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Philippe Gautret - One of the best experts on this subject based on the ideXlab platform.

  • Sputum proteomic Analysis for distinguishing between pulmonary tuberculosis and non tuberculosis using matrix assisted laser desorption ionization time of flight mass spectrometry maldi tof ms preliminary results
    Clinical Microbiology and Infection, 2021
    Co-Authors: Thi Loi Dao, Herve Chaudet, Philippe Parola, Jean Christophe Lagier, Van Thuan Hoang, Sophie Alexandra Baron, Didier Raoult, Johan Courjon, Pierre Marty, Philippe Gautret
    Abstract:

    Abstract Objectives To evaluate the feasibility and diagnostic contribution of protein profiling using matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) applied to Sputum to diagnose pulmonary tuberculosis. Methods Sputum samples collected from patients suspected of having pulmonary tuberculosis were analysed using MALDI-TOF MS. Using the differentially expressed protein peaks, we compared three groups of patients, including those with confirmed pulmonary tuberculosis (PTB), those without tuberculosis but with a lower respiratory tract infection (non-TB LRTI), and those without tuberculosis and without an LRTI (non-TB controls). Results A total of 102 patients included 35 PTB, 36 non-TB LRTI and 31 non-TB controls. The model differentiated between the PTB patients and the non-TB controls using the 25 most differentially expressed protein peaks, with a sensitivity of 97%, 95%CI=[85%–100%] and a specificity of 77%, 95%CI=[59%-90%]. The model distinguished the PTB patients from the non-TB LRTI patients using the 10 most differentially expressed protein peaks, with a sensitivity of 80%, 95%CI=[63%–92%] and a specificity of 89%, 95%CI=[74%–97%]. We observed that the negative predictive value of MALDI-TOF MS Sputum Analysis was higher (96%, 95%CI=[80%–100%]) than that of direct Sputum microscopic examination and Sputum culture (78%, 95%CI=[62%–89%]) for non-TB controls. When MALDI-TOF MS Sputum Analysis and direct microscopic examination were combined, the negative predictive value reached 94%, 95%CI=[80%–99%] for non-TB LRTI patients. Conclusions These results suggest that MALDI-TOF MS Sputum Analysis coupled with microscopic examination could be used as a screening tool for diagnosing pulmonary TB.

  • Sputum proteomic Analysis for distinguishing between pulmonary tuberculosis and non tuberculosis using matrix assisted laser desorption ionization time of flight mass spectrometry maldi tof ms preliminary results
    Clinical Microbiology and Infection, 2021
    Co-Authors: Thi Loi Dao, Herve Chaudet, Philippe Parola, Jean Christophe Lagier, Van Thuan Hoang, Sophie Alexandra Baron, Didier Raoult, Johan Courjon, Pierre Marty, Philippe Gautret
    Abstract:

    Abstract Objectives The aim was to evaluate the feasibility and diagnostic contribution of protein profiling using matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) applied to Sputum to diagnose pulmonary tuberculosis. Methods Sputum samples collected from patients suspected of having pulmonary tuberculosis were analysed using MALDI-TOF MS. Using the differentially expressed protein peaks, we compared three groups of patients, including those with confirmed pulmonary tuberculosis (PTB), those without tuberculosis but with a lower respiratory tract infection (non-TB LRTI) and those without tuberculosis and without an LRTI (non-TB controls). Results A total of 102 patients included 35 PTB, 36 non-TB LRTI and 31 non-TB controls. The model differentiated between the PTB patients and the non-TB controls using the 25 most differentially expressed protein peaks, with a sensitivity of 97%, 95% CI 85–100%, and a specificity of 77%, 95% CI 59–90%. The model distinguished the PTB patients from the non-TB LRTI patients using the ten most differentially expressed protein peaks, with a sensitivity of 80%, 95% CI 63–92%, and a specificity of 89%, 95% CI 74–97%. We observed that the negative predictive value of MALDI-TOF MS Sputum Analysis was higher (96%, 95% CI 80–100%) than that of direct Sputum microscopic examination and Sputum culture (78%, 95% CI 62–89%) for non-TB controls. When MALDI-TOF MS Sputum Analysis and direct microscopic examination were combined, the negative predictive value reached 94%, 95% CI 80–99%, for non-TB LRTI patients. Discussion These results suggest that MALDI-TOF MS Sputum Analysis coupled with microscopic examination could be used as a screening tool for diagnosing pulmonary TB.

Thi Loi Dao - One of the best experts on this subject based on the ideXlab platform.

  • Sputum proteomic Analysis for distinguishing between pulmonary tuberculosis and non tuberculosis using matrix assisted laser desorption ionization time of flight mass spectrometry maldi tof ms preliminary results
    Clinical Microbiology and Infection, 2021
    Co-Authors: Thi Loi Dao, Herve Chaudet, Philippe Parola, Jean Christophe Lagier, Van Thuan Hoang, Sophie Alexandra Baron, Didier Raoult, Johan Courjon, Pierre Marty, Philippe Gautret
    Abstract:

    Abstract Objectives To evaluate the feasibility and diagnostic contribution of protein profiling using matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) applied to Sputum to diagnose pulmonary tuberculosis. Methods Sputum samples collected from patients suspected of having pulmonary tuberculosis were analysed using MALDI-TOF MS. Using the differentially expressed protein peaks, we compared three groups of patients, including those with confirmed pulmonary tuberculosis (PTB), those without tuberculosis but with a lower respiratory tract infection (non-TB LRTI), and those without tuberculosis and without an LRTI (non-TB controls). Results A total of 102 patients included 35 PTB, 36 non-TB LRTI and 31 non-TB controls. The model differentiated between the PTB patients and the non-TB controls using the 25 most differentially expressed protein peaks, with a sensitivity of 97%, 95%CI=[85%–100%] and a specificity of 77%, 95%CI=[59%-90%]. The model distinguished the PTB patients from the non-TB LRTI patients using the 10 most differentially expressed protein peaks, with a sensitivity of 80%, 95%CI=[63%–92%] and a specificity of 89%, 95%CI=[74%–97%]. We observed that the negative predictive value of MALDI-TOF MS Sputum Analysis was higher (96%, 95%CI=[80%–100%]) than that of direct Sputum microscopic examination and Sputum culture (78%, 95%CI=[62%–89%]) for non-TB controls. When MALDI-TOF MS Sputum Analysis and direct microscopic examination were combined, the negative predictive value reached 94%, 95%CI=[80%–99%] for non-TB LRTI patients. Conclusions These results suggest that MALDI-TOF MS Sputum Analysis coupled with microscopic examination could be used as a screening tool for diagnosing pulmonary TB.

  • Sputum proteomic Analysis for distinguishing between pulmonary tuberculosis and non tuberculosis using matrix assisted laser desorption ionization time of flight mass spectrometry maldi tof ms preliminary results
    Clinical Microbiology and Infection, 2021
    Co-Authors: Thi Loi Dao, Herve Chaudet, Philippe Parola, Jean Christophe Lagier, Van Thuan Hoang, Sophie Alexandra Baron, Didier Raoult, Johan Courjon, Pierre Marty, Philippe Gautret
    Abstract:

    Abstract Objectives The aim was to evaluate the feasibility and diagnostic contribution of protein profiling using matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) applied to Sputum to diagnose pulmonary tuberculosis. Methods Sputum samples collected from patients suspected of having pulmonary tuberculosis were analysed using MALDI-TOF MS. Using the differentially expressed protein peaks, we compared three groups of patients, including those with confirmed pulmonary tuberculosis (PTB), those without tuberculosis but with a lower respiratory tract infection (non-TB LRTI) and those without tuberculosis and without an LRTI (non-TB controls). Results A total of 102 patients included 35 PTB, 36 non-TB LRTI and 31 non-TB controls. The model differentiated between the PTB patients and the non-TB controls using the 25 most differentially expressed protein peaks, with a sensitivity of 97%, 95% CI 85–100%, and a specificity of 77%, 95% CI 59–90%. The model distinguished the PTB patients from the non-TB LRTI patients using the ten most differentially expressed protein peaks, with a sensitivity of 80%, 95% CI 63–92%, and a specificity of 89%, 95% CI 74–97%. We observed that the negative predictive value of MALDI-TOF MS Sputum Analysis was higher (96%, 95% CI 80–100%) than that of direct Sputum microscopic examination and Sputum culture (78%, 95% CI 62–89%) for non-TB controls. When MALDI-TOF MS Sputum Analysis and direct microscopic examination were combined, the negative predictive value reached 94%, 95% CI 80–99%, for non-TB LRTI patients. Discussion These results suggest that MALDI-TOF MS Sputum Analysis coupled with microscopic examination could be used as a screening tool for diagnosing pulmonary TB.

Van Thuan Hoang - One of the best experts on this subject based on the ideXlab platform.

  • Sputum proteomic Analysis for distinguishing between pulmonary tuberculosis and non tuberculosis using matrix assisted laser desorption ionization time of flight mass spectrometry maldi tof ms preliminary results
    Clinical Microbiology and Infection, 2021
    Co-Authors: Thi Loi Dao, Herve Chaudet, Philippe Parola, Jean Christophe Lagier, Van Thuan Hoang, Sophie Alexandra Baron, Didier Raoult, Johan Courjon, Pierre Marty, Philippe Gautret
    Abstract:

    Abstract Objectives To evaluate the feasibility and diagnostic contribution of protein profiling using matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) applied to Sputum to diagnose pulmonary tuberculosis. Methods Sputum samples collected from patients suspected of having pulmonary tuberculosis were analysed using MALDI-TOF MS. Using the differentially expressed protein peaks, we compared three groups of patients, including those with confirmed pulmonary tuberculosis (PTB), those without tuberculosis but with a lower respiratory tract infection (non-TB LRTI), and those without tuberculosis and without an LRTI (non-TB controls). Results A total of 102 patients included 35 PTB, 36 non-TB LRTI and 31 non-TB controls. The model differentiated between the PTB patients and the non-TB controls using the 25 most differentially expressed protein peaks, with a sensitivity of 97%, 95%CI=[85%–100%] and a specificity of 77%, 95%CI=[59%-90%]. The model distinguished the PTB patients from the non-TB LRTI patients using the 10 most differentially expressed protein peaks, with a sensitivity of 80%, 95%CI=[63%–92%] and a specificity of 89%, 95%CI=[74%–97%]. We observed that the negative predictive value of MALDI-TOF MS Sputum Analysis was higher (96%, 95%CI=[80%–100%]) than that of direct Sputum microscopic examination and Sputum culture (78%, 95%CI=[62%–89%]) for non-TB controls. When MALDI-TOF MS Sputum Analysis and direct microscopic examination were combined, the negative predictive value reached 94%, 95%CI=[80%–99%] for non-TB LRTI patients. Conclusions These results suggest that MALDI-TOF MS Sputum Analysis coupled with microscopic examination could be used as a screening tool for diagnosing pulmonary TB.

  • Sputum proteomic Analysis for distinguishing between pulmonary tuberculosis and non tuberculosis using matrix assisted laser desorption ionization time of flight mass spectrometry maldi tof ms preliminary results
    Clinical Microbiology and Infection, 2021
    Co-Authors: Thi Loi Dao, Herve Chaudet, Philippe Parola, Jean Christophe Lagier, Van Thuan Hoang, Sophie Alexandra Baron, Didier Raoult, Johan Courjon, Pierre Marty, Philippe Gautret
    Abstract:

    Abstract Objectives The aim was to evaluate the feasibility and diagnostic contribution of protein profiling using matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) applied to Sputum to diagnose pulmonary tuberculosis. Methods Sputum samples collected from patients suspected of having pulmonary tuberculosis were analysed using MALDI-TOF MS. Using the differentially expressed protein peaks, we compared three groups of patients, including those with confirmed pulmonary tuberculosis (PTB), those without tuberculosis but with a lower respiratory tract infection (non-TB LRTI) and those without tuberculosis and without an LRTI (non-TB controls). Results A total of 102 patients included 35 PTB, 36 non-TB LRTI and 31 non-TB controls. The model differentiated between the PTB patients and the non-TB controls using the 25 most differentially expressed protein peaks, with a sensitivity of 97%, 95% CI 85–100%, and a specificity of 77%, 95% CI 59–90%. The model distinguished the PTB patients from the non-TB LRTI patients using the ten most differentially expressed protein peaks, with a sensitivity of 80%, 95% CI 63–92%, and a specificity of 89%, 95% CI 74–97%. We observed that the negative predictive value of MALDI-TOF MS Sputum Analysis was higher (96%, 95% CI 80–100%) than that of direct Sputum microscopic examination and Sputum culture (78%, 95% CI 62–89%) for non-TB controls. When MALDI-TOF MS Sputum Analysis and direct microscopic examination were combined, the negative predictive value reached 94%, 95% CI 80–99%, for non-TB LRTI patients. Discussion These results suggest that MALDI-TOF MS Sputum Analysis coupled with microscopic examination could be used as a screening tool for diagnosing pulmonary TB.

Sophie Alexandra Baron - One of the best experts on this subject based on the ideXlab platform.

  • Sputum proteomic Analysis for distinguishing between pulmonary tuberculosis and non tuberculosis using matrix assisted laser desorption ionization time of flight mass spectrometry maldi tof ms preliminary results
    Clinical Microbiology and Infection, 2021
    Co-Authors: Thi Loi Dao, Herve Chaudet, Philippe Parola, Jean Christophe Lagier, Van Thuan Hoang, Sophie Alexandra Baron, Didier Raoult, Johan Courjon, Pierre Marty, Philippe Gautret
    Abstract:

    Abstract Objectives To evaluate the feasibility and diagnostic contribution of protein profiling using matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) applied to Sputum to diagnose pulmonary tuberculosis. Methods Sputum samples collected from patients suspected of having pulmonary tuberculosis were analysed using MALDI-TOF MS. Using the differentially expressed protein peaks, we compared three groups of patients, including those with confirmed pulmonary tuberculosis (PTB), those without tuberculosis but with a lower respiratory tract infection (non-TB LRTI), and those without tuberculosis and without an LRTI (non-TB controls). Results A total of 102 patients included 35 PTB, 36 non-TB LRTI and 31 non-TB controls. The model differentiated between the PTB patients and the non-TB controls using the 25 most differentially expressed protein peaks, with a sensitivity of 97%, 95%CI=[85%–100%] and a specificity of 77%, 95%CI=[59%-90%]. The model distinguished the PTB patients from the non-TB LRTI patients using the 10 most differentially expressed protein peaks, with a sensitivity of 80%, 95%CI=[63%–92%] and a specificity of 89%, 95%CI=[74%–97%]. We observed that the negative predictive value of MALDI-TOF MS Sputum Analysis was higher (96%, 95%CI=[80%–100%]) than that of direct Sputum microscopic examination and Sputum culture (78%, 95%CI=[62%–89%]) for non-TB controls. When MALDI-TOF MS Sputum Analysis and direct microscopic examination were combined, the negative predictive value reached 94%, 95%CI=[80%–99%] for non-TB LRTI patients. Conclusions These results suggest that MALDI-TOF MS Sputum Analysis coupled with microscopic examination could be used as a screening tool for diagnosing pulmonary TB.

  • Sputum proteomic Analysis for distinguishing between pulmonary tuberculosis and non tuberculosis using matrix assisted laser desorption ionization time of flight mass spectrometry maldi tof ms preliminary results
    Clinical Microbiology and Infection, 2021
    Co-Authors: Thi Loi Dao, Herve Chaudet, Philippe Parola, Jean Christophe Lagier, Van Thuan Hoang, Sophie Alexandra Baron, Didier Raoult, Johan Courjon, Pierre Marty, Philippe Gautret
    Abstract:

    Abstract Objectives The aim was to evaluate the feasibility and diagnostic contribution of protein profiling using matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) applied to Sputum to diagnose pulmonary tuberculosis. Methods Sputum samples collected from patients suspected of having pulmonary tuberculosis were analysed using MALDI-TOF MS. Using the differentially expressed protein peaks, we compared three groups of patients, including those with confirmed pulmonary tuberculosis (PTB), those without tuberculosis but with a lower respiratory tract infection (non-TB LRTI) and those without tuberculosis and without an LRTI (non-TB controls). Results A total of 102 patients included 35 PTB, 36 non-TB LRTI and 31 non-TB controls. The model differentiated between the PTB patients and the non-TB controls using the 25 most differentially expressed protein peaks, with a sensitivity of 97%, 95% CI 85–100%, and a specificity of 77%, 95% CI 59–90%. The model distinguished the PTB patients from the non-TB LRTI patients using the ten most differentially expressed protein peaks, with a sensitivity of 80%, 95% CI 63–92%, and a specificity of 89%, 95% CI 74–97%. We observed that the negative predictive value of MALDI-TOF MS Sputum Analysis was higher (96%, 95% CI 80–100%) than that of direct Sputum microscopic examination and Sputum culture (78%, 95% CI 62–89%) for non-TB controls. When MALDI-TOF MS Sputum Analysis and direct microscopic examination were combined, the negative predictive value reached 94%, 95% CI 80–99%, for non-TB LRTI patients. Discussion These results suggest that MALDI-TOF MS Sputum Analysis coupled with microscopic examination could be used as a screening tool for diagnosing pulmonary TB.

Pierre Marty - One of the best experts on this subject based on the ideXlab platform.

  • Sputum proteomic Analysis for distinguishing between pulmonary tuberculosis and non tuberculosis using matrix assisted laser desorption ionization time of flight mass spectrometry maldi tof ms preliminary results
    Clinical Microbiology and Infection, 2021
    Co-Authors: Thi Loi Dao, Herve Chaudet, Philippe Parola, Jean Christophe Lagier, Van Thuan Hoang, Sophie Alexandra Baron, Didier Raoult, Johan Courjon, Pierre Marty, Philippe Gautret
    Abstract:

    Abstract Objectives To evaluate the feasibility and diagnostic contribution of protein profiling using matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) applied to Sputum to diagnose pulmonary tuberculosis. Methods Sputum samples collected from patients suspected of having pulmonary tuberculosis were analysed using MALDI-TOF MS. Using the differentially expressed protein peaks, we compared three groups of patients, including those with confirmed pulmonary tuberculosis (PTB), those without tuberculosis but with a lower respiratory tract infection (non-TB LRTI), and those without tuberculosis and without an LRTI (non-TB controls). Results A total of 102 patients included 35 PTB, 36 non-TB LRTI and 31 non-TB controls. The model differentiated between the PTB patients and the non-TB controls using the 25 most differentially expressed protein peaks, with a sensitivity of 97%, 95%CI=[85%–100%] and a specificity of 77%, 95%CI=[59%-90%]. The model distinguished the PTB patients from the non-TB LRTI patients using the 10 most differentially expressed protein peaks, with a sensitivity of 80%, 95%CI=[63%–92%] and a specificity of 89%, 95%CI=[74%–97%]. We observed that the negative predictive value of MALDI-TOF MS Sputum Analysis was higher (96%, 95%CI=[80%–100%]) than that of direct Sputum microscopic examination and Sputum culture (78%, 95%CI=[62%–89%]) for non-TB controls. When MALDI-TOF MS Sputum Analysis and direct microscopic examination were combined, the negative predictive value reached 94%, 95%CI=[80%–99%] for non-TB LRTI patients. Conclusions These results suggest that MALDI-TOF MS Sputum Analysis coupled with microscopic examination could be used as a screening tool for diagnosing pulmonary TB.

  • Sputum proteomic Analysis for distinguishing between pulmonary tuberculosis and non tuberculosis using matrix assisted laser desorption ionization time of flight mass spectrometry maldi tof ms preliminary results
    Clinical Microbiology and Infection, 2021
    Co-Authors: Thi Loi Dao, Herve Chaudet, Philippe Parola, Jean Christophe Lagier, Van Thuan Hoang, Sophie Alexandra Baron, Didier Raoult, Johan Courjon, Pierre Marty, Philippe Gautret
    Abstract:

    Abstract Objectives The aim was to evaluate the feasibility and diagnostic contribution of protein profiling using matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) applied to Sputum to diagnose pulmonary tuberculosis. Methods Sputum samples collected from patients suspected of having pulmonary tuberculosis were analysed using MALDI-TOF MS. Using the differentially expressed protein peaks, we compared three groups of patients, including those with confirmed pulmonary tuberculosis (PTB), those without tuberculosis but with a lower respiratory tract infection (non-TB LRTI) and those without tuberculosis and without an LRTI (non-TB controls). Results A total of 102 patients included 35 PTB, 36 non-TB LRTI and 31 non-TB controls. The model differentiated between the PTB patients and the non-TB controls using the 25 most differentially expressed protein peaks, with a sensitivity of 97%, 95% CI 85–100%, and a specificity of 77%, 95% CI 59–90%. The model distinguished the PTB patients from the non-TB LRTI patients using the ten most differentially expressed protein peaks, with a sensitivity of 80%, 95% CI 63–92%, and a specificity of 89%, 95% CI 74–97%. We observed that the negative predictive value of MALDI-TOF MS Sputum Analysis was higher (96%, 95% CI 80–100%) than that of direct Sputum microscopic examination and Sputum culture (78%, 95% CI 62–89%) for non-TB controls. When MALDI-TOF MS Sputum Analysis and direct microscopic examination were combined, the negative predictive value reached 94%, 95% CI 80–99%, for non-TB LRTI patients. Discussion These results suggest that MALDI-TOF MS Sputum Analysis coupled with microscopic examination could be used as a screening tool for diagnosing pulmonary TB.