Spectrophotometry

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Bosch C Ojeda - One of the best experts on this subject based on the ideXlab platform.

  • recent applications in derivative ultraviolet visible absorption Spectrophotometry 2009 2011 a review
    Microchemical Journal, 2013
    Co-Authors: Bosch C Ojeda, Sanchez F Rojas
    Abstract:

    Abstract Derivative Spectrophotometry (DS) has been introduced for the resolution of overlapping peaks. DS method has been widely used to enhance the signal and resolve the overlapped peak-signals due to its advantages in differentiating closely adjacent peaks, and identifying weak peaks obscured by sharp peaks. In this work, the analytical applications of derivative UV/VIS region absorption Spectrophotometry produced in the last 3 years (since 2009) are reviewed.

  • recent development in derivative ultraviolet visible absorption Spectrophotometry 2004 2008 a review
    Analytica Chimica Acta, 2009
    Co-Authors: Sanchez F Rojas, Bosch C Ojeda
    Abstract:

    Abstract Several techniques have been proposed for treatment of spectrophotometric data, with the objective of extracting a largest amount of analytical information from spectra composed of unresolved bands. Undoubtedly, a major success was achieved by derivative treatment of the absorbance curves-plotting of the first or a higher order mathematical derivative of absorbance against wavelength (d A /d λ ). Derivative Spectrophotometry has been applied to many chemical systems, such as pharmaceuticals, foods, cosmetics, and environmental samples. The instrumental development and analytical applications of derivative UV–vis regions absorption Spectrophotometry produced in the last 5 years (since 2004) are reviewed.

  • reviewrecent development in derivative ultraviolet visible absorption Spectrophotometry 2004 2008 a review
    Analytica Chimica Acta, 2009
    Co-Authors: Sanchez F Rojas, Bosch C Ojeda
    Abstract:

    Several techniques have been proposed for treatment of spectrophotometric data, with the objective of extracting a largest amount of analytical information from spectra composed of unresolved bands. Undoubtedly, a major success was achieved by derivative treatment of the absorbance curves-plotting of the first or a higher order mathematical derivative of absorbance against wavelength (dA/dλ). Derivative Spectrophotometry has been applied to many chemical systems, such as pharmaceuticals, foods, cosmetics, and environmental samples. The instrumental development and analytical applications of derivative UV–vis regions absorption Spectrophotometry produced in the last 5 years (since 2004) are reviewed.

  • recent developments in derivative ultraviolet visible absorption Spectrophotometry
    Analytica Chimica Acta, 2004
    Co-Authors: Bosch C Ojeda, Sanchez F Rojas
    Abstract:

    Abstract Derivative Spectrophotometry is an analytical technique of great utility for extracting both qualitative and quantitative information from spectra composed of unresolved bands, and for eliminating the effect of baseline shifts and baseline tilts. It consists of calculating and plotting one of the mathematical derivatives of a spectral curve. Thus, the information content of a spectrum is presented in a potentially more useful form, offering a convenient solution to a number of analytical problems, such as resolution of multi-component systems, removal of sample turbidity, matrix background and enhancement of spectral details. Derivative Spectrophotometry is now a reasonably priced standard feature of modern micro-computerized UV/Vis Spectrophotometry. The instrumental development and analytical applications of derivative UV/Vis regions absorption Spectrophotometry produced in the last 10 years (since 1994) are reviewed.

Sanchez F Rojas - One of the best experts on this subject based on the ideXlab platform.

  • recent applications in derivative ultraviolet visible absorption Spectrophotometry 2009 2011 a review
    Microchemical Journal, 2013
    Co-Authors: Bosch C Ojeda, Sanchez F Rojas
    Abstract:

    Abstract Derivative Spectrophotometry (DS) has been introduced for the resolution of overlapping peaks. DS method has been widely used to enhance the signal and resolve the overlapped peak-signals due to its advantages in differentiating closely adjacent peaks, and identifying weak peaks obscured by sharp peaks. In this work, the analytical applications of derivative UV/VIS region absorption Spectrophotometry produced in the last 3 years (since 2009) are reviewed.

  • recent development in derivative ultraviolet visible absorption Spectrophotometry 2004 2008 a review
    Analytica Chimica Acta, 2009
    Co-Authors: Sanchez F Rojas, Bosch C Ojeda
    Abstract:

    Abstract Several techniques have been proposed for treatment of spectrophotometric data, with the objective of extracting a largest amount of analytical information from spectra composed of unresolved bands. Undoubtedly, a major success was achieved by derivative treatment of the absorbance curves-plotting of the first or a higher order mathematical derivative of absorbance against wavelength (d A /d λ ). Derivative Spectrophotometry has been applied to many chemical systems, such as pharmaceuticals, foods, cosmetics, and environmental samples. The instrumental development and analytical applications of derivative UV–vis regions absorption Spectrophotometry produced in the last 5 years (since 2004) are reviewed.

  • reviewrecent development in derivative ultraviolet visible absorption Spectrophotometry 2004 2008 a review
    Analytica Chimica Acta, 2009
    Co-Authors: Sanchez F Rojas, Bosch C Ojeda
    Abstract:

    Several techniques have been proposed for treatment of spectrophotometric data, with the objective of extracting a largest amount of analytical information from spectra composed of unresolved bands. Undoubtedly, a major success was achieved by derivative treatment of the absorbance curves-plotting of the first or a higher order mathematical derivative of absorbance against wavelength (dA/dλ). Derivative Spectrophotometry has been applied to many chemical systems, such as pharmaceuticals, foods, cosmetics, and environmental samples. The instrumental development and analytical applications of derivative UV–vis regions absorption Spectrophotometry produced in the last 5 years (since 2004) are reviewed.

  • recent developments in derivative ultraviolet visible absorption Spectrophotometry
    Analytica Chimica Acta, 2004
    Co-Authors: Bosch C Ojeda, Sanchez F Rojas
    Abstract:

    Abstract Derivative Spectrophotometry is an analytical technique of great utility for extracting both qualitative and quantitative information from spectra composed of unresolved bands, and for eliminating the effect of baseline shifts and baseline tilts. It consists of calculating and plotting one of the mathematical derivatives of a spectral curve. Thus, the information content of a spectrum is presented in a potentially more useful form, offering a convenient solution to a number of analytical problems, such as resolution of multi-component systems, removal of sample turbidity, matrix background and enhancement of spectral details. Derivative Spectrophotometry is now a reasonably priced standard feature of modern micro-computerized UV/Vis Spectrophotometry. The instrumental development and analytical applications of derivative UV/Vis regions absorption Spectrophotometry produced in the last 10 years (since 1994) are reviewed.

Mohamad Mahani - One of the best experts on this subject based on the ideXlab platform.

  • micro rna detection based on fluorescence resonance energy transfer of dna carbon quantum dots probes
    Analytical Biochemistry, 2017
    Co-Authors: Faeze Khakbaz, Mohamad Mahani
    Abstract:

    Carbon quantum dots have been proposed as an effective platform for miRNA detection. Carbon dots were synthesized by citric acid. The synthesized dots were characterized by dynamic light scattering, UV-Vis Spectrophotometry, spectrofluorimetry, transmission electron microscopy and FT-IR Spectrophotometry. The fluorescence quantum yield of the synthesized dots was determined using quinine sulfate as the standard. The FAM-labeled single stranded DNA, as sensing element, was adsorbed on dots by π-π interaction. The quenching of the dots fluorescence due to fluorescence resonance energy transfer (FRET) was used for mir 9-1 detection. In the presence of the complementary miRNA, the FRET did not take place and the fluorescence was recovered.

Faeze Khakbaz - One of the best experts on this subject based on the ideXlab platform.

  • micro rna detection based on fluorescence resonance energy transfer of dna carbon quantum dots probes
    Analytical Biochemistry, 2017
    Co-Authors: Faeze Khakbaz, Mohamad Mahani
    Abstract:

    Carbon quantum dots have been proposed as an effective platform for miRNA detection. Carbon dots were synthesized by citric acid. The synthesized dots were characterized by dynamic light scattering, UV-Vis Spectrophotometry, spectrofluorimetry, transmission electron microscopy and FT-IR Spectrophotometry. The fluorescence quantum yield of the synthesized dots was determined using quinine sulfate as the standard. The FAM-labeled single stranded DNA, as sensing element, was adsorbed on dots by π-π interaction. The quenching of the dots fluorescence due to fluorescence resonance energy transfer (FRET) was used for mir 9-1 detection. In the presence of the complementary miRNA, the FRET did not take place and the fluorescence was recovered.

R Prashanthi - One of the best experts on this subject based on the ideXlab platform.

  • assay of tolperisone by extractive Spectrophotometry
    Asian Journal of Research in Chemistry, 2011
    Co-Authors: K Raghavi, M Shaiba, V Jagathi, M Sindhura, R Prashanthi
    Abstract:

    Two simple and sensitive visible spectrophotometric methods have been developed for the estimation of Tolperisone in pure and pharmaceutical dosage forms. Method A is based on co-ordinate complex formation between the drug and cobalt thiocynate (630 nm) and method B is based on charge transfer complex formation between drug and 2,3 di-chloro-5-6-dicyano 1–4,benzoquinone (DDQ 430 nm). The absorbance are measured against the corresponding reagent blanks. The methods have been statistically evaluated and found to be precise and accurate.