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Jun Shimazaki - One of the best experts on this subject based on the ideXlab platform.
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oxidative stress induced age dependent meibomian gland dysfunction in cu zn superoxide dismutase 1 sod1 knockout mice
PLOS ONE, 2014Co-Authors: Osama M A Ibrahim, Takahiko Shimizu, Murat Dogru, Jun Shimazaki, Takashi Kojima, Yukihiro Matsumoto, Ayako Igarashi, Tais Hitomi Wakamatsu, Takaaki Inaba, Kazuo TsubotaAbstract:Purpose The purpose of our study was to investigate alterations in the meibomian gland (MG) in Cu, Zn-Superoxide Dismutase-1 knockout (Sod1−/−) mouse. Methods Tear function tests [Break up time (BUT) and cotton thread] and ocular vital Staining test were performed on Sod1−/− male mice (n = 24) aged 10 and 50 weeks, and age and sex matched wild–type (+/+) mice (n = 25). Tear and serum samples were collected at sacrifice for inflammatory cytokine assays. MG specimens underwent Hematoxylin and Eosin Staining, Mallory Staining for fibrosis, Oil Red O lipid Staining, TUNEL Staining, immunohistochemistry Stainings for 4HNE, 8-OHdG and CD45. Transmission electron microscopic examination (TEM) was also performed. Results Corneal vital Staining scores in the Sod1−/− mice were significantly higher compared with the wild type mice throughout the follow-up. Tear and serum IL-6 and TNF-α levels also showed significant elevations in the 10 to 50 week Sod1−/− mice. Oil Red O Staining showed an accumulation of large lipid droplets in the Sod1−/− mice at 50 weeks. Immunohistochemistry revealed both increased TUNEL and oxidative stress marker Stainings of the MG acinar epithelium in the Sod1−/− mice compared to the wild type mice. Immunohistochemistry Staining for CD45 showed increasing inflammatory cell infiltrates from 10 to 50 weeks in the Sod1−/− mice compared to the wild type mice. TEM revealed prominent mitochondrial changes in 50 week Sod1−/− mice. Conclusions Our results suggest that reactive oxygen species might play a vital role in the pathogensis of meibomian gland dysfunction. The Sod1−/− mouse appears to be a promising model for the study of reactive oxygen species associated MG alterations.
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the effects of 3 diquafosol sodium application on the tear functions and ocular surface of the cu zn superoxide dismutase 1 sod1 knockout mice
Molecular Vision, 2014Co-Authors: Takashi Kojima, Taeko Nagata, Seika Shimazaki, Osama M A Ibrahim, Kazunari Higa, Yoshiyuki Satake, Takuji Shirasawa, Takahiko Shimizu, Murat Dogru, Jun ShimazakiAbstract:−/− −/− male mice with C57BL/background and 14 eyes of 7 C57BL6 strain wild-type male mice were examined at 40 weeks in this study. All mice had application of 3% diquafosol ophthalmic solution six times a day for 2 weeks. Tear film stability and corneal epithelial damage was evaluated by fluorescein and Rose Bengal Stainings. Anterior segment photography was performed before and after eye drop instillations. Aqueous tear quantity was measured with phenol red–impregnated cotton threads without anesthesia. Animals were sacrificed at 42 weeks after diquafosol treatment and the whole globe specimens were subjected to periodic acid Schiff Staining. Goblet cell density was quantified by J Image software. Quantitative real-time PCR for conjunctival muc 5AC messenger RNA expression was also performed. Results: Sod1 −/− mice had significantly higher fluorescein Staining scores compared to the WT mice before eye drop instillation. The mean tear film breakup time, Rose Bengal Staining scores, and muc5 messenger RNA expression improved significantly with diquafosol treatment in both the WT and the knockout mice. The mean fluorescein Staining score and aqueous tear quantity significantly improved in the Sod1 −/− mice with treatment. A notable and consistent increase in goblet cells and decrease in inflammatory cell infiltrates could be confirmed in all specimens after 2 weeks of diquafosol eye drop application. Conclusions: Three percent diquafosol ophthalmic solution appears to be effective in the treatment of ocular surface disease in this age-related dry eye disease mouse model.
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Immunohistochemistry and electron microscopy of retrocorneal scrolls in syphilitic interstitial keratitis.
Current eye research, 2007Co-Authors: Murat Dogru, Naoko Kato, Yukihiro Matsumoto, Yoichi Tanaka, Naoko Akabane, Shigeto Shimmura, Kazuo Tsubota, Jun ShimazakiAbstract:Purpose: To describe the immunohistochemical and electron microscopic characteristics of retrocorneal scrolls in syphilitic interstitial keratitis. Methods: Five eyes of five patients with congenital syphilitic interstitial keratitis who underwent keratoplasty for corneal opacities and corneal edema were studied. The corneal buttons were processed for histologic examination with hematoxylin and eosin Staining and underwent immunohistochemistry Stainings for collagen types I, III, IV, V, VI, VIII, fibronectin, laminin, and decorin. The corneal buttons were also processed for transmission electron microscopy and immunoelectron microscopy. Results: Light microscopy revealed that the retrocorneal scrolls had a multilayered, amorphous, acellular matrix. All scrolls were lined with attenuated corneal endothelial cells. The Descemet membranes in all specimens had areas of irregular thickening with attenuated endothelium. Immunohistochemical assessment of the scrolls showed positive Staining for collagens I, III,...
Osama M A Ibrahim - One of the best experts on this subject based on the ideXlab platform.
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oxidative stress induced age dependent meibomian gland dysfunction in cu zn superoxide dismutase 1 sod1 knockout mice
PLOS ONE, 2014Co-Authors: Osama M A Ibrahim, Takahiko Shimizu, Murat Dogru, Jun Shimazaki, Takashi Kojima, Yukihiro Matsumoto, Ayako Igarashi, Tais Hitomi Wakamatsu, Takaaki Inaba, Kazuo TsubotaAbstract:Purpose The purpose of our study was to investigate alterations in the meibomian gland (MG) in Cu, Zn-Superoxide Dismutase-1 knockout (Sod1−/−) mouse. Methods Tear function tests [Break up time (BUT) and cotton thread] and ocular vital Staining test were performed on Sod1−/− male mice (n = 24) aged 10 and 50 weeks, and age and sex matched wild–type (+/+) mice (n = 25). Tear and serum samples were collected at sacrifice for inflammatory cytokine assays. MG specimens underwent Hematoxylin and Eosin Staining, Mallory Staining for fibrosis, Oil Red O lipid Staining, TUNEL Staining, immunohistochemistry Stainings for 4HNE, 8-OHdG and CD45. Transmission electron microscopic examination (TEM) was also performed. Results Corneal vital Staining scores in the Sod1−/− mice were significantly higher compared with the wild type mice throughout the follow-up. Tear and serum IL-6 and TNF-α levels also showed significant elevations in the 10 to 50 week Sod1−/− mice. Oil Red O Staining showed an accumulation of large lipid droplets in the Sod1−/− mice at 50 weeks. Immunohistochemistry revealed both increased TUNEL and oxidative stress marker Stainings of the MG acinar epithelium in the Sod1−/− mice compared to the wild type mice. Immunohistochemistry Staining for CD45 showed increasing inflammatory cell infiltrates from 10 to 50 weeks in the Sod1−/− mice compared to the wild type mice. TEM revealed prominent mitochondrial changes in 50 week Sod1−/− mice. Conclusions Our results suggest that reactive oxygen species might play a vital role in the pathogensis of meibomian gland dysfunction. The Sod1−/− mouse appears to be a promising model for the study of reactive oxygen species associated MG alterations.
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the effects of 3 diquafosol sodium application on the tear functions and ocular surface of the cu zn superoxide dismutase 1 sod1 knockout mice
Molecular Vision, 2014Co-Authors: Takashi Kojima, Taeko Nagata, Seika Shimazaki, Osama M A Ibrahim, Kazunari Higa, Yoshiyuki Satake, Takuji Shirasawa, Takahiko Shimizu, Murat Dogru, Jun ShimazakiAbstract:−/− −/− male mice with C57BL/background and 14 eyes of 7 C57BL6 strain wild-type male mice were examined at 40 weeks in this study. All mice had application of 3% diquafosol ophthalmic solution six times a day for 2 weeks. Tear film stability and corneal epithelial damage was evaluated by fluorescein and Rose Bengal Stainings. Anterior segment photography was performed before and after eye drop instillations. Aqueous tear quantity was measured with phenol red–impregnated cotton threads without anesthesia. Animals were sacrificed at 42 weeks after diquafosol treatment and the whole globe specimens were subjected to periodic acid Schiff Staining. Goblet cell density was quantified by J Image software. Quantitative real-time PCR for conjunctival muc 5AC messenger RNA expression was also performed. Results: Sod1 −/− mice had significantly higher fluorescein Staining scores compared to the WT mice before eye drop instillation. The mean tear film breakup time, Rose Bengal Staining scores, and muc5 messenger RNA expression improved significantly with diquafosol treatment in both the WT and the knockout mice. The mean fluorescein Staining score and aqueous tear quantity significantly improved in the Sod1 −/− mice with treatment. A notable and consistent increase in goblet cells and decrease in inflammatory cell infiltrates could be confirmed in all specimens after 2 weeks of diquafosol eye drop application. Conclusions: Three percent diquafosol ophthalmic solution appears to be effective in the treatment of ocular surface disease in this age-related dry eye disease mouse model.
Takashi Kojima - One of the best experts on this subject based on the ideXlab platform.
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oxidative stress induced age dependent meibomian gland dysfunction in cu zn superoxide dismutase 1 sod1 knockout mice
PLOS ONE, 2014Co-Authors: Osama M A Ibrahim, Takahiko Shimizu, Murat Dogru, Jun Shimazaki, Takashi Kojima, Yukihiro Matsumoto, Ayako Igarashi, Tais Hitomi Wakamatsu, Takaaki Inaba, Kazuo TsubotaAbstract:Purpose The purpose of our study was to investigate alterations in the meibomian gland (MG) in Cu, Zn-Superoxide Dismutase-1 knockout (Sod1−/−) mouse. Methods Tear function tests [Break up time (BUT) and cotton thread] and ocular vital Staining test were performed on Sod1−/− male mice (n = 24) aged 10 and 50 weeks, and age and sex matched wild–type (+/+) mice (n = 25). Tear and serum samples were collected at sacrifice for inflammatory cytokine assays. MG specimens underwent Hematoxylin and Eosin Staining, Mallory Staining for fibrosis, Oil Red O lipid Staining, TUNEL Staining, immunohistochemistry Stainings for 4HNE, 8-OHdG and CD45. Transmission electron microscopic examination (TEM) was also performed. Results Corneal vital Staining scores in the Sod1−/− mice were significantly higher compared with the wild type mice throughout the follow-up. Tear and serum IL-6 and TNF-α levels also showed significant elevations in the 10 to 50 week Sod1−/− mice. Oil Red O Staining showed an accumulation of large lipid droplets in the Sod1−/− mice at 50 weeks. Immunohistochemistry revealed both increased TUNEL and oxidative stress marker Stainings of the MG acinar epithelium in the Sod1−/− mice compared to the wild type mice. Immunohistochemistry Staining for CD45 showed increasing inflammatory cell infiltrates from 10 to 50 weeks in the Sod1−/− mice compared to the wild type mice. TEM revealed prominent mitochondrial changes in 50 week Sod1−/− mice. Conclusions Our results suggest that reactive oxygen species might play a vital role in the pathogensis of meibomian gland dysfunction. The Sod1−/− mouse appears to be a promising model for the study of reactive oxygen species associated MG alterations.
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the effects of 3 diquafosol sodium application on the tear functions and ocular surface of the cu zn superoxide dismutase 1 sod1 knockout mice
Molecular Vision, 2014Co-Authors: Takashi Kojima, Taeko Nagata, Seika Shimazaki, Osama M A Ibrahim, Kazunari Higa, Yoshiyuki Satake, Takuji Shirasawa, Takahiko Shimizu, Murat Dogru, Jun ShimazakiAbstract:−/− −/− male mice with C57BL/background and 14 eyes of 7 C57BL6 strain wild-type male mice were examined at 40 weeks in this study. All mice had application of 3% diquafosol ophthalmic solution six times a day for 2 weeks. Tear film stability and corneal epithelial damage was evaluated by fluorescein and Rose Bengal Stainings. Anterior segment photography was performed before and after eye drop instillations. Aqueous tear quantity was measured with phenol red–impregnated cotton threads without anesthesia. Animals were sacrificed at 42 weeks after diquafosol treatment and the whole globe specimens were subjected to periodic acid Schiff Staining. Goblet cell density was quantified by J Image software. Quantitative real-time PCR for conjunctival muc 5AC messenger RNA expression was also performed. Results: Sod1 −/− mice had significantly higher fluorescein Staining scores compared to the WT mice before eye drop instillation. The mean tear film breakup time, Rose Bengal Staining scores, and muc5 messenger RNA expression improved significantly with diquafosol treatment in both the WT and the knockout mice. The mean fluorescein Staining score and aqueous tear quantity significantly improved in the Sod1 −/− mice with treatment. A notable and consistent increase in goblet cells and decrease in inflammatory cell infiltrates could be confirmed in all specimens after 2 weeks of diquafosol eye drop application. Conclusions: Three percent diquafosol ophthalmic solution appears to be effective in the treatment of ocular surface disease in this age-related dry eye disease mouse model.
Kazuo Tsubota - One of the best experts on this subject based on the ideXlab platform.
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oxidative stress induced age dependent meibomian gland dysfunction in cu zn superoxide dismutase 1 sod1 knockout mice
PLOS ONE, 2014Co-Authors: Osama M A Ibrahim, Takahiko Shimizu, Murat Dogru, Jun Shimazaki, Takashi Kojima, Yukihiro Matsumoto, Ayako Igarashi, Tais Hitomi Wakamatsu, Takaaki Inaba, Kazuo TsubotaAbstract:Purpose The purpose of our study was to investigate alterations in the meibomian gland (MG) in Cu, Zn-Superoxide Dismutase-1 knockout (Sod1−/−) mouse. Methods Tear function tests [Break up time (BUT) and cotton thread] and ocular vital Staining test were performed on Sod1−/− male mice (n = 24) aged 10 and 50 weeks, and age and sex matched wild–type (+/+) mice (n = 25). Tear and serum samples were collected at sacrifice for inflammatory cytokine assays. MG specimens underwent Hematoxylin and Eosin Staining, Mallory Staining for fibrosis, Oil Red O lipid Staining, TUNEL Staining, immunohistochemistry Stainings for 4HNE, 8-OHdG and CD45. Transmission electron microscopic examination (TEM) was also performed. Results Corneal vital Staining scores in the Sod1−/− mice were significantly higher compared with the wild type mice throughout the follow-up. Tear and serum IL-6 and TNF-α levels also showed significant elevations in the 10 to 50 week Sod1−/− mice. Oil Red O Staining showed an accumulation of large lipid droplets in the Sod1−/− mice at 50 weeks. Immunohistochemistry revealed both increased TUNEL and oxidative stress marker Stainings of the MG acinar epithelium in the Sod1−/− mice compared to the wild type mice. Immunohistochemistry Staining for CD45 showed increasing inflammatory cell infiltrates from 10 to 50 weeks in the Sod1−/− mice compared to the wild type mice. TEM revealed prominent mitochondrial changes in 50 week Sod1−/− mice. Conclusions Our results suggest that reactive oxygen species might play a vital role in the pathogensis of meibomian gland dysfunction. The Sod1−/− mouse appears to be a promising model for the study of reactive oxygen species associated MG alterations.
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Immunohistochemistry and electron microscopy of retrocorneal scrolls in syphilitic interstitial keratitis.
Current eye research, 2007Co-Authors: Murat Dogru, Naoko Kato, Yukihiro Matsumoto, Yoichi Tanaka, Naoko Akabane, Shigeto Shimmura, Kazuo Tsubota, Jun ShimazakiAbstract:Purpose: To describe the immunohistochemical and electron microscopic characteristics of retrocorneal scrolls in syphilitic interstitial keratitis. Methods: Five eyes of five patients with congenital syphilitic interstitial keratitis who underwent keratoplasty for corneal opacities and corneal edema were studied. The corneal buttons were processed for histologic examination with hematoxylin and eosin Staining and underwent immunohistochemistry Stainings for collagen types I, III, IV, V, VI, VIII, fibronectin, laminin, and decorin. The corneal buttons were also processed for transmission electron microscopy and immunoelectron microscopy. Results: Light microscopy revealed that the retrocorneal scrolls had a multilayered, amorphous, acellular matrix. All scrolls were lined with attenuated corneal endothelial cells. The Descemet membranes in all specimens had areas of irregular thickening with attenuated endothelium. Immunohistochemical assessment of the scrolls showed positive Staining for collagens I, III,...
Takahiko Shimizu - One of the best experts on this subject based on the ideXlab platform.
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oxidative stress induced age dependent meibomian gland dysfunction in cu zn superoxide dismutase 1 sod1 knockout mice
PLOS ONE, 2014Co-Authors: Osama M A Ibrahim, Takahiko Shimizu, Murat Dogru, Jun Shimazaki, Takashi Kojima, Yukihiro Matsumoto, Ayako Igarashi, Tais Hitomi Wakamatsu, Takaaki Inaba, Kazuo TsubotaAbstract:Purpose The purpose of our study was to investigate alterations in the meibomian gland (MG) in Cu, Zn-Superoxide Dismutase-1 knockout (Sod1−/−) mouse. Methods Tear function tests [Break up time (BUT) and cotton thread] and ocular vital Staining test were performed on Sod1−/− male mice (n = 24) aged 10 and 50 weeks, and age and sex matched wild–type (+/+) mice (n = 25). Tear and serum samples were collected at sacrifice for inflammatory cytokine assays. MG specimens underwent Hematoxylin and Eosin Staining, Mallory Staining for fibrosis, Oil Red O lipid Staining, TUNEL Staining, immunohistochemistry Stainings for 4HNE, 8-OHdG and CD45. Transmission electron microscopic examination (TEM) was also performed. Results Corneal vital Staining scores in the Sod1−/− mice were significantly higher compared with the wild type mice throughout the follow-up. Tear and serum IL-6 and TNF-α levels also showed significant elevations in the 10 to 50 week Sod1−/− mice. Oil Red O Staining showed an accumulation of large lipid droplets in the Sod1−/− mice at 50 weeks. Immunohistochemistry revealed both increased TUNEL and oxidative stress marker Stainings of the MG acinar epithelium in the Sod1−/− mice compared to the wild type mice. Immunohistochemistry Staining for CD45 showed increasing inflammatory cell infiltrates from 10 to 50 weeks in the Sod1−/− mice compared to the wild type mice. TEM revealed prominent mitochondrial changes in 50 week Sod1−/− mice. Conclusions Our results suggest that reactive oxygen species might play a vital role in the pathogensis of meibomian gland dysfunction. The Sod1−/− mouse appears to be a promising model for the study of reactive oxygen species associated MG alterations.
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the effects of 3 diquafosol sodium application on the tear functions and ocular surface of the cu zn superoxide dismutase 1 sod1 knockout mice
Molecular Vision, 2014Co-Authors: Takashi Kojima, Taeko Nagata, Seika Shimazaki, Osama M A Ibrahim, Kazunari Higa, Yoshiyuki Satake, Takuji Shirasawa, Takahiko Shimizu, Murat Dogru, Jun ShimazakiAbstract:−/− −/− male mice with C57BL/background and 14 eyes of 7 C57BL6 strain wild-type male mice were examined at 40 weeks in this study. All mice had application of 3% diquafosol ophthalmic solution six times a day for 2 weeks. Tear film stability and corneal epithelial damage was evaluated by fluorescein and Rose Bengal Stainings. Anterior segment photography was performed before and after eye drop instillations. Aqueous tear quantity was measured with phenol red–impregnated cotton threads without anesthesia. Animals were sacrificed at 42 weeks after diquafosol treatment and the whole globe specimens were subjected to periodic acid Schiff Staining. Goblet cell density was quantified by J Image software. Quantitative real-time PCR for conjunctival muc 5AC messenger RNA expression was also performed. Results: Sod1 −/− mice had significantly higher fluorescein Staining scores compared to the WT mice before eye drop instillation. The mean tear film breakup time, Rose Bengal Staining scores, and muc5 messenger RNA expression improved significantly with diquafosol treatment in both the WT and the knockout mice. The mean fluorescein Staining score and aqueous tear quantity significantly improved in the Sod1 −/− mice with treatment. A notable and consistent increase in goblet cells and decrease in inflammatory cell infiltrates could be confirmed in all specimens after 2 weeks of diquafosol eye drop application. Conclusions: Three percent diquafosol ophthalmic solution appears to be effective in the treatment of ocular surface disease in this age-related dry eye disease mouse model.
