Stirred Tank

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Yajie Tang - One of the best experts on this subject based on the ideXlab platform.

  • lycopene production from synthetic medium by blakeslea trispora nrrl 2895 and 2896 in a Stirred Tank fermenter
    Bioprocess and Biosystems Engineering, 2012
    Co-Authors: Hongmei Li, Yajie Tang
    Abstract:

    The dissolved oxygen tension of 20% of air saturation, pH-shift from 4.0 to 5.5 on day 3, and a moderate shear stress (calculated as an impeller tip speed, \( V_{\text{tip}} = 0. 9 2 6- 2. 1 6 1 \, {\text{m}}/{\text{s}} \)) were identified to be the key factors in scaling-up the mated fermentation of Blakeslea trispora NRRL 2895 (+) and 2896 (−) for lycopene production from a shake flask to a Stirred-Tank fermenter. The maximal lycopene production of 183.3 mg/L was obtained in 7.5-L Stirred-Tank fermenter, and then the mated fermentation process was successfully step-wise scaled-up from 7.5- to 200-L Stirred-Tank fermenter. The comparability of the fermentation process was well controlled and the lycopene production was maintained during the process scale-up. Furthermore, with the integrated addition of 150 μmol/L abscisic acid on day 3, 0.5 g/L leucine and 0.1 g/L penicillin on day 4, the highest lycopene production of 270.3 mg/L was achieved in the mated fermentation of B. trispora in Stirred-Tank fermenter.

  • Lycopene production from synthetic medium by Blakeslea trispora NRRL 2895 (+) and 2896 (-) in a Stirred-Tank fermenter.
    Bioprocess and Biosystems Engineering, 2011
    Co-Authors: Xiu-ji Liu, Rui-sang Liu, Yajie Tang
    Abstract:

    The dissolved oxygen tension of 20% of air saturation, pH-shift from 4.0 to 5.5 on day 3, and a moderate shear stress (calculated as an impeller tip speed, \( V_{\text{tip}} = 0. 9 2 6- 2. 1 6 1 \, {\text{m}}/{\text{s}} \)) were identified to be the key factors in scaling-up the mated fermentation of Blakeslea trispora NRRL 2895 (+) and 2896 (−) for lycopene production from a shake flask to a Stirred-Tank fermenter. The maximal lycopene production of 183.3 mg/L was obtained in 7.5-L Stirred-Tank fermenter, and then the mated fermentation process was successfully step-wise scaled-up from 7.5- to 200-L Stirred-Tank fermenter. The comparability of the fermentation process was well controlled and the lycopene production was maintained during the process scale-up. Furthermore, with the integrated addition of 150 μmol/L abscisic acid on day 3, 0.5 g/L leucine and 0.1 g/L penicillin on day 4, the highest lycopene production of 270.3 mg/L was achieved in the mated fermentation of B. trispora in Stirred-Tank fermenter.

Suzanne M Kresta - One of the best experts on this subject based on the ideXlab platform.

  • Three‐dimensional wall jets: Axial flow in a Stirred Tank
    Aiche Journal, 2001
    Co-Authors: Kevin J. Bittorf, Suzanne M Kresta
    Abstract:

    In this article, the flow at the wall of a Stirred Tank is compared with turbulent wall jet behavior. Agreement is shown to be very good. For all three axial impellers studied, the wall jet is three dimensional along the wall and baffle of the Tank. The expansion of the jet is linear, and the maximum velocity decays with 1/z, where z is the dimensionless axial distance. These wall-jet characteristics help further the fundamental understanding and modeling of the bulk flow in a Stirred Tank and provide meaningful test data for CFD validation.

  • Three-dimensional wall jets: Axial flow in a Stirred Tank
    AIChE Journal, 2001
    Co-Authors: Kevin J. Bittorf, Suzanne M Kresta
    Abstract:

    In this article, the flow at the wall of a Stirred Tank is compared with turbulent wall jet behavior. Agreement is shown to be very good. For all three axial impellers studied, the wall jet is three dimensional along the wall and baffle of the Tank. The expansion of the jet is linear, and the maximum velocity decays with 1/z, where z is the dimensionless axial distance. These wall-jet characteristics help further the fundamental understanding and modeling of the bulk flow in a Stirred Tank and provide meaningful test data for CFD validation.

  • Internal annular wall jets: Radial flow in a Stirred Tank
    AIChE Journal, 2001
    Co-Authors: Suzanne M Kresta, Kevin J. Bittorf, D.j. Wilson
    Abstract:

    Similarity solutions are derived for internal annular wall jets and compared with experimentally determined velocity profiles at the wall of a Stirred Tank with radial flow. The first similarity solution considers a geometry where the thickness of the wall jet is small relative to the diameter of the cylinder and the fluid flows relative to a free stream velocity of zero. In the second solution, the free stream velocity opposes the direction of the jet flow, as is observed in the recirculating flow in a Stirred Tank. The internal annular wall jet model agrees very well with the flow at the wall of a Stirred Tank agitated with a Rushton turbine. The free stream counter flow is driven by the wall jet, and a single velocity and length scale define the self-similar velocity profiles for the flow in the jet and in the recirculating flow. Both the analytical model and experimentally observed expansion rates are linear and the maximum velocity in the jet decays as [U ∝ (z/T)−0.5], where (z/T) is the dimensionless streamwise distance. This decay can be contrasted with the faster decay [U ∝ (z/T)−1] observed in the 3-D wall jets produced by axial impellers. These results provide a basis for validating computational fluid dynamic simulations in Stirred Tanks and for estimating the largest length scales of turbulence in the bulk of the Tank.

Kevin J. Bittorf - One of the best experts on this subject based on the ideXlab platform.

  • Three‐dimensional wall jets: Axial flow in a Stirred Tank
    Aiche Journal, 2001
    Co-Authors: Kevin J. Bittorf, Suzanne M Kresta
    Abstract:

    In this article, the flow at the wall of a Stirred Tank is compared with turbulent wall jet behavior. Agreement is shown to be very good. For all three axial impellers studied, the wall jet is three dimensional along the wall and baffle of the Tank. The expansion of the jet is linear, and the maximum velocity decays with 1/z, where z is the dimensionless axial distance. These wall-jet characteristics help further the fundamental understanding and modeling of the bulk flow in a Stirred Tank and provide meaningful test data for CFD validation.

  • Three-dimensional wall jets: Axial flow in a Stirred Tank
    AIChE Journal, 2001
    Co-Authors: Kevin J. Bittorf, Suzanne M Kresta
    Abstract:

    In this article, the flow at the wall of a Stirred Tank is compared with turbulent wall jet behavior. Agreement is shown to be very good. For all three axial impellers studied, the wall jet is three dimensional along the wall and baffle of the Tank. The expansion of the jet is linear, and the maximum velocity decays with 1/z, where z is the dimensionless axial distance. These wall-jet characteristics help further the fundamental understanding and modeling of the bulk flow in a Stirred Tank and provide meaningful test data for CFD validation.

  • Internal annular wall jets: Radial flow in a Stirred Tank
    AIChE Journal, 2001
    Co-Authors: Suzanne M Kresta, Kevin J. Bittorf, D.j. Wilson
    Abstract:

    Similarity solutions are derived for internal annular wall jets and compared with experimentally determined velocity profiles at the wall of a Stirred Tank with radial flow. The first similarity solution considers a geometry where the thickness of the wall jet is small relative to the diameter of the cylinder and the fluid flows relative to a free stream velocity of zero. In the second solution, the free stream velocity opposes the direction of the jet flow, as is observed in the recirculating flow in a Stirred Tank. The internal annular wall jet model agrees very well with the flow at the wall of a Stirred Tank agitated with a Rushton turbine. The free stream counter flow is driven by the wall jet, and a single velocity and length scale define the self-similar velocity profiles for the flow in the jet and in the recirculating flow. Both the analytical model and experimentally observed expansion rates are linear and the maximum velocity in the jet decays as [U ∝ (z/T)−0.5], where (z/T) is the dimensionless streamwise distance. This decay can be contrasted with the faster decay [U ∝ (z/T)−1] observed in the 3-D wall jets produced by axial impellers. These results provide a basis for validating computational fluid dynamic simulations in Stirred Tanks and for estimating the largest length scales of turbulence in the bulk of the Tank.

R. Saravanathamizhan - One of the best experts on this subject based on the ideXlab platform.

  • Residence time distribution in continuous Stirred Tank electrochemical reactor
    Chemical Engineering Journal, 2008
    Co-Authors: R. Saravanathamizhan, R. Paranthaman, Natesan Balasubramanian, C. Ahmed Basha
    Abstract:

    The aim of the present investigation is to study the electrolyte flow characteristics in a continuous Stirred Tank electrochemical reactor (CSTER) using residence time distribution (RTD). The flow behavior of electrolyte has been experimented using pulse tracer technique and the residence time distribution curves at various operating conditions are critically analyzed. A three-parameter model has been developed to explain the flow characteristic of electrolyte in a continuous Stirred Tank electrochemical reactor and the model simulations are validated with the experimental observations.

  • Tanks in series model for continuous Stirred Tank electrochemical reactor
    Industrial & Engineering Chemistry Research, 2008
    Co-Authors: R. Saravanathamizhan, R. Paranthaman, Natesan Balasubramanian, Ahmed C Basha
    Abstract:

    It is attempted in the present investigation to study the residence time distribution of electrolyte in a continuous Stirred Tank electrochemical reactor. The electrolyte flow behavior has been experimented on by using both pulse and step input techniques. The exit age distribution curves obtained under various operating conditions are critically analyzed. A theoretical model based on Tanks in series has been developed to describe the electrolyte flow behavior inside the continuous Stirred Tank electrochemical reactor, and the model simulations are validated with experimental observations.

C. Ahmed Basha - One of the best experts on this subject based on the ideXlab platform.