The Experts below are selected from a list of 96 Experts worldwide ranked by ideXlab platform

Mohamed N. Seleem - One of the best experts on this subject based on the ideXlab platform.

  • Antibacterial activity and therapeutic efficacy of Fl-P(R)P(R)P(L)-5, a cationic amphiphilic polyproline helix, in a mouse model of Staphylococcal Skin Infection.
    Drug design development and therapy, 2015
    Co-Authors: Shankar Thangamani, Manish Nepal, Jean Chmielewski, Mohamed N. Seleem
    Abstract:

    The antibacterial activities and therapeutic efficacy of the cationic, unnatural proline-rich peptide Fl-P(R)P(R)P(L)-5 were evaluated against multidrug-resistant Staphylococcus aureus in a mouse model of Skin Infection. Fl-P(R)P(R)P(L)-5 showed potent activity against all clinical isolates of S. aureus tested, including methicillin- and vancomycin-resistant S. aureus (MRSA and VRSA, respectively). Fl-P(R)P(R)P(L)-5 was also superior in clearing established in vitro biofilms of S. aureus and Staphylococcus epidermidis, compared with the established antimicrobials mupirocin and vancomycin. Additionally, topical treatment of an MRSA-infected wound with Fl-P(R)P(R)P(L)-5 enhanced wound closure and significantly reduced bacterial load. Finally, 0.5% Fl-P(R)P(R)P(L)-5 significantly reduced the levels of the inflammatory cytokines tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and interleukin-1 beta (IL-1β) in wounds induced by MRSA Skin Infection. In conclusion, the results of this study suggest the potential application of Fl-P(R)P(R)P(L)-5 in the treatment of Staphylococcal Skin Infections.

  • Efficacy of short novel antimicrobial and anti-inflammatory peptides in a mouse model of methicillin-resistant Staphylococcus aureus (MRSA) Skin Infection
    Drug design development and therapy, 2014
    Co-Authors: Mohamed F. Mohamed, Mohamed N. Seleem
    Abstract:

    The therapeutic efficacy of two novel short antimicrobial and anti-inflammatory peptides (RR and RRIKA) was evaluated in a mouse model of Staphylococcal Skin Infection. RR (2%) and RRIKA (2%) significantly reduced the bacterial counts and the levels of proinflammatory cytokines, tumor necrosis factor (TNF)-α, and interleukin (IL)-6, in methicillin-resistant Staphylococcus aureus USA 300-0114 Skin lesions. Furthermore, the combined therapy of RRIKA (1%) and lysostaphin (0.5%) had significantly higher antiStaphylococcal and anti-inflammatory activity compared to monotherapy. This study supports the potential use of these peptides for topical treatment of methicillin-resistant Staphylococcus aureus Skin Infections.

Jiang Xia - One of the best experts on this subject based on the ideXlab platform.

  • antibacterial photodynamic peptides for Staphylococcal Skin Infection
    Biomaterials Science, 2020
    Co-Authors: Xiaoling Lei, Lin Qiu, Min Lan, Shuwen Zhou, Pengfei Cui, Ronghui Zheng, Pengju Jiang, Jianhao Wang, Jiang Xia
    Abstract:

    As the barrier between the human body and the outside world, the Skin is vulnerable to pathogenic microorganisms, especially when suffering from Skin injuries such as burns. Staphylococcus aureus remains the most common type of bacteria that infects humans, and the surging drug resistance poses a major threat to the treatment of these Infections. Here we report the development of antibacterial photodynamic peptides (APPs) that are constructed based on the covalent conjugation of an antibacterial peptide and the photosensitizer chlorin e6 (Ce6). Peptide conjugation significantly increases the photo-stability of Ce6, while retaining its ROS generation capability under photo-irradiation. The APPs combine the antibacterial activity of the peptide and the photodynamic therapy of Ce6, and under the assistance of mild laser irradiation, can eradicate bacterial Infection and inhibit the formation of bacterial biofilms ex vivo. One of the APPs, (GKRWWKWWRR)2KGGK(Ce6)G, AMP2-Ce6, with Ce6 conjugated with the dimeric peptide, showed exceptional antibacterial activity with an MIC90 value around 3.2 μM without photo-irradiation and <0.1 μM with short light treatment. Supported by a hydrogel matrix composed of gelatin and recombinant human collagen III protein (rhCol III) mimicking the extracellular matrix of Skin cells, AMP2-Ce6 efficiently accelerated the healing rate of wounds and improved the quality of wound healing in mice infected with Staphylococcus aureus. Altogether, here we report the development of antibacterial photodynamic peptides, which together with a regenerative matrix material exhibit an added effect against Staphylococcal Skin Infection. This composite material holds promise as a new type of wound dressing material for Skin Infection and wound healing.

  • Antibacterial photodynamic peptides for Staphylococcal Skin Infection.
    Biomaterials science, 2020
    Co-Authors: Lei Xiaoling, Lin Qiu, Ronghui Zheng, Jianhao Wang, Lan Min, Zhou Shuwen, Cui Pengfei, Jiang Pengju, Jiang Xia
    Abstract:

    As the barrier between the human body and the outside world, the Skin is vulnerable to pathogenic microorganisms, especially when suffering from Skin injuries such as burns. Staphylococcus aureus remains the most common type of bacteria that infects humans, and the surging drug resistance poses a major threat to the treatment of these Infections. Here we report the development of antibacterial photodynamic peptides (APPs) that are constructed based on the covalent conjugation of an antibacterial peptide and the photosensitizer chlorin e6 (Ce6). Peptide conjugation significantly increases the photo-stability of Ce6, while retaining its ROS generation capability under photo-irradiation. The APPs combine the antibacterial activity of the peptide and the photodynamic therapy of Ce6, and under the assistance of mild laser irradiation, can eradicate bacterial Infection and inhibit the formation of bacterial biofilms ex vivo. One of the APPs, (GKRWWKWWRR)2KGGK(Ce6)G, AMP2-Ce6, with Ce6 conjugated with the dimeric peptide, showed exceptional antibacterial activity with an MIC90 value around 3.2 μM without photo-irradiation and

Frank R. Deleo - One of the best experts on this subject based on the ideXlab platform.

  • Panton-Valentine Leukocidin Is Not a Virulence Determinant in Murine Models of Community-Associated Methicillin-Resistant Staphylococcus aureus Disease
    The Journal of infectious diseases, 2008
    Co-Authors: Juliane Bubeck Wardenburg, Amy M. Palazzolo-ballance, Michael Otto, Olaf Schneewind, Frank R. Deleo
    Abstract:

    Increases in the incidence and severity of community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) Infections have spawned efforts to define unique virulence properties among prevalent strains. Panton-Valentine leukocidin (PVL), a pore-forming cytotoxin, has garnered attention because of its epidemiologic association with CA-MRSA. Using both the clinical isolate LAC, which is representative of the epidemic USA300 strain, and its isogenic PVL-negative strain in murine models of Staphylococcal Skin Infection and pneumonia, we expanded upon recent studies by assessing the contribution of PVL in the genetic background of BALB/c mice. The data presented in this report support the observation that PVL does not contribute to the pathogenesis of Staphylococcal Infection of mice.

Mohamed F. Mohamed - One of the best experts on this subject based on the ideXlab platform.

Juliane Bubeck Wardenburg - One of the best experts on this subject based on the ideXlab platform.

  • Panton-Valentine Leukocidin Is Not a Virulence Determinant in Murine Models of Community-Associated Methicillin-Resistant Staphylococcus aureus Disease
    The Journal of infectious diseases, 2008
    Co-Authors: Juliane Bubeck Wardenburg, Amy M. Palazzolo-ballance, Michael Otto, Olaf Schneewind, Frank R. Deleo
    Abstract:

    Increases in the incidence and severity of community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) Infections have spawned efforts to define unique virulence properties among prevalent strains. Panton-Valentine leukocidin (PVL), a pore-forming cytotoxin, has garnered attention because of its epidemiologic association with CA-MRSA. Using both the clinical isolate LAC, which is representative of the epidemic USA300 strain, and its isogenic PVL-negative strain in murine models of Staphylococcal Skin Infection and pneumonia, we expanded upon recent studies by assessing the contribution of PVL in the genetic background of BALB/c mice. The data presented in this report support the observation that PVL does not contribute to the pathogenesis of Staphylococcal Infection of mice.