The Experts below are selected from a list of 28668 Experts worldwide ranked by ideXlab platform
Minlin Zheng - One of the best experts on this subject based on the ideXlab platform.
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Standard Operating Procedures for Standardized mass rearing of the dengue and chikungunya vectors aedes aegypti and aedes albopictus diptera culicidae ii egg storage and hatching
Parasites & Vectors, 2015Co-Authors: Rosemary Susan Lees, Minlin Zheng, Dongjing Zhang, David Damiens, Jeremie R. L. GillesAbstract:Background Quantification of eggs prior to rearing the immature stages of mosquitoes is an essential step in establishing a Standardized mass rearing system. To develop a simple and accurate method of egg quantification for Aedes aegypti and Aedes albopictus, the relationship between egg number and weight, as well as egg number and volume, were studied.
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Standard Operating Procedures for Standardized mass rearing of the dengue and chikungunya vectors aedes aegypti and aedes albopictus diptera culicidae ii egg storage and hatching
Parasites & Vectors, 2015Co-Authors: Rosemary Susan Lees, Minlin Zheng, Dongjing Zhang, David Damiens, Jeremie R. L. GillesAbstract:Management of large quantities of eggs will be a crucial aspect of the efficient and sustainable mass production of mosquitoes for programmes with a Sterile Insect Technique component. The efficiency of different hatching media and effectiveness of long term storage methods are presented here. The effect on hatch rate of storage duration and three hatching media was analysed: deionized water, boiled deionized water and a bacterial broth, using Two-way ANOVA and Post hoc Tukey tests, and the Pearson correlation coefficient was used to find the effect on the proportion of collapsed eggs. Two long term storage methods were also tested: conventional storage (egg paper strips stored in zip lock bags within a sealed plastic box), and water storage (egg papers in a covered plastic cup with deionized water). Regression analyses were used to find the effect of water storage and storage duration on hatch rate. Both species hatched most efficiently in bacterial broth. Few eggs hatched in deionized water, and pre-boiling the water increased the hatch rate of Ae. aegypti, but not Ae. albopictus. A hatch rate greater than 80 % was obtained after 10 weeks of conventional storage in Ae. aegypti and 11 weeks in Ae. albopictus. After this period, hatching decreased dramatically; no eggs hatched after 24 weeks. Storing eggs in water produced an 85 % hatch rate after 5 months in both species. A small but significant proportion of eggs hatched in the water, probably due to combined effects of natural deoxygenation of the water over time and the natural instalment hatching typical of the species. The demonstrated efficiency of the bacterial broth hatching medium for both Ae. albopictus and Ae. aegypti facilitates mass production of these two important vector species in the same facility, with use of a common hatching medium reducing cost and operational complexity. Similarly the increased hatch rate of eggs stored in water would allow greater flexibility of egg management in a large programme over the medium term, particularly if oxygenation of the water by bubbling oxygen through the storage tray could be applied to prevent hatching during storage.
Jeremie R. L. Gilles - One of the best experts on this subject based on the ideXlab platform.
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Standard Operating Procedures for Standardized mass rearing of the dengue and chikungunya vectors aedes aegypti and aedes albopictus diptera culicidae ii egg storage and hatching
Parasites & Vectors, 2015Co-Authors: Rosemary Susan Lees, Minlin Zheng, Dongjing Zhang, David Damiens, Jeremie R. L. GillesAbstract:Background Quantification of eggs prior to rearing the immature stages of mosquitoes is an essential step in establishing a Standardized mass rearing system. To develop a simple and accurate method of egg quantification for Aedes aegypti and Aedes albopictus, the relationship between egg number and weight, as well as egg number and volume, were studied.
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Standard Operating Procedures for Standardized mass rearing of the dengue and chikungunya vectors aedes aegypti and aedes albopictus diptera culicidae ii egg storage and hatching
Parasites & Vectors, 2015Co-Authors: Rosemary Susan Lees, Minlin Zheng, Dongjing Zhang, David Damiens, Jeremie R. L. GillesAbstract:Management of large quantities of eggs will be a crucial aspect of the efficient and sustainable mass production of mosquitoes for programmes with a Sterile Insect Technique component. The efficiency of different hatching media and effectiveness of long term storage methods are presented here. The effect on hatch rate of storage duration and three hatching media was analysed: deionized water, boiled deionized water and a bacterial broth, using Two-way ANOVA and Post hoc Tukey tests, and the Pearson correlation coefficient was used to find the effect on the proportion of collapsed eggs. Two long term storage methods were also tested: conventional storage (egg paper strips stored in zip lock bags within a sealed plastic box), and water storage (egg papers in a covered plastic cup with deionized water). Regression analyses were used to find the effect of water storage and storage duration on hatch rate. Both species hatched most efficiently in bacterial broth. Few eggs hatched in deionized water, and pre-boiling the water increased the hatch rate of Ae. aegypti, but not Ae. albopictus. A hatch rate greater than 80 % was obtained after 10 weeks of conventional storage in Ae. aegypti and 11 weeks in Ae. albopictus. After this period, hatching decreased dramatically; no eggs hatched after 24 weeks. Storing eggs in water produced an 85 % hatch rate after 5 months in both species. A small but significant proportion of eggs hatched in the water, probably due to combined effects of natural deoxygenation of the water over time and the natural instalment hatching typical of the species. The demonstrated efficiency of the bacterial broth hatching medium for both Ae. albopictus and Ae. aegypti facilitates mass production of these two important vector species in the same facility, with use of a common hatching medium reducing cost and operational complexity. Similarly the increased hatch rate of eggs stored in water would allow greater flexibility of egg management in a large programme over the medium term, particularly if oxygenation of the water by bubbling oxygen through the storage tray could be applied to prevent hatching during storage.
Charles L. Sprung - One of the best experts on this subject based on the ideXlab platform.
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chapter 5 essential equipment pharmaceuticals and supplies recommendations and Standard Operating Procedures for intensive care unit and hospital preparations for an influenza epidemic or mass disaster
Intensive Care Medicine, 2010Co-Authors: Charles L. Sprung, Jozef KeseciogluAbstract:Purpose To provide recommendations and Standard Operating Procedures for intensive care unit and hospital preparations for an influenza pandemic or mass disaster with a specific focus on essential equipment, pharmaceuticals and supplies. Methods Based on a literature review and expert opinion, a Delphi process was used to define the essential topics including essential equipment, pharmaceuticals and supplies. Results Key recommendations include: (1) ensure that adequate essential medical equipment, pharmaceuticals and important supplies are available during a disaster; (2) develop a communication and coordination system between health care facilities and local/regional/state/country governmental authorities for the provision of additional support; (3) determine the required resources, order and stockpile adequate resources, and judiciously distribute them; (4) acquire additional mechanical ventilators that are portable, provide adequate gas exchange for a range of clinical conditions, function with low-flow oxygen and without high pressure, and are safe for patients and staff; (5) provide advanced ventilatory support and rescue therapies including high levels of inspired oxygen and positive end-expiratory pressure, volume and pressure control ventilation, inhaled nitric oxide, high-frequency ventilation, prone positioning ventilation and extracorporeal membrane oxygenation; (6) triage scarce resources including equipment, pharmaceuticals and supplies based on those who are likely to benefit most or on a 'first come, first served' basis. Conclusions Judicious planning and adoption of protocols for providing adequate equipment, pharmaceuticals and supplies are necessary to optimize outcomes during a pandemic.
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chapter 1 introduction recommendations and Standard Operating Procedures for intensive care unit and hospital preparations for an influenza epidemic or mass disaster
Intensive Care Medicine, 2010Co-Authors: Janice L Zimmerman, Charles L. SprungAbstract:Purpose To provide recommendations and Standard Operating Procedures (SOPs) for intensive care unit (ICU) and hospital preparations for an influenza pandemic or mass disaster with focus on education of all stakeholders, specifically the emergency executive control groups, ICU staff and staff co-opted to assist with patient management. Methods Based on a literature review and expert opinion, a Delphi process was used to define the essential topics, including staff education. Results Key recommendations include: (1) define functional roles and responsibilities of the internal personnel and interface agencies or sectors; (2) determine logistic support and requirements necessary for the effective implementation of the SOPs; (3) determine what is required to maintain the SOPs; (4) recommended training and activities include: (a) personal protection techniques; (b) environmental contamination; (c) medical management; (d) laboratory specimens; (e) alert lists; (f) training of recruited staff; (g) ethical issues; (h) psychosocial issues; (i) dealing with the deceased; (j) policies for restricting visitors; (k) mechanisms for enforcing policies; (5) Training should begin as soon as possible with daily demonstrations followed by supervised practice; (6) identify the staff to participate in training programs, verify that they have participated and evaluate their knowledge subsequently. Conclusions Judicious planning and adoption of protocols for staff education are necessary to optimize outcomes during a pandemic.
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chapter 6 protection of patients and staff during a pandemic recommendations and Standard Operating Procedures for intensive care unit and hospital preparations for an influenza epidemic or mass disaster
Intensive Care Medicine, 2010Co-Authors: Bruce Taylor, Hugh Montgomery, Andrew Rhodes, Charles L. SprungAbstract:Purpose To provide recommendations and Standard Operating Procedures (SOPs) for intensive care unit (ICU) and hospital preparations for an influenza pandemic or mass disaster with a specific focus on protection of patients and staff.
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Chapter 2. Surge capacity and infrastructure considerations for mass critical care. Recommendations and Standard Operating Procedures for intensive care unit and hospital preparations for an influenza epidemic or mass disaster.
Intensive care medicine, 2010Co-Authors: John L. Hick, Michael D. Christian, Charles L. SprungAbstract:To provide recommendations and Standard Operating Procedures for intensive care unit (ICU) and hospital preparations for a mass disaster or influenza epidemic with a specific focus on surge capacity and infrastructure considerations. Based on a literature review and expert opinion, a Delphi process was used to define the essential topics including surge capacity and infrastructure considerations. Key recommendations include: (1) hospitals should increase their ICU beds to the maximal extent by expanding ICU capacity and expanding ICUs into other areas; (2) hospitals should have appropriate beds and monitors for these expansion areas; hospitals should develop contingency plans at the facility and government (local, state, provincial, national) levels to provide additional ventilators; (3) hospitals should develop a phased staffing plan (nursing and physician) for ICUs that provides sufficient patient care supervision during contingency and crisis situations; (4) hospitals should provide expert input to the emergency management personnel at the hospital both during planning for surge capacity as well as during response; (5) hospitals should assure that adequate infrastructure support is present to support critical care activities; (6) hospitals should prioritize locations for expansion by expanding existing ICUs, using postanesthesia care units and emergency departments to capacity, then step-down units, large procedure suites, telemetry units and finally hospital wards. Judicious planning and adoption of protocols for surge capacity and infrastructure considerations are necessary to optimize outcomes during a pandemic.
Dongjing Zhang - One of the best experts on this subject based on the ideXlab platform.
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Standard Operating Procedures for Standardized mass rearing of the dengue and chikungunya vectors aedes aegypti and aedes albopictus diptera culicidae ii egg storage and hatching
Parasites & Vectors, 2015Co-Authors: Rosemary Susan Lees, Minlin Zheng, Dongjing Zhang, David Damiens, Jeremie R. L. GillesAbstract:Background Quantification of eggs prior to rearing the immature stages of mosquitoes is an essential step in establishing a Standardized mass rearing system. To develop a simple and accurate method of egg quantification for Aedes aegypti and Aedes albopictus, the relationship between egg number and weight, as well as egg number and volume, were studied.
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Standard Operating Procedures for Standardized mass rearing of the dengue and chikungunya vectors aedes aegypti and aedes albopictus diptera culicidae ii egg storage and hatching
Parasites & Vectors, 2015Co-Authors: Rosemary Susan Lees, Minlin Zheng, Dongjing Zhang, David Damiens, Jeremie R. L. GillesAbstract:Management of large quantities of eggs will be a crucial aspect of the efficient and sustainable mass production of mosquitoes for programmes with a Sterile Insect Technique component. The efficiency of different hatching media and effectiveness of long term storage methods are presented here. The effect on hatch rate of storage duration and three hatching media was analysed: deionized water, boiled deionized water and a bacterial broth, using Two-way ANOVA and Post hoc Tukey tests, and the Pearson correlation coefficient was used to find the effect on the proportion of collapsed eggs. Two long term storage methods were also tested: conventional storage (egg paper strips stored in zip lock bags within a sealed plastic box), and water storage (egg papers in a covered plastic cup with deionized water). Regression analyses were used to find the effect of water storage and storage duration on hatch rate. Both species hatched most efficiently in bacterial broth. Few eggs hatched in deionized water, and pre-boiling the water increased the hatch rate of Ae. aegypti, but not Ae. albopictus. A hatch rate greater than 80 % was obtained after 10 weeks of conventional storage in Ae. aegypti and 11 weeks in Ae. albopictus. After this period, hatching decreased dramatically; no eggs hatched after 24 weeks. Storing eggs in water produced an 85 % hatch rate after 5 months in both species. A small but significant proportion of eggs hatched in the water, probably due to combined effects of natural deoxygenation of the water over time and the natural instalment hatching typical of the species. The demonstrated efficiency of the bacterial broth hatching medium for both Ae. albopictus and Ae. aegypti facilitates mass production of these two important vector species in the same facility, with use of a common hatching medium reducing cost and operational complexity. Similarly the increased hatch rate of eggs stored in water would allow greater flexibility of egg management in a large programme over the medium term, particularly if oxygenation of the water by bubbling oxygen through the storage tray could be applied to prevent hatching during storage.
Rosemary Susan Lees - One of the best experts on this subject based on the ideXlab platform.
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Standard Operating Procedures for Standardized mass rearing of the dengue and chikungunya vectors aedes aegypti and aedes albopictus diptera culicidae ii egg storage and hatching
Parasites & Vectors, 2015Co-Authors: Rosemary Susan Lees, Minlin Zheng, Dongjing Zhang, David Damiens, Jeremie R. L. GillesAbstract:Background Quantification of eggs prior to rearing the immature stages of mosquitoes is an essential step in establishing a Standardized mass rearing system. To develop a simple and accurate method of egg quantification for Aedes aegypti and Aedes albopictus, the relationship between egg number and weight, as well as egg number and volume, were studied.
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Standard Operating Procedures for Standardized mass rearing of the dengue and chikungunya vectors aedes aegypti and aedes albopictus diptera culicidae ii egg storage and hatching
Parasites & Vectors, 2015Co-Authors: Rosemary Susan Lees, Minlin Zheng, Dongjing Zhang, David Damiens, Jeremie R. L. GillesAbstract:Management of large quantities of eggs will be a crucial aspect of the efficient and sustainable mass production of mosquitoes for programmes with a Sterile Insect Technique component. The efficiency of different hatching media and effectiveness of long term storage methods are presented here. The effect on hatch rate of storage duration and three hatching media was analysed: deionized water, boiled deionized water and a bacterial broth, using Two-way ANOVA and Post hoc Tukey tests, and the Pearson correlation coefficient was used to find the effect on the proportion of collapsed eggs. Two long term storage methods were also tested: conventional storage (egg paper strips stored in zip lock bags within a sealed plastic box), and water storage (egg papers in a covered plastic cup with deionized water). Regression analyses were used to find the effect of water storage and storage duration on hatch rate. Both species hatched most efficiently in bacterial broth. Few eggs hatched in deionized water, and pre-boiling the water increased the hatch rate of Ae. aegypti, but not Ae. albopictus. A hatch rate greater than 80 % was obtained after 10 weeks of conventional storage in Ae. aegypti and 11 weeks in Ae. albopictus. After this period, hatching decreased dramatically; no eggs hatched after 24 weeks. Storing eggs in water produced an 85 % hatch rate after 5 months in both species. A small but significant proportion of eggs hatched in the water, probably due to combined effects of natural deoxygenation of the water over time and the natural instalment hatching typical of the species. The demonstrated efficiency of the bacterial broth hatching medium for both Ae. albopictus and Ae. aegypti facilitates mass production of these two important vector species in the same facility, with use of a common hatching medium reducing cost and operational complexity. Similarly the increased hatch rate of eggs stored in water would allow greater flexibility of egg management in a large programme over the medium term, particularly if oxygenation of the water by bubbling oxygen through the storage tray could be applied to prevent hatching during storage.
