Co-Authors: Yassel Ramos, Annia Gonzalez, Yairet Garcia, Aniel Sanchez, Patricia Sosa‐acosta, Yasset Perez-riverol, Lila Castellanos-serra, Jeovanis Gil, Luis Javier González, Vladimir BesadaAbstract:Shotgun proteomics based on peptide fractionation by using liquid chromatography has become the common procedure for proteomic studies, although in the very beginning of the field, protein separation by using electrophoresis was the main tool. Nonetheless, during the last two decades, the electrophoretic techniques for peptide mixtures fractionation have evolved as a result of relevant technological improvements. We also proposed the combination of Sodium Dodecyl Sulfate polyacrylamide gel electrophoresis for protein fractionation and Sodium Dodecyl Sulfate free polyacrylamide gel electrophoresis for peptide separation as a novel procedure for proteomic studies. Here, we present an optimized device for Sodium Dodecyl Sulfate free polyacrylamide gel electrophoresis improving peptide recoveries respect to the established electrophoretic technique off gel electrophoresis meanwhile conserving the excellent resolution described for the former technique in slab gel based systems. The device simultaneously allows the separation and the collection of fractionated peptides in solution.
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Sodium Dodecyl Sulfate free gel electrophoresis electroelution sorting for peptide fractionation
IEEE Journal of Solid-state Circuits, 2019Co-Authors: Yassel Ramos, Annia Gonzalez, Patricia Sosaacosta, Yasset Perezriverol, Yairet Garcia, Lila Castellanosserra, Aniel Sanchez, L. Gonzalez, Vladimir BesadaAbstract:Shotgun proteomics based on peptide fractionation by using liquid chromatography has become the common procedure for proteomic studies, although in the very beginning of the field, protein separation by using electrophoresis was the main tool. Nonetheless, during the last two decades, the electrophoretic techniques for peptide mixtures fractionation have evolved as a result of relevant technological improvements. We also proposed the combination of Sodium Dodecyl Sulfate polyacrylamide gel electrophoresis for protein fractionation and Sodium Dodecyl Sulfate free polyacrylamide gel electrophoresis for peptide separation as a novel procedure for proteomic studies. Here, we present an optimized device for Sodium Dodecyl Sulfate free polyacrylamide gel electrophoresis improving peptide recoveries respect to the established electrophoretic technique off gel electrophoresis meanwhile conserving the excellent resolution described for the former technique in slab gel based systems. The device simultaneously allows the separation and the collection of fractionated peptides in solution.
