The Experts below are selected from a list of 66 Experts worldwide ranked by ideXlab platform

Sung Hyun Kim - One of the best experts on this subject based on the ideXlab platform.

  • use of rt qpcr with combined intercalating dye and Sodium Lauroyl Sarcosinate pretreatment to evaluate the virucidal activity of halophyte extracts against norovirus
    Food Control, 2019
    Co-Authors: Haewon Lee, Sora Yoon, Heemin Lee, Jae Young Lee, Sung Hyun Kim
    Abstract:

    Abstract In this study, we evaluated the virucidal effects of chloroform, ethyl acetate, and n-butanol soluble fractions of ethanolic halophyte extracts, including extracts of iceplant (Mesembryantchemum crystallinum L.), salt sandspurry (Spergularia marina L. Besser), beach silvertop (Glehnia littoralis F. Schmidt ex Miq.), marshfire glasswort (Salicornia europaea L.), and East Asian seepweed (Suaeda japonica Makino), against human norovirus (HuNoV). Using reverse transcription quantitative polymerase chain reaction, magnetic bead separation for enrichment of viral particles with pretreatment combining propidium monoazide and Sodium Lauroyl Sarcosinate, we found that the 100 μg/mL n-butanol fractions from S. europaea and S. japonica extracts showed the highest virucidal effects against HuNoV GII.4 after 1 h, as shown by log10 reductions of more than 2 (P

  • pretreatment with propidium monoazide Sodium Lauroyl Sarcosinate improves discrimination of infectious waterborne virus by rt qpcr combined with magnetic separation
    Environmental Pollution, 2018
    Co-Authors: Haewon Lee, Sora Yoon, Heemin Lee, Sung Hyun Kim
    Abstract:

    RT-qPCR allows sensitive detection of viral particles of both infectious and noninfectious viruses in water environments, but cannot discriminate non-infectious from infectious viruses. In this study, we aimed to optimize RT-qPCR-based detection of chlorine-inactivated human norovirus (NoV) and pepper mild mottle virus (PMMoV) in suspension by pretreatment with an optimal combination of a monoazide and a detergent that can efficiently penetrate damaged viral capsids. Four methods were compared to determine the efficacy of chlorine disinfection (at 1, 3, and 5 min mg/L): (A) RT-qPCR alone, (B) RT-qPCR assay preceded by magnetic bead separation for enrichment of viral particles (MBS-RT-qPCR), (C) MBS-RT-qPCR assay with pretreatment with propidium monoazide (PMA-MBS-RT-qPCR), and (D) PMA-MBS-RT-qPCR assay with pretreatment with Sodium Lauroyl Sarcosinate (INCI-PMA-MBS-RT-qPCR). On the basis of a PMA optimization assay, 200 and 300 μM PMA were used in subsequent experiments for NoV GII.4 and PMMoV, respectively. Optimal INCI concentrations, having minimal influence on NoV GII.4 and PMMoV, were found to be 0.5% and 0.2% INCI, respectively. For NoV GII.4, there were significant differences (P < 0.05) in log10 genome copies between the PMA-treated and the INCI + PMA-treated samples (log10 genome copies differed by 1.11 and 0.59 log10 for 3 and 5 min mg/L of chlorine, respectively). For PMMoV, INCI induced differences in log10 genome copies of 0.92, 1.18, and 1.86, for 1, 3, and 5 min mg/L of chlorine, respectively. Overall, the results of this study indicate that an optimal combination of PMA and INCI could be very useful for evaluating disinfection methods in water treatment strategies.

Haewon Lee - One of the best experts on this subject based on the ideXlab platform.

  • use of rt qpcr with combined intercalating dye and Sodium Lauroyl Sarcosinate pretreatment to evaluate the virucidal activity of halophyte extracts against norovirus
    Food Control, 2019
    Co-Authors: Haewon Lee, Sora Yoon, Heemin Lee, Jae Young Lee, Sung Hyun Kim
    Abstract:

    Abstract In this study, we evaluated the virucidal effects of chloroform, ethyl acetate, and n-butanol soluble fractions of ethanolic halophyte extracts, including extracts of iceplant (Mesembryantchemum crystallinum L.), salt sandspurry (Spergularia marina L. Besser), beach silvertop (Glehnia littoralis F. Schmidt ex Miq.), marshfire glasswort (Salicornia europaea L.), and East Asian seepweed (Suaeda japonica Makino), against human norovirus (HuNoV). Using reverse transcription quantitative polymerase chain reaction, magnetic bead separation for enrichment of viral particles with pretreatment combining propidium monoazide and Sodium Lauroyl Sarcosinate, we found that the 100 μg/mL n-butanol fractions from S. europaea and S. japonica extracts showed the highest virucidal effects against HuNoV GII.4 after 1 h, as shown by log10 reductions of more than 2 (P

  • pretreatment with propidium monoazide Sodium Lauroyl Sarcosinate improves discrimination of infectious waterborne virus by rt qpcr combined with magnetic separation
    Environmental Pollution, 2018
    Co-Authors: Haewon Lee, Sora Yoon, Heemin Lee, Sung Hyun Kim
    Abstract:

    RT-qPCR allows sensitive detection of viral particles of both infectious and noninfectious viruses in water environments, but cannot discriminate non-infectious from infectious viruses. In this study, we aimed to optimize RT-qPCR-based detection of chlorine-inactivated human norovirus (NoV) and pepper mild mottle virus (PMMoV) in suspension by pretreatment with an optimal combination of a monoazide and a detergent that can efficiently penetrate damaged viral capsids. Four methods were compared to determine the efficacy of chlorine disinfection (at 1, 3, and 5 min mg/L): (A) RT-qPCR alone, (B) RT-qPCR assay preceded by magnetic bead separation for enrichment of viral particles (MBS-RT-qPCR), (C) MBS-RT-qPCR assay with pretreatment with propidium monoazide (PMA-MBS-RT-qPCR), and (D) PMA-MBS-RT-qPCR assay with pretreatment with Sodium Lauroyl Sarcosinate (INCI-PMA-MBS-RT-qPCR). On the basis of a PMA optimization assay, 200 and 300 μM PMA were used in subsequent experiments for NoV GII.4 and PMMoV, respectively. Optimal INCI concentrations, having minimal influence on NoV GII.4 and PMMoV, were found to be 0.5% and 0.2% INCI, respectively. For NoV GII.4, there were significant differences (P < 0.05) in log10 genome copies between the PMA-treated and the INCI + PMA-treated samples (log10 genome copies differed by 1.11 and 0.59 log10 for 3 and 5 min mg/L of chlorine, respectively). For PMMoV, INCI induced differences in log10 genome copies of 0.92, 1.18, and 1.86, for 1, 3, and 5 min mg/L of chlorine, respectively. Overall, the results of this study indicate that an optimal combination of PMA and INCI could be very useful for evaluating disinfection methods in water treatment strategies.

Heemin Lee - One of the best experts on this subject based on the ideXlab platform.

  • use of rt qpcr with combined intercalating dye and Sodium Lauroyl Sarcosinate pretreatment to evaluate the virucidal activity of halophyte extracts against norovirus
    Food Control, 2019
    Co-Authors: Haewon Lee, Sora Yoon, Heemin Lee, Jae Young Lee, Sung Hyun Kim
    Abstract:

    Abstract In this study, we evaluated the virucidal effects of chloroform, ethyl acetate, and n-butanol soluble fractions of ethanolic halophyte extracts, including extracts of iceplant (Mesembryantchemum crystallinum L.), salt sandspurry (Spergularia marina L. Besser), beach silvertop (Glehnia littoralis F. Schmidt ex Miq.), marshfire glasswort (Salicornia europaea L.), and East Asian seepweed (Suaeda japonica Makino), against human norovirus (HuNoV). Using reverse transcription quantitative polymerase chain reaction, magnetic bead separation for enrichment of viral particles with pretreatment combining propidium monoazide and Sodium Lauroyl Sarcosinate, we found that the 100 μg/mL n-butanol fractions from S. europaea and S. japonica extracts showed the highest virucidal effects against HuNoV GII.4 after 1 h, as shown by log10 reductions of more than 2 (P

  • pretreatment with propidium monoazide Sodium Lauroyl Sarcosinate improves discrimination of infectious waterborne virus by rt qpcr combined with magnetic separation
    Environmental Pollution, 2018
    Co-Authors: Haewon Lee, Sora Yoon, Heemin Lee, Sung Hyun Kim
    Abstract:

    RT-qPCR allows sensitive detection of viral particles of both infectious and noninfectious viruses in water environments, but cannot discriminate non-infectious from infectious viruses. In this study, we aimed to optimize RT-qPCR-based detection of chlorine-inactivated human norovirus (NoV) and pepper mild mottle virus (PMMoV) in suspension by pretreatment with an optimal combination of a monoazide and a detergent that can efficiently penetrate damaged viral capsids. Four methods were compared to determine the efficacy of chlorine disinfection (at 1, 3, and 5 min mg/L): (A) RT-qPCR alone, (B) RT-qPCR assay preceded by magnetic bead separation for enrichment of viral particles (MBS-RT-qPCR), (C) MBS-RT-qPCR assay with pretreatment with propidium monoazide (PMA-MBS-RT-qPCR), and (D) PMA-MBS-RT-qPCR assay with pretreatment with Sodium Lauroyl Sarcosinate (INCI-PMA-MBS-RT-qPCR). On the basis of a PMA optimization assay, 200 and 300 μM PMA were used in subsequent experiments for NoV GII.4 and PMMoV, respectively. Optimal INCI concentrations, having minimal influence on NoV GII.4 and PMMoV, were found to be 0.5% and 0.2% INCI, respectively. For NoV GII.4, there were significant differences (P < 0.05) in log10 genome copies between the PMA-treated and the INCI + PMA-treated samples (log10 genome copies differed by 1.11 and 0.59 log10 for 3 and 5 min mg/L of chlorine, respectively). For PMMoV, INCI induced differences in log10 genome copies of 0.92, 1.18, and 1.86, for 1, 3, and 5 min mg/L of chlorine, respectively. Overall, the results of this study indicate that an optimal combination of PMA and INCI could be very useful for evaluating disinfection methods in water treatment strategies.

Sora Yoon - One of the best experts on this subject based on the ideXlab platform.

  • use of rt qpcr with combined intercalating dye and Sodium Lauroyl Sarcosinate pretreatment to evaluate the virucidal activity of halophyte extracts against norovirus
    Food Control, 2019
    Co-Authors: Haewon Lee, Sora Yoon, Heemin Lee, Jae Young Lee, Sung Hyun Kim
    Abstract:

    Abstract In this study, we evaluated the virucidal effects of chloroform, ethyl acetate, and n-butanol soluble fractions of ethanolic halophyte extracts, including extracts of iceplant (Mesembryantchemum crystallinum L.), salt sandspurry (Spergularia marina L. Besser), beach silvertop (Glehnia littoralis F. Schmidt ex Miq.), marshfire glasswort (Salicornia europaea L.), and East Asian seepweed (Suaeda japonica Makino), against human norovirus (HuNoV). Using reverse transcription quantitative polymerase chain reaction, magnetic bead separation for enrichment of viral particles with pretreatment combining propidium monoazide and Sodium Lauroyl Sarcosinate, we found that the 100 μg/mL n-butanol fractions from S. europaea and S. japonica extracts showed the highest virucidal effects against HuNoV GII.4 after 1 h, as shown by log10 reductions of more than 2 (P

  • pretreatment with propidium monoazide Sodium Lauroyl Sarcosinate improves discrimination of infectious waterborne virus by rt qpcr combined with magnetic separation
    Environmental Pollution, 2018
    Co-Authors: Haewon Lee, Sora Yoon, Heemin Lee, Sung Hyun Kim
    Abstract:

    RT-qPCR allows sensitive detection of viral particles of both infectious and noninfectious viruses in water environments, but cannot discriminate non-infectious from infectious viruses. In this study, we aimed to optimize RT-qPCR-based detection of chlorine-inactivated human norovirus (NoV) and pepper mild mottle virus (PMMoV) in suspension by pretreatment with an optimal combination of a monoazide and a detergent that can efficiently penetrate damaged viral capsids. Four methods were compared to determine the efficacy of chlorine disinfection (at 1, 3, and 5 min mg/L): (A) RT-qPCR alone, (B) RT-qPCR assay preceded by magnetic bead separation for enrichment of viral particles (MBS-RT-qPCR), (C) MBS-RT-qPCR assay with pretreatment with propidium monoazide (PMA-MBS-RT-qPCR), and (D) PMA-MBS-RT-qPCR assay with pretreatment with Sodium Lauroyl Sarcosinate (INCI-PMA-MBS-RT-qPCR). On the basis of a PMA optimization assay, 200 and 300 μM PMA were used in subsequent experiments for NoV GII.4 and PMMoV, respectively. Optimal INCI concentrations, having minimal influence on NoV GII.4 and PMMoV, were found to be 0.5% and 0.2% INCI, respectively. For NoV GII.4, there were significant differences (P < 0.05) in log10 genome copies between the PMA-treated and the INCI + PMA-treated samples (log10 genome copies differed by 1.11 and 0.59 log10 for 3 and 5 min mg/L of chlorine, respectively). For PMMoV, INCI induced differences in log10 genome copies of 0.92, 1.18, and 1.86, for 1, 3, and 5 min mg/L of chlorine, respectively. Overall, the results of this study indicate that an optimal combination of PMA and INCI could be very useful for evaluating disinfection methods in water treatment strategies.

Tong Lin - One of the best experts on this subject based on the ideXlab platform.