The Experts below are selected from a list of 20034 Experts worldwide ranked by ideXlab platform
Hans-christoph Pape - One of the best experts on this subject based on the ideXlab platform.
-
Cumulative Effects of Bone and Soft Tissue Injury on Systemic Inflammation: A Pilot Study
Clinical Orthopaedics and Related Research®, 2013Co-Authors: Roman Pfeifer, Sophie Darwiche, Lauryn Kohut, Timothy R. Billiar, Hans-christoph PapeAbstract:Background In multiply injured patients, bilateral femur fractures invoke a substantial systemic inflammatory impact and remote organ dysfunction. However, it is unclear whether isolated bone or Soft Tissue Injury contributes to the systemic inflammatory response and organ Injury after fracture. Questions/purposes We therefore asked whether the systemic inflammatory response and remote organ dysfunction are attributable to the bone fragment injection, adjacent Soft Tissue Injury, or both. Methods Male C57/BL6 mice (8–10 weeks old, 20–30 g) were assigned to four groups: bone fragment injection (BF, n = 9) group; Soft Tissue Injury (STI, n = 9) group; BF + STI (n = 9) group, in which both insults were applied; and control group, in which neither insult was applied. Animals were sacrificed at 6 hours. As surrogates for systemic inflammation, we measured serum IL-6, IL-10, osteopontin, and alanine aminotransferase (ALT) and nuclear factor (NF)-κB and myeloperoxidase (MPO) in the lung. Results The systemic inflammatory response (mean IL-6 level) was similar in the BF (61.8 pg/mL) and STI (67.9 pg/mL) groups. The combination (BF + STI) of both traumatic insults induced an increase in mean levels of inflammatory parameters (IL-6: 189.1 pg/mL) but not in MPO levels (1.21 ng/mL) as compared with the BF (0.82 ng/mL) and STI (1.26 ng/mL) groups. The model produced little evidence of remote organ inflammation. Conclusions Our findings suggest both bone and Soft Tissue Injury are required to induce systemic changes. The absence of remote organ inflammation suggests further fracture-associated factors, such as hemorrhage and fat liberation, may be more critical for induction of remote organ damage. Clinical Relevance Both bone and Soft Tissue injuries contribute to the systemic inflammatory response.
-
cumulative effects of bone and Soft Tissue Injury on systemic inflammation a pilot study
Clinical Orthopaedics and Related Research, 2013Co-Authors: Roman Pfeifer, Sophie Darwiche, Lauryn Kohut, Timothy R. Billiar, Hans-christoph PapeAbstract:Background In multiply injured patients, bilateral femur fractures invoke a substantial systemic inflammatory impact and remote organ dysfunction. However, it is unclear whether isolated bone or Soft Tissue Injury contributes to the systemic inflammatory response and organ Injury after fracture.
-
the role of fracture associated Soft Tissue Injury in the induction of systemic inflammation and remote organ dysfunction after bilateral femur fracture
Journal of Orthopaedic Trauma, 2008Co-Authors: Philipp Kobbe, Timothy R. Billiar, Yoram Vodovotz, David J Kaczorowski, Hans-christoph PapeAbstract:Objectives:The storage of preformed cytokines in Soft Tissue as well as the immunocompetence of adipocytes has been reported. We hypothesized that fracture-associated Soft Tissue Injury plays a pivotal role in the induction of systemic inflammation and remote organ dysfunction after fracture.Materia
Shane M Heffernan - One of the best experts on this subject based on the ideXlab platform.
-
col5a1 gene variants previously associated with reduced Soft Tissue Injury risk are associated with elite athlete status in rugby
BMC Genomics, 2017Co-Authors: Stuart M Raleigh, Shane M Heffernan, Liam P Kilduff, Georgina K Stebbings, Christian J. Cook, Robert M. Erskine, Mark A. BennettAbstract:Two common single nucleotide polymorphisms within the COL5A1 gene (SNPs; rs12722 C/T and rs3196378 C/A) have previously been associated with tendon and ligament pathologies. Given the high incidence of tendon and ligament injuries in elite rugby athletes, we hypothesised that both SNPs would be associated with career success. In 1105 participants (RugbyGene project), comprising 460 elite rugby union (RU), 88 elite rugby league athletes and 565 non-athlete controls, DNA was collected and genotyped for the COL5A1 rs12722 and rs3196378 variants using real-time PCR. For rs12722, the Injury-protective CC genotype and C allele were more common in all athletes (21% and 47%, respectively) and RU athletes (22% and 48%) than in controls (16% and 41%, P ≤ 0.01). For rs3196378, the CC genotype and C allele were overrepresented in all athletes (23% and 48%) and RU athletes (24% and 49%) compared with controls (16% and 41%, P ≤ 0.02). The CC genotype in particular was overrepresented in the back and centres (24%) compared with controls, with more than twice the odds (OR = 2.25, P = 0.006) of possessing the Injury-protective CC genotype. Furthermore, when considering both SNPs simultaneously, the CC–CC SNP-SNP combination and C–C inferred allele combination were higher in all the athlete groups (≥18% and ≥43%) compared with controls (13% and 40%; P = 0.01). However, no genotype differences were identified for either SNP when RU playing positions were compared directly with each other. It appears that the C alleles, CC genotypes and resulting combinations of both rs12722 and rs3196378 are beneficial for rugby athletes to achieve elite status and carriage of these variants may impart an inherited resistance against Soft Tissue Injury, despite exposure to the high-risk environment of elite rugby. These data have implications for the management of inter-individual differences in Injury risk amongst elite athletes.
-
COL5A1 gene variants previously associated with reduced Soft Tissue Injury risk are associated with elite athlete status in rugby
BMC Genomics, 2017Co-Authors: Shane M Heffernan, Mark A. Bennett, Stuart M Raleigh, Liam P Kilduff, Georgina K Stebbings, Christian J. Cook, Robert M. Erskine, Guan Wang, Malcolm Collins, Yannis P. PitsiladisAbstract:Background Two common single nucleotide polymorphisms within the COL5A1 gene (SNPs; rs12722 C/T and rs3196378 C/A) have previously been associated with tendon and ligament pathologies. Given the high incidence of tendon and ligament injuries in elite rugby athletes, we hypothesised that both SNPs would be associated with career success. Results In 1105 participants (RugbyGene project), comprising 460 elite rugby union (RU), 88 elite rugby league athletes and 565 non-athlete controls, DNA was collected and genotyped for the COL5A1 rs12722 and rs3196378 variants using real-time PCR. For rs12722, the Injury-protective CC genotype and C allele were more common in all athletes (21% and 47%, respectively) and RU athletes (22% and 48%) than in controls (16% and 41%, P ≤ 0.01). For rs3196378, the CC genotype and C allele were overrepresented in all athletes (23% and 48%) and RU athletes (24% and 49%) compared with controls (16% and 41%, P ≤ 0.02). The CC genotype in particular was overrepresented in the back and centres (24%) compared with controls, with more than twice the odds (OR = 2.25, P = 0.006) of possessing the Injury-protective CC genotype. Furthermore, when considering both SNPs simultaneously, the CC–CC SNP-SNP combination and C–C inferred allele combination were higher in all the athlete groups (≥18% and ≥43%) compared with controls (13% and 40%; P = 0.01). However, no genotype differences were identified for either SNP when RU playing positions were compared directly with each other. Conclusion It appears that the C alleles, CC genotypes and resulting combinations of both rs12722 and rs3196378 are beneficial for rugby athletes to achieve elite status and carriage of these variants may impart an inherited resistance against Soft Tissue Injury, despite exposure to the high-risk environment of elite rugby. These data have implications for the management of inter-individual differences in Injury risk amongst elite athletes.
Ellen J. Mackenzie - One of the best experts on this subject based on the ideXlab platform.
-
proximal tibial metaphyseal fractures with severe Soft Tissue Injury clinical and functional results at 2 years
Clinical Orthopaedics and Related Research, 2010Co-Authors: James S Starman, Renan C. Castillo, Michael J. Bosse, Ellen J. MackenzieAbstract:Background Controversy exists regarding management of proximal tibial metaphyseal fractures with severe Soft Tissue Injury. It is unclear whether limb salvage or early amputation results in the best functional and clinical outcomes.
-
Proximal Tibial Metaphyseal Fractures with Severe Soft Tissue Injury
2010Co-Authors: Ellen J. Mackenzie, Michael J. Bosse, Andrew R. Burgess, Renan C. Castillo, Alan L. Jones, James F. Kellam, Mark P. Mcandrew, Brendan M. Patterson, Roy Sanders, Adam J. StarrAbstract:Background Controversy exists regarding management of proximal tibial metaphyseal fractures with severe Soft Tissue Injury. It is unclear whether limb salvage or early amputation results in the best functional and clinical outcomes. Questions/Purpose We hypothesized that in this group of patients, there is no difference in functional outcomes, complication rates, clinical outcomes, or objective physical function related to the treatment approach. Methods We used the LEAP study database to perform a retrospective comparative review of a subset of patients with proximal tibial metaphyseal fractures (AO/OTA 41A, B, and C) with associated severe Soft Tissue injuries comparing the outcomes of patients who were treated with either limb salvage or amputation. Results Although there were major differences in clinical and functional outcomes based on patients’ sociodemographics at 2 years, no differences in clinical or functional outcomes were detected regardless of whether amputation or limb salvage was performed. Severity of Soft Tissue Injury was more predictive of outcome than the surgical approach used. Conclusions Sociodemographics and Soft Tissue Injury severity are more important than treatment approach for predicting clinical and functional outcomes at 2 years in patients with proximal tibia metaphyseal fractures with severe Soft Tissue Injury. Level of Evidence Level III, retrospective comparative study. See Guidelines for Authors for a complete description of levels of evidence.
Roman Pfeifer - One of the best experts on this subject based on the ideXlab platform.
-
Cumulative Effects of Bone and Soft Tissue Injury on Systemic Inflammation: A Pilot Study
Clinical Orthopaedics and Related Research®, 2013Co-Authors: Roman Pfeifer, Sophie Darwiche, Lauryn Kohut, Timothy R. Billiar, Hans-christoph PapeAbstract:Background In multiply injured patients, bilateral femur fractures invoke a substantial systemic inflammatory impact and remote organ dysfunction. However, it is unclear whether isolated bone or Soft Tissue Injury contributes to the systemic inflammatory response and organ Injury after fracture. Questions/purposes We therefore asked whether the systemic inflammatory response and remote organ dysfunction are attributable to the bone fragment injection, adjacent Soft Tissue Injury, or both. Methods Male C57/BL6 mice (8–10 weeks old, 20–30 g) were assigned to four groups: bone fragment injection (BF, n = 9) group; Soft Tissue Injury (STI, n = 9) group; BF + STI (n = 9) group, in which both insults were applied; and control group, in which neither insult was applied. Animals were sacrificed at 6 hours. As surrogates for systemic inflammation, we measured serum IL-6, IL-10, osteopontin, and alanine aminotransferase (ALT) and nuclear factor (NF)-κB and myeloperoxidase (MPO) in the lung. Results The systemic inflammatory response (mean IL-6 level) was similar in the BF (61.8 pg/mL) and STI (67.9 pg/mL) groups. The combination (BF + STI) of both traumatic insults induced an increase in mean levels of inflammatory parameters (IL-6: 189.1 pg/mL) but not in MPO levels (1.21 ng/mL) as compared with the BF (0.82 ng/mL) and STI (1.26 ng/mL) groups. The model produced little evidence of remote organ inflammation. Conclusions Our findings suggest both bone and Soft Tissue Injury are required to induce systemic changes. The absence of remote organ inflammation suggests further fracture-associated factors, such as hemorrhage and fat liberation, may be more critical for induction of remote organ damage. Clinical Relevance Both bone and Soft Tissue injuries contribute to the systemic inflammatory response.
-
cumulative effects of bone and Soft Tissue Injury on systemic inflammation a pilot study
Clinical Orthopaedics and Related Research, 2013Co-Authors: Roman Pfeifer, Sophie Darwiche, Lauryn Kohut, Timothy R. Billiar, Hans-christoph PapeAbstract:Background In multiply injured patients, bilateral femur fractures invoke a substantial systemic inflammatory impact and remote organ dysfunction. However, it is unclear whether isolated bone or Soft Tissue Injury contributes to the systemic inflammatory response and organ Injury after fracture.
Mark A. Bennett - One of the best experts on this subject based on the ideXlab platform.
-
col5a1 gene variants previously associated with reduced Soft Tissue Injury risk are associated with elite athlete status in rugby
BMC Genomics, 2017Co-Authors: Stuart M Raleigh, Shane M Heffernan, Liam P Kilduff, Georgina K Stebbings, Christian J. Cook, Robert M. Erskine, Mark A. BennettAbstract:Two common single nucleotide polymorphisms within the COL5A1 gene (SNPs; rs12722 C/T and rs3196378 C/A) have previously been associated with tendon and ligament pathologies. Given the high incidence of tendon and ligament injuries in elite rugby athletes, we hypothesised that both SNPs would be associated with career success. In 1105 participants (RugbyGene project), comprising 460 elite rugby union (RU), 88 elite rugby league athletes and 565 non-athlete controls, DNA was collected and genotyped for the COL5A1 rs12722 and rs3196378 variants using real-time PCR. For rs12722, the Injury-protective CC genotype and C allele were more common in all athletes (21% and 47%, respectively) and RU athletes (22% and 48%) than in controls (16% and 41%, P ≤ 0.01). For rs3196378, the CC genotype and C allele were overrepresented in all athletes (23% and 48%) and RU athletes (24% and 49%) compared with controls (16% and 41%, P ≤ 0.02). The CC genotype in particular was overrepresented in the back and centres (24%) compared with controls, with more than twice the odds (OR = 2.25, P = 0.006) of possessing the Injury-protective CC genotype. Furthermore, when considering both SNPs simultaneously, the CC–CC SNP-SNP combination and C–C inferred allele combination were higher in all the athlete groups (≥18% and ≥43%) compared with controls (13% and 40%; P = 0.01). However, no genotype differences were identified for either SNP when RU playing positions were compared directly with each other. It appears that the C alleles, CC genotypes and resulting combinations of both rs12722 and rs3196378 are beneficial for rugby athletes to achieve elite status and carriage of these variants may impart an inherited resistance against Soft Tissue Injury, despite exposure to the high-risk environment of elite rugby. These data have implications for the management of inter-individual differences in Injury risk amongst elite athletes.
-
COL5A1 gene variants previously associated with reduced Soft Tissue Injury risk are associated with elite athlete status in rugby
BMC Genomics, 2017Co-Authors: Shane M Heffernan, Mark A. Bennett, Stuart M Raleigh, Liam P Kilduff, Georgina K Stebbings, Christian J. Cook, Robert M. Erskine, Guan Wang, Malcolm Collins, Yannis P. PitsiladisAbstract:Background Two common single nucleotide polymorphisms within the COL5A1 gene (SNPs; rs12722 C/T and rs3196378 C/A) have previously been associated with tendon and ligament pathologies. Given the high incidence of tendon and ligament injuries in elite rugby athletes, we hypothesised that both SNPs would be associated with career success. Results In 1105 participants (RugbyGene project), comprising 460 elite rugby union (RU), 88 elite rugby league athletes and 565 non-athlete controls, DNA was collected and genotyped for the COL5A1 rs12722 and rs3196378 variants using real-time PCR. For rs12722, the Injury-protective CC genotype and C allele were more common in all athletes (21% and 47%, respectively) and RU athletes (22% and 48%) than in controls (16% and 41%, P ≤ 0.01). For rs3196378, the CC genotype and C allele were overrepresented in all athletes (23% and 48%) and RU athletes (24% and 49%) compared with controls (16% and 41%, P ≤ 0.02). The CC genotype in particular was overrepresented in the back and centres (24%) compared with controls, with more than twice the odds (OR = 2.25, P = 0.006) of possessing the Injury-protective CC genotype. Furthermore, when considering both SNPs simultaneously, the CC–CC SNP-SNP combination and C–C inferred allele combination were higher in all the athlete groups (≥18% and ≥43%) compared with controls (13% and 40%; P = 0.01). However, no genotype differences were identified for either SNP when RU playing positions were compared directly with each other. Conclusion It appears that the C alleles, CC genotypes and resulting combinations of both rs12722 and rs3196378 are beneficial for rugby athletes to achieve elite status and carriage of these variants may impart an inherited resistance against Soft Tissue Injury, despite exposure to the high-risk environment of elite rugby. These data have implications for the management of inter-individual differences in Injury risk amongst elite athletes.
