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Franzrainer Matuschka - One of the best experts on this subject based on the ideXlab platform.
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absence of lyme disease spirochetes in larval ixodes ricinus ticks
Vector-borne and Zoonotic Diseases, 2012Co-Authors: Dania Richter, Alina Debski, Zdenek Hubalek, Franzrainer MatuschkaAbstract:Abstract To determine which kind of spirochete infects larval Ixodes ricinus, we examined questing larvae and larvae derived from engorged females for the presence of particular spirochetal DNA that permitted Species Differentiation. Borrelia miyamotoi was the sole spirochete detected in larval ticks sampled while questing on vegetation. Questing nymphal and adult ticks were infected mainly by Borrelia afzelii, whereas larval ticks resulting from engorged females of the same population were solely infected by B. miyamotoi. Since larvae acquire Lyme disease spirochetes within a few hours of attachment to an infected rodent, questing larvae in nature may have acquired Lyme disease spirochetes from an interrupted host contact. Even if transovarial transmission of Lyme disease spirochetes may occasionally occur, it seems to be an exceedingly rare event. No undisputable proof exists for vertical transmission of Lyme disease spirochetes, whereas B. miyamotoi appears to be readily passed between generations of v...
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Absence of Lyme disease spirochetes in larval Ixodes ricinus ticks.
Vector borne and zoonotic diseases (Larchmont N.Y.), 2011Co-Authors: Dania Richter, Alina Debski, Zdenek Hubalek, Franzrainer MatuschkaAbstract:Abstract To determine which kind of spirochete infects larval Ixodes ricinus, we examined questing larvae and larvae derived from engorged females for the presence of particular spirochetal DNA that permitted Species Differentiation. Borrelia miyamotoi was the sole spirochete detected in larval ticks sampled while questing on vegetation. Questing nymphal and adult ticks were infected mainly by Borrelia afzelii, whereas larval ticks resulting from engorged females of the same population were solely infected by B. miyamotoi. Since larvae acquire Lyme disease spirochetes within a few hours of attachment to an infected rodent, questing larvae in nature may have acquired Lyme disease spirochetes from an interrupted host contact. Even if transovarial transmission of Lyme disease spirochetes may occasionally occur, it seems to be an exceedingly rare event. No undisputable proof exists for vertical transmission of Lyme disease spirochetes, whereas B. miyamotoi appears to be readily passed between generations of v...
Theresa Zwickel - One of the best experts on this subject based on the ideXlab platform.
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chemotaxonomy of mycotoxigenic small spored alternaria fungi do multitoxin mixtures act as an indicator for Species Differentiation
Frontiers in Microbiology, 2018Co-Authors: Michael Rychlik, Theresa Zwickel, Sandra M. Kahl, Marina E. H. MüllerAbstract:Necrotrophic as well as saprophytic small-spored Alternaria (A.) Species are annually responsible for major losses of agricultural products, such as cereal crops, associated with the contamination of food and feedstuff with potential health-endangering Alternaria toxins. Knowledge of the metabolic capabilities of different Species-groups to form mycotoxins is of importance for a reliable risk assessment. 93 Alternaria strains belonging to the four Species groups Alternaria tenuissima, A. arborescens, A. alternata, and A. infectoria were isolated from winter wheat kernels harvested from fields in Germany and Russia and incubated under equal conditions. Chemical analysis by means of an HPLC-MS/MS multi-Alternaria-toxin-method showed that 95% of all strains were able to form at least one of the targeted 17 non-host specific Alternaria toxins. Simultaneous production of up to 15 (modified) Alternaria toxins by members of the A. tenuissima, A. arborescens, A. alternata Species-groups and up to seven toxins by A. infectoria strains was demonstrated. Overall tenuazonic acid was the most extensively formed mycotoxin followed by alternariol and alternariol mono methylether, whereas altertoxin I was the most frequently detected toxin. Sulfoconjugated modifications of alternariol, alternariol mono methylether, altenuisol and altenuene were frequently determined. Unknown perylene quinone derivatives were additionally detected. Strains of the Species-group A. infectoria could be segregated from strains of the other three Species-groups due to significantly lower toxin levels and the specific production of infectopyrone. Apart from infectopyrone, alterperylenol was also frequently produced by 95% of the A. infectoria strains. Neither by the concentration nor by the composition of the targeted Alternaria toxins a Differentiation between the Species-groups A. alternata, A. tenuissima and A. arborescens was possible.
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Table_1_Chemotaxonomy of Mycotoxigenic Small-Spored Alternaria Fungi – Do Multitoxin Mixtures Act as an Indicator for Species Differentiation?.pdf
2018Co-Authors: Theresa Zwickel, Michael Rychlik, Sandra M. Kahl, Marina E. H. MüllerAbstract:Necrotrophic as well as saprophytic small-spored Alternaria (A.) Species are annually responsible for major losses of agricultural products, such as cereal crops, associated with the contamination of food and feedstuff with potential health-endangering Alternaria toxins. Knowledge of the metabolic capabilities of different Species-groups to form mycotoxins is of importance for a reliable risk assessment. 93 Alternaria strains belonging to the four Species groups Alternaria tenuissima, A. arborescens, A. alternata, and A. infectoria were isolated from winter wheat kernels harvested from fields in Germany and Russia and incubated under equal conditions. Chemical analysis by means of an HPLC-MS/MS multi-Alternaria-toxin-method showed that 95% of all strains were able to form at least one of the targeted 17 non-host specific Alternaria toxins. Simultaneous production of up to 15 (modified) Alternaria toxins by members of the A. tenuissima, A. arborescens, A. alternata Species-groups and up to seven toxins by A. infectoria strains was demonstrated. Overall tenuazonic acid was the most extensively formed mycotoxin followed by alternariol and alternariol mono methylether, whereas altertoxin I was the most frequently detected toxin. Sulfoconjugated modifications of alternariol, alternariol mono methylether, altenuisol and altenuene were frequently determined. Unknown perylene quinone derivatives were additionally detected. Strains of the Species-group A. infectoria could be segregated from strains of the other three Species-groups due to significantly lower toxin levels and the specific production of infectopyrone. Apart from infectopyrone, alterperylenol was also frequently produced by 95% of the A. infectoria strains. Neither by the concentration nor by the composition of the targeted Alternaria toxins a Differentiation between the Species-groups A. alternata, A. tenuissima and A. arborescens was possible.
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Chemotaxonomy of Mycotoxigenic Small-Spored Alternaria Fungi – Do Multitoxin Mixtures Act as an Indicator for Species Differentiation?
Frontiers Media S.A., 2018Co-Authors: Michael Rychlik, Theresa Zwickel, Sandra M. Kahl, Marina E. H. MüllerAbstract:Necrotrophic as well as saprophytic small-spored Alternaria (A.) Species are annually responsible for major losses of agricultural products, such as cereal crops, associated with the contamination of food and feedstuff with potential health-endangering Alternaria toxins. Knowledge of the metabolic capabilities of different Species-groups to form mycotoxins is of importance for a reliable risk assessment. 93 Alternaria strains belonging to the four Species groups Alternaria tenuissima, A. arborescens, A. alternata, and A. infectoria were isolated from winter wheat kernels harvested from fields in Germany and Russia and incubated under equal conditions. Chemical analysis by means of an HPLC-MS/MS multi-Alternaria-toxin-method showed that 95% of all strains were able to form at least one of the targeted 17 non-host specific Alternaria toxins. Simultaneous production of up to 15 (modified) Alternaria toxins by members of the A. tenuissima, A. arborescens, A. alternata Species-groups and up to seven toxins by A. infectoria strains was demonstrated. Overall tenuazonic acid was the most extensively formed mycotoxin followed by alternariol and alternariol mono methylether, whereas altertoxin I was the most frequently detected toxin. Sulfoconjugated modifications of alternariol, alternariol mono methylether, altenuisol and altenuene were frequently determined. Unknown perylene quinone derivatives were additionally detected. Strains of the Species-group A. infectoria could be segregated from strains of the other three Species-groups due to significantly lower toxin levels and the specific production of infectopyrone. Apart from infectopyrone, alterperylenol was also frequently produced by 95% of the A. infectoria strains. Neither by the concentration nor by the composition of the targeted Alternaria toxins a Differentiation between the Species-groups A. alternata, A. tenuissima and A. arborescens was possible
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Image_2_Chemotaxonomy of Mycotoxigenic Small-Spored Alternaria Fungi – Do Multitoxin Mixtures Act as an Indicator for Species Differentiation?.pdf
2018Co-Authors: Theresa Zwickel, Michael Rychlik, Sandra M. Kahl, Marina E. H. MüllerAbstract:Necrotrophic as well as saprophytic small-spored Alternaria (A.) Species are annually responsible for major losses of agricultural products, such as cereal crops, associated with the contamination of food and feedstuff with potential health-endangering Alternaria toxins. Knowledge of the metabolic capabilities of different Species-groups to form mycotoxins is of importance for a reliable risk assessment. 93 Alternaria strains belonging to the four Species groups Alternaria tenuissima, A. arborescens, A. alternata, and A. infectoria were isolated from winter wheat kernels harvested from fields in Germany and Russia and incubated under equal conditions. Chemical analysis by means of an HPLC-MS/MS multi-Alternaria-toxin-method showed that 95% of all strains were able to form at least one of the targeted 17 non-host specific Alternaria toxins. Simultaneous production of up to 15 (modified) Alternaria toxins by members of the A. tenuissima, A. arborescens, A. alternata Species-groups and up to seven toxins by A. infectoria strains was demonstrated. Overall tenuazonic acid was the most extensively formed mycotoxin followed by alternariol and alternariol mono methylether, whereas altertoxin I was the most frequently detected toxin. Sulfoconjugated modifications of alternariol, alternariol mono methylether, altenuisol and altenuene were frequently determined. Unknown perylene quinone derivatives were additionally detected. Strains of the Species-group A. infectoria could be segregated from strains of the other three Species-groups due to significantly lower toxin levels and the specific production of infectopyrone. Apart from infectopyrone, alterperylenol was also frequently produced by 95% of the A. infectoria strains. Neither by the concentration nor by the composition of the targeted Alternaria toxins a Differentiation between the Species-groups A. alternata, A. tenuissima and A. arborescens was possible.
Marina E. H. Müller - One of the best experts on this subject based on the ideXlab platform.
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chemotaxonomy of mycotoxigenic small spored alternaria fungi do multitoxin mixtures act as an indicator for Species Differentiation
Frontiers in Microbiology, 2018Co-Authors: Michael Rychlik, Theresa Zwickel, Sandra M. Kahl, Marina E. H. MüllerAbstract:Necrotrophic as well as saprophytic small-spored Alternaria (A.) Species are annually responsible for major losses of agricultural products, such as cereal crops, associated with the contamination of food and feedstuff with potential health-endangering Alternaria toxins. Knowledge of the metabolic capabilities of different Species-groups to form mycotoxins is of importance for a reliable risk assessment. 93 Alternaria strains belonging to the four Species groups Alternaria tenuissima, A. arborescens, A. alternata, and A. infectoria were isolated from winter wheat kernels harvested from fields in Germany and Russia and incubated under equal conditions. Chemical analysis by means of an HPLC-MS/MS multi-Alternaria-toxin-method showed that 95% of all strains were able to form at least one of the targeted 17 non-host specific Alternaria toxins. Simultaneous production of up to 15 (modified) Alternaria toxins by members of the A. tenuissima, A. arborescens, A. alternata Species-groups and up to seven toxins by A. infectoria strains was demonstrated. Overall tenuazonic acid was the most extensively formed mycotoxin followed by alternariol and alternariol mono methylether, whereas altertoxin I was the most frequently detected toxin. Sulfoconjugated modifications of alternariol, alternariol mono methylether, altenuisol and altenuene were frequently determined. Unknown perylene quinone derivatives were additionally detected. Strains of the Species-group A. infectoria could be segregated from strains of the other three Species-groups due to significantly lower toxin levels and the specific production of infectopyrone. Apart from infectopyrone, alterperylenol was also frequently produced by 95% of the A. infectoria strains. Neither by the concentration nor by the composition of the targeted Alternaria toxins a Differentiation between the Species-groups A. alternata, A. tenuissima and A. arborescens was possible.
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Table_1_Chemotaxonomy of Mycotoxigenic Small-Spored Alternaria Fungi – Do Multitoxin Mixtures Act as an Indicator for Species Differentiation?.pdf
2018Co-Authors: Theresa Zwickel, Michael Rychlik, Sandra M. Kahl, Marina E. H. MüllerAbstract:Necrotrophic as well as saprophytic small-spored Alternaria (A.) Species are annually responsible for major losses of agricultural products, such as cereal crops, associated with the contamination of food and feedstuff with potential health-endangering Alternaria toxins. Knowledge of the metabolic capabilities of different Species-groups to form mycotoxins is of importance for a reliable risk assessment. 93 Alternaria strains belonging to the four Species groups Alternaria tenuissima, A. arborescens, A. alternata, and A. infectoria were isolated from winter wheat kernels harvested from fields in Germany and Russia and incubated under equal conditions. Chemical analysis by means of an HPLC-MS/MS multi-Alternaria-toxin-method showed that 95% of all strains were able to form at least one of the targeted 17 non-host specific Alternaria toxins. Simultaneous production of up to 15 (modified) Alternaria toxins by members of the A. tenuissima, A. arborescens, A. alternata Species-groups and up to seven toxins by A. infectoria strains was demonstrated. Overall tenuazonic acid was the most extensively formed mycotoxin followed by alternariol and alternariol mono methylether, whereas altertoxin I was the most frequently detected toxin. Sulfoconjugated modifications of alternariol, alternariol mono methylether, altenuisol and altenuene were frequently determined. Unknown perylene quinone derivatives were additionally detected. Strains of the Species-group A. infectoria could be segregated from strains of the other three Species-groups due to significantly lower toxin levels and the specific production of infectopyrone. Apart from infectopyrone, alterperylenol was also frequently produced by 95% of the A. infectoria strains. Neither by the concentration nor by the composition of the targeted Alternaria toxins a Differentiation between the Species-groups A. alternata, A. tenuissima and A. arborescens was possible.
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Chemotaxonomy of Mycotoxigenic Small-Spored Alternaria Fungi – Do Multitoxin Mixtures Act as an Indicator for Species Differentiation?
Frontiers Media S.A., 2018Co-Authors: Michael Rychlik, Theresa Zwickel, Sandra M. Kahl, Marina E. H. MüllerAbstract:Necrotrophic as well as saprophytic small-spored Alternaria (A.) Species are annually responsible for major losses of agricultural products, such as cereal crops, associated with the contamination of food and feedstuff with potential health-endangering Alternaria toxins. Knowledge of the metabolic capabilities of different Species-groups to form mycotoxins is of importance for a reliable risk assessment. 93 Alternaria strains belonging to the four Species groups Alternaria tenuissima, A. arborescens, A. alternata, and A. infectoria were isolated from winter wheat kernels harvested from fields in Germany and Russia and incubated under equal conditions. Chemical analysis by means of an HPLC-MS/MS multi-Alternaria-toxin-method showed that 95% of all strains were able to form at least one of the targeted 17 non-host specific Alternaria toxins. Simultaneous production of up to 15 (modified) Alternaria toxins by members of the A. tenuissima, A. arborescens, A. alternata Species-groups and up to seven toxins by A. infectoria strains was demonstrated. Overall tenuazonic acid was the most extensively formed mycotoxin followed by alternariol and alternariol mono methylether, whereas altertoxin I was the most frequently detected toxin. Sulfoconjugated modifications of alternariol, alternariol mono methylether, altenuisol and altenuene were frequently determined. Unknown perylene quinone derivatives were additionally detected. Strains of the Species-group A. infectoria could be segregated from strains of the other three Species-groups due to significantly lower toxin levels and the specific production of infectopyrone. Apart from infectopyrone, alterperylenol was also frequently produced by 95% of the A. infectoria strains. Neither by the concentration nor by the composition of the targeted Alternaria toxins a Differentiation between the Species-groups A. alternata, A. tenuissima and A. arborescens was possible
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Image_2_Chemotaxonomy of Mycotoxigenic Small-Spored Alternaria Fungi – Do Multitoxin Mixtures Act as an Indicator for Species Differentiation?.pdf
2018Co-Authors: Theresa Zwickel, Michael Rychlik, Sandra M. Kahl, Marina E. H. MüllerAbstract:Necrotrophic as well as saprophytic small-spored Alternaria (A.) Species are annually responsible for major losses of agricultural products, such as cereal crops, associated with the contamination of food and feedstuff with potential health-endangering Alternaria toxins. Knowledge of the metabolic capabilities of different Species-groups to form mycotoxins is of importance for a reliable risk assessment. 93 Alternaria strains belonging to the four Species groups Alternaria tenuissima, A. arborescens, A. alternata, and A. infectoria were isolated from winter wheat kernels harvested from fields in Germany and Russia and incubated under equal conditions. Chemical analysis by means of an HPLC-MS/MS multi-Alternaria-toxin-method showed that 95% of all strains were able to form at least one of the targeted 17 non-host specific Alternaria toxins. Simultaneous production of up to 15 (modified) Alternaria toxins by members of the A. tenuissima, A. arborescens, A. alternata Species-groups and up to seven toxins by A. infectoria strains was demonstrated. Overall tenuazonic acid was the most extensively formed mycotoxin followed by alternariol and alternariol mono methylether, whereas altertoxin I was the most frequently detected toxin. Sulfoconjugated modifications of alternariol, alternariol mono methylether, altenuisol and altenuene were frequently determined. Unknown perylene quinone derivatives were additionally detected. Strains of the Species-group A. infectoria could be segregated from strains of the other three Species-groups due to significantly lower toxin levels and the specific production of infectopyrone. Apart from infectopyrone, alterperylenol was also frequently produced by 95% of the A. infectoria strains. Neither by the concentration nor by the composition of the targeted Alternaria toxins a Differentiation between the Species-groups A. alternata, A. tenuissima and A. arborescens was possible.
Benito Cañas - One of the best experts on this subject based on the ideXlab platform.
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comparative analysis of protein extraction methods for the identification of seafood borne pathogenic and spoilage bacteria by maldi tof mass spectrometry
Analytical Methods, 2010Co-Authors: Karola Böhme, Inmaculada C Fernandezno, Jorge Barrosvelazquez, Benito Cañas, José Manuel Gallardo, Pilar CalomataAbstract:Species Differentiation of food pathogenic and spoilage bacteria is important to ensure food quality and safety. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been applied to Species identification of microorganisms, proving to be a rapid and cost-effective technique and allowing Species Differentiation due to the highly specific spectral profiles obtained. In this work, bacterial strains from our laboratory intern collection of seafood-borne pathogenic and spoilage Species were studied by MALDI-TOF MS. Different sample preparation protocols were applied and compared to each other. Two methods were based on the analysis of whole bacterial cells that were suspended in an organic solvent or applied directly to the sample target. In a different sample preparation technique, cell extracts were obtained from intact bacterial cells by a dissolution/centrifugation step. The protocol applied for the study of cell extracts was shown to be very fast and simple, allowing the standardization of sample preparation. Furthermore, the analysis of cell extracts had several advantages with respect to the analysis of suspensions of whole bacterial cells. Thus, spectral profiles obtained from cell extracts showed less noise and more reproducible peaks as compared to spectra obtained from intact cells. The analysis of cell extracts by MALDI-TOF MS was also applied to create a mass spectral library of the main pathogenic and spoilage bacteria potentially present in seafood and was demonstrated to be a rapid and accurate method for microbial Species Differentiation, as well as for the classification of unknown strains isolated from seafood.
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Species Differentiation of seafood spoilage and pathogenic gram negative bacteria by maldi tof mass fingerprinting
Journal of Proteome Research, 2010Co-Authors: Karola Böhme, Inmaculada C Fernandezno, Pilar Calomata, Jorge Barrosvelazquez, José Manuel Gallardo, Benito CañasAbstract:Species Differentiation is important for the early detection and identification of pathogenic and food-spoilage microorganisms that may be present in fish and seafood products. The main 26 Species ...
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Species Differentiation of seafood spoilage and pathogenic gram negative bacteria by maldi tof mass fingerprinting
Journal of Proteome Research, 2010Co-Authors: Karola Böhme, Inmaculada C Fernandezno, Pilar Calomata, Jorge Barrosvelazquez, José Manuel Gallardo, Benito CañasAbstract:Species Differentiation is important for the early detection and identification of pathogenic and food-spoilage microorganisms that may be present in fish and seafood products. The main 26 Species of seafood spoilage and pathogenic Gram-negative bacteria, including Aeromonas hydrophila, Acinetobacter baumanii, Pseudomonas spp., and Enterobacter spp. among others, were characterized by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) of low molecular weight proteins extracted from intact bacterial cells by a fast procedure. From the acquired spectra, a library of specific mass spectral fingerprints was constructed. To analyze spectral fingerprints, peaks in the mass range of 2000−10 000 Da were considered and representative mass lists of 10−35 peak masses were compiled. At least one unique biomarker peak was observed for each Species, and various genus-specific peaks were detected for genera Proteus, Providencia, Pseudomonas, Serratia, Shewanella, and Vibrio. Phyl...
Dania Richter - One of the best experts on this subject based on the ideXlab platform.
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absence of lyme disease spirochetes in larval ixodes ricinus ticks
Vector-borne and Zoonotic Diseases, 2012Co-Authors: Dania Richter, Alina Debski, Zdenek Hubalek, Franzrainer MatuschkaAbstract:Abstract To determine which kind of spirochete infects larval Ixodes ricinus, we examined questing larvae and larvae derived from engorged females for the presence of particular spirochetal DNA that permitted Species Differentiation. Borrelia miyamotoi was the sole spirochete detected in larval ticks sampled while questing on vegetation. Questing nymphal and adult ticks were infected mainly by Borrelia afzelii, whereas larval ticks resulting from engorged females of the same population were solely infected by B. miyamotoi. Since larvae acquire Lyme disease spirochetes within a few hours of attachment to an infected rodent, questing larvae in nature may have acquired Lyme disease spirochetes from an interrupted host contact. Even if transovarial transmission of Lyme disease spirochetes may occasionally occur, it seems to be an exceedingly rare event. No undisputable proof exists for vertical transmission of Lyme disease spirochetes, whereas B. miyamotoi appears to be readily passed between generations of v...
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Absence of Lyme disease spirochetes in larval Ixodes ricinus ticks.
Vector borne and zoonotic diseases (Larchmont N.Y.), 2011Co-Authors: Dania Richter, Alina Debski, Zdenek Hubalek, Franzrainer MatuschkaAbstract:Abstract To determine which kind of spirochete infects larval Ixodes ricinus, we examined questing larvae and larvae derived from engorged females for the presence of particular spirochetal DNA that permitted Species Differentiation. Borrelia miyamotoi was the sole spirochete detected in larval ticks sampled while questing on vegetation. Questing nymphal and adult ticks were infected mainly by Borrelia afzelii, whereas larval ticks resulting from engorged females of the same population were solely infected by B. miyamotoi. Since larvae acquire Lyme disease spirochetes within a few hours of attachment to an infected rodent, questing larvae in nature may have acquired Lyme disease spirochetes from an interrupted host contact. Even if transovarial transmission of Lyme disease spirochetes may occasionally occur, it seems to be an exceedingly rare event. No undisputable proof exists for vertical transmission of Lyme disease spirochetes, whereas B. miyamotoi appears to be readily passed between generations of v...
