The Experts below are selected from a list of 3483 Experts worldwide ranked by ideXlab platform
Massimo Morbidelli - One of the best experts on this subject based on the ideXlab platform.
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experimental verification of sample solvent induced modifier Solute Peak interactions in biochromatography
Journal of Chromatography A, 2006Co-Authors: Guido Strohlein, Marco Mazzotti, Lars Aumann, Lena Melter, Klaus Buscher, Berthold Schenkel, Massimo MorbidelliAbstract:In a previous theoretical analysis based on equilibrium theory it has been shown how differences in the sample and elution modifier concentrations can lead to unexpected behavior of the Solute eluted Peaks such as retention time distortion, Peak deformation and Peak doubling. All these features are verified experimentally in this work using the polypeptide calcitonin and a variant of a specific monoclonal antibody as chromatographic model systems. For both experimental systems, the retention time distortion can be predicted with high accuracy by the solution of the equilibrium theory model. For the polypeptide, the predictions from the theory about the occurrence of Peak deformation and double Peaks has been successfully verified by a series of tailored experiments with positive as well as negative modifier perturbations.
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modeling of modifier Solute Peak interactions in chromatography
Aiche Journal, 2006Co-Authors: Guido Strohlein, Massimo Morbidelli, Hyunku Rhee, Marco MazzottiAbstract:The behavior of a chromatographic column where a single Solute is fed in the presence of a suitable modifier is analyzed in the context of equilibrium theory. In particular, the pulse propagation of the Solute, whose retention depends on the modifier concentration, has been analyzed as a full-fledged binary problem by the method of hodograph transformation applied to the pair of quasilinear differential equations. It has been shown that all possible operating conditions can be summarized with respect to the modifier concentration into three cases. These have been solved analytically by constructing the solution first in the hodograph plane, and then mapping it onto the physical plane using the method of characteristics. The peculiar properties of the derived hodograph plane were explained, and their impact on the pulse propagation for the three different cases has been elucidated. For the cases of intermediate adsorptivity of the modifier, the occurrence of Solute Peaks with infinitely high concentrations; that is, Solute impulse, has been proven as well as the phenomena of double Solute Peaks.
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analysis of sample solvent induced modifier Solute Peak interactions in biochromatography using equilibrium theory and detailed simulations
Journal of Chromatography A, 2005Co-Authors: Guido Strohlein, Marco Mazzotti, Massimo MorbidelliAbstract:Batch liquid chromatographic columns are often equilibrated with an eluent stream being a mixture of inert compounds and so-called modifiers. The sample injected into the eluent stream usually consists of the Solutes to be separated and of a mixture of the same solvents as in the eluent but in general with different concentration values. This results in two groups of Peaks moving along the column: the Solute Peaks and the modifier pertubations. If the adsorptivity of the Solute depends strongly on the modifier, as it is often the case in biochromatography, the interference between the two groups of Peaks leads to peculiar phenomena like double Peaks, split Peaks, distorted Peaks with anti-Langmurian shape, etc. In this work, these phenomena are analyzed based on an analytical solution of the equilibrium theory model and the results are compared with detailed simulations and experimental data. It is shown that the qualitative behavior is well predicted in the frame of equilibrium theory and general guidelines how to avoid these kinds of interactions are developed.
Guido Strohlein - One of the best experts on this subject based on the ideXlab platform.
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experimental verification of sample solvent induced modifier Solute Peak interactions in biochromatography
Journal of Chromatography A, 2006Co-Authors: Guido Strohlein, Marco Mazzotti, Lars Aumann, Lena Melter, Klaus Buscher, Berthold Schenkel, Massimo MorbidelliAbstract:In a previous theoretical analysis based on equilibrium theory it has been shown how differences in the sample and elution modifier concentrations can lead to unexpected behavior of the Solute eluted Peaks such as retention time distortion, Peak deformation and Peak doubling. All these features are verified experimentally in this work using the polypeptide calcitonin and a variant of a specific monoclonal antibody as chromatographic model systems. For both experimental systems, the retention time distortion can be predicted with high accuracy by the solution of the equilibrium theory model. For the polypeptide, the predictions from the theory about the occurrence of Peak deformation and double Peaks has been successfully verified by a series of tailored experiments with positive as well as negative modifier perturbations.
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modeling of modifier Solute Peak interactions in chromatography
Aiche Journal, 2006Co-Authors: Guido Strohlein, Massimo Morbidelli, Hyunku Rhee, Marco MazzottiAbstract:The behavior of a chromatographic column where a single Solute is fed in the presence of a suitable modifier is analyzed in the context of equilibrium theory. In particular, the pulse propagation of the Solute, whose retention depends on the modifier concentration, has been analyzed as a full-fledged binary problem by the method of hodograph transformation applied to the pair of quasilinear differential equations. It has been shown that all possible operating conditions can be summarized with respect to the modifier concentration into three cases. These have been solved analytically by constructing the solution first in the hodograph plane, and then mapping it onto the physical plane using the method of characteristics. The peculiar properties of the derived hodograph plane were explained, and their impact on the pulse propagation for the three different cases has been elucidated. For the cases of intermediate adsorptivity of the modifier, the occurrence of Solute Peaks with infinitely high concentrations; that is, Solute impulse, has been proven as well as the phenomena of double Solute Peaks.
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analysis of sample solvent induced modifier Solute Peak interactions in biochromatography using equilibrium theory and detailed simulations
Journal of Chromatography A, 2005Co-Authors: Guido Strohlein, Marco Mazzotti, Massimo MorbidelliAbstract:Batch liquid chromatographic columns are often equilibrated with an eluent stream being a mixture of inert compounds and so-called modifiers. The sample injected into the eluent stream usually consists of the Solutes to be separated and of a mixture of the same solvents as in the eluent but in general with different concentration values. This results in two groups of Peaks moving along the column: the Solute Peaks and the modifier pertubations. If the adsorptivity of the Solute depends strongly on the modifier, as it is often the case in biochromatography, the interference between the two groups of Peaks leads to peculiar phenomena like double Peaks, split Peaks, distorted Peaks with anti-Langmurian shape, etc. In this work, these phenomena are analyzed based on an analytical solution of the equilibrium theory model and the results are compared with detailed simulations and experimental data. It is shown that the qualitative behavior is well predicted in the frame of equilibrium theory and general guidelines how to avoid these kinds of interactions are developed.
Marco Mazzotti - One of the best experts on this subject based on the ideXlab platform.
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experimental verification of sample solvent induced modifier Solute Peak interactions in biochromatography
Journal of Chromatography A, 2006Co-Authors: Guido Strohlein, Marco Mazzotti, Lars Aumann, Lena Melter, Klaus Buscher, Berthold Schenkel, Massimo MorbidelliAbstract:In a previous theoretical analysis based on equilibrium theory it has been shown how differences in the sample and elution modifier concentrations can lead to unexpected behavior of the Solute eluted Peaks such as retention time distortion, Peak deformation and Peak doubling. All these features are verified experimentally in this work using the polypeptide calcitonin and a variant of a specific monoclonal antibody as chromatographic model systems. For both experimental systems, the retention time distortion can be predicted with high accuracy by the solution of the equilibrium theory model. For the polypeptide, the predictions from the theory about the occurrence of Peak deformation and double Peaks has been successfully verified by a series of tailored experiments with positive as well as negative modifier perturbations.
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modeling of modifier Solute Peak interactions in chromatography
Aiche Journal, 2006Co-Authors: Guido Strohlein, Massimo Morbidelli, Hyunku Rhee, Marco MazzottiAbstract:The behavior of a chromatographic column where a single Solute is fed in the presence of a suitable modifier is analyzed in the context of equilibrium theory. In particular, the pulse propagation of the Solute, whose retention depends on the modifier concentration, has been analyzed as a full-fledged binary problem by the method of hodograph transformation applied to the pair of quasilinear differential equations. It has been shown that all possible operating conditions can be summarized with respect to the modifier concentration into three cases. These have been solved analytically by constructing the solution first in the hodograph plane, and then mapping it onto the physical plane using the method of characteristics. The peculiar properties of the derived hodograph plane were explained, and their impact on the pulse propagation for the three different cases has been elucidated. For the cases of intermediate adsorptivity of the modifier, the occurrence of Solute Peaks with infinitely high concentrations; that is, Solute impulse, has been proven as well as the phenomena of double Solute Peaks.
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analysis of sample solvent induced modifier Solute Peak interactions in biochromatography using equilibrium theory and detailed simulations
Journal of Chromatography A, 2005Co-Authors: Guido Strohlein, Marco Mazzotti, Massimo MorbidelliAbstract:Batch liquid chromatographic columns are often equilibrated with an eluent stream being a mixture of inert compounds and so-called modifiers. The sample injected into the eluent stream usually consists of the Solutes to be separated and of a mixture of the same solvents as in the eluent but in general with different concentration values. This results in two groups of Peaks moving along the column: the Solute Peaks and the modifier pertubations. If the adsorptivity of the Solute depends strongly on the modifier, as it is often the case in biochromatography, the interference between the two groups of Peaks leads to peculiar phenomena like double Peaks, split Peaks, distorted Peaks with anti-Langmurian shape, etc. In this work, these phenomena are analyzed based on an analytical solution of the equilibrium theory model and the results are compared with detailed simulations and experimental data. It is shown that the qualitative behavior is well predicted in the frame of equilibrium theory and general guidelines how to avoid these kinds of interactions are developed.
Berthold Schenkel - One of the best experts on this subject based on the ideXlab platform.
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experimental verification of sample solvent induced modifier Solute Peak interactions in biochromatography
Journal of Chromatography A, 2006Co-Authors: Guido Strohlein, Marco Mazzotti, Lars Aumann, Lena Melter, Klaus Buscher, Berthold Schenkel, Massimo MorbidelliAbstract:In a previous theoretical analysis based on equilibrium theory it has been shown how differences in the sample and elution modifier concentrations can lead to unexpected behavior of the Solute eluted Peaks such as retention time distortion, Peak deformation and Peak doubling. All these features are verified experimentally in this work using the polypeptide calcitonin and a variant of a specific monoclonal antibody as chromatographic model systems. For both experimental systems, the retention time distortion can be predicted with high accuracy by the solution of the equilibrium theory model. For the polypeptide, the predictions from the theory about the occurrence of Peak deformation and double Peaks has been successfully verified by a series of tailored experiments with positive as well as negative modifier perturbations.
Klaus Buscher - One of the best experts on this subject based on the ideXlab platform.
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experimental verification of sample solvent induced modifier Solute Peak interactions in biochromatography
Journal of Chromatography A, 2006Co-Authors: Guido Strohlein, Marco Mazzotti, Lars Aumann, Lena Melter, Klaus Buscher, Berthold Schenkel, Massimo MorbidelliAbstract:In a previous theoretical analysis based on equilibrium theory it has been shown how differences in the sample and elution modifier concentrations can lead to unexpected behavior of the Solute eluted Peaks such as retention time distortion, Peak deformation and Peak doubling. All these features are verified experimentally in this work using the polypeptide calcitonin and a variant of a specific monoclonal antibody as chromatographic model systems. For both experimental systems, the retention time distortion can be predicted with high accuracy by the solution of the equilibrium theory model. For the polypeptide, the predictions from the theory about the occurrence of Peak deformation and double Peaks has been successfully verified by a series of tailored experiments with positive as well as negative modifier perturbations.