Starch Production

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Jiangning Ai - One of the best experts on this subject based on the ideXlab platform.

  • salinity manipulation as an effective method for enhanced Starch Production in the marine microalga tetraselmis subcordiformis
    Bioresource Technology, 2013
    Co-Authors: Jiangning Ai
    Abstract:

    Microalgal Starch is considered a promising feedstock for bioethanol Production. The biomass and Starch accumulation in the marine microalga Tetraselmis subcordiformis were characterized under different salinities in response to nitrogen repletion (+N) or depletion (-N) at high irradiance (HI) or low irradiance (LI). Under favorable nutritional conditions (HI+N), biomass accumulation was seldom affected under 20% normal salinity, though Starch accumulation were somewhat reduced. Increased salinity impaired overall biomass and Starch accumulation, though it led to a temporary Starch accumulation at initial cultivation phase. Under nitrogen deprivation, decreased salinity strengthened biomass and Starch accumulation regardless of irradiance. The highest Starch content of 58.2% dry weight and Starch productivity of 0.62 g L-1 d(-1) were obtained under HI-N with 20% normal salinity. Decreased salinity combined with -N generated moderate stress to facilitate Starch accumulation. Salinity manipulation can be effectively applied for enhanced Starch Production in marine microalgae. (c) 2013 Elsevier Ltd. All rights reserved.

  • Characterization of cell growth and Starch Production in the marine green microalga Tetraselmis subcordiformis under extracellular phosphorus-deprived and sequentially phosphorus-replete conditions
    Applied Microbiology and Biotechnology, 2013
    Co-Authors: Jiangning Ai
    Abstract:

    Microalgal Starch is a potential feedstock for biofuel Production. Nutrient stress is widely used to stimulate Starch accumulation in microalgae. Cell growth and Starch accumulation in the marine green microalga Tetraselmis subcordiformis were evaluated under extracellular phosphorus deprivation with initial cell densities (ICD) of 1.5, 3.0, 6.0, and 9.0 × 106 cells mL−1. The intracellular stored phosphorus supported cell growth when extracellular phosphorus was absent. The maximum Starch content of 44.1 % was achieved in the lowest ICD culture, while the maximum biomass productivity of 0.71 g L−1 day−1, Starch concentration of 1.6 g L−1, and Starch productivity of 0.30 g L−1 day−1 were all obtained in the culture with the ICD of 3.0 × 106 cells mL−1. Appropriate ICD could be used to regulate the intracellular phosphorus concentration and maintain adequate photosynthetic activity to achieve the highest Starch productivity, along with biomass and Starch concentration. The recovery of phosphorus-deprived T. subcordiformis in medium containing 0.5, 1.0, or 6.0 mM KH2PO4 was also tested. Cell growth and Starch accumulation ability could be recovered completely. A phosphorus pool in T. subcordiformis was shown to manipulate its metabolic activity under different environmental phosphorus availability. Though lower Starch productivity and Starch content were achieved under phosphorus deprivation compared with nitrogen- or sulfur-deprived conditions, the higher biomass and Starch concentration make T. subcordiformis a good candidate for biomass and Starch Production under extracellular phosphorus deprivation.

  • enhancing Starch Production of a marine green microalga tetraselmis subcordiformis through nutrient limitation
    Bioresource Technology, 2012
    Co-Authors: Jiangning Ai, Wei Zhang
    Abstract:

    Abstract Microalgal Starch is a potential feedstock for biofuel Production. The effects of KNO 3 and MgSO 4 concentrations and light intensity on biomass and Starch Production by the marine microalga, Tetraselmis subcordiformis , were investigated. Under 200 μmol m −2  s −1 irradiance and sulfur-deprived conditions, a Starch productivity of 0.62 g L −1  d −1 and a Starch content of 62.1% based on dry weight (DW) was achieved. A Starch content of 54.3% was achieved under low irradiance and nitrogen starvation, which was 6.5% higher than that under nutrient- and light-sufficient conditions. Photosynthetic activity was indispensable for Starch accumulation. It is difficult to reach high Starch productivity and Starch concentration simultaneously. Proper nutrient concentrations are necessary to achieve high Starch productivity or Starch concentration based on the target. The high Starch productivity and Starch content suggest that T. subcordiformis is a promising microalgal Starch producer.

Wei Zhang - One of the best experts on this subject based on the ideXlab platform.

  • enhancing Starch Production of a marine green microalga tetraselmis subcordiformis through nutrient limitation
    Bioresource Technology, 2012
    Co-Authors: Jiangning Ai, Wei Zhang
    Abstract:

    Abstract Microalgal Starch is a potential feedstock for biofuel Production. The effects of KNO 3 and MgSO 4 concentrations and light intensity on biomass and Starch Production by the marine microalga, Tetraselmis subcordiformis , were investigated. Under 200 μmol m −2  s −1 irradiance and sulfur-deprived conditions, a Starch productivity of 0.62 g L −1  d −1 and a Starch content of 62.1% based on dry weight (DW) was achieved. A Starch content of 54.3% was achieved under low irradiance and nitrogen starvation, which was 6.5% higher than that under nutrient- and light-sufficient conditions. Photosynthetic activity was indispensable for Starch accumulation. It is difficult to reach high Starch productivity and Starch concentration simultaneously. Proper nutrient concentrations are necessary to achieve high Starch productivity or Starch concentration based on the target. The high Starch productivity and Starch content suggest that T. subcordiformis is a promising microalgal Starch producer.

Barahima Abbas - One of the best experts on this subject based on the ideXlab platform.

  • short communication a comparative study of phenotypes and Starch Production in sago palm metroxylon sagu growing naturally in temporarily inundated and non inundated areas of south sorong indonesia
    Biodiversitas, 2019
    Co-Authors: Tomas Yater, Herman Wafom Tubur, Cipta Meliala, Barahima Abbas
    Abstract:

    Abstract. Yater T, Tubur HW, Meliala C, Abbas B. 2019. Short Communication:  A comparative study of phenotypes and Starch Production in sago palm (Metroxylon sagu) growing naturally in temporarily inundated and non-inundated areas of South Sorong, Indonesia. Biodiversitas 20:  1121-1126. Sago palm forests and sago palm semi cultivation are generally spread in swampy areas, seasonally inundated areas and non-inundated areas. The objectives of this study are to determine and compare the phenotypes of and Starch Production by sago palms growing naturally in the temporarily inundated areas (TIA type) and non-inundated areas (WIA type) in South Sorong District, West Papua Province, Indonesia. Sago palms of both habitats were found to be the same variety based on analysis of vernacular names and general characteristics. Comparison of morphological characters related to Starch Production of TIA and WIA types showed that there were no significant differences between the two studied types. The distribution of Starch along the sago trunk was observed to be uneven, higher Starch accumulation was found in the middle part of the trunk which was significantly different from the lower and upper parts of the trunk.

  • assessment genetic variation and relationship of sago palm metroxylon sagu rottb in indonesia based on specific expression gene wx genes markers
    African Journal of Plant Science, 2012
    Co-Authors: Barahima Abbas, H Ehara
    Abstract:

    Starch Production of sago palm based on physical and chemical properties were observed in a large variation in the previous study. The important gene markers used forthe assessment of the genetic variation and relationships which is related to the Starch Production is wx gene markers. The generating data based on the Wx gene markers were described in 8 alleles and 14 genotypes on sago palm in Indonesia. The sizes of alleles range from 100 to 700 bp. Many vernacular names were given by local people where the population is located. However it has different names, but it is genetically the same. The vernacular names do not accurately describe the types of the sago palm. The genetic relationships of sago palm were observed in a higher variation in the level of individual sampling stages, then followed by the populations sampling and the islands sampling stages. Sago palm samples were clustered into four groups in the level of individuals, three groups in the level of populations, and two groups in the level of islands. The existence of the specific genotypes in both population, Serui in the Papua islands and Palopo in the Sulawesi islands are considered to be germplasm resources. The Papua islands have the largest genotype; therefore, it is proposed to be the center of genetic diversity of sago palm in Indonesia.   Key words: Sago palm, wx gene, population, genetic variation, genetic relationship.

Gongke Zhou - One of the best experts on this subject based on the ideXlab platform.

  • the influence of light intensity and photoperiod on duckweed biomass and Starch accumulation for bioethanol Production
    Bioresource Technology, 2015
    Co-Authors: Changjiang Yu, Jinshan Zhao, Li Yu, Gongke Zhou
    Abstract:

    Duckweed has been considered as a valuable feedstock for bioethanol Production due to its high biomass and Starch Production. To investigate the effects of light conditions on duckweed biomass and Starch Production, Lemna aequinoctialis 6000 was cultivated at different photoperiods (12:12, 16:8 and 24:0 h) and light intensities (20, 50, 80, 110, 200 and 400 mu mol m(-2) s(-1)). The results showed that the duckweed biomass and Starch Production was increased with increasing light intensity and photoperiod except at 200 and 400 mu mol m(-2) s(-1). Considering the light cost, 110 mu mol m(-2) s(-1) was optimum light condition for Starch accumulation with the highest maximum growth rate, biomass and Starch Production of 8.90 g m(-2) day(-1), 233.25 g m(-2) and 98.70 g m(-2), respectively. Moreover, the results suggested that high light induction was a promising method for duckweed Starch accumulation. This study provides optimized light conditions for future industrial large-scale duckweed cultivation. (C) 2015 Elsevier Ltd. All rights reserved.

  • The influence of light intensity and photoperiod on duckweed biomass and Starch accumulation for bioethanol Production
    Bioresource Technology, 2015
    Co-Authors: Yehu Yin, Jinshan Zhao, Yubin Ma, Changjiang Sun, Changjiang Yu, Li Yu, Gongke Zhou
    Abstract:

    Duckweed has been considered as a valuable feedstock for bioethanol Production due to its high biomass and Starch Production. To investigate the effects of light conditions on duckweed biomass and Starch Production, Lemna aequinoctialis 6000 was cultivated at different photoperiods (12:12, 16:8 and 24:0h) and light intensities (20, 50, 80, 110, 200 and 400μmolm-2s-1). The results showed that the duckweed biomass and Starch Production was increased with increasing light intensity and photoperiod except at 200 and 400μmolm-2s-1. Considering the light cost, 110μmolm-2s-1was optimum light condition for Starch accumulation with the highest maximum growth rate, biomass and Starch Production of 8.90gm-2day-1, 233.25gm-2and 98.70gm-2, respectively. Moreover, the results suggested that high light induction was a promising method for duckweed Starch accumulation. This study provides optimized light conditions for future industrial large-scale duckweed cultivation.

Yongkui Zhang - One of the best experts on this subject based on the ideXlab platform.

  • Application of an in situ CO 2 –bicarbonate system under nitrogen depletion to improve photosynthetic biomass and Starch Production and regulate amylose accumulation in a marine green microalga Tetraselmis subcordiformis
    Biotechnology for Biofuels, 2019
    Co-Authors: Man Qi, Bobo Liang, Qi Xiang, Yongkui Zhang
    Abstract:

    Microalgal Starch is regarded as a promising alternative to crop-based Starch for biorefinery such as the Production of biofuels and bio-based chemicals. The single or separate use of inorganic carbon source, e.g., CO2 and NaHCO3, caused aberrant pH, which restricts the biomass and Starch Production. The present study applied an in situ CO2–NaHCO3 system to regulate photosynthetic biomass and Starch Production along with Starch quality in a marine green microalga Tetraselmis subcordiformis under nitrogen-depletion (−N) and nitrogen-limitation (±N) conditions. The CO2 (2%)–NaHCO3 (1 g L−1) system stabilized the pH at 7.7 in the −N cultivation, under which the optimal biomass and Starch accumulation were achieved. The biomass and Starch productivity under −N were improved by 2.1-fold and 1.7-fold, respectively, with 1 g L−1 NaHCO3 addition compared with the one without NaHCO3 addition. NaHCO3 addition alleviated the high-dCO2 inhibition caused by the single CO2 aeration, and provided sufficient effective carbon source HCO3− for the maintenance of adequate photosynthetic efficiency and increase in photoprotection to facilitate the biomass and Starch Production. The amylose content was also increased by 44% under this CO2–bicarbonate system compared to the single use of CO2. The highest Starch productivity of 0.73 g L−1 day−1 under −N cultivation and highest Starch concentration of 4.14 g L−1 under ±N cultivation were both achieved with the addition of 1 g L−1 NaHCO3. These levels were comparable to or exceeded the current achievements reported in studies. The addition of 5 g L−1 NaHCO3 under ±N cultivation led to a Production of high-amylose Starch (59.3% of total Starch), which could be used as a source of functional food. The in situ CO2–NaHCO3 system significantly improved the biomass and Starch Production in T. subcordiformis. It could also regulate the Starch quality with varied relative amylose content under different cultivation modes for diverse downstream applications that could promote the economic feasibility of microalgal Starch-based biofuel Production. Adoption of this system in T. subcordiformis would facilitate the CO2 mitigation couple with its Starch-based biorefinery.

  • Phosphorus Enhances Photosynthetic Storage Starch Production in a Green Microalga (Chlorophyta) Tetraselmis subcordiformis in Nitrogen Starvation Conditions.
    Journal of Agricultural and Food Chemistry, 2018
    Co-Authors: Junpeng Jiang, Yongkui Zhang
    Abstract:

    Microalgae are potential Starch producers as alternatives to agricultural crops. This study disclosed the effects and mechanism of phosphorus availability exerted on storage Starch Production in a ...