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Javier Castrorosas - One of the best experts on this subject based on the ideXlab platform.
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presence and correlation of some enteric indicator bacteria diarrheagenic escherichia coli pathotypes and salmonella serotypes in alfalfa Sprouts from local retail markets in pachuca mexico
Journal of Food Protection, 2015Co-Authors: Esmeralda Rangelvargas, Carlos A Gomezaldapa, M Del Refugio Torresvitela, Angelica Villarruellopez, Alberto Jose Gordillomartinez, Javier CastrorosasAbstract:Data on the presence of diarrheagenic Escherichia coli pathotypes (DEPs) in alfalfa Sprouts and correlations between the presence of coliform bacteria (CB), fecal coliforms (FC), E. coli, DEPs, and Salmonella in alfalfa Sprouts are not available. The presence of and correlations between CB, FC, E. coli, DEPs, and Salmonella in alfalfa Sprouts were determined. One hundred Sprout samples were collected from retail markets in Pachuca, Hidalgo State, Mexico. The presence of indicator bacteria and Salmonella was determined using conventional culture procedures. DEPs were identified using two multiplex PCR procedures. One hundred percent of samples were positive for CB, 90% for FC, 84% for E. coli, 10% for DEPs, and 4% for Salmonella. The populations of CB ranged from 6.2 up to 8.6 log CFU/g. The FC and E. coli concentrations were between , 3 and 1,100 most probable number (MPN)/g. The DEPs identified included enterotoxigenic E. coli (ETEC; 2%), enteropathogenic E. coli (EPEC; 3%), and Shiga toxin-producing E. coli (STEC; 5%). No E. coli O157:H7 strains were detected in any STEC-positive samples. In samples positive for DEPs, the concentrations ranged from 210 to 240 MPN/g for ETEC, 28 to 1,100 MPN/g for EPEC, and 3.6 to 460 MPN/g for STEC. The Salmonella isolates identified included Salmonella enterica serotype Typhimurium in three samples and Salmonella enterica serotype Enteritidis in one. STEC and Salmonella Typhimurium were identified together in one sample. Positive correlations were observed between FC and E. coli, between FC and DEPs, and between E. coli and DEPs. Negative correlations occurred between CB and DEPs and between CB and Salmonella. Neither FC nor E. coli correlated with Salmonella in the Sprout samples. To our knowledge, this is the first report of ETEC, EPEC, and STEC isolated from alfalfa Sprouts and the first report of correlations between different indicator groups versus DEPs and Salmonella.
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presence of indicator bacteria salmonella and diarrheagenic escherichia coli pathotypes on mung bean Sprouts from public markets in pachuca mexico
Food Control, 2013Co-Authors: Jorge F Cernacortes, Carlos A Gomezaldapa, Esmeralda Rangelvargas, Enrique Ramirezcruz, Javier CastrorosasAbstract:Coliform bacteria (CB), fecal coliforms (FC), Escherichia coli, diarrheagenic E. coli pathotypes (DEP) and Salmonella frequencies were determined for mung bean (Vigna radiata) Sprouts. One hundred Sprout samples were collected from markets in Pachuca, Hidalgo state, Mexico. Of these samples, 100% were positive for CB, 98% for FC, 95% for E. coli, 10% for DEP and 5% for Salmonella. Identified DEP included enterotoxigenic E. coli (ETEC), enteroinvasive E. coli (EIEC) and Shiga toxin-producing E. coli (STEC). The ETEC and EIEC were each isolated from 2% of samples, and the STEC from 6% of samples. No E. coli O157:H7 were detected in any STEC-positive samples. Positive correlations were observed between FC and E. coli and between E. coli and DEP. A negative correlation occurred between CB and DEP, and between CB and Salmonella. Neither FC nor E. coli correlated with Salmonella presence in the Sprout samples. This is the first report of ETEC, EIEC and STEC isolated from Sprouts in Mexico and the first report of Salmonella isolation from mung bean in Mexico. Mung bean Sprouts are very probably an important factor contributing to the endemicity of ETEC, EIEC and STEC and Salmonella-related gastroenteritis in Mexico.
Hiroaki Yamauchi - One of the best experts on this subject based on the ideXlab platform.
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comparison of phenolic compositions between common and tartary buckwheat fagopyrum Sprouts
Food Chemistry, 2008Co-Authors: I.s.m. Zaidul, Sigenobu Takigawa, Chie Matsuuraendo, Tatsuro Suzuki, Yuji Mukasa, Takahiro Noda, Naoto Hashimoto, Hiroaki YamauchiAbstract:The phenolic compositions of non-germinated/germinated seeds and seed Sprouts (at 6–10 day-old) of common (Fagopyrum esculentum Moench) and tartary (Fagopyrum tataricum Gaertn.) buckwheats were investigated. Phenolic compounds, including chlorogenic acid, four C-glycosylflavones (orientin, isoorientin vitexin, isovitexin), rutin and quercetin, were determined in the seed Sprouts by high-performance liquid chromatography (HPLC). In the edible parts of common buckwheat Sprouts, individual phenolics significantly increased during Sprout growth from 6 to 10 days after sowing (DAS), whereas in tartary buckwheat Sprouts they did not. While the sum contents of phenolic compounds in the edible part (mean 24.4 mg/g DW at 6–10 DAS) of tartary buckwheat Sprouts were similar to those of common buckwheat Sprouts, rutin contents in the non-germinated/germinated seeds (mean 14.7 mg/g DW) and edible parts (mean 21.8 mg/g DW) of tartary buckwheat were 49- and 5-fold, respectively, higher than those of common buckwheat. Extracts of the edible parts of both species showed very similar free radical-scavenging activities (mean 1.7 μmol trolox eq/g DW), suggesting that the overall antioxidative activity might be affected by the combination of identified phenolics and unidentified (minor) components. Therefore, buckwheat seed Sprouts are recommended for their high antioxidative activity, as well as being an excellent dietary source of phenolic compounds, particularly tartary buckwheat Sprouts, being rich in rutin.
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comparison of phenolic compositions between common and tartary buckwheat fagopyrum Sprouts
Food Chemistry, 2008Co-Authors: I.s.m. Zaidul, Sigenobu Takigawa, Chie Matsuuraendo, Tatsuro Suzuki, Yuji Mukasa, Takahiro Noda, Naoto Hashimoto, Hiroaki YamauchiAbstract:The phenolic compositions of non-germinated/germinated seeds and seed Sprouts (at 6–10 day-old) of common (Fagopyrum esculentum Moench) and tartary (Fagopyrum tataricum Gaertn.) buckwheats were investigated. Phenolic compounds, including chlorogenic acid, four C-glycosylflavones (orientin, isoorientin vitexin, isovitexin), rutin and quercetin, were determined in the seed Sprouts by high-performance liquid chromatography (HPLC). In the edible parts of common buckwheat Sprouts, individual phenolics significantly increased during Sprout growth from 6 to 10 days after sowing (DAS), whereas in tartary buckwheat Sprouts they did not. While the sum contents of phenolic compounds in the edible part (mean 24.4 mg/g DW at 6–10 DAS) of tartary buckwheat Sprouts were similar to those of common buckwheat Sprouts, rutin contents in the non-germinated/germinated seeds (mean 14.7 mg/g DW) and edible parts (mean 21.8 mg/g DW) of tartary buckwheat were 49- and 5-fold, respectively, higher than those of common buckwheat. Extracts of the edible parts of both species showed very similar free radical-scavenging activities (mean 1.7 μmol trolox eq/g DW), suggesting that the overall antioxidative activity might be affected by the combination of identified phenolics and unidentified (minor) components. Therefore, buckwheat seed Sprouts are recommended for their high antioxidative activity, as well as being an excellent dietary source of phenolic compounds, particularly tartary buckwheat Sprouts, being rich in rutin.
Esmeralda Rangelvargas - One of the best experts on this subject based on the ideXlab platform.
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presence and correlation of some enteric indicator bacteria diarrheagenic escherichia coli pathotypes and salmonella serotypes in alfalfa Sprouts from local retail markets in pachuca mexico
Journal of Food Protection, 2015Co-Authors: Esmeralda Rangelvargas, Carlos A Gomezaldapa, M Del Refugio Torresvitela, Angelica Villarruellopez, Alberto Jose Gordillomartinez, Javier CastrorosasAbstract:Data on the presence of diarrheagenic Escherichia coli pathotypes (DEPs) in alfalfa Sprouts and correlations between the presence of coliform bacteria (CB), fecal coliforms (FC), E. coli, DEPs, and Salmonella in alfalfa Sprouts are not available. The presence of and correlations between CB, FC, E. coli, DEPs, and Salmonella in alfalfa Sprouts were determined. One hundred Sprout samples were collected from retail markets in Pachuca, Hidalgo State, Mexico. The presence of indicator bacteria and Salmonella was determined using conventional culture procedures. DEPs were identified using two multiplex PCR procedures. One hundred percent of samples were positive for CB, 90% for FC, 84% for E. coli, 10% for DEPs, and 4% for Salmonella. The populations of CB ranged from 6.2 up to 8.6 log CFU/g. The FC and E. coli concentrations were between , 3 and 1,100 most probable number (MPN)/g. The DEPs identified included enterotoxigenic E. coli (ETEC; 2%), enteropathogenic E. coli (EPEC; 3%), and Shiga toxin-producing E. coli (STEC; 5%). No E. coli O157:H7 strains were detected in any STEC-positive samples. In samples positive for DEPs, the concentrations ranged from 210 to 240 MPN/g for ETEC, 28 to 1,100 MPN/g for EPEC, and 3.6 to 460 MPN/g for STEC. The Salmonella isolates identified included Salmonella enterica serotype Typhimurium in three samples and Salmonella enterica serotype Enteritidis in one. STEC and Salmonella Typhimurium were identified together in one sample. Positive correlations were observed between FC and E. coli, between FC and DEPs, and between E. coli and DEPs. Negative correlations occurred between CB and DEPs and between CB and Salmonella. Neither FC nor E. coli correlated with Salmonella in the Sprout samples. To our knowledge, this is the first report of ETEC, EPEC, and STEC isolated from alfalfa Sprouts and the first report of correlations between different indicator groups versus DEPs and Salmonella.
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presence of indicator bacteria salmonella and diarrheagenic escherichia coli pathotypes on mung bean Sprouts from public markets in pachuca mexico
Food Control, 2013Co-Authors: Jorge F Cernacortes, Carlos A Gomezaldapa, Esmeralda Rangelvargas, Enrique Ramirezcruz, Javier CastrorosasAbstract:Coliform bacteria (CB), fecal coliforms (FC), Escherichia coli, diarrheagenic E. coli pathotypes (DEP) and Salmonella frequencies were determined for mung bean (Vigna radiata) Sprouts. One hundred Sprout samples were collected from markets in Pachuca, Hidalgo state, Mexico. Of these samples, 100% were positive for CB, 98% for FC, 95% for E. coli, 10% for DEP and 5% for Salmonella. Identified DEP included enterotoxigenic E. coli (ETEC), enteroinvasive E. coli (EIEC) and Shiga toxin-producing E. coli (STEC). The ETEC and EIEC were each isolated from 2% of samples, and the STEC from 6% of samples. No E. coli O157:H7 were detected in any STEC-positive samples. Positive correlations were observed between FC and E. coli and between E. coli and DEP. A negative correlation occurred between CB and DEP, and between CB and Salmonella. Neither FC nor E. coli correlated with Salmonella presence in the Sprout samples. This is the first report of ETEC, EIEC and STEC isolated from Sprouts in Mexico and the first report of Salmonella isolation from mung bean in Mexico. Mung bean Sprouts are very probably an important factor contributing to the endemicity of ETEC, EIEC and STEC and Salmonella-related gastroenteritis in Mexico.
Carlos A Gomezaldapa - One of the best experts on this subject based on the ideXlab platform.
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presence and correlation of some enteric indicator bacteria diarrheagenic escherichia coli pathotypes and salmonella serotypes in alfalfa Sprouts from local retail markets in pachuca mexico
Journal of Food Protection, 2015Co-Authors: Esmeralda Rangelvargas, Carlos A Gomezaldapa, M Del Refugio Torresvitela, Angelica Villarruellopez, Alberto Jose Gordillomartinez, Javier CastrorosasAbstract:Data on the presence of diarrheagenic Escherichia coli pathotypes (DEPs) in alfalfa Sprouts and correlations between the presence of coliform bacteria (CB), fecal coliforms (FC), E. coli, DEPs, and Salmonella in alfalfa Sprouts are not available. The presence of and correlations between CB, FC, E. coli, DEPs, and Salmonella in alfalfa Sprouts were determined. One hundred Sprout samples were collected from retail markets in Pachuca, Hidalgo State, Mexico. The presence of indicator bacteria and Salmonella was determined using conventional culture procedures. DEPs were identified using two multiplex PCR procedures. One hundred percent of samples were positive for CB, 90% for FC, 84% for E. coli, 10% for DEPs, and 4% for Salmonella. The populations of CB ranged from 6.2 up to 8.6 log CFU/g. The FC and E. coli concentrations were between , 3 and 1,100 most probable number (MPN)/g. The DEPs identified included enterotoxigenic E. coli (ETEC; 2%), enteropathogenic E. coli (EPEC; 3%), and Shiga toxin-producing E. coli (STEC; 5%). No E. coli O157:H7 strains were detected in any STEC-positive samples. In samples positive for DEPs, the concentrations ranged from 210 to 240 MPN/g for ETEC, 28 to 1,100 MPN/g for EPEC, and 3.6 to 460 MPN/g for STEC. The Salmonella isolates identified included Salmonella enterica serotype Typhimurium in three samples and Salmonella enterica serotype Enteritidis in one. STEC and Salmonella Typhimurium were identified together in one sample. Positive correlations were observed between FC and E. coli, between FC and DEPs, and between E. coli and DEPs. Negative correlations occurred between CB and DEPs and between CB and Salmonella. Neither FC nor E. coli correlated with Salmonella in the Sprout samples. To our knowledge, this is the first report of ETEC, EPEC, and STEC isolated from alfalfa Sprouts and the first report of correlations between different indicator groups versus DEPs and Salmonella.
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presence of indicator bacteria salmonella and diarrheagenic escherichia coli pathotypes on mung bean Sprouts from public markets in pachuca mexico
Food Control, 2013Co-Authors: Jorge F Cernacortes, Carlos A Gomezaldapa, Esmeralda Rangelvargas, Enrique Ramirezcruz, Javier CastrorosasAbstract:Coliform bacteria (CB), fecal coliforms (FC), Escherichia coli, diarrheagenic E. coli pathotypes (DEP) and Salmonella frequencies were determined for mung bean (Vigna radiata) Sprouts. One hundred Sprout samples were collected from markets in Pachuca, Hidalgo state, Mexico. Of these samples, 100% were positive for CB, 98% for FC, 95% for E. coli, 10% for DEP and 5% for Salmonella. Identified DEP included enterotoxigenic E. coli (ETEC), enteroinvasive E. coli (EIEC) and Shiga toxin-producing E. coli (STEC). The ETEC and EIEC were each isolated from 2% of samples, and the STEC from 6% of samples. No E. coli O157:H7 were detected in any STEC-positive samples. Positive correlations were observed between FC and E. coli and between E. coli and DEP. A negative correlation occurred between CB and DEP, and between CB and Salmonella. Neither FC nor E. coli correlated with Salmonella presence in the Sprout samples. This is the first report of ETEC, EIEC and STEC isolated from Sprouts in Mexico and the first report of Salmonella isolation from mung bean in Mexico. Mung bean Sprouts are very probably an important factor contributing to the endemicity of ETEC, EIEC and STEC and Salmonella-related gastroenteritis in Mexico.
I.s.m. Zaidul - One of the best experts on this subject based on the ideXlab platform.
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comparison of phenolic compositions between common and tartary buckwheat fagopyrum Sprouts
Food Chemistry, 2008Co-Authors: I.s.m. Zaidul, Sigenobu Takigawa, Chie Matsuuraendo, Tatsuro Suzuki, Yuji Mukasa, Takahiro Noda, Naoto Hashimoto, Hiroaki YamauchiAbstract:The phenolic compositions of non-germinated/germinated seeds and seed Sprouts (at 6–10 day-old) of common (Fagopyrum esculentum Moench) and tartary (Fagopyrum tataricum Gaertn.) buckwheats were investigated. Phenolic compounds, including chlorogenic acid, four C-glycosylflavones (orientin, isoorientin vitexin, isovitexin), rutin and quercetin, were determined in the seed Sprouts by high-performance liquid chromatography (HPLC). In the edible parts of common buckwheat Sprouts, individual phenolics significantly increased during Sprout growth from 6 to 10 days after sowing (DAS), whereas in tartary buckwheat Sprouts they did not. While the sum contents of phenolic compounds in the edible part (mean 24.4 mg/g DW at 6–10 DAS) of tartary buckwheat Sprouts were similar to those of common buckwheat Sprouts, rutin contents in the non-germinated/germinated seeds (mean 14.7 mg/g DW) and edible parts (mean 21.8 mg/g DW) of tartary buckwheat were 49- and 5-fold, respectively, higher than those of common buckwheat. Extracts of the edible parts of both species showed very similar free radical-scavenging activities (mean 1.7 μmol trolox eq/g DW), suggesting that the overall antioxidative activity might be affected by the combination of identified phenolics and unidentified (minor) components. Therefore, buckwheat seed Sprouts are recommended for their high antioxidative activity, as well as being an excellent dietary source of phenolic compounds, particularly tartary buckwheat Sprouts, being rich in rutin.
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comparison of phenolic compositions between common and tartary buckwheat fagopyrum Sprouts
Food Chemistry, 2008Co-Authors: I.s.m. Zaidul, Sigenobu Takigawa, Chie Matsuuraendo, Tatsuro Suzuki, Yuji Mukasa, Takahiro Noda, Naoto Hashimoto, Hiroaki YamauchiAbstract:The phenolic compositions of non-germinated/germinated seeds and seed Sprouts (at 6–10 day-old) of common (Fagopyrum esculentum Moench) and tartary (Fagopyrum tataricum Gaertn.) buckwheats were investigated. Phenolic compounds, including chlorogenic acid, four C-glycosylflavones (orientin, isoorientin vitexin, isovitexin), rutin and quercetin, were determined in the seed Sprouts by high-performance liquid chromatography (HPLC). In the edible parts of common buckwheat Sprouts, individual phenolics significantly increased during Sprout growth from 6 to 10 days after sowing (DAS), whereas in tartary buckwheat Sprouts they did not. While the sum contents of phenolic compounds in the edible part (mean 24.4 mg/g DW at 6–10 DAS) of tartary buckwheat Sprouts were similar to those of common buckwheat Sprouts, rutin contents in the non-germinated/germinated seeds (mean 14.7 mg/g DW) and edible parts (mean 21.8 mg/g DW) of tartary buckwheat were 49- and 5-fold, respectively, higher than those of common buckwheat. Extracts of the edible parts of both species showed very similar free radical-scavenging activities (mean 1.7 μmol trolox eq/g DW), suggesting that the overall antioxidative activity might be affected by the combination of identified phenolics and unidentified (minor) components. Therefore, buckwheat seed Sprouts are recommended for their high antioxidative activity, as well as being an excellent dietary source of phenolic compounds, particularly tartary buckwheat Sprouts, being rich in rutin.
