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Bernard Robaire - One of the best experts on this subject based on the ideXlab platform.
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effects of ageing on Spermatozoal chromatin and its sensitivity to in vivo and in vitro oxidative challenge in the brown norway rat
Human Reproduction, 2006Co-Authors: Ekaterina V Zubkova, Bernard RobaireAbstract:BACKGROUND: The goals of our study were to examine chromatin packaging and integrity in Spermatozoa taken from the caput and cauda epididymides of young (4-month-old) and old (21-month-old) Brown Norway rats and to assess whether Spermatozoal sensitivity to oxidative treatments is altered with age. METHODS: Oxidative treatments consisted of (i) in vivo oxidative challenge by systemic administration of the glutathione-depleting drug L-buthionine-[S,R]-sulphoximine (BSO) and (ii) in vitro oxidative challenge by incubating collected Spermatozoa with hydrogen peroxide (H 2 O 2 ). Chromatin parameters assessed included quantification of thiols, nuclear chromomycin A3 (CMA3) penetration, DNA breaks by TdT (terminal deoxynucleotidyl transferase)-mediated dUDP nick-end labelling (TUNEL) and ease of DNA dissociation by acridine orange (AO) staining. RESULTS: In Spermatozoa from older rats, we found decreases in thiols, CMA3 penetration and the percentage of cells that undergo DNA dissociation. Administration of BSO had oxidizing effects on the thiol groups. It also decreased CMA3 penetration and DNA dissociation and increased TUNEL staining. Furthermore, BSO treatment sensitized cauda epididymidis Spermatozoa, from older animals, to H 2 O 2 . CONCLUSIONS: Overall, we show that Spermatozoa from older rats have altered chromatin packaging and integrity and that Spermatozoa from the cauda epididymidis are more responsive to combined in vivo and in vitro oxidative challenge than Spermatozoa from young rats.
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effect of glutathione depletion on antioxidant enzymes in the epididymis seminal vesicles and liver and on Spermatozoa motility in the aging brown norway rat
Biology of Reproduction, 2004Co-Authors: Ekaterina V Zubkova, Bernard RobaireAbstract:Reactive oxygen species (ROS) play a role in male infertility, where excessive amounts impair Spermatozoal motility. Epididymal antioxidant enzymes protect Spermatozoa from oxidative damage in the epididymal lumen. Antioxidant secretions from the seminal vesicle protect Spermatozoa after ejaculation. As it is known that with age there is increased generation of ROS, the goals of this study were to determine how aging affects the response of antioxidant enzymes in the epididymis, seminal vesicles, and liver to L-buthionine-S,R-sulfoximine (BSO) mediated glutathione (GSH) depletion, and to examine the impact of GSH depletion on motility parameters of Spermatozoa from the cauda epididymidis in young (4-mo-old) and old (21-mo-old) rats. Levels of GSH and glutathione disulfide (GSSG), as well as activities of glutathione peroxidase, glutathione reductase, catalase, and superoxide dismutase, were measured in the caput, corpus and cauda epididymidis, seminal vesicles, and liver. Spermatozoal motility was assessed by computer-assisted sperm analysis. Significant age-related changes in antioxidant enzyme activities were found in the liver and cauda epididymidis. Glutathione depletion clearly affected tissues in both young and old. The compounding effect of age was most evident in the cauda epididymidis, seminal vesicles, and liver, where antioxidant enzyme activities changed significantly. Additionally, Spermatozoa motility was adversely affected after BSO treatment in both age groups, but significantly more so in older animals. In summary, the male reproductive tissues and liver undergo age-related changes in antioxidant enzyme activities and in their response to GSH depletion. aging, epididymis, seminal vesicles, sperm, stress
Rajesh S Mathur - One of the best experts on this subject based on the ideXlab platform.
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presence of platelet activating factor in squirrel monkey saimiri boliviensis Spermatozoa seasonal differences
American Journal of Primatology, 1998Co-Authors: William E Roudebush, Rajesh S MathurAbstract:Platelet-activating factor (1-O-alkyl-2-acetyl-sn-glycero-3-phosphorylcholine) (PAF) is a potent signaling phospholipid which has pleiotropic biological properties in addition to platelet activation. PAF has been detected in the Spermatozoa in a number of species. The concentration of PAF is inversely related to human Spermatozoal quality. There are no reports on the presence of PAF in nonhuman primate Spermatozoa. Therefore, the primary objective of this study was to determine if PAF is present in the Spermatozoa from the squirrel monkey (which is a seasonal breeder). A second objective was to determine if PAF levels change from the breeding to the nonbreeding season. Endogenous lipids were extracted from mature Bolivian squirrel monkeys (Saimiri boliviensis) Spermatozoa and assayed for the presence of PAF by [125I] radioimmunoassay. PAF was detected in all samples assayed. PAF levels were significantly higher (P< 0.01) during the breeding season (mean: 3.58 ng/106 Spermatozoa) than the nonbreeding season (mean: 0.76 ng/106 Spermatozoa). The data demonstrate that PAF is present in squirrel monkey Spermatozoa, with higher levels found during the breeding season. Additional studies are warranted to elucidate the role of PAF in Spermatozoa function. Am. J. Primatol. 45:301–305, 1998. © 1998 Wiley-Liss, Inc.
Ekaterina V Zubkova - One of the best experts on this subject based on the ideXlab platform.
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effects of ageing on Spermatozoal chromatin and its sensitivity to in vivo and in vitro oxidative challenge in the brown norway rat
Human Reproduction, 2006Co-Authors: Ekaterina V Zubkova, Bernard RobaireAbstract:BACKGROUND: The goals of our study were to examine chromatin packaging and integrity in Spermatozoa taken from the caput and cauda epididymides of young (4-month-old) and old (21-month-old) Brown Norway rats and to assess whether Spermatozoal sensitivity to oxidative treatments is altered with age. METHODS: Oxidative treatments consisted of (i) in vivo oxidative challenge by systemic administration of the glutathione-depleting drug L-buthionine-[S,R]-sulphoximine (BSO) and (ii) in vitro oxidative challenge by incubating collected Spermatozoa with hydrogen peroxide (H 2 O 2 ). Chromatin parameters assessed included quantification of thiols, nuclear chromomycin A3 (CMA3) penetration, DNA breaks by TdT (terminal deoxynucleotidyl transferase)-mediated dUDP nick-end labelling (TUNEL) and ease of DNA dissociation by acridine orange (AO) staining. RESULTS: In Spermatozoa from older rats, we found decreases in thiols, CMA3 penetration and the percentage of cells that undergo DNA dissociation. Administration of BSO had oxidizing effects on the thiol groups. It also decreased CMA3 penetration and DNA dissociation and increased TUNEL staining. Furthermore, BSO treatment sensitized cauda epididymidis Spermatozoa, from older animals, to H 2 O 2 . CONCLUSIONS: Overall, we show that Spermatozoa from older rats have altered chromatin packaging and integrity and that Spermatozoa from the cauda epididymidis are more responsive to combined in vivo and in vitro oxidative challenge than Spermatozoa from young rats.
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effect of glutathione depletion on antioxidant enzymes in the epididymis seminal vesicles and liver and on Spermatozoa motility in the aging brown norway rat
Biology of Reproduction, 2004Co-Authors: Ekaterina V Zubkova, Bernard RobaireAbstract:Reactive oxygen species (ROS) play a role in male infertility, where excessive amounts impair Spermatozoal motility. Epididymal antioxidant enzymes protect Spermatozoa from oxidative damage in the epididymal lumen. Antioxidant secretions from the seminal vesicle protect Spermatozoa after ejaculation. As it is known that with age there is increased generation of ROS, the goals of this study were to determine how aging affects the response of antioxidant enzymes in the epididymis, seminal vesicles, and liver to L-buthionine-S,R-sulfoximine (BSO) mediated glutathione (GSH) depletion, and to examine the impact of GSH depletion on motility parameters of Spermatozoa from the cauda epididymidis in young (4-mo-old) and old (21-mo-old) rats. Levels of GSH and glutathione disulfide (GSSG), as well as activities of glutathione peroxidase, glutathione reductase, catalase, and superoxide dismutase, were measured in the caput, corpus and cauda epididymidis, seminal vesicles, and liver. Spermatozoal motility was assessed by computer-assisted sperm analysis. Significant age-related changes in antioxidant enzyme activities were found in the liver and cauda epididymidis. Glutathione depletion clearly affected tissues in both young and old. The compounding effect of age was most evident in the cauda epididymidis, seminal vesicles, and liver, where antioxidant enzyme activities changed significantly. Additionally, Spermatozoa motility was adversely affected after BSO treatment in both age groups, but significantly more so in older animals. In summary, the male reproductive tissues and liver undergo age-related changes in antioxidant enzyme activities and in their response to GSH depletion. aging, epididymis, seminal vesicles, sperm, stress
Jing Chen - One of the best experts on this subject based on the ideXlab platform.
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mesenchytraeus monodiverticulus sp nov annelida clitellata enchytraeidae from changbai mountain with a key to mesenchytraeus with enlarged chaetae
Proceedings of the Biological Society of Washington, 2012Co-Authors: Qi Shen, Jing ChenAbstract:Abstract Mesenchytraeus monodiverticulus, new species, (Clitellata: Enchytraeidae) is described based on live and preserved specimens from Changbai Mountain. It is diagnosed by possessing enlarged ventral chaetae in V–VI (4–5 per bundle), nephridium holding solid unlobed postseptale, spermathecae possessing only one diverticulum and attached entally to esophagus in VII, atria cylindrical without prostate glands, and the presence of regular Spermatozoal bundles in seminal vesicle (XI–XIII) with the heads of Spermatozoa arranged in both ends orderly. The major differences between M. monodiverticulus and its congeners are discussed. A key to species of Mesenchytraeus that possess enlarged chaetae is provided.
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mesenchytraeus monodiverticulus sp nov annelida clitellata enchytraeidae from changbai mountain with a key to mesenchytraeus with enlarged chaetae
Proceedings of the Biological Society of Washington, 2012Co-Authors: Qi Shen, Jing ChenAbstract:Abstract Mesenchytraeus monodiverticulus, new species, (Clitellata: Enchytraeidae) is described based on live and preserved specimens from Changbai Mountain. It is diagnosed by possessing enlarged ventral chaetae in V–VI (4–5 per bundle), nephridium holding solid unlobed postseptale, spermathecae possessing only one diverticulum and attached entally to esophagus in VII, atria cylindrical without prostate glands, and the presence of regular Spermatozoal bundles in seminal vesicle (XI–XIII) with the heads of Spermatozoa arranged in both ends orderly. The major differences between M. monodiverticulus and its congeners are discussed. A key to species of Mesenchytraeus that possess enlarged chaetae is provided.
Qi Shen - One of the best experts on this subject based on the ideXlab platform.
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mesenchytraeus monodiverticulus sp nov annelida clitellata enchytraeidae from changbai mountain with a key to mesenchytraeus with enlarged chaetae
Proceedings of the Biological Society of Washington, 2012Co-Authors: Qi Shen, Jing ChenAbstract:Abstract Mesenchytraeus monodiverticulus, new species, (Clitellata: Enchytraeidae) is described based on live and preserved specimens from Changbai Mountain. It is diagnosed by possessing enlarged ventral chaetae in V–VI (4–5 per bundle), nephridium holding solid unlobed postseptale, spermathecae possessing only one diverticulum and attached entally to esophagus in VII, atria cylindrical without prostate glands, and the presence of regular Spermatozoal bundles in seminal vesicle (XI–XIII) with the heads of Spermatozoa arranged in both ends orderly. The major differences between M. monodiverticulus and its congeners are discussed. A key to species of Mesenchytraeus that possess enlarged chaetae is provided.
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mesenchytraeus monodiverticulus sp nov annelida clitellata enchytraeidae from changbai mountain with a key to mesenchytraeus with enlarged chaetae
Proceedings of the Biological Society of Washington, 2012Co-Authors: Qi Shen, Jing ChenAbstract:Abstract Mesenchytraeus monodiverticulus, new species, (Clitellata: Enchytraeidae) is described based on live and preserved specimens from Changbai Mountain. It is diagnosed by possessing enlarged ventral chaetae in V–VI (4–5 per bundle), nephridium holding solid unlobed postseptale, spermathecae possessing only one diverticulum and attached entally to esophagus in VII, atria cylindrical without prostate glands, and the presence of regular Spermatozoal bundles in seminal vesicle (XI–XIII) with the heads of Spermatozoa arranged in both ends orderly. The major differences between M. monodiverticulus and its congeners are discussed. A key to species of Mesenchytraeus that possess enlarged chaetae is provided.
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Mesenchytraeus monodiverticulus sp nov (Annelida: Clitellata: Enchytraeidae) from Changbai Mountain, with a key to Mesenchytraeus with enlarged chaetae
2012Co-Authors: Qi Shen, Chen Jing, Xie Zhicai, Xie, Zc Author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol Biotechnol, Wuhan 4 China.Abstract:Mesenchytraeus monodiverticulus, new species, (Clitellata: Enchytraeidae) is described based on live and preserved specimens from Changbai Mountain. It is diagnosed by possessing enlarged ventral chaetae in V-VI (4-5 per bundle), nephridium holding solid unlobed postseptale, spermathecae possessing only one diverticulum and attached entally to esophagus in VII, atria cylindrical without prostate glands, and the presence of regular Spermatozoal bundles in seminal vesicle (XI-XIII) with the heads of Spermatozoa arranged in both ends orderly. The major differences between M. monodiyerticulus and its congeners are discussed. A key to species of Mesenchytraeus that possess enlarged chaetae is provided.Mesenchytraeus monodiverticulus, new species, (Clitellata: Enchytraeidae) is described based on live and preserved specimens from Changbai Mountain. It is diagnosed by possessing enlarged ventral chaetae in V-VI (4-5 per bundle), nephridium holding solid unlobed postseptale, spermathecae possessing only one diverticulum and attached entally to esophagus in VII, atria cylindrical without prostate glands, and the presence of regular Spermatozoal bundles in seminal vesicle (XI-XIII) with the heads of Spermatozoa arranged in both ends orderly. The major differences between M. monodiyerticulus and its congeners are discussed. A key to species of Mesenchytraeus that possess enlarged chaetae is provided
