The Experts below are selected from a list of 1416 Experts worldwide ranked by ideXlab platform
Mohamed Belmekki - One of the best experts on this subject based on the ideXlab platform.
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value of xpert mrsa sa blood culture assay on the gene xpert dx system for rapid detection of staphylococcus aureus and coagulase negative staphylococci in patients with Staphylococcal Bacteremia
Diagnostic Microbiology and Infectious Disease, 2013Co-Authors: Maurice Biendo, Hedi Mammeri, Emilie Pluquet, Helene Guillon, F Rousseau, Brigitte Canarelli, Mohamed BelmekkiAbstract:The Xpert MRSA/SA BC assay was examined prospectively in patients with Staphylococcal Bacteremia including 6 patients with blood culture bottles inoculated with biological fluid (synovial fluid in 4 cases and peritoneal fluid in 2 cases). Among the 56 Staphylococci species isolated, 80.3% were coagulase-negative staphylococci (CoNS) and 19.7% were S. aureus. Methicillin susceptibility test results showed that 77.8% of isolates were methicillin-resistant CoNS (MRCoNS) and 22.2% of isolates were methicillin-susceptible CoNS (MSCoNS). Of 11 S. aureus isolates, 63.7% were methicillin-susceptible S. aureus (MSSA) and 36.3% were methicillin-resistant S. aureus (MRSA). Xpert MRSA/SA BC results showed that genotypic results were in concordance with phenotypic results in 94.6% of cases versus 5.4% of discordant cases. Of these 3 discordant cases, 1 S. aureus isolate had an MRSA phenotype and an SPA(+)mec(+)SCCmec(-) genotype and another S. aureus isolate was phenotypically MSSA and genotypically SPA(+)mec(+)SCCmec(-), and 1 S. epidermidis isolate was phenotypically MSCoNS and genotypically SPA(-)mec(+)SCCmec(-).
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Value of Xpert MRSA/SA blood culture assay on the Gene Xpert® Dx System for rapid detection of Staphylococcus aureus and coagulase-negative staphylococci in patients with Staphylococcal Bacteremia
Diagnostic microbiology and infectious disease, 2012Co-Authors: Maurice Biendo, Hedi Mammeri, Emilie Pluquet, Helene Guillon, F Rousseau, Brigitte Canarelli, Mohamed BelmekkiAbstract:The Xpert MRSA/SA BC assay was examined prospectively in patients with Staphylococcal Bacteremia including 6 patients with blood culture bottles inoculated with biological fluid (synovial fluid in 4 cases and peritoneal fluid in 2 cases). Among the 56 Staphylococci species isolated, 80.3% were coagulase-negative staphylococci (CoNS) and 19.7% were S. aureus. Methicillin susceptibility test results showed that 77.8% of isolates were methicillin-resistant CoNS (MRCoNS) and 22.2% of isolates were methicillin-susceptible CoNS (MSCoNS). Of 11 S. aureus isolates, 63.7% were methicillin-susceptible S. aureus (MSSA) and 36.3% were methicillin-resistant S. aureus (MRSA). Xpert MRSA/SA BC results showed that genotypic results were in concordance with phenotypic results in 94.6% of cases versus 5.4% of discordant cases. Of these 3 discordant cases, 1 S. aureus isolate had an MRSA phenotype and an SPA(+)mec(+)SCCmec(-) genotype and another S. aureus isolate was phenotypically MSSA and genotypically SPA(+)mec(+)SCCmec(-), and 1 S. epidermidis isolate was phenotypically MSCoNS and genotypically SPA(-)mec(+)SCCmec(-).
Maurice Biendo - One of the best experts on this subject based on the ideXlab platform.
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Value of molecular typing in the assessment of bacterial translocation during coagulase-negative Staphylococcal Bacteremia in preterm infants
2019Co-Authors: Andre Leke, Géraldine Amar, Bertin Elion Dzon, Gilles Morin, Sabrina Goudjil, Guy Kongolo, Maurice BiendoAbstract:Abstract Background One hundred fifty-seven preterm infants enrolled in the study were hospitalized between 2012-2014 at Amiens-Picardie University Hospital.Only 28 (17.8%) of these children who had experienced at least one episode of secondary Coagulase-negative Staphylococcal Bacteremia with concomitant positive stool cultures were included in this study.The purpose of this study was to assess the rate of intestinal bacterial translocation associated with these infections. Methods Blood cultures and stool cultures were performed in the context of this study. All isolates of Staphylococcus spp were examined by MALDI-TOF MS. Antibiotic susceptibility and genotyping were also performed. Results Sixteen resistance patterns were identified from blood and stool based on antibiotic susceptibility testing. Ten of the Coagulase-negative Staphylococcus strains isolated from blood samples exhibited R pattern e (35.7%) and eleven of the Coagulase-negative Staphylococcus strains isolated from stool samples exhibited R pattern e (39.2%). Blood culture results were concordant with stool culture results in 53.5% of cases and discordant in 46.5% of cases.Fifteen isolates exhibited three ERIC-2 (A, B, C) and three RAPD-PCR (D, E, F) patterns. ERIC-2 patterns comprised A ( S. epidermidis isolates); B ( S. haemolyticus isolates) and C ( unidentified Coagulase-negative Staphylococcus isolates). RAPD patterns consisted of D ( unidentified Coagulase-negative Staphylococcus isolates), E ( S. haemolyticus isolates), and F ( S. epidermidis isolates). Conclusion Bacterial translocation from the intestinal tract was likely source of Coagulase-negative Staphylococcal Bacteremia in hospitalized preterm infants.
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value of xpert mrsa sa blood culture assay on the gene xpert dx system for rapid detection of staphylococcus aureus and coagulase negative staphylococci in patients with Staphylococcal Bacteremia
Diagnostic Microbiology and Infectious Disease, 2013Co-Authors: Maurice Biendo, Hedi Mammeri, Emilie Pluquet, Helene Guillon, F Rousseau, Brigitte Canarelli, Mohamed BelmekkiAbstract:The Xpert MRSA/SA BC assay was examined prospectively in patients with Staphylococcal Bacteremia including 6 patients with blood culture bottles inoculated with biological fluid (synovial fluid in 4 cases and peritoneal fluid in 2 cases). Among the 56 Staphylococci species isolated, 80.3% were coagulase-negative staphylococci (CoNS) and 19.7% were S. aureus. Methicillin susceptibility test results showed that 77.8% of isolates were methicillin-resistant CoNS (MRCoNS) and 22.2% of isolates were methicillin-susceptible CoNS (MSCoNS). Of 11 S. aureus isolates, 63.7% were methicillin-susceptible S. aureus (MSSA) and 36.3% were methicillin-resistant S. aureus (MRSA). Xpert MRSA/SA BC results showed that genotypic results were in concordance with phenotypic results in 94.6% of cases versus 5.4% of discordant cases. Of these 3 discordant cases, 1 S. aureus isolate had an MRSA phenotype and an SPA(+)mec(+)SCCmec(-) genotype and another S. aureus isolate was phenotypically MSSA and genotypically SPA(+)mec(+)SCCmec(-), and 1 S. epidermidis isolate was phenotypically MSCoNS and genotypically SPA(-)mec(+)SCCmec(-).
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Value of Xpert MRSA/SA blood culture assay on the Gene Xpert® Dx System for rapid detection of Staphylococcus aureus and coagulase-negative staphylococci in patients with Staphylococcal Bacteremia
Diagnostic microbiology and infectious disease, 2012Co-Authors: Maurice Biendo, Hedi Mammeri, Emilie Pluquet, Helene Guillon, F Rousseau, Brigitte Canarelli, Mohamed BelmekkiAbstract:The Xpert MRSA/SA BC assay was examined prospectively in patients with Staphylococcal Bacteremia including 6 patients with blood culture bottles inoculated with biological fluid (synovial fluid in 4 cases and peritoneal fluid in 2 cases). Among the 56 Staphylococci species isolated, 80.3% were coagulase-negative staphylococci (CoNS) and 19.7% were S. aureus. Methicillin susceptibility test results showed that 77.8% of isolates were methicillin-resistant CoNS (MRCoNS) and 22.2% of isolates were methicillin-susceptible CoNS (MSCoNS). Of 11 S. aureus isolates, 63.7% were methicillin-susceptible S. aureus (MSSA) and 36.3% were methicillin-resistant S. aureus (MRSA). Xpert MRSA/SA BC results showed that genotypic results were in concordance with phenotypic results in 94.6% of cases versus 5.4% of discordant cases. Of these 3 discordant cases, 1 S. aureus isolate had an MRSA phenotype and an SPA(+)mec(+)SCCmec(-) genotype and another S. aureus isolate was phenotypically MSSA and genotypically SPA(+)mec(+)SCCmec(-), and 1 S. epidermidis isolate was phenotypically MSCoNS and genotypically SPA(-)mec(+)SCCmec(-).
Neil M. Ampel - One of the best experts on this subject based on the ideXlab platform.
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Scoring the Risk for Endocarditis During Staphylococcal Bacteremia
NEJM Journal Watch, 2020Co-Authors: Neil M. AmpelAbstract:Predicting which patients with Staphylococcus aureus Bacteremia (SAB) will develop infective endocarditis (IE) can be difficult. Transesophageal
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A Scoring System for Staphylococcal Bacteremia
NEJM Journal Watch, 2015Co-Authors: Neil M. AmpelAbstract:Staphylococcus aureus Bacteremia (SAB) may result in infective endocarditis (IE). Although transesophageal echocardiography (TEE) is considered the
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Occult Staphylococcal Bacteremia in the Emergency Department
NEJM Journal Watch, 2012Co-Authors: Neil M. AmpelAbstract:Because current blood-culture technology does not allow for immediate results, patients may be discharged from the emergency department (ED) and
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Infectious Diseases Consultation for Staphylococcal Bacteremia
NEJM Journal Watch, 2010Co-Authors: Neil M. AmpelAbstract:Mortality rates for patients with Staphylococcus aureus Bacteremia range from 20% to 50%. Appropriate choice and duration of antimicrobial therapy,
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Staphylococcal Bacteremia After Removal of Colonized CVCs
NEJM Journal Watch, 2007Co-Authors: Neil M. AmpelAbstract:No treatment protocol exists to guide the management of patients whose central venous catheters (CVCs), following removal, are found to be colonized
Helene Guillon - One of the best experts on this subject based on the ideXlab platform.
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value of xpert mrsa sa blood culture assay on the gene xpert dx system for rapid detection of staphylococcus aureus and coagulase negative staphylococci in patients with Staphylococcal Bacteremia
Diagnostic Microbiology and Infectious Disease, 2013Co-Authors: Maurice Biendo, Hedi Mammeri, Emilie Pluquet, Helene Guillon, F Rousseau, Brigitte Canarelli, Mohamed BelmekkiAbstract:The Xpert MRSA/SA BC assay was examined prospectively in patients with Staphylococcal Bacteremia including 6 patients with blood culture bottles inoculated with biological fluid (synovial fluid in 4 cases and peritoneal fluid in 2 cases). Among the 56 Staphylococci species isolated, 80.3% were coagulase-negative staphylococci (CoNS) and 19.7% were S. aureus. Methicillin susceptibility test results showed that 77.8% of isolates were methicillin-resistant CoNS (MRCoNS) and 22.2% of isolates were methicillin-susceptible CoNS (MSCoNS). Of 11 S. aureus isolates, 63.7% were methicillin-susceptible S. aureus (MSSA) and 36.3% were methicillin-resistant S. aureus (MRSA). Xpert MRSA/SA BC results showed that genotypic results were in concordance with phenotypic results in 94.6% of cases versus 5.4% of discordant cases. Of these 3 discordant cases, 1 S. aureus isolate had an MRSA phenotype and an SPA(+)mec(+)SCCmec(-) genotype and another S. aureus isolate was phenotypically MSSA and genotypically SPA(+)mec(+)SCCmec(-), and 1 S. epidermidis isolate was phenotypically MSCoNS and genotypically SPA(-)mec(+)SCCmec(-).
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Value of Xpert MRSA/SA blood culture assay on the Gene Xpert® Dx System for rapid detection of Staphylococcus aureus and coagulase-negative staphylococci in patients with Staphylococcal Bacteremia
Diagnostic microbiology and infectious disease, 2012Co-Authors: Maurice Biendo, Hedi Mammeri, Emilie Pluquet, Helene Guillon, F Rousseau, Brigitte Canarelli, Mohamed BelmekkiAbstract:The Xpert MRSA/SA BC assay was examined prospectively in patients with Staphylococcal Bacteremia including 6 patients with blood culture bottles inoculated with biological fluid (synovial fluid in 4 cases and peritoneal fluid in 2 cases). Among the 56 Staphylococci species isolated, 80.3% were coagulase-negative staphylococci (CoNS) and 19.7% were S. aureus. Methicillin susceptibility test results showed that 77.8% of isolates were methicillin-resistant CoNS (MRCoNS) and 22.2% of isolates were methicillin-susceptible CoNS (MSCoNS). Of 11 S. aureus isolates, 63.7% were methicillin-susceptible S. aureus (MSSA) and 36.3% were methicillin-resistant S. aureus (MRSA). Xpert MRSA/SA BC results showed that genotypic results were in concordance with phenotypic results in 94.6% of cases versus 5.4% of discordant cases. Of these 3 discordant cases, 1 S. aureus isolate had an MRSA phenotype and an SPA(+)mec(+)SCCmec(-) genotype and another S. aureus isolate was phenotypically MSSA and genotypically SPA(+)mec(+)SCCmec(-), and 1 S. epidermidis isolate was phenotypically MSCoNS and genotypically SPA(-)mec(+)SCCmec(-).
Brigitte Canarelli - One of the best experts on this subject based on the ideXlab platform.
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value of xpert mrsa sa blood culture assay on the gene xpert dx system for rapid detection of staphylococcus aureus and coagulase negative staphylococci in patients with Staphylococcal Bacteremia
Diagnostic Microbiology and Infectious Disease, 2013Co-Authors: Maurice Biendo, Hedi Mammeri, Emilie Pluquet, Helene Guillon, F Rousseau, Brigitte Canarelli, Mohamed BelmekkiAbstract:The Xpert MRSA/SA BC assay was examined prospectively in patients with Staphylococcal Bacteremia including 6 patients with blood culture bottles inoculated with biological fluid (synovial fluid in 4 cases and peritoneal fluid in 2 cases). Among the 56 Staphylococci species isolated, 80.3% were coagulase-negative staphylococci (CoNS) and 19.7% were S. aureus. Methicillin susceptibility test results showed that 77.8% of isolates were methicillin-resistant CoNS (MRCoNS) and 22.2% of isolates were methicillin-susceptible CoNS (MSCoNS). Of 11 S. aureus isolates, 63.7% were methicillin-susceptible S. aureus (MSSA) and 36.3% were methicillin-resistant S. aureus (MRSA). Xpert MRSA/SA BC results showed that genotypic results were in concordance with phenotypic results in 94.6% of cases versus 5.4% of discordant cases. Of these 3 discordant cases, 1 S. aureus isolate had an MRSA phenotype and an SPA(+)mec(+)SCCmec(-) genotype and another S. aureus isolate was phenotypically MSSA and genotypically SPA(+)mec(+)SCCmec(-), and 1 S. epidermidis isolate was phenotypically MSCoNS and genotypically SPA(-)mec(+)SCCmec(-).
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Value of Xpert MRSA/SA blood culture assay on the Gene Xpert® Dx System for rapid detection of Staphylococcus aureus and coagulase-negative staphylococci in patients with Staphylococcal Bacteremia
Diagnostic microbiology and infectious disease, 2012Co-Authors: Maurice Biendo, Hedi Mammeri, Emilie Pluquet, Helene Guillon, F Rousseau, Brigitte Canarelli, Mohamed BelmekkiAbstract:The Xpert MRSA/SA BC assay was examined prospectively in patients with Staphylococcal Bacteremia including 6 patients with blood culture bottles inoculated with biological fluid (synovial fluid in 4 cases and peritoneal fluid in 2 cases). Among the 56 Staphylococci species isolated, 80.3% were coagulase-negative staphylococci (CoNS) and 19.7% were S. aureus. Methicillin susceptibility test results showed that 77.8% of isolates were methicillin-resistant CoNS (MRCoNS) and 22.2% of isolates were methicillin-susceptible CoNS (MSCoNS). Of 11 S. aureus isolates, 63.7% were methicillin-susceptible S. aureus (MSSA) and 36.3% were methicillin-resistant S. aureus (MRSA). Xpert MRSA/SA BC results showed that genotypic results were in concordance with phenotypic results in 94.6% of cases versus 5.4% of discordant cases. Of these 3 discordant cases, 1 S. aureus isolate had an MRSA phenotype and an SPA(+)mec(+)SCCmec(-) genotype and another S. aureus isolate was phenotypically MSSA and genotypically SPA(+)mec(+)SCCmec(-), and 1 S. epidermidis isolate was phenotypically MSCoNS and genotypically SPA(-)mec(+)SCCmec(-).