The Experts below are selected from a list of 8130 Experts worldwide ranked by ideXlab platform
Elisabeth Génot - One of the best experts on this subject based on the ideXlab platform.
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VEGF-A/Notch-Induced Podosomes Proteolyse Basement Membrane Collagen-IV during Retinal Sprouting Angiogenesis
Cell Reports, 2016Co-Authors: Pirjo Spuul, Isabelle Fremaux, Florian Alonso, Bettina Pitter, Eloi Montanez, Ïjsbrand M. Kramer, Thomas Daubon, Elisabeth GénotAbstract:During angiogenic Sprouting, endothelial tip cells emerge from existing vessels in a process that requires vascular basement membrane degradation. Here, we show that F-actin/cortactin/P-Src-based matrix-degrading microdomains called podosomes contribute to this step. In vitro, VEGF-A/Notch signaling regulates the formation of functional podosomes in endothelial cells. Using a retinal neovascularization model, we demonstrate that tip cells assemble podosomes during physiological Angiogenesis in vivo. In the retina, podosomes are also part of an interconnected network that surrounds large microvessels and impinges on the underlying basement membrane. Consistently, collagen-IV is scarce in podosome areas. Moreover, Notch inhibition exacerbates podosome formation and collagen-IV loss. We propose that the localized proteolytic action of podosomes on basement membrane collagen-IV facilitates endothelial cell Sprouting and anastomosis within the developing vasculature. The identification of podosomes as key components of the Sprouting machinery provides another opportunity to target Angiogenesis therapeutically.
Pirjo Spuul - One of the best experts on this subject based on the ideXlab platform.
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VEGF-A/Notch-Induced Podosomes Proteolyse Basement Membrane Collagen-IV during Retinal Sprouting Angiogenesis
Cell Reports, 2016Co-Authors: Pirjo Spuul, Isabelle Fremaux, Florian Alonso, Bettina Pitter, Eloi Montanez, Ïjsbrand M. Kramer, Thomas Daubon, Elisabeth GénotAbstract:During angiogenic Sprouting, endothelial tip cells emerge from existing vessels in a process that requires vascular basement membrane degradation. Here, we show that F-actin/cortactin/P-Src-based matrix-degrading microdomains called podosomes contribute to this step. In vitro, VEGF-A/Notch signaling regulates the formation of functional podosomes in endothelial cells. Using a retinal neovascularization model, we demonstrate that tip cells assemble podosomes during physiological Angiogenesis in vivo. In the retina, podosomes are also part of an interconnected network that surrounds large microvessels and impinges on the underlying basement membrane. Consistently, collagen-IV is scarce in podosome areas. Moreover, Notch inhibition exacerbates podosome formation and collagen-IV loss. We propose that the localized proteolytic action of podosomes on basement membrane collagen-IV facilitates endothelial cell Sprouting and anastomosis within the developing vasculature. The identification of podosomes as key components of the Sprouting machinery provides another opportunity to target Angiogenesis therapeutically.
Christopher C W Hughes - One of the best experts on this subject based on the ideXlab platform.
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3D Anastomosed Microvascular Network Model with Living Capillary Networks and Endothelial Cell-Lined Microfluidic Channels.
Methods of Molecular Biology, 2017Co-Authors: Xiaolin Wang, Duc T T Phan, Steven C George, Christopher C W HughesAbstract:This protocol describes detailed practical procedures for generating 3D intact and perfusable microvascular network that connects to microfluidic channels without appreciable leakage. This advanced 3D microvascular network model incorporates different stages of vascular development including vasculogenesis, endothelial cell (EC) lining, Sprouting Angiogenesis, and anastomosis in sequential order. The capillary network is first induced via vasculogenesis in a middle tissue chamber and then EC linings along the microfluidic channel on either side serve as artery and vein. The anastomosis is then induced by Sprouting Angiogenesis to facilitate tight interconnection between the artery/vein and the capillary network. This versatile device design and its robust construction methodology establish a physiological microcirculation transport model of interconnected perfused vessels from artery to vascularized tissue to vein.
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3d anastomosed microvascular network model with living capillary networks and endothelial cell lined microfluidic channels
Methods of Molecular Biology, 2017Co-Authors: Xiaolin Wang, Duc T T Phan, Steven C George, Christopher C W HughesAbstract:Author(s): Wang, Xiaolin; Phan, Duc TT; George, Steven C; Hughes, Christopher CW; Lee, Abraham P | Abstract: This protocol describes detailed practical procedures for generating 3D intact and perfusable microvascular network that connects to microfluidic channels without appreciable leakage. This advanced 3D microvascular network model incorporates different stages of vascular development including vasculogenesis, endothelial cell (EC) lining, Sprouting Angiogenesis, and anastomosis in sequential order. The capillary network is first induced via vasculogenesis in a middle tissue chamber and then EC linings along the microfluidic channel on either side serve as artery and vein. The anastomosis is then induced by Sprouting Angiogenesis to facilitate tight interconnection between the artery/vein and the capillary network. This versatile device design and its robust construction methodology establish a physiological microcirculation transport model of interconnected perfused vessels from artery to vascularized tissue to vein.
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engineering anastomosis between living capillary networks and endothelial cell lined microfluidic channels
Lab on a Chip, 2016Co-Authors: Xiaolin Wang, Duc T T Phan, Steven C George, Agua Sobrino, Christopher C W HughesAbstract:This paper reports a method for generating an intact and perfusable microvascular network that connects to microfluidic channels without appreciable leakage. This platform incorporates different stages of vascular development including vasculogenesis, endothelial cell (EC) lining, Sprouting Angiogenesis, and anastomosis in sequential order. After formation of a capillary network inside the tissue chamber via vasculogenesis, the adjacent microfluidic channels are lined with a monolayer of ECs, which then serve as the high-pressure input (“artery”) and low pressure output (“vein”) conduits. To promote a tight interconnection between the artery/vein and the capillary network, Sprouting Angiogenesis is induced, which promotes anastomosis of the vasculature inside the tissue chamber with the EC lining along the microfluidic channels. Flow of fluorescent microparticles confirms the perfusability of the lumenized microvascular network, and minimal leakage of 70 kDa FITC-dextran confirms physiologic tightness of the EC junctions and completeness of the interconnections between artery/vein and the capillary network. This versatile device design and its robust construction methodology establish a physiological transport model of interconnected perfused vessels from artery to vascularized tissue to vein. The system has utility in a wide range of organ-on-a-chip applications as it enables the physiological vascular interconnection of multiple on-chip tissue constructs that can serve as disease models for drug screening.
Yukiko T Matsunaga - One of the best experts on this subject based on the ideXlab platform.
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egfl7 regulates Sprouting Angiogenesis and endothelial integrity in a human blood vessel model
Biomaterials, 2019Co-Authors: Ryo Usuba, Joris Pauty, Fabrice Soncin, Yukiko T MatsunagaAbstract:Abstract Elucidating the mechanisms underlying Sprouting Angiogenesis and permeability should enable the development of more effective therapies for various diseases, including retinopathy, cancer, and other vascular disorders. We focused on epidermal growth factor like-domain 7 (EGFL7) which plays an important role in NOTCH signaling and in the organization of angiogenic sprouts. We developed an EGFL7 -knockdown in vitro microvessel model and investigated the effect of EGFL7 at a tissue level. We found EGFL7 knockdown suppressed VEGF-A-induced Sprouting Angiogenesis accompanied by an overproduction of endothelial filopodia and reduced collagen IV deposition at the basal side of endothelial cells. We also observed impaired barrier function which reflected an inflammatory condition. Furthermore, our results showed that proper formation of adherens junctions and phosphorylation of VE-cadherin was disturbed. In conclusion, by using a 3D microvessel model we identified novel roles for EGFL7 in endothelial function during Sprouting Angiogenesis.
Ïjsbrand M. Kramer - One of the best experts on this subject based on the ideXlab platform.
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VEGF-A/Notch-Induced Podosomes Proteolyse Basement Membrane Collagen-IV during Retinal Sprouting Angiogenesis
Cell Reports, 2016Co-Authors: Pirjo Spuul, Isabelle Fremaux, Florian Alonso, Bettina Pitter, Eloi Montanez, Ïjsbrand M. Kramer, Thomas Daubon, Elisabeth GénotAbstract:During angiogenic Sprouting, endothelial tip cells emerge from existing vessels in a process that requires vascular basement membrane degradation. Here, we show that F-actin/cortactin/P-Src-based matrix-degrading microdomains called podosomes contribute to this step. In vitro, VEGF-A/Notch signaling regulates the formation of functional podosomes in endothelial cells. Using a retinal neovascularization model, we demonstrate that tip cells assemble podosomes during physiological Angiogenesis in vivo. In the retina, podosomes are also part of an interconnected network that surrounds large microvessels and impinges on the underlying basement membrane. Consistently, collagen-IV is scarce in podosome areas. Moreover, Notch inhibition exacerbates podosome formation and collagen-IV loss. We propose that the localized proteolytic action of podosomes on basement membrane collagen-IV facilitates endothelial cell Sprouting and anastomosis within the developing vasculature. The identification of podosomes as key components of the Sprouting machinery provides another opportunity to target Angiogenesis therapeutically.