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Jörg Reimann - One of the best experts on this subject based on the ideXlab platform.

  • 16s like rdna sequence and phylogenetic position of the diplomonad Spironucleus muris lavier 1936
    European Journal of Protistology, 1996
    Co-Authors: Jurgen Branke, Manfred Berchtold, Alfred Breunig, Helmut König, Jörg Reimann
    Abstract:

    Summary The sequence of the small subunit (SSU) rDNA gene of the facultative pathogenic flagellate Spironucleus muris has been determined. This flagellate belongs to the class Diplomonadida. We will set out its phylogenetic position within the eukaryotic tree according to its morphological characteristics. Cells of Spironucleus muris were enriched from the digestive tract of immunodeficient mice and the DNA was extracted. After sequencing the SSU rDNA insert (1206 bp), we carried out a comparative sequence analysis with accessible SSU rRNA sequences from related eukaryotes. Escherichia coli and Methanospirilium hungatei were used as outgroups. Spironucleus muris shows strongest structural similarity (73.2%) to the parasitic flagellate Hexamita inflata (class Diplomonadida), which originated very early in the eukaryotic tree.

  • 16s like rdna sequence and phylogenetic position of the diplomonad Spironucleus muris lavier 1936
    European Journal of Protistology, 1996
    Co-Authors: Jurgen Branke, Manfred Berchtold, Alfred Breunig, Helmut König, Jörg Reimann
    Abstract:

    Summary The sequence of the small subunit (SSU) rDNA gene of the facultative pathogenic flagellate Spironucleus muris has been determined. This flagellate belongs to the class Diplomonadida. We will set out its phylogenetic position within the eukaryotic tree according to its morphological characteristics. Cells of Spironucleus muris were enriched from the digestive tract of immunodeficient mice and the DNA was extracted. After sequencing the SSU rDNA insert (1206 bp), we carried out a comparative sequence analysis with accessible SSU rRNA sequences from related eukaryotes. Escherichia coli and Methanospirilium hungatei were used as outgroups. Spironucleus muris shows strongest structural similarity (73.2%) to the parasitic flagellate Hexamita inflata (class Diplomonadida), which originated very early in the eukaryotic tree.

Edward L. Jarroll - One of the best experts on this subject based on the ideXlab platform.

  • in vitro excystation of Spironucleus muris
    Journal of Eukaryotic Microbiology, 1996
    Co-Authors: Břetislav Koudela, Craig D Karr, Ernest A Meyer, Barbara P Meyer, Edward L. Jarroll
    Abstract:

    In vitro excystation of Spironucleus muris cysts, purified by sequential sucrose and Percoll gradients from mouse feces, was studied. Three in vitro excystation procedures, used for Giardia, were assessed to determine the most useful method. Excystation was monitored by light microscopy and subsequently characterized by transmission and scanning electron microscopy. Spironucleus muris excysted routinely at a level greater than 90% when induced in Hanks' balanced salt solution containing sodium bicarbonate at pH 2.0 and transferred to Tyrodes' salt solution as an excystation medium. Similarly, high rates of excystation were recorded after induction of S. muris cysts in 0.1 M potassium phosphate buffer (pH 7.0) with sodium bicarbonate and excystation in trypticase-yeast extract-iron medium (TYI medium) or phosphate-buffered saline. A lower rate and percentage of excystation were observed after induction of S. muris cysts in an aqueous hydrochloric acid solution (pH 2.0) followed by excystation in TYI medium. All excystation methods produced extremely active S. muris trophozoites with normal morphology. Nonexcysting S. muris cysts have a wall composed of an outer fibrous and an inner membranous portion. Following induction, numerous vesicles appeared in the peritrophic space. Excystation began by the cyst wall opening at one pole, and the anterior part of the trophozoite protruding from the cyst wall. The trophozoite emerged progressively from the cyst wall and the empty cyst wall appeared to collapse. Excysted trophozoites exhibited normal morphological features of S. muris trophozoites isolated from the mouse intestine.

  • in vitro excystation of Spironucleus muris
    Journal of Eukaryotic Microbiology, 1996
    Co-Authors: Břetislav Koudela, Craig D Karr, Ernest A Meyer, Barbara P Meyer, Edward L. Jarroll
    Abstract:

    In vitro excystation of Spironucleus muris cysts, purified by sequential sucrose and Percoll gradients from mouse feces, was studied. Three in vitro excystation procedures, used for Giardia, were assessed to determine the most useful method. Excystation was monitored by light microscopy and subsequently characterized by transmission and scanning electron microscopy. Spironucleus muris excysted routinely at a level greater than 90% when induced in Hanks' balanced salt solution containing sodium bicarbonate at pH 2.0 and transferred to Tyrodes' salt solution as an excystation medium. Similarly, high rates of excystation were recorded after induction of S. muris cysts in 0.1 M potassium phosphate buffer (pH 7.0) with sodium bicarbonate and excystation in trypticase-yeast extract-iron medium (TYI medium) or phosphate-buffered saline. A lower rate and percentage of excystation were observed after induction of S. muris cysts in an aqueous hydrochloric acid solution (pH 2.0) followed by excystation in TYI medium. All excystation methods produced extremely active S. muris trophozoites with normal morphology. Nonexcysting S. muris cysts have a wall composed of an outer fibrous and an inner membranous portion. Following induction, numerous vesicles appeared in the peritrophic space. Excystation began by the cyst wall opening at one pole, and the anterior part of the trophozoite protruding from the cyst wall. The trophozoite emerged progressively from the cyst wall and the empty cyst wall appeared to collapse. Excysted trophozoites exhibited normal morphological features of S. muris trophozoites isolated from the mouse intestine.

Jurgen Branke - One of the best experts on this subject based on the ideXlab platform.

  • 16s like rdna sequence and phylogenetic position of the diplomonad Spironucleus muris lavier 1936
    European Journal of Protistology, 1996
    Co-Authors: Jurgen Branke, Manfred Berchtold, Alfred Breunig, Helmut König, Jörg Reimann
    Abstract:

    Summary The sequence of the small subunit (SSU) rDNA gene of the facultative pathogenic flagellate Spironucleus muris has been determined. This flagellate belongs to the class Diplomonadida. We will set out its phylogenetic position within the eukaryotic tree according to its morphological characteristics. Cells of Spironucleus muris were enriched from the digestive tract of immunodeficient mice and the DNA was extracted. After sequencing the SSU rDNA insert (1206 bp), we carried out a comparative sequence analysis with accessible SSU rRNA sequences from related eukaryotes. Escherichia coli and Methanospirilium hungatei were used as outgroups. Spironucleus muris shows strongest structural similarity (73.2%) to the parasitic flagellate Hexamita inflata (class Diplomonadida), which originated very early in the eukaryotic tree.

  • 16s like rdna sequence and phylogenetic position of the diplomonad Spironucleus muris lavier 1936
    European Journal of Protistology, 1996
    Co-Authors: Jurgen Branke, Manfred Berchtold, Alfred Breunig, Helmut König, Jörg Reimann
    Abstract:

    Summary The sequence of the small subunit (SSU) rDNA gene of the facultative pathogenic flagellate Spironucleus muris has been determined. This flagellate belongs to the class Diplomonadida. We will set out its phylogenetic position within the eukaryotic tree according to its morphological characteristics. Cells of Spironucleus muris were enriched from the digestive tract of immunodeficient mice and the DNA was extracted. After sequencing the SSU rDNA insert (1206 bp), we carried out a comparative sequence analysis with accessible SSU rRNA sequences from related eukaryotes. Escherichia coli and Methanospirilium hungatei were used as outgroups. Spironucleus muris shows strongest structural similarity (73.2%) to the parasitic flagellate Hexamita inflata (class Diplomonadida), which originated very early in the eukaryotic tree.

Manfred Berchtold - One of the best experts on this subject based on the ideXlab platform.

  • 16s like rdna sequence and phylogenetic position of the diplomonad Spironucleus muris lavier 1936
    European Journal of Protistology, 1996
    Co-Authors: Jurgen Branke, Manfred Berchtold, Alfred Breunig, Helmut König, Jörg Reimann
    Abstract:

    Summary The sequence of the small subunit (SSU) rDNA gene of the facultative pathogenic flagellate Spironucleus muris has been determined. This flagellate belongs to the class Diplomonadida. We will set out its phylogenetic position within the eukaryotic tree according to its morphological characteristics. Cells of Spironucleus muris were enriched from the digestive tract of immunodeficient mice and the DNA was extracted. After sequencing the SSU rDNA insert (1206 bp), we carried out a comparative sequence analysis with accessible SSU rRNA sequences from related eukaryotes. Escherichia coli and Methanospirilium hungatei were used as outgroups. Spironucleus muris shows strongest structural similarity (73.2%) to the parasitic flagellate Hexamita inflata (class Diplomonadida), which originated very early in the eukaryotic tree.

  • 16s like rdna sequence and phylogenetic position of the diplomonad Spironucleus muris lavier 1936
    European Journal of Protistology, 1996
    Co-Authors: Jurgen Branke, Manfred Berchtold, Alfred Breunig, Helmut König, Jörg Reimann
    Abstract:

    Summary The sequence of the small subunit (SSU) rDNA gene of the facultative pathogenic flagellate Spironucleus muris has been determined. This flagellate belongs to the class Diplomonadida. We will set out its phylogenetic position within the eukaryotic tree according to its morphological characteristics. Cells of Spironucleus muris were enriched from the digestive tract of immunodeficient mice and the DNA was extracted. After sequencing the SSU rDNA insert (1206 bp), we carried out a comparative sequence analysis with accessible SSU rRNA sequences from related eukaryotes. Escherichia coli and Methanospirilium hungatei were used as outgroups. Spironucleus muris shows strongest structural similarity (73.2%) to the parasitic flagellate Hexamita inflata (class Diplomonadida), which originated very early in the eukaryotic tree.

Alfred Breunig - One of the best experts on this subject based on the ideXlab platform.

  • 16s like rdna sequence and phylogenetic position of the diplomonad Spironucleus muris lavier 1936
    European Journal of Protistology, 1996
    Co-Authors: Jurgen Branke, Manfred Berchtold, Alfred Breunig, Helmut König, Jörg Reimann
    Abstract:

    Summary The sequence of the small subunit (SSU) rDNA gene of the facultative pathogenic flagellate Spironucleus muris has been determined. This flagellate belongs to the class Diplomonadida. We will set out its phylogenetic position within the eukaryotic tree according to its morphological characteristics. Cells of Spironucleus muris were enriched from the digestive tract of immunodeficient mice and the DNA was extracted. After sequencing the SSU rDNA insert (1206 bp), we carried out a comparative sequence analysis with accessible SSU rRNA sequences from related eukaryotes. Escherichia coli and Methanospirilium hungatei were used as outgroups. Spironucleus muris shows strongest structural similarity (73.2%) to the parasitic flagellate Hexamita inflata (class Diplomonadida), which originated very early in the eukaryotic tree.

  • 16s like rdna sequence and phylogenetic position of the diplomonad Spironucleus muris lavier 1936
    European Journal of Protistology, 1996
    Co-Authors: Jurgen Branke, Manfred Berchtold, Alfred Breunig, Helmut König, Jörg Reimann
    Abstract:

    Summary The sequence of the small subunit (SSU) rDNA gene of the facultative pathogenic flagellate Spironucleus muris has been determined. This flagellate belongs to the class Diplomonadida. We will set out its phylogenetic position within the eukaryotic tree according to its morphological characteristics. Cells of Spironucleus muris were enriched from the digestive tract of immunodeficient mice and the DNA was extracted. After sequencing the SSU rDNA insert (1206 bp), we carried out a comparative sequence analysis with accessible SSU rRNA sequences from related eukaryotes. Escherichia coli and Methanospirilium hungatei were used as outgroups. Spironucleus muris shows strongest structural similarity (73.2%) to the parasitic flagellate Hexamita inflata (class Diplomonadida), which originated very early in the eukaryotic tree.