The Experts below are selected from a list of 66687 Experts worldwide ranked by ideXlab platform
Eduardo Arzt - One of the best experts on this subject based on the ideXlab platform.
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trh receptor on immune cells in vitro and in vivo stimulation of human lymphocyte and rat splenocyte dna synthesis by trh
Journal of Clinical Immunology, 1995Co-Authors: Silvina Raiden, Edith Polack, V E Nahmod, Marta Labeur, F Holsboer, Eduardo ArztAbstract:This work examined whether (1) immune cells express thyrotrophin releasing hormone (TRH) receptor mRNA and (2) TRH modulates lymphocyte activation. By Northern blot of RNA extracted from human peripheral blood mononuclear cells (PBMC) and rat Splenocytes, a single TRH receptor mRNA band of about 3.8 kb (identical to that obtained from pituitary cells) was obtained, under both basal and stimulated conditions. A significant increase in DNA synthesis was observed in phytohemagglutinin-stimulated PBMC and concanavalin A (Con A) stimulated Splenocytes when TRH (10−6 M-10−12 M) was added. After 5, 30, 60, 180 min and 24 h of TRH administrationin vivo, a significant increase in the rat splenocyte proliferative response to Con A was observed.In vivo administration of anti-rat TSH antibody (1/1000) blocked the increase observed after 30 min of TRH administration on the Con A stimulated splenocyte response. TRH possess immunostimulatory functions directly via its receptor and indirectly via release of other immunostimulatory factors such as thyrotrophin.
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TRH receptor on immune cells:In vitro andin Vivo stimulation of human lymphocyte and rat splenocyte DNA synthesis by TRH
Journal of Clinical Immunology, 1995Co-Authors: Silvina Raiden, Edith Polack, V E Nahmod, Marta Labeur, F Holsboer, Eduardo ArztAbstract:This work examined whether (1) immune cells express thyrotrophin releasing hormone (TRH) receptor mRNA and (2) TRH modulates lymphocyte activation. By Northern blot of RNA extracted from human peripheral blood mononuclear cells (PBMC) and rat Splenocytes, a single TRH receptor mRNA band of about 3.8 kb (identical to that obtained from pituitary cells) was obtained, under both basal and stimulated conditions. A significant increase in DNA synthesis was observed in phytohemagglutinin-stimulated PBMC and concanavalin A (Con A) stimulated Splenocytes when TRH (10^−6 M-10^−12 M) was added. After 5, 30, 60, 180 min and 24 h of TRH administration in vivo , a significant increase in the rat splenocyte proliferative response to Con A was observed. In vivo administration of anti-rat TSH antibody (1/1000) blocked the increase observed after 30 min of TRH administration on the Con A stimulated splenocyte response. TRH possess immunostimulatory functions directly via its receptor and indirectly via release of other immunostimulatory factors such as thyrotrophin.
Silvina Raiden - One of the best experts on this subject based on the ideXlab platform.
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trh receptor on immune cells in vitro and in vivo stimulation of human lymphocyte and rat splenocyte dna synthesis by trh
Journal of Clinical Immunology, 1995Co-Authors: Silvina Raiden, Edith Polack, V E Nahmod, Marta Labeur, F Holsboer, Eduardo ArztAbstract:This work examined whether (1) immune cells express thyrotrophin releasing hormone (TRH) receptor mRNA and (2) TRH modulates lymphocyte activation. By Northern blot of RNA extracted from human peripheral blood mononuclear cells (PBMC) and rat Splenocytes, a single TRH receptor mRNA band of about 3.8 kb (identical to that obtained from pituitary cells) was obtained, under both basal and stimulated conditions. A significant increase in DNA synthesis was observed in phytohemagglutinin-stimulated PBMC and concanavalin A (Con A) stimulated Splenocytes when TRH (10−6 M-10−12 M) was added. After 5, 30, 60, 180 min and 24 h of TRH administrationin vivo, a significant increase in the rat splenocyte proliferative response to Con A was observed.In vivo administration of anti-rat TSH antibody (1/1000) blocked the increase observed after 30 min of TRH administration on the Con A stimulated splenocyte response. TRH possess immunostimulatory functions directly via its receptor and indirectly via release of other immunostimulatory factors such as thyrotrophin.
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TRH receptor on immune cells:In vitro andin Vivo stimulation of human lymphocyte and rat splenocyte DNA synthesis by TRH
Journal of Clinical Immunology, 1995Co-Authors: Silvina Raiden, Edith Polack, V E Nahmod, Marta Labeur, F Holsboer, Eduardo ArztAbstract:This work examined whether (1) immune cells express thyrotrophin releasing hormone (TRH) receptor mRNA and (2) TRH modulates lymphocyte activation. By Northern blot of RNA extracted from human peripheral blood mononuclear cells (PBMC) and rat Splenocytes, a single TRH receptor mRNA band of about 3.8 kb (identical to that obtained from pituitary cells) was obtained, under both basal and stimulated conditions. A significant increase in DNA synthesis was observed in phytohemagglutinin-stimulated PBMC and concanavalin A (Con A) stimulated Splenocytes when TRH (10^−6 M-10^−12 M) was added. After 5, 30, 60, 180 min and 24 h of TRH administration in vivo , a significant increase in the rat splenocyte proliferative response to Con A was observed. In vivo administration of anti-rat TSH antibody (1/1000) blocked the increase observed after 30 min of TRH administration on the Con A stimulated splenocyte response. TRH possess immunostimulatory functions directly via its receptor and indirectly via release of other immunostimulatory factors such as thyrotrophin.
Edith Polack - One of the best experts on this subject based on the ideXlab platform.
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trh receptor on immune cells in vitro and in vivo stimulation of human lymphocyte and rat splenocyte dna synthesis by trh
Journal of Clinical Immunology, 1995Co-Authors: Silvina Raiden, Edith Polack, V E Nahmod, Marta Labeur, F Holsboer, Eduardo ArztAbstract:This work examined whether (1) immune cells express thyrotrophin releasing hormone (TRH) receptor mRNA and (2) TRH modulates lymphocyte activation. By Northern blot of RNA extracted from human peripheral blood mononuclear cells (PBMC) and rat Splenocytes, a single TRH receptor mRNA band of about 3.8 kb (identical to that obtained from pituitary cells) was obtained, under both basal and stimulated conditions. A significant increase in DNA synthesis was observed in phytohemagglutinin-stimulated PBMC and concanavalin A (Con A) stimulated Splenocytes when TRH (10−6 M-10−12 M) was added. After 5, 30, 60, 180 min and 24 h of TRH administrationin vivo, a significant increase in the rat splenocyte proliferative response to Con A was observed.In vivo administration of anti-rat TSH antibody (1/1000) blocked the increase observed after 30 min of TRH administration on the Con A stimulated splenocyte response. TRH possess immunostimulatory functions directly via its receptor and indirectly via release of other immunostimulatory factors such as thyrotrophin.
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TRH receptor on immune cells:In vitro andin Vivo stimulation of human lymphocyte and rat splenocyte DNA synthesis by TRH
Journal of Clinical Immunology, 1995Co-Authors: Silvina Raiden, Edith Polack, V E Nahmod, Marta Labeur, F Holsboer, Eduardo ArztAbstract:This work examined whether (1) immune cells express thyrotrophin releasing hormone (TRH) receptor mRNA and (2) TRH modulates lymphocyte activation. By Northern blot of RNA extracted from human peripheral blood mononuclear cells (PBMC) and rat Splenocytes, a single TRH receptor mRNA band of about 3.8 kb (identical to that obtained from pituitary cells) was obtained, under both basal and stimulated conditions. A significant increase in DNA synthesis was observed in phytohemagglutinin-stimulated PBMC and concanavalin A (Con A) stimulated Splenocytes when TRH (10^−6 M-10^−12 M) was added. After 5, 30, 60, 180 min and 24 h of TRH administration in vivo , a significant increase in the rat splenocyte proliferative response to Con A was observed. In vivo administration of anti-rat TSH antibody (1/1000) blocked the increase observed after 30 min of TRH administration on the Con A stimulated splenocyte response. TRH possess immunostimulatory functions directly via its receptor and indirectly via release of other immunostimulatory factors such as thyrotrophin.
V E Nahmod - One of the best experts on this subject based on the ideXlab platform.
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trh receptor on immune cells in vitro and in vivo stimulation of human lymphocyte and rat splenocyte dna synthesis by trh
Journal of Clinical Immunology, 1995Co-Authors: Silvina Raiden, Edith Polack, V E Nahmod, Marta Labeur, F Holsboer, Eduardo ArztAbstract:This work examined whether (1) immune cells express thyrotrophin releasing hormone (TRH) receptor mRNA and (2) TRH modulates lymphocyte activation. By Northern blot of RNA extracted from human peripheral blood mononuclear cells (PBMC) and rat Splenocytes, a single TRH receptor mRNA band of about 3.8 kb (identical to that obtained from pituitary cells) was obtained, under both basal and stimulated conditions. A significant increase in DNA synthesis was observed in phytohemagglutinin-stimulated PBMC and concanavalin A (Con A) stimulated Splenocytes when TRH (10−6 M-10−12 M) was added. After 5, 30, 60, 180 min and 24 h of TRH administrationin vivo, a significant increase in the rat splenocyte proliferative response to Con A was observed.In vivo administration of anti-rat TSH antibody (1/1000) blocked the increase observed after 30 min of TRH administration on the Con A stimulated splenocyte response. TRH possess immunostimulatory functions directly via its receptor and indirectly via release of other immunostimulatory factors such as thyrotrophin.
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TRH receptor on immune cells:In vitro andin Vivo stimulation of human lymphocyte and rat splenocyte DNA synthesis by TRH
Journal of Clinical Immunology, 1995Co-Authors: Silvina Raiden, Edith Polack, V E Nahmod, Marta Labeur, F Holsboer, Eduardo ArztAbstract:This work examined whether (1) immune cells express thyrotrophin releasing hormone (TRH) receptor mRNA and (2) TRH modulates lymphocyte activation. By Northern blot of RNA extracted from human peripheral blood mononuclear cells (PBMC) and rat Splenocytes, a single TRH receptor mRNA band of about 3.8 kb (identical to that obtained from pituitary cells) was obtained, under both basal and stimulated conditions. A significant increase in DNA synthesis was observed in phytohemagglutinin-stimulated PBMC and concanavalin A (Con A) stimulated Splenocytes when TRH (10^−6 M-10^−12 M) was added. After 5, 30, 60, 180 min and 24 h of TRH administration in vivo , a significant increase in the rat splenocyte proliferative response to Con A was observed. In vivo administration of anti-rat TSH antibody (1/1000) blocked the increase observed after 30 min of TRH administration on the Con A stimulated splenocyte response. TRH possess immunostimulatory functions directly via its receptor and indirectly via release of other immunostimulatory factors such as thyrotrophin.
Marta Labeur - One of the best experts on this subject based on the ideXlab platform.
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trh receptor on immune cells in vitro and in vivo stimulation of human lymphocyte and rat splenocyte dna synthesis by trh
Journal of Clinical Immunology, 1995Co-Authors: Silvina Raiden, Edith Polack, V E Nahmod, Marta Labeur, F Holsboer, Eduardo ArztAbstract:This work examined whether (1) immune cells express thyrotrophin releasing hormone (TRH) receptor mRNA and (2) TRH modulates lymphocyte activation. By Northern blot of RNA extracted from human peripheral blood mononuclear cells (PBMC) and rat Splenocytes, a single TRH receptor mRNA band of about 3.8 kb (identical to that obtained from pituitary cells) was obtained, under both basal and stimulated conditions. A significant increase in DNA synthesis was observed in phytohemagglutinin-stimulated PBMC and concanavalin A (Con A) stimulated Splenocytes when TRH (10−6 M-10−12 M) was added. After 5, 30, 60, 180 min and 24 h of TRH administrationin vivo, a significant increase in the rat splenocyte proliferative response to Con A was observed.In vivo administration of anti-rat TSH antibody (1/1000) blocked the increase observed after 30 min of TRH administration on the Con A stimulated splenocyte response. TRH possess immunostimulatory functions directly via its receptor and indirectly via release of other immunostimulatory factors such as thyrotrophin.
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TRH receptor on immune cells:In vitro andin Vivo stimulation of human lymphocyte and rat splenocyte DNA synthesis by TRH
Journal of Clinical Immunology, 1995Co-Authors: Silvina Raiden, Edith Polack, V E Nahmod, Marta Labeur, F Holsboer, Eduardo ArztAbstract:This work examined whether (1) immune cells express thyrotrophin releasing hormone (TRH) receptor mRNA and (2) TRH modulates lymphocyte activation. By Northern blot of RNA extracted from human peripheral blood mononuclear cells (PBMC) and rat Splenocytes, a single TRH receptor mRNA band of about 3.8 kb (identical to that obtained from pituitary cells) was obtained, under both basal and stimulated conditions. A significant increase in DNA synthesis was observed in phytohemagglutinin-stimulated PBMC and concanavalin A (Con A) stimulated Splenocytes when TRH (10^−6 M-10^−12 M) was added. After 5, 30, 60, 180 min and 24 h of TRH administration in vivo , a significant increase in the rat splenocyte proliferative response to Con A was observed. In vivo administration of anti-rat TSH antibody (1/1000) blocked the increase observed after 30 min of TRH administration on the Con A stimulated splenocyte response. TRH possess immunostimulatory functions directly via its receptor and indirectly via release of other immunostimulatory factors such as thyrotrophin.
