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Elaine Soares Coimbra - One of the best experts on this subject based on the ideXlab platform.

  • erratum to vosalophen a vanadium complex with a Stilbene Derivative induction of apoptosis autophagy and efficiency in experimental cutaneous leishmaniasis
    Journal of Biological Inorganic Chemistry, 2017
    Co-Authors: Patricia A Machado, Gustavo S G De Carvalho, Adilson David Da Silva, Alexandre Cuin, Wallace P. Lima, Gilson C. Macedo, Elizandra A. Britta, Celso V. Nakamura, Jessica O F Moraes, Elaine Soares Coimbra
    Abstract:

    In our previous work, we demonstrated the promising in vitro effect of VOSalophen, a vanadium complex with a Stilbene Derivative, against Leishmania amazonensis. Its antileishmanial activity has been associated with oxidative stress in L. amazonensis promastigotes and L. amazonensis-infected macrophages. In the present study, the mechanism involved in the death of parasites after treatment with VOSalophen, as well as in vivo effect in the murine model cutaneous leishmaniasis, has been investigated. Promastigotes of L. amazonensis treated with VOSalophen presented apoptotic cells features, such as cell volume decrease, phosphatidylserine externalization, and DNA fragmentation. An increase in autophagic vacuoles formation in treated promastigotes was also observed, showing that autophagy also may be involved in the death of these parasites. In intracellular amastigotes, DNA fragmentation was observed after treatment with VOSalophen, but this effect was not observed in host cells, highlighting the selective effect of this vanadium complex. In addition, VOSalophen showed activity in the murine model of cutaneous leishmaniasis, without hepatic and renal damages. The outcome described here points out that VOSalophen had promising antileishmanial properties and these data also contribute to the understanding of the mechanisms involved in the death of protozoa induced by metal complexes.

  • high selective antileishmanial activity of vanadium complex with Stilbene Derivative
    Acta Tropica, 2015
    Co-Authors: Patricia A Machado, Vinicius Zamprogno Mota, Ana Clara De Lima Cavalli, Gustavo S G De Carvalho, Adilson David Da Silva, Jacy Gameiro, Alexandre Cuin, Elaine Soares Coimbra
    Abstract:

    Leishmaniasis is a group of disease caused by different species of the parasite Leishmania affecting millions of people worldwide. Conventional therapy relies on multiple parenteral injections with pentavalent antimonials which exhibit high toxicity and various side effects have been reported. Hence, the research for an effective and low toxic effect drug is necessary. In the present work, the synthesis, spectroscopic and analytical characterizations of Stilbene Derivative (H2Salophen) and its vanadium complex (VOSalophen) are reported. Besides the chemical ancillary information, investigation of the leishmanicidal effects of these compounds were provided. The biological assays against promastigote and amastigote forms of L. amazonensis have been shown that VOSalophen exhibited a strong antiparasitic activity (IC50 of 6.65 and 3.51 μM, respectively). Furthermore, the leishmanicidal activity was concentration and time-dependent. Regarding toxicity and selectivity on mammalian cells, VOSalophen have not caused significant damage to human erythrocytes in all concentrations tested and VOSalophen was almost seven times more destructive for the intracellular parasite than for macrophages. Furthermore, the leishmanicidal activity of VOSalophen in promastigote forms of L. amazonensis could be associated to mitochondrial dysfunction and increase of the reactive oxygen species (ROS) production. In L. amazonensis-infected macrophages, VOSalophen induces ROS production and a microbicidal action NO-dependent. Our biological results indicate the effective and selective action of VOSalophen against L. amazonensis and the leishmanicidal effect can be associated to parasite disorders and immumodulatory effects.

Gustavo S G De Carvalho - One of the best experts on this subject based on the ideXlab platform.

  • VOSalophen: a vanadium complex with a Stilbene Derivative—induction of apoptosis, autophagy, and efficiency in experimental cutaneous leishmaniasis
    JBIC Journal of Biological Inorganic Chemistry, 2017
    Co-Authors: Patrícia De A. Machado, Gustavo S G De Carvalho, Alexandre Cuin, Jessica O. F. Morais, Wallace P. Lima, Gilson C. Macedo, Elizandra A. Britta, Celso V. Nakamura, Adilson D. Silva, Elaine S. Coimbra
    Abstract:

    In our previous work, we demonstrated the promising in vitro effect of VOSalophen, a vanadium complex with a Stilbene Derivative, against Leishmania amazonensis . Its antileishmanial activity has been associated with oxidative stress in L. amazonensis promastigotes and L. amazonensis -infected macrophages. In the present study, the mechanism involved in the death of parasites after treatment with VOSalophen, as well as in vivo effect in the murine model cutaneous leishmaniasis, has been investigated. Promastigotes of L. amazonensis treated with VOSalophen presented apoptotic cells features, such as cell volume decrease, phosphatidylserine externalization, and DNA fragmentation. An increase in autophagic vacuoles formation in treated promastigotes was also observed, showing that autophagy also may be involved in the death of these parasites. In intracellular amastigotes, DNA fragmentation was observed after treatment with VOSalophen, but this effect was not observed in host cells, highlighting the selective effect of this vanadium complex. In addition, VOSalophen showed activity in the murine model of cutaneous leishmaniasis, without hepatic and renal damages. The outcome described here points out that VOSalophen had promising antileishmanial properties and these data also contribute to the understanding of the mechanisms involved in the death of protozoa induced by metal complexes.

  • erratum to vosalophen a vanadium complex with a Stilbene Derivative induction of apoptosis autophagy and efficiency in experimental cutaneous leishmaniasis
    Journal of Biological Inorganic Chemistry, 2017
    Co-Authors: Patricia A Machado, Gustavo S G De Carvalho, Adilson David Da Silva, Alexandre Cuin, Wallace P. Lima, Gilson C. Macedo, Elizandra A. Britta, Celso V. Nakamura, Jessica O F Moraes, Elaine Soares Coimbra
    Abstract:

    In our previous work, we demonstrated the promising in vitro effect of VOSalophen, a vanadium complex with a Stilbene Derivative, against Leishmania amazonensis. Its antileishmanial activity has been associated with oxidative stress in L. amazonensis promastigotes and L. amazonensis-infected macrophages. In the present study, the mechanism involved in the death of parasites after treatment with VOSalophen, as well as in vivo effect in the murine model cutaneous leishmaniasis, has been investigated. Promastigotes of L. amazonensis treated with VOSalophen presented apoptotic cells features, such as cell volume decrease, phosphatidylserine externalization, and DNA fragmentation. An increase in autophagic vacuoles formation in treated promastigotes was also observed, showing that autophagy also may be involved in the death of these parasites. In intracellular amastigotes, DNA fragmentation was observed after treatment with VOSalophen, but this effect was not observed in host cells, highlighting the selective effect of this vanadium complex. In addition, VOSalophen showed activity in the murine model of cutaneous leishmaniasis, without hepatic and renal damages. The outcome described here points out that VOSalophen had promising antileishmanial properties and these data also contribute to the understanding of the mechanisms involved in the death of protozoa induced by metal complexes.

  • high selective antileishmanial activity of vanadium complex with Stilbene Derivative
    Acta Tropica, 2015
    Co-Authors: Patricia A Machado, Vinicius Zamprogno Mota, Ana Clara De Lima Cavalli, Gustavo S G De Carvalho, Adilson David Da Silva, Jacy Gameiro, Alexandre Cuin, Elaine Soares Coimbra
    Abstract:

    Leishmaniasis is a group of disease caused by different species of the parasite Leishmania affecting millions of people worldwide. Conventional therapy relies on multiple parenteral injections with pentavalent antimonials which exhibit high toxicity and various side effects have been reported. Hence, the research for an effective and low toxic effect drug is necessary. In the present work, the synthesis, spectroscopic and analytical characterizations of Stilbene Derivative (H2Salophen) and its vanadium complex (VOSalophen) are reported. Besides the chemical ancillary information, investigation of the leishmanicidal effects of these compounds were provided. The biological assays against promastigote and amastigote forms of L. amazonensis have been shown that VOSalophen exhibited a strong antiparasitic activity (IC50 of 6.65 and 3.51 μM, respectively). Furthermore, the leishmanicidal activity was concentration and time-dependent. Regarding toxicity and selectivity on mammalian cells, VOSalophen have not caused significant damage to human erythrocytes in all concentrations tested and VOSalophen was almost seven times more destructive for the intracellular parasite than for macrophages. Furthermore, the leishmanicidal activity of VOSalophen in promastigote forms of L. amazonensis could be associated to mitochondrial dysfunction and increase of the reactive oxygen species (ROS) production. In L. amazonensis-infected macrophages, VOSalophen induces ROS production and a microbicidal action NO-dependent. Our biological results indicate the effective and selective action of VOSalophen against L. amazonensis and the leishmanicidal effect can be associated to parasite disorders and immumodulatory effects.

Alexandre Cuin - One of the best experts on this subject based on the ideXlab platform.

  • VOSalophen: a vanadium complex with a Stilbene Derivative—induction of apoptosis, autophagy, and efficiency in experimental cutaneous leishmaniasis
    JBIC Journal of Biological Inorganic Chemistry, 2017
    Co-Authors: Patrícia De A. Machado, Gustavo S G De Carvalho, Alexandre Cuin, Jessica O. F. Morais, Wallace P. Lima, Gilson C. Macedo, Elizandra A. Britta, Celso V. Nakamura, Adilson D. Silva, Elaine S. Coimbra
    Abstract:

    In our previous work, we demonstrated the promising in vitro effect of VOSalophen, a vanadium complex with a Stilbene Derivative, against Leishmania amazonensis . Its antileishmanial activity has been associated with oxidative stress in L. amazonensis promastigotes and L. amazonensis -infected macrophages. In the present study, the mechanism involved in the death of parasites after treatment with VOSalophen, as well as in vivo effect in the murine model cutaneous leishmaniasis, has been investigated. Promastigotes of L. amazonensis treated with VOSalophen presented apoptotic cells features, such as cell volume decrease, phosphatidylserine externalization, and DNA fragmentation. An increase in autophagic vacuoles formation in treated promastigotes was also observed, showing that autophagy also may be involved in the death of these parasites. In intracellular amastigotes, DNA fragmentation was observed after treatment with VOSalophen, but this effect was not observed in host cells, highlighting the selective effect of this vanadium complex. In addition, VOSalophen showed activity in the murine model of cutaneous leishmaniasis, without hepatic and renal damages. The outcome described here points out that VOSalophen had promising antileishmanial properties and these data also contribute to the understanding of the mechanisms involved in the death of protozoa induced by metal complexes.

  • erratum to vosalophen a vanadium complex with a Stilbene Derivative induction of apoptosis autophagy and efficiency in experimental cutaneous leishmaniasis
    Journal of Biological Inorganic Chemistry, 2017
    Co-Authors: Patricia A Machado, Gustavo S G De Carvalho, Adilson David Da Silva, Alexandre Cuin, Wallace P. Lima, Gilson C. Macedo, Elizandra A. Britta, Celso V. Nakamura, Jessica O F Moraes, Elaine Soares Coimbra
    Abstract:

    In our previous work, we demonstrated the promising in vitro effect of VOSalophen, a vanadium complex with a Stilbene Derivative, against Leishmania amazonensis. Its antileishmanial activity has been associated with oxidative stress in L. amazonensis promastigotes and L. amazonensis-infected macrophages. In the present study, the mechanism involved in the death of parasites after treatment with VOSalophen, as well as in vivo effect in the murine model cutaneous leishmaniasis, has been investigated. Promastigotes of L. amazonensis treated with VOSalophen presented apoptotic cells features, such as cell volume decrease, phosphatidylserine externalization, and DNA fragmentation. An increase in autophagic vacuoles formation in treated promastigotes was also observed, showing that autophagy also may be involved in the death of these parasites. In intracellular amastigotes, DNA fragmentation was observed after treatment with VOSalophen, but this effect was not observed in host cells, highlighting the selective effect of this vanadium complex. In addition, VOSalophen showed activity in the murine model of cutaneous leishmaniasis, without hepatic and renal damages. The outcome described here points out that VOSalophen had promising antileishmanial properties and these data also contribute to the understanding of the mechanisms involved in the death of protozoa induced by metal complexes.

  • high selective antileishmanial activity of vanadium complex with Stilbene Derivative
    Acta Tropica, 2015
    Co-Authors: Patricia A Machado, Vinicius Zamprogno Mota, Ana Clara De Lima Cavalli, Gustavo S G De Carvalho, Adilson David Da Silva, Jacy Gameiro, Alexandre Cuin, Elaine Soares Coimbra
    Abstract:

    Leishmaniasis is a group of disease caused by different species of the parasite Leishmania affecting millions of people worldwide. Conventional therapy relies on multiple parenteral injections with pentavalent antimonials which exhibit high toxicity and various side effects have been reported. Hence, the research for an effective and low toxic effect drug is necessary. In the present work, the synthesis, spectroscopic and analytical characterizations of Stilbene Derivative (H2Salophen) and its vanadium complex (VOSalophen) are reported. Besides the chemical ancillary information, investigation of the leishmanicidal effects of these compounds were provided. The biological assays against promastigote and amastigote forms of L. amazonensis have been shown that VOSalophen exhibited a strong antiparasitic activity (IC50 of 6.65 and 3.51 μM, respectively). Furthermore, the leishmanicidal activity was concentration and time-dependent. Regarding toxicity and selectivity on mammalian cells, VOSalophen have not caused significant damage to human erythrocytes in all concentrations tested and VOSalophen was almost seven times more destructive for the intracellular parasite than for macrophages. Furthermore, the leishmanicidal activity of VOSalophen in promastigote forms of L. amazonensis could be associated to mitochondrial dysfunction and increase of the reactive oxygen species (ROS) production. In L. amazonensis-infected macrophages, VOSalophen induces ROS production and a microbicidal action NO-dependent. Our biological results indicate the effective and selective action of VOSalophen against L. amazonensis and the leishmanicidal effect can be associated to parasite disorders and immumodulatory effects.

Patricia A Machado - One of the best experts on this subject based on the ideXlab platform.

  • erratum to vosalophen a vanadium complex with a Stilbene Derivative induction of apoptosis autophagy and efficiency in experimental cutaneous leishmaniasis
    Journal of Biological Inorganic Chemistry, 2017
    Co-Authors: Patricia A Machado, Gustavo S G De Carvalho, Adilson David Da Silva, Alexandre Cuin, Wallace P. Lima, Gilson C. Macedo, Elizandra A. Britta, Celso V. Nakamura, Jessica O F Moraes, Elaine Soares Coimbra
    Abstract:

    In our previous work, we demonstrated the promising in vitro effect of VOSalophen, a vanadium complex with a Stilbene Derivative, against Leishmania amazonensis. Its antileishmanial activity has been associated with oxidative stress in L. amazonensis promastigotes and L. amazonensis-infected macrophages. In the present study, the mechanism involved in the death of parasites after treatment with VOSalophen, as well as in vivo effect in the murine model cutaneous leishmaniasis, has been investigated. Promastigotes of L. amazonensis treated with VOSalophen presented apoptotic cells features, such as cell volume decrease, phosphatidylserine externalization, and DNA fragmentation. An increase in autophagic vacuoles formation in treated promastigotes was also observed, showing that autophagy also may be involved in the death of these parasites. In intracellular amastigotes, DNA fragmentation was observed after treatment with VOSalophen, but this effect was not observed in host cells, highlighting the selective effect of this vanadium complex. In addition, VOSalophen showed activity in the murine model of cutaneous leishmaniasis, without hepatic and renal damages. The outcome described here points out that VOSalophen had promising antileishmanial properties and these data also contribute to the understanding of the mechanisms involved in the death of protozoa induced by metal complexes.

  • high selective antileishmanial activity of vanadium complex with Stilbene Derivative
    Acta Tropica, 2015
    Co-Authors: Patricia A Machado, Vinicius Zamprogno Mota, Ana Clara De Lima Cavalli, Gustavo S G De Carvalho, Adilson David Da Silva, Jacy Gameiro, Alexandre Cuin, Elaine Soares Coimbra
    Abstract:

    Leishmaniasis is a group of disease caused by different species of the parasite Leishmania affecting millions of people worldwide. Conventional therapy relies on multiple parenteral injections with pentavalent antimonials which exhibit high toxicity and various side effects have been reported. Hence, the research for an effective and low toxic effect drug is necessary. In the present work, the synthesis, spectroscopic and analytical characterizations of Stilbene Derivative (H2Salophen) and its vanadium complex (VOSalophen) are reported. Besides the chemical ancillary information, investigation of the leishmanicidal effects of these compounds were provided. The biological assays against promastigote and amastigote forms of L. amazonensis have been shown that VOSalophen exhibited a strong antiparasitic activity (IC50 of 6.65 and 3.51 μM, respectively). Furthermore, the leishmanicidal activity was concentration and time-dependent. Regarding toxicity and selectivity on mammalian cells, VOSalophen have not caused significant damage to human erythrocytes in all concentrations tested and VOSalophen was almost seven times more destructive for the intracellular parasite than for macrophages. Furthermore, the leishmanicidal activity of VOSalophen in promastigote forms of L. amazonensis could be associated to mitochondrial dysfunction and increase of the reactive oxygen species (ROS) production. In L. amazonensis-infected macrophages, VOSalophen induces ROS production and a microbicidal action NO-dependent. Our biological results indicate the effective and selective action of VOSalophen against L. amazonensis and the leishmanicidal effect can be associated to parasite disorders and immumodulatory effects.

Mang Wang - One of the best experts on this subject based on the ideXlab platform.

  • synthesis and hole transport property of a novel triphenylamine substituted Stilbene Derivative
    Materials Chemistry and Physics, 2004
    Co-Authors: Hongzheng Chen, Mang Wang
    Abstract:

    Abstract A novel hole transport material (HTM) of triphenylamine substituted Stilbene Derivative of 4,4′-bis-[2-[4-[ N -(phenyl-4-methoxyphenyl)-amino]-phenyl-1-yl]-vinyl-1-yl]-1,1′-biphenyl (DSPAVBi) was synthesized. The chemical structure was characterized by IR spectrum, 1 H NMR spectroscopy and elemental analysis. The electronic structure was studied by UV–vis absorption and cyclic voltammetry methods. The photosensitivity of the dual-layer photoreceptor containing oxotitanium phthalocyanine (TiOPC) as the charge generation material (CGM) and DSPAVBi as the charge transportation material (CTM) was higher than that containing its parent compound 4,4′-bis-[2-[4-( N , N -diphenylamino)-phenyl-1-yl]-vinyl-1-yl]-1,1′-biphenyl (DPAVBi) as CTM, in agreement with the results calculated with semi-empirical PM3 method.