The Experts below are selected from a list of 219 Experts worldwide ranked by ideXlab platform

Werner W Franke - One of the best experts on this subject based on the ideXlab platform.

  • The different structures containing tight junction proteins in epidermal and other stratified epithelial Cells, including Squamous Cell Metaplasia.
    European journal of cell biology, 2007
    Co-Authors: Holger Schlüter, Hartwig Wolburg, Ingrid Moll, Werner W Franke
    Abstract:

    In stratified Squamous epithelia constituent proteins of tight junctions (TJs) are not restricted to the zonula occludens-related structures of the uppermost living Cell layer such as the stratum granulosum of the epidermis but TJ membrane proteins such as occludin and certain members of the claudin family as well as TJ plaque proteins, notably cingulin and protein ZO-1, have also been identified by immunofluorescence and immunoelectron microscopy in more basal layers where they form special Cell-Cell-connecting structures such as the "lamellated" and the "sandwich" junctions. In the present study, we describe another TJ protein-containing structure, the very small puncta occludentia ("stud junctions"), as the smallest identifiable TJ-like unit that occurs in most, perhaps all strata. We have also determined the specific distributions of TJ proteins in the Cell layers of Squamous Cell Metaplasias of the human bronchial tract. Moreover, we show that the occludin-related tetraspanin protein triCellulin-alpha connects and seals the membranes of adjacent "three corner" Cell structures of the uppermost layer in keratinocytes growing in culture. We hypothesize the possible occurrence of triCellulin-beta in more basal Cell layers of keratinocyte cultures and the general occurrence of different triCellulin splice forms in stratified epithelia in situ, and discuss the possible functions of TJ proteins in stratified epithelia and tumors derived therefrom.

  • The different structures containing tight junction proteins in epidermal and other stratified epithelial Cells, including Squamous Cell Metaplasia
    European Journal of Cell Biology, 2007
    Co-Authors: Holger Schlüter, Hartwig Wolburg, Ingrid Moll, Werner W Franke
    Abstract:

    In stratified Squamous epithelia constituent proteins of tight junctions (TJs) are not restricted to the zonula occludens-related structures of the uppermost living Cell layer such as the stratum granulosum of the epidermis but TJ membrane proteins such as occludin and certain members of the claudin family as well as TJ plaque proteins, notably cingulin and protein ZO-1, have also been identified by immunofluorescence and immunoelectron microscopy in more basal layers where they form special Cell–Cell-connecting structures such as the “lamellated” and the “sandwich” junctions. In the present study, we describe another TJ protein-containing structure, the very small puncta occludentia (“stud junctions”), as the smallest identifiable TJ-like unit that occurs in most, perhaps all strata. We have also determined the specific distributions of TJ proteins in the Cell layers of Squamous Cell Metaplasias of the human bronchial tract. Moreover, we show that the occludin-related tetraspanin protein triCellulin-α connects and seals the membranes of adjacent “three corner” Cell structures of the uppermost layer in keratinocytes growing in culture. We hypothesize the possible occurrence of triCellulin-β in more basal Cell layers of keratinocyte cultures and the general occurrence of different triCellulin splice forms in stratified epithelia in situ, and discuss the possible functions of TJ proteins in stratified epithelia and tumors derived therefrom.

Pieter S. Hiemstra - One of the best experts on this subject based on the ideXlab platform.

  • Smoking cessation and bronchial epithelial remodelling in COPD: a cross-sectional study
    Respiratory research, 2007
    Co-Authors: Thérèse S. Lapperre, Jacob K. Sont, Annemarie Van Schadewijk, M. M. E. Gosman, Dirkje S. Postma, Ingeborg M. Bajema, Wim Timens, Thais Mauad, Pieter S. Hiemstra
    Abstract:

    Background: Chronic Obstructive Pulmonary Disease (COPD) is associated with bronchial epithelial changes, including Squamous Cell Metaplasia and goblet Cell hyperplasia. These features are partially attributed to activation of the epidermal growth factor receptor (EGFR). Whereas smoking cessation reduces respiratory symptoms and lung function decline in COPD, inflammation persists. We determined epithelial proliferation and composition in bronchial biopsies from current and ex-smokers with COPD, and its relation to duration of smoking cessation. Methods: 114 COPD patients were studied cross-sectionally: 99 males/15 females, age 62 ± 8 years, median 42 pack-years, no corticosteroids, current (n = 72) or ex-smokers (n = 42, median cessation duration 3.5 years), postbronchodilator FEV 1 63 ± 9% predicted. Squamous Cell Metaplasia (%), goblet Cell (PAS/Alcian Blue+) area (%), proliferating (Ki-67 + ) Cell numbers (/mm basement membrane), and EGFR expression (%) were measured in intact epithelium of bronchial biopsies. Results: Ex-smokers with COPD had significantly less epithelial Squamous Cell Metaplasia, proliferating Cell numbers, and a trend towards reduced goblet Cell area than current smokers with COPD (p = 0.025, p = 0.001, p = 0.081, respectively), but no significant difference in EGFR expression. Epithelial features were not different between short-term quitters ( 3.5 years). Trial registration: NCT00158847

  • Human neutrophil defensins and secretory leukocyte proteinase inhibitor in Squamous metaplastic epithelium of bronchial airways.
    Inflammation research : official journal of the European Histamine Research Society ... [et al.], 2004
    Co-Authors: Jamil Aarbiou, Jacob K. Sont, Thais Mauad, A. Van Schadewijk, Jan Stolk, W. I. De Boer, Klaus F. Rabe, J.h.j.m. Van Krieken, Pieter S. Hiemstra
    Abstract:

    Objective:The aim of this study was to analyze a possible contribution of human neutrophil defensins and secretory leukocyte proteinase inhibitor (SLPI) to the induction of airway epithelial changes such as Squamous Cell Metaplasia.

Holger Schlüter - One of the best experts on this subject based on the ideXlab platform.

  • The different structures containing tight junction proteins in epidermal and other stratified epithelial Cells, including Squamous Cell Metaplasia.
    European journal of cell biology, 2007
    Co-Authors: Holger Schlüter, Hartwig Wolburg, Ingrid Moll, Werner W Franke
    Abstract:

    In stratified Squamous epithelia constituent proteins of tight junctions (TJs) are not restricted to the zonula occludens-related structures of the uppermost living Cell layer such as the stratum granulosum of the epidermis but TJ membrane proteins such as occludin and certain members of the claudin family as well as TJ plaque proteins, notably cingulin and protein ZO-1, have also been identified by immunofluorescence and immunoelectron microscopy in more basal layers where they form special Cell-Cell-connecting structures such as the "lamellated" and the "sandwich" junctions. In the present study, we describe another TJ protein-containing structure, the very small puncta occludentia ("stud junctions"), as the smallest identifiable TJ-like unit that occurs in most, perhaps all strata. We have also determined the specific distributions of TJ proteins in the Cell layers of Squamous Cell Metaplasias of the human bronchial tract. Moreover, we show that the occludin-related tetraspanin protein triCellulin-alpha connects and seals the membranes of adjacent "three corner" Cell structures of the uppermost layer in keratinocytes growing in culture. We hypothesize the possible occurrence of triCellulin-beta in more basal Cell layers of keratinocyte cultures and the general occurrence of different triCellulin splice forms in stratified epithelia in situ, and discuss the possible functions of TJ proteins in stratified epithelia and tumors derived therefrom.

  • The different structures containing tight junction proteins in epidermal and other stratified epithelial Cells, including Squamous Cell Metaplasia
    European Journal of Cell Biology, 2007
    Co-Authors: Holger Schlüter, Hartwig Wolburg, Ingrid Moll, Werner W Franke
    Abstract:

    In stratified Squamous epithelia constituent proteins of tight junctions (TJs) are not restricted to the zonula occludens-related structures of the uppermost living Cell layer such as the stratum granulosum of the epidermis but TJ membrane proteins such as occludin and certain members of the claudin family as well as TJ plaque proteins, notably cingulin and protein ZO-1, have also been identified by immunofluorescence and immunoelectron microscopy in more basal layers where they form special Cell–Cell-connecting structures such as the “lamellated” and the “sandwich” junctions. In the present study, we describe another TJ protein-containing structure, the very small puncta occludentia (“stud junctions”), as the smallest identifiable TJ-like unit that occurs in most, perhaps all strata. We have also determined the specific distributions of TJ proteins in the Cell layers of Squamous Cell Metaplasias of the human bronchial tract. Moreover, we show that the occludin-related tetraspanin protein triCellulin-α connects and seals the membranes of adjacent “three corner” Cell structures of the uppermost layer in keratinocytes growing in culture. We hypothesize the possible occurrence of triCellulin-β in more basal Cell layers of keratinocyte cultures and the general occurrence of different triCellulin splice forms in stratified epithelia in situ, and discuss the possible functions of TJ proteins in stratified epithelia and tumors derived therefrom.

Ingrid Moll - One of the best experts on this subject based on the ideXlab platform.

  • The different structures containing tight junction proteins in epidermal and other stratified epithelial Cells, including Squamous Cell Metaplasia.
    European journal of cell biology, 2007
    Co-Authors: Holger Schlüter, Hartwig Wolburg, Ingrid Moll, Werner W Franke
    Abstract:

    In stratified Squamous epithelia constituent proteins of tight junctions (TJs) are not restricted to the zonula occludens-related structures of the uppermost living Cell layer such as the stratum granulosum of the epidermis but TJ membrane proteins such as occludin and certain members of the claudin family as well as TJ plaque proteins, notably cingulin and protein ZO-1, have also been identified by immunofluorescence and immunoelectron microscopy in more basal layers where they form special Cell-Cell-connecting structures such as the "lamellated" and the "sandwich" junctions. In the present study, we describe another TJ protein-containing structure, the very small puncta occludentia ("stud junctions"), as the smallest identifiable TJ-like unit that occurs in most, perhaps all strata. We have also determined the specific distributions of TJ proteins in the Cell layers of Squamous Cell Metaplasias of the human bronchial tract. Moreover, we show that the occludin-related tetraspanin protein triCellulin-alpha connects and seals the membranes of adjacent "three corner" Cell structures of the uppermost layer in keratinocytes growing in culture. We hypothesize the possible occurrence of triCellulin-beta in more basal Cell layers of keratinocyte cultures and the general occurrence of different triCellulin splice forms in stratified epithelia in situ, and discuss the possible functions of TJ proteins in stratified epithelia and tumors derived therefrom.

  • The different structures containing tight junction proteins in epidermal and other stratified epithelial Cells, including Squamous Cell Metaplasia
    European Journal of Cell Biology, 2007
    Co-Authors: Holger Schlüter, Hartwig Wolburg, Ingrid Moll, Werner W Franke
    Abstract:

    In stratified Squamous epithelia constituent proteins of tight junctions (TJs) are not restricted to the zonula occludens-related structures of the uppermost living Cell layer such as the stratum granulosum of the epidermis but TJ membrane proteins such as occludin and certain members of the claudin family as well as TJ plaque proteins, notably cingulin and protein ZO-1, have also been identified by immunofluorescence and immunoelectron microscopy in more basal layers where they form special Cell–Cell-connecting structures such as the “lamellated” and the “sandwich” junctions. In the present study, we describe another TJ protein-containing structure, the very small puncta occludentia (“stud junctions”), as the smallest identifiable TJ-like unit that occurs in most, perhaps all strata. We have also determined the specific distributions of TJ proteins in the Cell layers of Squamous Cell Metaplasias of the human bronchial tract. Moreover, we show that the occludin-related tetraspanin protein triCellulin-α connects and seals the membranes of adjacent “three corner” Cell structures of the uppermost layer in keratinocytes growing in culture. We hypothesize the possible occurrence of triCellulin-β in more basal Cell layers of keratinocyte cultures and the general occurrence of different triCellulin splice forms in stratified epithelia in situ, and discuss the possible functions of TJ proteins in stratified epithelia and tumors derived therefrom.

Hartwig Wolburg - One of the best experts on this subject based on the ideXlab platform.

  • The different structures containing tight junction proteins in epidermal and other stratified epithelial Cells, including Squamous Cell Metaplasia.
    European journal of cell biology, 2007
    Co-Authors: Holger Schlüter, Hartwig Wolburg, Ingrid Moll, Werner W Franke
    Abstract:

    In stratified Squamous epithelia constituent proteins of tight junctions (TJs) are not restricted to the zonula occludens-related structures of the uppermost living Cell layer such as the stratum granulosum of the epidermis but TJ membrane proteins such as occludin and certain members of the claudin family as well as TJ plaque proteins, notably cingulin and protein ZO-1, have also been identified by immunofluorescence and immunoelectron microscopy in more basal layers where they form special Cell-Cell-connecting structures such as the "lamellated" and the "sandwich" junctions. In the present study, we describe another TJ protein-containing structure, the very small puncta occludentia ("stud junctions"), as the smallest identifiable TJ-like unit that occurs in most, perhaps all strata. We have also determined the specific distributions of TJ proteins in the Cell layers of Squamous Cell Metaplasias of the human bronchial tract. Moreover, we show that the occludin-related tetraspanin protein triCellulin-alpha connects and seals the membranes of adjacent "three corner" Cell structures of the uppermost layer in keratinocytes growing in culture. We hypothesize the possible occurrence of triCellulin-beta in more basal Cell layers of keratinocyte cultures and the general occurrence of different triCellulin splice forms in stratified epithelia in situ, and discuss the possible functions of TJ proteins in stratified epithelia and tumors derived therefrom.

  • The different structures containing tight junction proteins in epidermal and other stratified epithelial Cells, including Squamous Cell Metaplasia
    European Journal of Cell Biology, 2007
    Co-Authors: Holger Schlüter, Hartwig Wolburg, Ingrid Moll, Werner W Franke
    Abstract:

    In stratified Squamous epithelia constituent proteins of tight junctions (TJs) are not restricted to the zonula occludens-related structures of the uppermost living Cell layer such as the stratum granulosum of the epidermis but TJ membrane proteins such as occludin and certain members of the claudin family as well as TJ plaque proteins, notably cingulin and protein ZO-1, have also been identified by immunofluorescence and immunoelectron microscopy in more basal layers where they form special Cell–Cell-connecting structures such as the “lamellated” and the “sandwich” junctions. In the present study, we describe another TJ protein-containing structure, the very small puncta occludentia (“stud junctions”), as the smallest identifiable TJ-like unit that occurs in most, perhaps all strata. We have also determined the specific distributions of TJ proteins in the Cell layers of Squamous Cell Metaplasias of the human bronchial tract. Moreover, we show that the occludin-related tetraspanin protein triCellulin-α connects and seals the membranes of adjacent “three corner” Cell structures of the uppermost layer in keratinocytes growing in culture. We hypothesize the possible occurrence of triCellulin-β in more basal Cell layers of keratinocyte cultures and the general occurrence of different triCellulin splice forms in stratified epithelia in situ, and discuss the possible functions of TJ proteins in stratified epithelia and tumors derived therefrom.