The Experts below are selected from a list of 24 Experts worldwide ranked by ideXlab platform

Sadakazu Aiso - One of the best experts on this subject based on the ideXlab platform.

  • a composite hormone response element regulates transcription of the rat ghrh receptor gene
    Endocrinology, 2002
    Co-Authors: Haruo Nogami, Yoshiki Hiraoka, Maki Matsubara, Eriko Nonobe, Toshio Harigaya, Masateru Katayama, Noriaki Hemmi, Shuzo Kobayashi, Koichi Mogi, Sadakazu Aiso
    Abstract:

    To further elucidate the molecular mechanisms underlying the transcriptional regulation of the GHRH receptor (GHRH-R) gene, hormonal regulation of the promoter activity of this gene was examined. An approximately 3-kb genomic fragment spanning the promoter region of the gene was sequenced and the transcription start site was determined by RT-PCR and RNase protection assay. A major start site was localized at −105 (relative to the translation initiation codon, ATG), and a pit-1 binding sequence characteristic of pituitary specific genes was found at −155 to −146. Deletion and mutation studies demonstrated this site to be functional. In the presence of dexamethasone, the GHRH-R promoter (from −2935 to −11) directed luciferase expression in MtT-S Cells, a Somatotropic Cell line, but not in the PC12 Cells that normally do not express GHRH-R. While T3, all trans-RA, and 9cis-RA alone weakly enhanced the reporter gene expression, each of these substances was found to act as a synergistic enhancer in the presenc...

Bello A. R. - One of the best experts on this subject based on the ideXlab platform.

  • Origin of adenohypophysial lobes and Cells from Rathke's pouch in Swiss albino mice. Proliferation and expression of Pitx 2 and calbindin D28K in corticotropic and Somatotropic Cell differentiation
    'Wiley', 2012
    Co-Authors: Reyes R., Martínez S., González Miriam, Tramu G., Bello A. R.
    Abstract:

    A developmental study of the adenohypophysis of the mouse was carried out in response to several as yet unanswered questions about its organogenesis and differentiation. The main objectives were to establish the origin of adenohypophysial lobes and Cells from the Rathke's pouch (RP) and elucidate the mechanisms of development and functional differentiation of the gland. Using diverse techniques, the morphological development, proliferation and differentiation were studied in order to delimit different proliferative regions in the RP, and provide a satisfactory explanation for the distribution of each Cell type in the adult gland. Combining the proliferation and differentiation studies, corticotropic and Somatotropic Cells appear to mainly originate from undifferentiated precursors located within each of these proliferative regions. The involvement of transcription factor Pitx 2 and calcium-binding protein Calbindin D 28K in the differentiation of corticotropic and Somatotropic Cells is further clarified. © 2008 The Authors.This work was supported by DGYCIT PB97-1472-CO3-02/01(A.R.B. and R.A) and by BIOMED2: BMH4 CT96 0249 projects and by European Union grants QLRT-1999-31556, 1999-31625, 2000-02310 and grant DGI BFI2002-02979 (to SM).Peer Reviewe

Haruo Nogami - One of the best experts on this subject based on the ideXlab platform.

  • a composite hormone response element regulates transcription of the rat ghrh receptor gene
    Endocrinology, 2002
    Co-Authors: Haruo Nogami, Yoshiki Hiraoka, Maki Matsubara, Eriko Nonobe, Toshio Harigaya, Masateru Katayama, Noriaki Hemmi, Shuzo Kobayashi, Koichi Mogi, Sadakazu Aiso
    Abstract:

    To further elucidate the molecular mechanisms underlying the transcriptional regulation of the GHRH receptor (GHRH-R) gene, hormonal regulation of the promoter activity of this gene was examined. An approximately 3-kb genomic fragment spanning the promoter region of the gene was sequenced and the transcription start site was determined by RT-PCR and RNase protection assay. A major start site was localized at −105 (relative to the translation initiation codon, ATG), and a pit-1 binding sequence characteristic of pituitary specific genes was found at −155 to −146. Deletion and mutation studies demonstrated this site to be functional. In the presence of dexamethasone, the GHRH-R promoter (from −2935 to −11) directed luciferase expression in MtT-S Cells, a Somatotropic Cell line, but not in the PC12 Cells that normally do not express GHRH-R. While T3, all trans-RA, and 9cis-RA alone weakly enhanced the reporter gene expression, each of these substances was found to act as a synergistic enhancer in the presenc...

Reyes R. - One of the best experts on this subject based on the ideXlab platform.

  • Origin of adenohypophysial lobes and Cells from Rathke's pouch in Swiss albino mice. Proliferation and expression of Pitx 2 and calbindin D28K in corticotropic and Somatotropic Cell differentiation
    'Wiley', 2012
    Co-Authors: Reyes R., Martínez S., González Miriam, Tramu G., Bello A. R.
    Abstract:

    A developmental study of the adenohypophysis of the mouse was carried out in response to several as yet unanswered questions about its organogenesis and differentiation. The main objectives were to establish the origin of adenohypophysial lobes and Cells from the Rathke's pouch (RP) and elucidate the mechanisms of development and functional differentiation of the gland. Using diverse techniques, the morphological development, proliferation and differentiation were studied in order to delimit different proliferative regions in the RP, and provide a satisfactory explanation for the distribution of each Cell type in the adult gland. Combining the proliferation and differentiation studies, corticotropic and Somatotropic Cells appear to mainly originate from undifferentiated precursors located within each of these proliferative regions. The involvement of transcription factor Pitx 2 and calcium-binding protein Calbindin D 28K in the differentiation of corticotropic and Somatotropic Cells is further clarified. © 2008 The Authors.This work was supported by DGYCIT PB97-1472-CO3-02/01(A.R.B. and R.A) and by BIOMED2: BMH4 CT96 0249 projects and by European Union grants QLRT-1999-31556, 1999-31625, 2000-02310 and grant DGI BFI2002-02979 (to SM).Peer Reviewe

Aixa R Bello - One of the best experts on this subject based on the ideXlab platform.