The Experts below are selected from a list of 252 Experts worldwide ranked by ideXlab platform
Yuwen Zhuang - One of the best experts on this subject based on the ideXlab platform.
-
Solasodine reverses stemness and epithelial mesenchymal transition in human colorectal cancer
Biochemical and Biophysical Research Communications, 2018Co-Authors: Yuwen Zhuang, Cunen Wu, Jinyong Zhou, Zhiming Zhao, Junyi ShenAbstract:Abstract Adverse side effects of conventional chemotherapy, acquired resistance and fatal tumor metastasis of human colorectal cancer (CRC) are propelling the exploration for novel selective anticarcinogens. Solasodine is a main active component isolated from Solanum incanum L that exhibited a potent stemness and invasion inhibitory effect on human colorectal cancer HCT116 cells. Colony Spheroid formation assay showed that Solasodine dose-dependently prohibited HCT116 cell stemness. CD133, CD44, Nanog, Oct-4 and Sox-2 were inhibited by Solasodine to reverse stemness and similar mechanism was stimulated in vivo. Transwell and scratch wound assays revealed that Solasodine impeded HCT116 cell invasion and migration potential strengthened by TGF-β1. Moreover, Solasodine attenuated TGF-β1-induced EMT and decreased MMPs while in vivo study showed the same trend. The results of this study implied that Solasodine may be a novel therapeutic drug for CRC treatment.
-
Solasodine inhibits human colorectal cancer cells through suppression of the akt glycogen synthase kinase 3β β catenin pathway
Cancer Science, 2017Co-Authors: Yuwen Zhuang, Cunen Wu, Jinyong Zhou, Xu Chen, Jian Wu, Shan Jiang, Haiyan Peng, Dapeng Wu, Tao GongAbstract:Summary Solasodine is a main active component isolated from Solanum incanum L, who performs a wide range of functions containing anti-oxidant, anti-infection and neurogenesis promotion. In this study, we explored the influence of Solasodine on three types of human colorectal cancer (CRC) cell lines. The consequences displayed that Solasodine apparently prohibited CRC cell proliferation dose- and time-dependently and impeded CRC cell motility by downregulating Matrix metalloprotease (MMPs). Besides, Solasodine was investigated to fuel caspase-cascade reaction and increase the ratio between Bax and Bcl-2 so as to induce CRC cell apoptosis. When cells got pretreated with AKT activator (IGF-1) followed by Solasodine application, Solasodine-induced apoptosis was partially confronted by IGF-1. Moreover, Solasodine hindered tumor development and stimulated similar mechanisms in vivo. In general, our study provides the first evidence that Solasodine has a suppressive efficient on CRC cells and that this agent may have potency to be a novel therapeutic drug for CRC treatment. This article is protected by copyright. All rights reserved.
Ana Maria Giulietti - One of the best experts on this subject based on the ideXlab platform.
-
influence of fungal elicitors on Solasodine production by suspension cultures of solanum eleagnifolium
Fitoterapia, 1995Co-Authors: Norma Paniego, J R Talou, Ana Maria GiuliettiAbstract:The effect of fugal elicitors on three cell lines of S. elegnifolium on Solasodine production in batch suspension culture was studied. Autoclaved mycelia of Alternaria sp. and Clamydosporium sp. at 2.5 and 1.0% w/v respectively produced the highest response to elicitation. In fact, Solasodine accumulation increased from 2.5±0.2 mg/g DW to 18.0±2.1 mg/g DW when Alternaria sp. was used as elicitor
-
Solasodine production in transformed organ cultures roots and shoots of solanum eleagnifolium cav
Biotechnology Letters, 1994Co-Authors: M A Alvarez, Rodriguez J Talou, Norma Paniego, Ana Maria GiuliettiAbstract:Transformed organ cultures of Solanum eleagnifolium Cav. (roots and shoots) were obtained by infection with different Agrobacterium species (A. rhizogenes and A. tumefaciens). The growth index and the Solasodine productivity of both kinds of cultures were determinated for several clones. Transformed roots (clone 5) shows a Growth Index of 39.07 and a Solasodine yield of 1.90 ± 0.08 mg g-1DW . Solasodine content in clone 5 of transformed roots (1.9 ± 0.08 mg g-1 DW) is similar to those obtained by hormonal manipulation (1.00 – 1.68 mg g-1DW) but higher than those found in shoots and leaves of the wild-grown plant (0.3–0.4 mg g-1DW).
-
effect of elicitation on the accumulation of Solasodine by immobilized cells of solanum eleagnifolium cav
Enzyme and Microbial Technology, 1993Co-Authors: L E N Quadri, Ana Maria GiuliettiAbstract:Abstract The effect of a fungal elicitor obtained from Alternaria sp. on growth and Solasodine production by free and alginate-entrapped cells of Solanum eleagnifolium Cav. was studied. Fourteen-day-old cultures were elicited with 1% FW/V autoclaved homogenates. The Solasodine production increased from 0.9 to 1.5 mg g -1 DW (65%) in suspension cultures and from 0.75 to 1.4 mg g -1 DW (about 95%) in entrapped cells. The maximum accumulation was obtained after 72 h of elicitation. In order to induce alkaloid release from cells (suspension and entrapped cells), permeabilization with 10% dimethylsulfoxide (DMSO) for 30 min was used. In both cases (free and entrapped cells), about 50–60% of intracellular Solasodine was released into the medium. The reuse of elicited and permeabilized entrapped cells was also carried out for three production cycles.
-
Solasodine production by solanum eleagnifolium cav in vitro cultures influence of plant growth regulators age and inoculum size large scale production
Natural Product Letters, 1993Co-Authors: Maria Alejandra Alvarez, H. M. Nigra, Ana Maria GiuliettiAbstract:Abstract The effect of different auxins (2,4-D, 2,4,5-T, NAA and IAA) on calli maintenance and plant cell suspension initiation as well as the influence of auxins in combination with citoquinines on growth and Solasodine productivity by cell suspensions of Solanum eleagnifolium Cav. was studied. 2,4-D and 2,4,5-T produced a friable and productive calli line and a fine and homogeneous cell suspension cultures. 2,4-D (0.5 μM or 5 μM) or NAA (50 μM) produced the highest growth index and Solasodine productivity with values of about 0.5 mg 1−1 day−1 to 0.8 mg 1−1 day−1. Simultaneous administration of auxin and cytokinin increased the Solasodine productivity with a diminution of cell growth. The combination of NAA (50 μM) with kinetin (0.25 μM) produced an increase of Solasodine productivity from 0.8 mg 1−1 day−1 to 2.54 mg 1−1 day−1. An inoculum size of 5% V/V and 19-day-old was selected to be used in fermentation processes in order to obtain Solasodine production by S. eleagnifolium Cav. suspension cultures. ...
-
effect of carbon and nitrogen sources on growth and Solasodine production in batch suspension cultures of solanum eleagnifolium cav
Plant Cell Tissue and Organ Culture, 1990Co-Authors: H. M. Nigra, Maria Alejandra Alvarez, Ana Maria GiuliettiAbstract:The effect of inoculum size, carbon sources (fructose, glucose, maltose, sucrose), nitrate and ammonia on Solasodine production by Solanum eleagnifolium Cav. was studied. The specific growth rate was estimated to be 0.15–0.20 d-1 with all sugars tested at a concentration of 90 mM. Sucrose (180 mM) produced the highest biomass value (about 2.8 mg DW ml-1) while the lowest one was produced by maltose. Although Solasodine productivity values after 11 days of culture were similar for all sugars tested, the maximum values of productivity (0.9 mg g-1 d-1) were achieved after 6 days of culture with sucrose (180 mM). Solasodine productivity of cultures conducted with a large inoculum (20% w/v fresh material) was double that with a small inoculum (10% w/v fresh material).
John R Porter - One of the best experts on this subject based on the ideXlab platform.
-
an improved high performance liquid chromatographic method for the quantification of Solasodine
Phytochemical Analysis, 1999Co-Authors: Nisit Kittipongpatana, John R Porter, Rick S HockAbstract:An improved isocratic high performance liquid chromatographic method for the quantification of Solasodine, an alternative precursor for the commercial production of steroid drugs, is reported. A highly sensitive and reproducible assay for Solasodine quantification was achieved using a base-deactivated C-8 column and an acidic mobile phase. Sharp symmetrical peaks were obtained and a linear relationship between the amount of Solasodine injected and the area under the peak was achieved. The method allows the simultaneous identification and quantification of Solasodine in plant tissue samples at amounts as low as 0.40 μg, and reports the actual amounts of Solasodine in the sample rather than the “Solasodine equivalents” as determined by previously published methods. Copyright © 1999 John Wiley & Sons, Ltd.
-
Production of Solasodine by hairy root, callus, and cell suspension cultures of Solanum aviculare Forst.
Plant Cell Tissue and Organ Culture, 1998Co-Authors: Nisit Kittipongpatana, Rick S Hock, John R PorterAbstract:The production of the steroidal alkaloid Solasodine, an alternative to diosgenin as a precursor for the commercial production of steroid drugs, was studied in hairy root, callus, and cell suspension cultures of Solanum aviculare Forst. through manipulation of culture medium. The individual and combined effects of medium components on the growth index and the production of Solasodine were analyzed using factorial analysis of variance. Solasodine content was optimized to 6.2 mg g^−1in the hairy root, 1.4 mg g^−1callus, and 0.7 mg g^−1in cell suspension cultures (dry weight). An improved isocratic reversed phase high performance liquid chromatographic method provided selective determination of the Solasodine content of these samples. Analysis of growth and Solasodine content of hairy root cultures and callus cultures demonstrated that the production of Solasodine was shown to be growth-dependent in hairy root cultures but not in callus cultures.
Nisit Kittipongpatana - One of the best experts on this subject based on the ideXlab platform.
-
an improved high performance liquid chromatographic method for the quantification of Solasodine
Phytochemical Analysis, 1999Co-Authors: Nisit Kittipongpatana, John R Porter, Rick S HockAbstract:An improved isocratic high performance liquid chromatographic method for the quantification of Solasodine, an alternative precursor for the commercial production of steroid drugs, is reported. A highly sensitive and reproducible assay for Solasodine quantification was achieved using a base-deactivated C-8 column and an acidic mobile phase. Sharp symmetrical peaks were obtained and a linear relationship between the amount of Solasodine injected and the area under the peak was achieved. The method allows the simultaneous identification and quantification of Solasodine in plant tissue samples at amounts as low as 0.40 μg, and reports the actual amounts of Solasodine in the sample rather than the “Solasodine equivalents” as determined by previously published methods. Copyright © 1999 John Wiley & Sons, Ltd.
-
Production of Solasodine by hairy root, callus, and cell suspension cultures of Solanum aviculare Forst.
Plant Cell Tissue and Organ Culture, 1998Co-Authors: Nisit Kittipongpatana, Rick S Hock, John R PorterAbstract:The production of the steroidal alkaloid Solasodine, an alternative to diosgenin as a precursor for the commercial production of steroid drugs, was studied in hairy root, callus, and cell suspension cultures of Solanum aviculare Forst. through manipulation of culture medium. The individual and combined effects of medium components on the growth index and the production of Solasodine were analyzed using factorial analysis of variance. Solasodine content was optimized to 6.2 mg g^−1in the hairy root, 1.4 mg g^−1callus, and 0.7 mg g^−1in cell suspension cultures (dry weight). An improved isocratic reversed phase high performance liquid chromatographic method provided selective determination of the Solasodine content of these samples. Analysis of growth and Solasodine content of hairy root cultures and callus cultures demonstrated that the production of Solasodine was shown to be growth-dependent in hairy root cultures but not in callus cultures.
Rick S Hock - One of the best experts on this subject based on the ideXlab platform.
-
an improved high performance liquid chromatographic method for the quantification of Solasodine
Phytochemical Analysis, 1999Co-Authors: Nisit Kittipongpatana, John R Porter, Rick S HockAbstract:An improved isocratic high performance liquid chromatographic method for the quantification of Solasodine, an alternative precursor for the commercial production of steroid drugs, is reported. A highly sensitive and reproducible assay for Solasodine quantification was achieved using a base-deactivated C-8 column and an acidic mobile phase. Sharp symmetrical peaks were obtained and a linear relationship between the amount of Solasodine injected and the area under the peak was achieved. The method allows the simultaneous identification and quantification of Solasodine in plant tissue samples at amounts as low as 0.40 μg, and reports the actual amounts of Solasodine in the sample rather than the “Solasodine equivalents” as determined by previously published methods. Copyright © 1999 John Wiley & Sons, Ltd.
-
Production of Solasodine by hairy root, callus, and cell suspension cultures of Solanum aviculare Forst.
Plant Cell Tissue and Organ Culture, 1998Co-Authors: Nisit Kittipongpatana, Rick S Hock, John R PorterAbstract:The production of the steroidal alkaloid Solasodine, an alternative to diosgenin as a precursor for the commercial production of steroid drugs, was studied in hairy root, callus, and cell suspension cultures of Solanum aviculare Forst. through manipulation of culture medium. The individual and combined effects of medium components on the growth index and the production of Solasodine were analyzed using factorial analysis of variance. Solasodine content was optimized to 6.2 mg g^−1in the hairy root, 1.4 mg g^−1callus, and 0.7 mg g^−1in cell suspension cultures (dry weight). An improved isocratic reversed phase high performance liquid chromatographic method provided selective determination of the Solasodine content of these samples. Analysis of growth and Solasodine content of hairy root cultures and callus cultures demonstrated that the production of Solasodine was shown to be growth-dependent in hairy root cultures but not in callus cultures.
