Stem Borer

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Yinchang Wang - One of the best experts on this subject based on the ideXlab platform.

V Girijashankar - One of the best experts on this subject based on the ideXlab platform.

  • development of transgenic sorghum for insect resistance against the spotted Stem Borer chilo partellus
    Plant Cell Reports, 2005
    Co-Authors: H. C. Sharma, V Girijashankar, Kiran K Sharma, V Swathisree, Sivarama L Prasad, B V Bhat, Monique Royer, Blanca San Secundo, Lakshmi M Narasu
    Abstract:

    Transgenic sorghum plants expressing a synthetic cry1Ac gene from Bacillus thuringiensis (Bt) under the control of a wound-inducible promoter from the maize protease inhibitor gene (mpiC1) were produced via particle bombardment of shoot apices. Plants were regenerated from the transformed shoot apices via direct somatic embryogenesis with an intermittent three-step selection strategy using the herbicide Basta. Molecular characterisation based on polymerase chain reaction and Southern blot analysis revealed multiple insertions of the cry1Ac gene in five plants from three independent transformation events. Inheritance and expression of the Bt gene was confirmed in T1 plants. Enzyme-linked immunosorbant assay indicated that Cry1Ac protein accumulated at levels of 1–8 ng per gram of fresh tissue in leaves that were mechanically wounded. Transgenic sorghum plants were evaluated for resistance against the spotted Stem Borer (Chilo partellus Swinhoe) in insect bioassays, which indicated partial resistance to damage by the neonate larvae of the spotted Stem Borer. Reduction in leaf damage 5 days after infestation was up to 60%; larval mortality was 40%, with the surviving larvae showing a 36% reduction in weight over those fed on control plants. Despite the low levels of expression of Bt δ-endotoxin under the control of the wound-inducible promoter, the transgenic plants showed partial tolerance against first instar larvae of the spotted Stem Borer.

Sharmistha Barthakur - One of the best experts on this subject based on the ideXlab platform.

  • overview on current status of biotechnological interventions on yellow Stem Borer scirpophaga incertulas lepidoptera crambidae resistance in rice
    Biotechnology Advances, 2010
    Co-Authors: Sikha Deka, Sharmistha Barthakur
    Abstract:

    Yellow Stem Borer (YSB), Scirpophaga incertulas (Lepidoptera: Crambidae), a monophagous pest of paddy is considered as most important pest of rain fed low land and flood prone rice eco-syStems. Breeding of yellow Stem Borer resistance in rice is difficult owing to the complex genetics of the trait, inherent difficulties in screening and poor understanding of the genetics of resistance. On the other hand, a good level of resistance against the widespread yellow Stem Borer has been rare in the rice germplasm. Resistance to insects has been demonstrated in transgenic plants expressing genes for δ-endotoxins from Bacillus thuringiensis (Bt), protease inhibitors, enzymes and plant lectins. The performance of insect resistant GM rice in trials in China has been quite impressive. The present review is an attempt to assess the current state of development in biotechnological intervention for yellow Stem Borer resistance in rice.

Shunfan Wu - One of the best experts on this subject based on the ideXlab platform.

Zhaoguo Han - One of the best experts on this subject based on the ideXlab platform.