The Experts below are selected from a list of 234 Experts worldwide ranked by ideXlab platform

Mladen Picer - One of the best experts on this subject based on the ideXlab platform.

  • Evaluation of modifications of the simple Spectrofluorometry method for estimating petroleum hydrocarbon levels in sea sediment samples
    Water Research, 1994
    Co-Authors: Mladen Picer, Vera Hocenski
    Abstract:

    Abstract The monitoring of petroleum hydrocarbons in the water environment very often involves complicated methods. There are many difficulties encountered in analyzing large numbers of samples in the monitoring programs. In such cases, a simpler, though less precise and specific, technique using inexpensive equipment may be more desirable. This idea was realized in an assessment of the petroleum hydrocarbon pollution of the Mediterranean Sea where the oil pollutant (dissolved and/or dispersed in sea water) was measured by Spectrofluorometry. This paper describes an analytical evaluation of three modifications of two simple Spectrofluorometry methods, used on numerous sediment samples collected from various areas of the Adriatic Sea.

  • Evaluation of modifications of the simple Spectrofluorometry method for estimating petroleum hydrocarbon levels in fresh and waste water samples
    Chemosphere, 1992
    Co-Authors: Mladen Picer, Nena Picer
    Abstract:

    Abstract The monitoring of petroleum hydrocarbons in the water environment is very often performed using complicated methods. There are many difficulties encountered in analyzing a long number of samples in the monitoring programme. In such cases, a simpler though less precise and specific technique using inexpensive equipment to monitor a large number of water samples, might be more desirable. This idea was realized in the assessment of the state of pollution of the Mediterranean Sea by petroleum hydrocarbons where the pollution of sea water with oil (dissolved and/or dispersed in sea water) was measured by Spectrofluorometry. The paper describes an analytical evaluation of three modifications of simple the Spectrofluorometry method, applied on numerous river and waste water samples collected from various areas of Croatia.

Giovanni Bottiroli - One of the best experts on this subject based on the ideXlab platform.

  • In vivo autofluorescence Spectrofluorometry of central serotonin.
    Journal of neuroscience methods, 2004
    Co-Authors: Francesco Crespi, Anna C Croce, Sabrina Fiorani, Barbara Masala, Christian Heidbreder, Giovanni Bottiroli
    Abstract:

    The autofluorescence properties of serotonin (5-HT) were investigated by light Spectrofluorometry in in vitro, ex vivo and in vivo experiments. Ex vivo samples were prepared from rat brain regions containing serotonin (5-HT) i.e. cortex, striatum, hippocampus. Rats were untreated (controls) or previously submitted to chronic behavioural or pharmacological treatments known to affect endogenous 5-HT levels. Autofluorescence analysis (excitation: 366 nm) on hippocampus homogenates supplied with exogenous 5-HT revealed spectral alterations attributable to changes of endogenous 5-HT levels. In vivo, real time fluorescence studies were performed via a 50 microm diameter optic fiber probe stereotaxically implanted into selected brain areas of anaesthetised rats treated with fluoxetine or 5-OH-tryptophan. All autofluorescence data were consistent with those obtained in parallel experiments performed with ex vivo or in vivo voltammetry, confirming that auto-fluorescence spectroscopy is a suitable technique for the direct assessment of fluorescent neurotransmitters. This is a reliable evidence of the in vivo application of spectroscopy together with optic fiber probe for in vivo, in situ and real time measurement of 5-HT in discrete brain areas.

  • autofluorescence Spectrofluorometry of central nervous system cns neuromediators
    Lasers in Surgery and Medicine, 2004
    Co-Authors: Francesco Crespi, Anna C Croce, Sabrina Fiorani, Barbara Masala, Christian Heidbreder, Giovanni Bottiroli
    Abstract:

    Background and Objectives Changes in the neurotransmitter 5-hydroxytriptamine (5-HT) are related to psychiatric diseases such as depression and anxiety. In this study, 5-HT autofluorescence properties were investigated in solution and in biological tissues. Study Design/Materials and Methods Spectrofluorometric characterization was performed on ex vivo samples (tissue sections, homogenates) of the 5-HT-rich brain region hippocampus from rats untreated or treated to affect endogenous 5-HT levels; in vivo, with a 50 ⊘ optic fiber probe positioned via stereotaxis. Results 5-HT exhibited minor excitation and emission bands at wavelengths longer than the well known excitation and emission bands in the UV region, 250–320 nm. Spectrofluorometric measurements under 366 nm excitation on homogenates supplied with 5-HT or belonging to treated rats revealed spectral alterations attributable to changes in the amount of 5-HT. Ex vivo and in vivo autofluorescence data were consistent with those obtained by conventional voltammetry. Conclusions Autofluorescence spectroscopy potential is confirmed as a suitable technique for the direct measurement of neurotransmitters. Lasers Surg. Med. 34:39–47, 2004. © 2004 Wiley-Liss, Inc.

Vera Hocenski - One of the best experts on this subject based on the ideXlab platform.

Nena Picer - One of the best experts on this subject based on the ideXlab platform.

  • Evaluation of modifications of the simple Spectrofluorometry method for estimating petroleum hydrocarbon levels in fresh and waste water samples
    Chemosphere, 1992
    Co-Authors: Mladen Picer, Nena Picer
    Abstract:

    Abstract The monitoring of petroleum hydrocarbons in the water environment is very often performed using complicated methods. There are many difficulties encountered in analyzing a long number of samples in the monitoring programme. In such cases, a simpler though less precise and specific technique using inexpensive equipment to monitor a large number of water samples, might be more desirable. This idea was realized in the assessment of the state of pollution of the Mediterranean Sea by petroleum hydrocarbons where the pollution of sea water with oil (dissolved and/or dispersed in sea water) was measured by Spectrofluorometry. The paper describes an analytical evaluation of three modifications of simple the Spectrofluorometry method, applied on numerous river and waste water samples collected from various areas of Croatia.

Anna C Croce - One of the best experts on this subject based on the ideXlab platform.

  • In vivo autofluorescence Spectrofluorometry of central serotonin.
    Journal of neuroscience methods, 2004
    Co-Authors: Francesco Crespi, Anna C Croce, Sabrina Fiorani, Barbara Masala, Christian Heidbreder, Giovanni Bottiroli
    Abstract:

    The autofluorescence properties of serotonin (5-HT) were investigated by light Spectrofluorometry in in vitro, ex vivo and in vivo experiments. Ex vivo samples were prepared from rat brain regions containing serotonin (5-HT) i.e. cortex, striatum, hippocampus. Rats were untreated (controls) or previously submitted to chronic behavioural or pharmacological treatments known to affect endogenous 5-HT levels. Autofluorescence analysis (excitation: 366 nm) on hippocampus homogenates supplied with exogenous 5-HT revealed spectral alterations attributable to changes of endogenous 5-HT levels. In vivo, real time fluorescence studies were performed via a 50 microm diameter optic fiber probe stereotaxically implanted into selected brain areas of anaesthetised rats treated with fluoxetine or 5-OH-tryptophan. All autofluorescence data were consistent with those obtained in parallel experiments performed with ex vivo or in vivo voltammetry, confirming that auto-fluorescence spectroscopy is a suitable technique for the direct assessment of fluorescent neurotransmitters. This is a reliable evidence of the in vivo application of spectroscopy together with optic fiber probe for in vivo, in situ and real time measurement of 5-HT in discrete brain areas.

  • autofluorescence Spectrofluorometry of central nervous system cns neuromediators
    Lasers in Surgery and Medicine, 2004
    Co-Authors: Francesco Crespi, Anna C Croce, Sabrina Fiorani, Barbara Masala, Christian Heidbreder, Giovanni Bottiroli
    Abstract:

    Background and Objectives Changes in the neurotransmitter 5-hydroxytriptamine (5-HT) are related to psychiatric diseases such as depression and anxiety. In this study, 5-HT autofluorescence properties were investigated in solution and in biological tissues. Study Design/Materials and Methods Spectrofluorometric characterization was performed on ex vivo samples (tissue sections, homogenates) of the 5-HT-rich brain region hippocampus from rats untreated or treated to affect endogenous 5-HT levels; in vivo, with a 50 ⊘ optic fiber probe positioned via stereotaxis. Results 5-HT exhibited minor excitation and emission bands at wavelengths longer than the well known excitation and emission bands in the UV region, 250–320 nm. Spectrofluorometric measurements under 366 nm excitation on homogenates supplied with 5-HT or belonging to treated rats revealed spectral alterations attributable to changes in the amount of 5-HT. Ex vivo and in vivo autofluorescence data were consistent with those obtained by conventional voltammetry. Conclusions Autofluorescence spectroscopy potential is confirmed as a suitable technique for the direct measurement of neurotransmitters. Lasers Surg. Med. 34:39–47, 2004. © 2004 Wiley-Liss, Inc.