The Experts below are selected from a list of 285 Experts worldwide ranked by ideXlab platform
Jeffrey H. Peters - One of the best experts on this subject based on the ideXlab platform.
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Bile Salts at Low pH Cause Dilation of Intercellular Spaces in In Vitro Stratified Primary Esophageal Cells, Possibly by Modulating Wnt Signaling.
Journal of Gastrointestinal Surgery, 2015Co-Authors: Sayak Ghatak, Marie Reveiller, Liana Toia, Andrei I. Ivanov, Tony E. Godfrey, Zhongren Zhou, Eileen M. Redmond, Jeffrey H. PetersAbstract:Background The presence of dilated intercellular spaces in the stratified squamous lining of the esophagus is the pathognomonic feature of reflux esophagitis secondary to gastroesophageal reflux disease (GERD). In addition to Stomach Acid, bile salts are major constituents of gastroesophageal refluxate. The aim of our study was to determine the effect of bile salts cocktail at different pHs on epithelial junctions in an in vitro transwell model of stratified esophageal squamous epithelium.
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Bile Acid at Low pH Reduces Squamous Differentiation and Activates EGFR Signaling in Esophageal Squamous Cells in 3-D Culture
Journal of Gastrointestinal Surgery, 2013Co-Authors: Sayak Ghatak, Marie Reveiller, Liana Toia, Tony E. Godfrey, Andrei Ivanov, Jeffrey H. PetersAbstract:Background Barrett's esophagus is a preneoplastic metaplasia in which the normal squamous epithelium of the esophagus changes to an intestinal, columnar phenotype due to long-term gastro-esophageal reflux. The major components of this reflux are bile and Stomach Acid. Previous in vitro studies on the effect of bile and Acid on esophageal cells have predominantly relied on transformed esophageal squamous cells or cancer cells grown in monolayer culture. Discussion In this study, we expanded our previous work using an immortalized primary esophageal squamous cell line (EPC1). We demonstrate that EPC1 cells form a multi-layer, stratified epithelium when grown on polyester transwell filters in media supplemented with calcium. When exposed to short pulses of bile and pH 5, but not either condition alone, EPC1 cells demonstrate a reduction in stratification layers and reduced expression of squamous epithelium-specific genes. Bile at pH 5 also causes activation of epidermal growth factor receptor and down-stream pathways. Blocking epidermal growth factor receptor activation partially attenuates the effects of bile Acid and pH 5. These results suggest that bile at low pH, but not bile or low pH alone, promotes loss of differentiation status of stratified squamous esophageal epithelium in vitro, possibly by initiating a mucosal repair response through epidermal growth factor activation.
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Bile Acid at Low pH Reduces Squamous Differentiation and Activates EGFR Signaling in Esophageal Squamous Cells in 3-D Culture
Journal of Gastrointestinal Surgery, 2013Co-Authors: Sayak Ghatak, Marie Reveiller, Liana Toia, Andrei I. Ivanov, Tony E. Godfrey, Jeffrey H. PetersAbstract:Background Barrett's esophagus is a preneoplastic metaplasia in which the normal squamous epithelium of the esophagus changes to an intestinal, columnar phenotype due to long-term gastro-esophageal reflux. The major components of this reflux are bile and Stomach Acid. Previous in vitro studies on the effect of bile and Acid on esophageal cells have predominantly relied on transformed esophageal squamous cells or cancer cells grown in monolayer culture.
Sayak Ghatak - One of the best experts on this subject based on the ideXlab platform.
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Bile Salts at Low pH Cause Dilation of Intercellular Spaces in In Vitro Stratified Primary Esophageal Cells, Possibly by Modulating Wnt Signaling.
Journal of Gastrointestinal Surgery, 2015Co-Authors: Sayak Ghatak, Marie Reveiller, Liana Toia, Andrei I. Ivanov, Tony E. Godfrey, Zhongren Zhou, Eileen M. Redmond, Jeffrey H. PetersAbstract:Background The presence of dilated intercellular spaces in the stratified squamous lining of the esophagus is the pathognomonic feature of reflux esophagitis secondary to gastroesophageal reflux disease (GERD). In addition to Stomach Acid, bile salts are major constituents of gastroesophageal refluxate. The aim of our study was to determine the effect of bile salts cocktail at different pHs on epithelial junctions in an in vitro transwell model of stratified esophageal squamous epithelium.
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Bile Acid at Low pH Reduces Squamous Differentiation and Activates EGFR Signaling in Esophageal Squamous Cells in 3-D Culture
Journal of Gastrointestinal Surgery, 2013Co-Authors: Sayak Ghatak, Marie Reveiller, Liana Toia, Tony E. Godfrey, Andrei Ivanov, Jeffrey H. PetersAbstract:Background Barrett's esophagus is a preneoplastic metaplasia in which the normal squamous epithelium of the esophagus changes to an intestinal, columnar phenotype due to long-term gastro-esophageal reflux. The major components of this reflux are bile and Stomach Acid. Previous in vitro studies on the effect of bile and Acid on esophageal cells have predominantly relied on transformed esophageal squamous cells or cancer cells grown in monolayer culture. Discussion In this study, we expanded our previous work using an immortalized primary esophageal squamous cell line (EPC1). We demonstrate that EPC1 cells form a multi-layer, stratified epithelium when grown on polyester transwell filters in media supplemented with calcium. When exposed to short pulses of bile and pH 5, but not either condition alone, EPC1 cells demonstrate a reduction in stratification layers and reduced expression of squamous epithelium-specific genes. Bile at pH 5 also causes activation of epidermal growth factor receptor and down-stream pathways. Blocking epidermal growth factor receptor activation partially attenuates the effects of bile Acid and pH 5. These results suggest that bile at low pH, but not bile or low pH alone, promotes loss of differentiation status of stratified squamous esophageal epithelium in vitro, possibly by initiating a mucosal repair response through epidermal growth factor activation.
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Bile Acid at Low pH Reduces Squamous Differentiation and Activates EGFR Signaling in Esophageal Squamous Cells in 3-D Culture
Journal of Gastrointestinal Surgery, 2013Co-Authors: Sayak Ghatak, Marie Reveiller, Liana Toia, Andrei I. Ivanov, Tony E. Godfrey, Jeffrey H. PetersAbstract:Background Barrett's esophagus is a preneoplastic metaplasia in which the normal squamous epithelium of the esophagus changes to an intestinal, columnar phenotype due to long-term gastro-esophageal reflux. The major components of this reflux are bile and Stomach Acid. Previous in vitro studies on the effect of bile and Acid on esophageal cells have predominantly relied on transformed esophageal squamous cells or cancer cells grown in monolayer culture.
Ronald R Dubreuil - One of the best experts on this subject based on the ideXlab platform.
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copper cells and Stomach Acid secretion in the drosophila midgut
The International Journal of Biochemistry & Cell Biology, 2004Co-Authors: Ronald R DubreuilAbstract:Abstract Copper cells in the Drosophila larval midgut were originally named for their ability to accumulate dietary copper. Recent studies have uncovered a number of intriguing similarities between copper cells and the Acid-producing gastric parietal cells of the mammalian Stomach. In addition to their shared roles in Stomach Acidification, they share a peculiar invaginated morphology in which the apical cell surface is buried deep within the cytoplasm. These shared properties of morphology and function portend the identification of shared molecular mechanisms that account for their specialized roles in digestive physiology. Cell facts: • Approximately 100 copper cells in larval middle midgut. • Orange fluorescence after copper feeding. • Specification during development is controlled by the labial gene. • Apical surface is arranged as a flask-shaped invagination. • Responsible for Stomach Acidification.
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differential effects of a labial mutation on the development structure and function of Stomach Acid secreting cells in drosophila melanogaster larvae and adults
Cell and Tissue Research, 2001Co-Authors: Ronald R Dubreuil, Tatyana A Grushko, Otto BaumannAbstract:The differentiation of copper cells, which secrete Stomach Acid in Drosophila larvae, has been shown previously to be sensitive to the labial k3 mutation. Here we found that Stomach Acid secretion in adults was insensitive to lab k3 . The basis for this stage-specific effect was elucidated by characterizing the development, structure, and function of the adult midgut. First, we demonstrated by copper-dependent fluorescence and morphology that copper cells were present in the adult Stomach. Fine-structure analysis of adult copper cells led to the identification of a previously unrecognized plasma membrane domain: apicolateral contacts between copper cells and their neighbors consisted of smooth septate junctions that were enriched in αβ-spectrin and ankyrin. Second, we demonstrated that adult copper cells were present in lab k3 /lab vd1 (conditional/null) adults. The labial protein was expressed in adult lab k3 /lab vd1 copper cells, but not in larvae. Thus the lab k3 mutation had a stage-specific effect on midgut labial expression, but did not appear to affect protein function. Surprisingly, Stomach Acidification was dispensable during larval development, since lab k3 /lab vd1 mutant larvae that lacked midgut Acidification developed into fertile adults.
Tony E. Godfrey - One of the best experts on this subject based on the ideXlab platform.
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Bile Salts at Low pH Cause Dilation of Intercellular Spaces in In Vitro Stratified Primary Esophageal Cells, Possibly by Modulating Wnt Signaling.
Journal of Gastrointestinal Surgery, 2015Co-Authors: Sayak Ghatak, Marie Reveiller, Liana Toia, Andrei I. Ivanov, Tony E. Godfrey, Zhongren Zhou, Eileen M. Redmond, Jeffrey H. PetersAbstract:Background The presence of dilated intercellular spaces in the stratified squamous lining of the esophagus is the pathognomonic feature of reflux esophagitis secondary to gastroesophageal reflux disease (GERD). In addition to Stomach Acid, bile salts are major constituents of gastroesophageal refluxate. The aim of our study was to determine the effect of bile salts cocktail at different pHs on epithelial junctions in an in vitro transwell model of stratified esophageal squamous epithelium.
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Bile Acid at Low pH Reduces Squamous Differentiation and Activates EGFR Signaling in Esophageal Squamous Cells in 3-D Culture
Journal of Gastrointestinal Surgery, 2013Co-Authors: Sayak Ghatak, Marie Reveiller, Liana Toia, Tony E. Godfrey, Andrei Ivanov, Jeffrey H. PetersAbstract:Background Barrett's esophagus is a preneoplastic metaplasia in which the normal squamous epithelium of the esophagus changes to an intestinal, columnar phenotype due to long-term gastro-esophageal reflux. The major components of this reflux are bile and Stomach Acid. Previous in vitro studies on the effect of bile and Acid on esophageal cells have predominantly relied on transformed esophageal squamous cells or cancer cells grown in monolayer culture. Discussion In this study, we expanded our previous work using an immortalized primary esophageal squamous cell line (EPC1). We demonstrate that EPC1 cells form a multi-layer, stratified epithelium when grown on polyester transwell filters in media supplemented with calcium. When exposed to short pulses of bile and pH 5, but not either condition alone, EPC1 cells demonstrate a reduction in stratification layers and reduced expression of squamous epithelium-specific genes. Bile at pH 5 also causes activation of epidermal growth factor receptor and down-stream pathways. Blocking epidermal growth factor receptor activation partially attenuates the effects of bile Acid and pH 5. These results suggest that bile at low pH, but not bile or low pH alone, promotes loss of differentiation status of stratified squamous esophageal epithelium in vitro, possibly by initiating a mucosal repair response through epidermal growth factor activation.
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Bile Acid at Low pH Reduces Squamous Differentiation and Activates EGFR Signaling in Esophageal Squamous Cells in 3-D Culture
Journal of Gastrointestinal Surgery, 2013Co-Authors: Sayak Ghatak, Marie Reveiller, Liana Toia, Andrei I. Ivanov, Tony E. Godfrey, Jeffrey H. PetersAbstract:Background Barrett's esophagus is a preneoplastic metaplasia in which the normal squamous epithelium of the esophagus changes to an intestinal, columnar phenotype due to long-term gastro-esophageal reflux. The major components of this reflux are bile and Stomach Acid. Previous in vitro studies on the effect of bile and Acid on esophageal cells have predominantly relied on transformed esophageal squamous cells or cancer cells grown in monolayer culture.
Marie Reveiller - One of the best experts on this subject based on the ideXlab platform.
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Bile Salts at Low pH Cause Dilation of Intercellular Spaces in In Vitro Stratified Primary Esophageal Cells, Possibly by Modulating Wnt Signaling.
Journal of Gastrointestinal Surgery, 2015Co-Authors: Sayak Ghatak, Marie Reveiller, Liana Toia, Andrei I. Ivanov, Tony E. Godfrey, Zhongren Zhou, Eileen M. Redmond, Jeffrey H. PetersAbstract:Background The presence of dilated intercellular spaces in the stratified squamous lining of the esophagus is the pathognomonic feature of reflux esophagitis secondary to gastroesophageal reflux disease (GERD). In addition to Stomach Acid, bile salts are major constituents of gastroesophageal refluxate. The aim of our study was to determine the effect of bile salts cocktail at different pHs on epithelial junctions in an in vitro transwell model of stratified esophageal squamous epithelium.
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Bile Acid at Low pH Reduces Squamous Differentiation and Activates EGFR Signaling in Esophageal Squamous Cells in 3-D Culture
Journal of Gastrointestinal Surgery, 2013Co-Authors: Sayak Ghatak, Marie Reveiller, Liana Toia, Tony E. Godfrey, Andrei Ivanov, Jeffrey H. PetersAbstract:Background Barrett's esophagus is a preneoplastic metaplasia in which the normal squamous epithelium of the esophagus changes to an intestinal, columnar phenotype due to long-term gastro-esophageal reflux. The major components of this reflux are bile and Stomach Acid. Previous in vitro studies on the effect of bile and Acid on esophageal cells have predominantly relied on transformed esophageal squamous cells or cancer cells grown in monolayer culture. Discussion In this study, we expanded our previous work using an immortalized primary esophageal squamous cell line (EPC1). We demonstrate that EPC1 cells form a multi-layer, stratified epithelium when grown on polyester transwell filters in media supplemented with calcium. When exposed to short pulses of bile and pH 5, but not either condition alone, EPC1 cells demonstrate a reduction in stratification layers and reduced expression of squamous epithelium-specific genes. Bile at pH 5 also causes activation of epidermal growth factor receptor and down-stream pathways. Blocking epidermal growth factor receptor activation partially attenuates the effects of bile Acid and pH 5. These results suggest that bile at low pH, but not bile or low pH alone, promotes loss of differentiation status of stratified squamous esophageal epithelium in vitro, possibly by initiating a mucosal repair response through epidermal growth factor activation.
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Bile Acid at Low pH Reduces Squamous Differentiation and Activates EGFR Signaling in Esophageal Squamous Cells in 3-D Culture
Journal of Gastrointestinal Surgery, 2013Co-Authors: Sayak Ghatak, Marie Reveiller, Liana Toia, Andrei I. Ivanov, Tony E. Godfrey, Jeffrey H. PetersAbstract:Background Barrett's esophagus is a preneoplastic metaplasia in which the normal squamous epithelium of the esophagus changes to an intestinal, columnar phenotype due to long-term gastro-esophageal reflux. The major components of this reflux are bile and Stomach Acid. Previous in vitro studies on the effect of bile and Acid on esophageal cells have predominantly relied on transformed esophageal squamous cells or cancer cells grown in monolayer culture.
