The Experts below are selected from a list of 8043 Experts worldwide ranked by ideXlab platform
I Friedberg - One of the best experts on this subject based on the ideXlab platform.
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Spheroplast derived membrane vesicles from rhodobacter capsulatus cells catalyzing nucleotide transport
Archives of Biochemistry and Biophysics, 1991Co-Authors: Chanoch Carmeli, Y Lifshitz, I FriedbergAbstract:Rodobacter capsulatus cells, which were cultured anaerobically in high light intensity, had fewer foldings in the cytoplasmic membrane than those which were grown in lower light intensities. Spheroplast-derived membrane fractions obtained from cells cultured under high light intensity contained a high yield of large right-side-out membrane vesicles. The right-side-out vesicles catalyzed reversible light-induced proton efflux as did intact cells. Nucleotide transport activity was also catalyzed by these membrane vesicles. This activity was indirectly monitored by measurement of photophosphorylation or hydrolysis of externally added diphospho- and triphosphonucleosides. These enzymatic activities occur inside the cytoplasmic membrane of Spheroplasts and membrane vesicles and therefore require the transport of the externally added reagents. The indirect measurements of transport were complemented by the demonstration of direct uptake of radiolabeled nucleotides into the membrane vesicles. These data support the suggestion that a nucleotide transporter located in the cytoplasmic membrane of R. capsulatus bacteria mediates these activities.
Toshio Fukuda - One of the best experts on this subject based on the ideXlab platform.
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application of environmental scanning electron microscope nanomanipulation system on Spheroplast yeast cells surface observation
Scanning, 2017Co-Authors: Maryam Alsadat Rad, Mohd Ridzuan Ahmad, Masahiro Nakajima, Seiji Kojima, Michio Homma, Toshio FukudaAbstract:The preparation and observations of Spheroplast W303 cells are described with Environmental Scanning Electron Microscope (ESEM). The Spheroplasting conversion was successfully confirmed qualitatively, by the evaluation of the morphological change between the normal W303 cells and the Spheroplast W303 cells, and quantitatively, by determining the Spheroplast conversion percentage based on the OD800 absorbance data. From the optical microscope observations as expected, the normal cells had an oval shape whereas Spheroplast cells resemble a spherical shape. This was also confirmed under four different mediums, that is, yeast peptone-dextrose (YPD), sterile water, sorbitol-EDTA-sodium citrate buffer (SCE), and sorbitol-Tris-Hcl-CaCl2 (CaS). It was also observed that the SCE and CaS mediums had a higher number of Spheroplast cells as compared to the YPD and sterile water mediums. The OD800 absorbance data also showed that the whole W303 cells were fully converted to the Spheroplast cells after about 15 minutes. The observations of the normal and the Spheroplast W303 cells were then performed under an environmental scanning electron microscope (ESEM). The normal cells showed a smooth cell surface whereas the Spheroplast cells had a bleb-like surface after the loss of its integrity when removing the cell wall.
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A Study of the Spheroplast Observations for W303 Single Cells under Environmental-SEM
2008 International Symposium on Micro-NanoMechatronics and Human Science, 2008Co-Authors: Mohd Ridzuan Ahmad, Masahiro Nakajima, Michio Homma, Seiji Kolima, Toshio FukudaAbstract:In this paper, the preparation and observations of the Spheroplast W303 single cells are described. Spheroplasting which is a process for removing a cell wall from a whole cell is often required for many applications. The successful of the Spheroplasting process were confirmed both qualitatively, i.e. the evaluation of the morphological change between the whole W303 cells and the Spheroplast W303 cells, and quantitatively, i.e. determination of the Spheroplast conversion percentage based on the OD800 absorbance data. It is expected that under optical microscope observations, whole cells appear an oval shape whereas Spheroplast cells resemble a spherical shape. This expectation was proved in our experiment. We have compared the shape of the whole and the Spheroplast of W303 cells inside four mediums, i.e. yeast peptone-dextrose (YPD), sterile water, sorbitol-EDTA-sodium citrate buffer (SCE) and sorbitol-Tris-Hcl-CaCl2 (CaS). The whole W303 cells exhibited an oval shape inside all mediums. On the other hand, a spherical shape was clearly observed for Spheroplast W303 cells inside all mediums where the SCE and CaS mediums have a higher number of observed Spheroplast cells as compared to the YPD and sterile water mediums. The OD800 absorbance data also revealed that the whole W303 cells were fully converted to the Spheroplast cells after about 15 minutes of the Spheroplasting process. The observations of the whole and the Spheroplast W303 cells were then performed under an environmental scanning electron microscope (ESEM).
Chanoch Carmeli - One of the best experts on this subject based on the ideXlab platform.
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Spheroplast derived membrane vesicles from rhodobacter capsulatus cells catalyzing nucleotide transport
Archives of Biochemistry and Biophysics, 1991Co-Authors: Chanoch Carmeli, Y Lifshitz, I FriedbergAbstract:Rodobacter capsulatus cells, which were cultured anaerobically in high light intensity, had fewer foldings in the cytoplasmic membrane than those which were grown in lower light intensities. Spheroplast-derived membrane fractions obtained from cells cultured under high light intensity contained a high yield of large right-side-out membrane vesicles. The right-side-out vesicles catalyzed reversible light-induced proton efflux as did intact cells. Nucleotide transport activity was also catalyzed by these membrane vesicles. This activity was indirectly monitored by measurement of photophosphorylation or hydrolysis of externally added diphospho- and triphosphonucleosides. These enzymatic activities occur inside the cytoplasmic membrane of Spheroplasts and membrane vesicles and therefore require the transport of the externally added reagents. The indirect measurements of transport were complemented by the demonstration of direct uptake of radiolabeled nucleotides into the membrane vesicles. These data support the suggestion that a nucleotide transporter located in the cytoplasmic membrane of R. capsulatus bacteria mediates these activities.
Maryam Alsadat Rad - One of the best experts on this subject based on the ideXlab platform.
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application of environmental scanning electron microscope nanomanipulation system on Spheroplast yeast cells surface observation
Scanning, 2017Co-Authors: Maryam Alsadat Rad, Mohd Ridzuan Ahmad, Masahiro Nakajima, Seiji Kojima, Michio Homma, Toshio FukudaAbstract:The preparation and observations of Spheroplast W303 cells are described with Environmental Scanning Electron Microscope (ESEM). The Spheroplasting conversion was successfully confirmed qualitatively, by the evaluation of the morphological change between the normal W303 cells and the Spheroplast W303 cells, and quantitatively, by determining the Spheroplast conversion percentage based on the OD800 absorbance data. From the optical microscope observations as expected, the normal cells had an oval shape whereas Spheroplast cells resemble a spherical shape. This was also confirmed under four different mediums, that is, yeast peptone-dextrose (YPD), sterile water, sorbitol-EDTA-sodium citrate buffer (SCE), and sorbitol-Tris-Hcl-CaCl2 (CaS). It was also observed that the SCE and CaS mediums had a higher number of Spheroplast cells as compared to the YPD and sterile water mediums. The OD800 absorbance data also showed that the whole W303 cells were fully converted to the Spheroplast cells after about 15 minutes. The observations of the normal and the Spheroplast W303 cells were then performed under an environmental scanning electron microscope (ESEM). The normal cells showed a smooth cell surface whereas the Spheroplast cells had a bleb-like surface after the loss of its integrity when removing the cell wall.
Y Lifshitz - One of the best experts on this subject based on the ideXlab platform.
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Spheroplast derived membrane vesicles from rhodobacter capsulatus cells catalyzing nucleotide transport
Archives of Biochemistry and Biophysics, 1991Co-Authors: Chanoch Carmeli, Y Lifshitz, I FriedbergAbstract:Rodobacter capsulatus cells, which were cultured anaerobically in high light intensity, had fewer foldings in the cytoplasmic membrane than those which were grown in lower light intensities. Spheroplast-derived membrane fractions obtained from cells cultured under high light intensity contained a high yield of large right-side-out membrane vesicles. The right-side-out vesicles catalyzed reversible light-induced proton efflux as did intact cells. Nucleotide transport activity was also catalyzed by these membrane vesicles. This activity was indirectly monitored by measurement of photophosphorylation or hydrolysis of externally added diphospho- and triphosphonucleosides. These enzymatic activities occur inside the cytoplasmic membrane of Spheroplasts and membrane vesicles and therefore require the transport of the externally added reagents. The indirect measurements of transport were complemented by the demonstration of direct uptake of radiolabeled nucleotides into the membrane vesicles. These data support the suggestion that a nucleotide transporter located in the cytoplasmic membrane of R. capsulatus bacteria mediates these activities.