The Experts below are selected from a list of 1032 Experts worldwide ranked by ideXlab platform

Zhulong Chan - One of the best experts on this subject based on the ideXlab platform.

  • exogenous application of hydrogen sulfide donor Sodium Hydrosulfide enhanced multiple abiotic stress tolerance in bermudagrass cynodon dactylon l pers
    Plant Physiology and Biochemistry, 2013
    Co-Authors: Haitao Shi, Zhulong Chan
    Abstract:

    As a gaseous molecule, hydrogen sulfide (H2S) has been recently found to be involved in plant responses to multiple abiotic stress. In this study, salt (150 and 300 mM NaCl), osmotic (15% and 30% PEG6000) and cold (4 °C) stress treatments induced accumulation of endogenous H2S level, indicating that H2S might play a role in bermudagrass responses to salt, osmotic and cold stresses. Exogenous application of H2S donor (Sodium Hydrosulfide, NaHS) conferred improved salt, osmotic and freezing stress tolerances in bermudagrass, which were evidenced by decreased electrolyte leakage and increased survival rate under stress conditions. Additionally, NaHS treatment alleviated the reactive oxygen species (ROS) burst and cell damage induced by abiotic stress, via modulating metabolisms of several antioxidant enzymes [catalase (CAT), peroxidase (POD) and GR (glutathione reductase)] and non-enzymatic glutathione antioxidant pool and redox state. Moreover, exogenous NaHS treatment led to accumulation of osmolytes (proline, sucrose and soluble total sugars) in stressed bermudagrass plants. Taken together, all these data indicated the protective roles of H2S in bermudagrass responses to salt, osmotic and freezing stresses, via activation of the antioxidant response and osmolyte accumulation. These findings might be applicable to grass and crop engineering to improve abiotic stress tolerance.

  • exogenous application of hydrogen sulfide donor Sodium Hydrosulfide enhanced multiple abiotic stress tolerance in bermudagrass cynodon dactylon l pers
    Plant Physiology and Biochemistry, 2013
    Co-Authors: Tiantian Ye, Zhulong Chan
    Abstract:

    Abstract As a gaseous molecule, hydrogen sulfide (H2S) has been recently found to be involved in plant responses to multiple abiotic stress. In this study, salt (150 and 300 mM NaCl), osmotic (15% and 30% PEG6000) and cold (4 °C) stress treatments induced accumulation of endogenous H2S level, indicating that H2S might play a role in bermudagrass responses to salt, osmotic and cold stresses. Exogenous application of H2S donor (Sodium Hydrosulfide, NaHS) conferred improved salt, osmotic and freezing stress tolerances in bermudagrass, which were evidenced by decreased electrolyte leakage and increased survival rate under stress conditions. Additionally, NaHS treatment alleviated the reactive oxygen species (ROS) burst and cell damage induced by abiotic stress, via modulating metabolisms of several antioxidant enzymes [catalase (CAT), peroxidase (POD) and GR (glutathione reductase)] and non-enzymatic glutathione antioxidant pool and redox state. Moreover, exogenous NaHS treatment led to accumulation of osmolytes (proline, sucrose and soluble total sugars) in stressed bermudagrass plants. Taken together, all these data indicated the protective roles of H2S in bermudagrass responses to salt, osmotic and freezing stresses, via activation of the antioxidant response and osmolyte accumulation. These findings might be applicable to grass and crop engineering to improve abiotic stress tolerance.

Lan Yang - One of the best experts on this subject based on the ideXlab platform.

  • hydrogen sulfide donor Sodium Hydrosulfide induced heat tolerance in tobacco nicotiana tabacum l suspension cultured cells and involvement of ca2 and calmodulin
    Plant Science, 2012
    Co-Authors: Zhongguang Li, Ming Gong, Lan Yang, Jing Li
    Abstract:

    Abstract Hydrogen sulfide (H2S) is considered as a new emerging cell signal in higher plants. Hydrogen sulfide donor, Sodium Hydrosulfide, pretreatment significantly increased survival percentage of tobacco suspension cultured cells under heat stress and regrowth ability after heat stress, and alleviated decrease in vitality of cells, increase in electrolyte leakage and accumulation of malondialdehyde (MDA). In addition, Sodium Hydrosulfide-induced heat tolerance was markedly strengthened by application of exogenous Ca2+ and its ionophore A23187, respectively, while this heat tolerance was weakened by addition of Ca2+ chelator ethylene glycol-bis(b-aminoethylether)-N,N,N′,N′-tetraacetic acid (EGTA), plasma membrane channel blocker La3+, as well as calmodulin (CaM) antagonists chlorpromazine (CPZ) and trifluoperazine (TFP), respectively, but intracellular channel blocker ruthenium red (RR) did not. These results suggested that Sodium Hydrosulfide pretreatment could improve heat tolerance in tobacco suspension cultured cells and the acquisition of this heat tolerance requires the entry of extracellular Ca2+ into cells across the plasma membrane and the mediation of intracellular CaM.

  • hydrogen sulfide donor Sodium Hydrosulfide induced heat tolerance in tobacco nicotiana tabacum l suspension cultured cells and involvement of ca 2 and calmodulin
    Plant Science, 2012
    Co-Authors: Ming Gong, Hong Xie, Lan Yang
    Abstract:

    Hydrogen sulfide (H(2)S) is considered as a new emerging cell signal in higher plants. Hydrogen sulfide donor, Sodium Hydrosulfide, pretreatment significantly increased survival percentage of tobacco suspension cultured cells under heat stress and regrowth ability after heat stress, and alleviated decrease in vitality of cells, increase in electrolyte leakage and accumulation of malondialdehyde (MDA). In addition, Sodium Hydrosulfide-induced heat tolerance was markedly strengthened by application of exogenous Ca(2+) and its ionophore A23187, respectively, while this heat tolerance was weakened by addition of Ca(2+) chelator ethylene glycol-bis(b-aminoethylether)-N,N,N',N'-tetraacetic acid (EGTA), plasma membrane channel blocker La(3+), as well as calmodulin (CaM) antagonists chlorpromazine (CPZ) and trifluoperazine (TFP), respectively, but intracellular channel blocker ruthenium red (RR) did not. These results suggested that Sodium Hydrosulfide pretreatment could improve heat tolerance in tobacco suspension cultured cells and the acquisition of this heat tolerance requires the entry of extracellular Ca(2+) into cells across the plasma membrane and the mediation of intracellular CaM.

Ming Gong - One of the best experts on this subject based on the ideXlab platform.

  • hydrogen sulfide donor Sodium Hydrosulfide induced heat tolerance in tobacco nicotiana tabacum l suspension cultured cells and involvement of ca2 and calmodulin
    Plant Science, 2012
    Co-Authors: Zhongguang Li, Ming Gong, Lan Yang, Jing Li
    Abstract:

    Abstract Hydrogen sulfide (H2S) is considered as a new emerging cell signal in higher plants. Hydrogen sulfide donor, Sodium Hydrosulfide, pretreatment significantly increased survival percentage of tobacco suspension cultured cells under heat stress and regrowth ability after heat stress, and alleviated decrease in vitality of cells, increase in electrolyte leakage and accumulation of malondialdehyde (MDA). In addition, Sodium Hydrosulfide-induced heat tolerance was markedly strengthened by application of exogenous Ca2+ and its ionophore A23187, respectively, while this heat tolerance was weakened by addition of Ca2+ chelator ethylene glycol-bis(b-aminoethylether)-N,N,N′,N′-tetraacetic acid (EGTA), plasma membrane channel blocker La3+, as well as calmodulin (CaM) antagonists chlorpromazine (CPZ) and trifluoperazine (TFP), respectively, but intracellular channel blocker ruthenium red (RR) did not. These results suggested that Sodium Hydrosulfide pretreatment could improve heat tolerance in tobacco suspension cultured cells and the acquisition of this heat tolerance requires the entry of extracellular Ca2+ into cells across the plasma membrane and the mediation of intracellular CaM.

  • hydrogen sulfide donor Sodium Hydrosulfide induced heat tolerance in tobacco nicotiana tabacum l suspension cultured cells and involvement of ca 2 and calmodulin
    Plant Science, 2012
    Co-Authors: Ming Gong, Hong Xie, Lan Yang
    Abstract:

    Hydrogen sulfide (H(2)S) is considered as a new emerging cell signal in higher plants. Hydrogen sulfide donor, Sodium Hydrosulfide, pretreatment significantly increased survival percentage of tobacco suspension cultured cells under heat stress and regrowth ability after heat stress, and alleviated decrease in vitality of cells, increase in electrolyte leakage and accumulation of malondialdehyde (MDA). In addition, Sodium Hydrosulfide-induced heat tolerance was markedly strengthened by application of exogenous Ca(2+) and its ionophore A23187, respectively, while this heat tolerance was weakened by addition of Ca(2+) chelator ethylene glycol-bis(b-aminoethylether)-N,N,N',N'-tetraacetic acid (EGTA), plasma membrane channel blocker La(3+), as well as calmodulin (CaM) antagonists chlorpromazine (CPZ) and trifluoperazine (TFP), respectively, but intracellular channel blocker ruthenium red (RR) did not. These results suggested that Sodium Hydrosulfide pretreatment could improve heat tolerance in tobacco suspension cultured cells and the acquisition of this heat tolerance requires the entry of extracellular Ca(2+) into cells across the plasma membrane and the mediation of intracellular CaM.

Zhongguang Li - One of the best experts on this subject based on the ideXlab platform.

  • effect of pretreatment with hydrogen sulfide donor Sodium Hydrosulfide on heat tolerance in relation to antioxidant system in maize zea mays seedlings
    Biologia, 2014
    Co-Authors: Zhongguang Li, Xiaoyun Yi, Yuting Li
    Abstract:

    Hydrogen sulfide (H2S) is a signal molecule that is involved in plant growth, development and the acquisition of stress tolerance including heat tolerance, but the mechanism of H2S-induced heat tolerance is not completely clear. In present study, the effect of Sodium Hydrosulfide (NaHS), a H2S donor, treatment on heat tolerance of maize seedlings in relation to antioxidant system was investigated. The results showed that NaHS treatment improved survival percentage of maize seedlings under heat stress in a concentration-dependent manner, indicating that H2 St reatment could improve heat tolerance of maize seedlings. To further study mechanism of NaHS-induced heat tolerance, catalase (CAT), guaiacol peroxidase (GPX), superoxide dismutase (SOD), glutathione reductase (GR) and ascorbate peroxidase (APX) activities, and glutathione (GSH) and ascorbic acid (AsA) contents in maize seedlings were determined. The results showed that NaHS treatment increased the activities of CAT, GPX, SOD and GR, and GSH and AsA contents as well as the ratio of reduced antioxidants to total antioxidants (AsA/(AsA+DHA) and GSH/(GSH +GSSG)) in maize seedlings under normal culture conditions compared with the control. Under heat stress, antioxidant enzymes activities, antioxidants contents and the ratio of the reduced antioxidants to total antioxidants in control and treated seedlings all decreased, but NaHS-treated seedlings maintained higher antioxidant enzymes activities and antioxidants levels as well as the ratio of reduced antioxidants to total antioxidants. All of above-mentioned results suggested that NaHS treatment could improve heat tolerance of maize seedlings, and the acquisition of this heat tolerance may be relation to enhanced antioxidant system activity.

  • hydrogen sulfide donor Sodium Hydrosulfide improved heat tolerance in maize and involvement of proline
    Journal of Plant Physiology, 2013
    Co-Authors: Zhongguang Li, Xiaojiao Ding, Peifang Du
    Abstract:

    Hydrogen sulfide (H2S) has long been considered as a phytotoxin, but nowadays as a cell signal molecule involved in growth, development, and the acquisition of stress tolerance in higher plants. In the present study, hydrogen sulfide donor, Sodium Hydrosulfide (NaHS), pretreatment markedly improved germination percentage of seeds and survival percentage of seedlings of maize under heat stress, and alleviated an increase in electrolyte leakage of roots, a decrease in tissue vitality and an accumulation of malondialdehyde (MDA) in coleoptiles of maize seedlings. In addition, pretreatment of NaHS could improve the activity of Δ1-pyrroline-5-carboxylate synthetase (P5CS) and lower proline dehydrogenase (ProDH) activity, which in turn induced accumulation of endogenous proline in maize seedlings. Also, application of proline could enhance endogenous proline content, followed by mitigated accumulation of MDA and increased survival percentage of maize seedlings under heat stress. These results suggest that Sodium Hydrosulfide pretreatment could improve heat tolerance of maize and the acquisition of this heat tolerance may be involved in proline.

  • hydrogen sulfide donor Sodium Hydrosulfide induced heat tolerance in tobacco nicotiana tabacum l suspension cultured cells and involvement of ca2 and calmodulin
    Plant Science, 2012
    Co-Authors: Zhongguang Li, Ming Gong, Lan Yang, Jing Li
    Abstract:

    Abstract Hydrogen sulfide (H2S) is considered as a new emerging cell signal in higher plants. Hydrogen sulfide donor, Sodium Hydrosulfide, pretreatment significantly increased survival percentage of tobacco suspension cultured cells under heat stress and regrowth ability after heat stress, and alleviated decrease in vitality of cells, increase in electrolyte leakage and accumulation of malondialdehyde (MDA). In addition, Sodium Hydrosulfide-induced heat tolerance was markedly strengthened by application of exogenous Ca2+ and its ionophore A23187, respectively, while this heat tolerance was weakened by addition of Ca2+ chelator ethylene glycol-bis(b-aminoethylether)-N,N,N′,N′-tetraacetic acid (EGTA), plasma membrane channel blocker La3+, as well as calmodulin (CaM) antagonists chlorpromazine (CPZ) and trifluoperazine (TFP), respectively, but intracellular channel blocker ruthenium red (RR) did not. These results suggested that Sodium Hydrosulfide pretreatment could improve heat tolerance in tobacco suspension cultured cells and the acquisition of this heat tolerance requires the entry of extracellular Ca2+ into cells across the plasma membrane and the mediation of intracellular CaM.

Jing Li - One of the best experts on this subject based on the ideXlab platform.

  • hydrogen sulfide donor Sodium Hydrosulfide induced heat tolerance in tobacco nicotiana tabacum l suspension cultured cells and involvement of ca2 and calmodulin
    Plant Science, 2012
    Co-Authors: Zhongguang Li, Ming Gong, Lan Yang, Jing Li
    Abstract:

    Abstract Hydrogen sulfide (H2S) is considered as a new emerging cell signal in higher plants. Hydrogen sulfide donor, Sodium Hydrosulfide, pretreatment significantly increased survival percentage of tobacco suspension cultured cells under heat stress and regrowth ability after heat stress, and alleviated decrease in vitality of cells, increase in electrolyte leakage and accumulation of malondialdehyde (MDA). In addition, Sodium Hydrosulfide-induced heat tolerance was markedly strengthened by application of exogenous Ca2+ and its ionophore A23187, respectively, while this heat tolerance was weakened by addition of Ca2+ chelator ethylene glycol-bis(b-aminoethylether)-N,N,N′,N′-tetraacetic acid (EGTA), plasma membrane channel blocker La3+, as well as calmodulin (CaM) antagonists chlorpromazine (CPZ) and trifluoperazine (TFP), respectively, but intracellular channel blocker ruthenium red (RR) did not. These results suggested that Sodium Hydrosulfide pretreatment could improve heat tolerance in tobacco suspension cultured cells and the acquisition of this heat tolerance requires the entry of extracellular Ca2+ into cells across the plasma membrane and the mediation of intracellular CaM.